FastQCFastQC Report
Thu 26 May 2016
SRR1364211_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364211_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2211151
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60170.2721207190282346No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55760.2521763552104764No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30840.13947487077996937No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36650.070.835761
GTATCAA63800.048.904711
ATCAACG80550.038.7871743
TCAACGC81000.038.5716934
CAACGCA82200.038.0008665
TATCAAC83200.037.838732
AACGCAG85950.036.5497326
TGGTATC17550.033.2317472
GTACATG77400.031.9261971
ACGCAGA99350.031.6178827
TACATGG78700.030.9280682
ACATGGG77600.030.752263
CGCAGAG101450.030.611548
GTGGTAT21350.029.5629671
GCAGAGT113750.026.7261379
CATGGGG48750.026.0015564
CAGAGTA109050.025.2593410-11
GAGTACT73100.025.2296412-13
GTACTTT78400.023.6378914-15
AGAGTAC104300.023.21543310-11
Produced by FastQC (version 0.11.5)