FastQCFastQC Report
Thu 26 May 2016
SRR1364210_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364210_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1984549
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35330.17802533472340568No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20950.050.551741
GTATCAA33900.045.8079571
ATCAACG37050.039.0178643
GTACATG92100.038.11471
TCAACGC38350.037.694274
CAACGCA38550.037.4996575
AACGCAG40400.036.3595666
TACATGG96150.035.640232
ACATGGG92200.035.616693
TATCAAC43600.033.9766162
CATGGGG43400.030.9779854
ACGCAGA48550.029.5202457
CGCAGAG49900.028.7216018
GGTCGAC1055.925087E-428.3186727
CATGGGA57850.028.1761514
GAGTACA67500.024.4160921
AGTACAT64100.024.1314052
ATGGGAG29550.023.5544915
GCAGAGT61150.022.3695059
ATGGGGA28300.021.4416965
Produced by FastQC (version 0.11.5)