##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364210_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1984549 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.04124312375255 33.0 31.0 34.0 27.0 34.0 2 31.0083394262374 33.0 31.0 34.0 27.0 34.0 3 31.1312640806551 34.0 31.0 34.0 27.0 34.0 4 34.4767506370465 37.0 35.0 37.0 31.0 37.0 5 34.59044649439243 37.0 35.0 37.0 31.0 37.0 6 34.6452967399646 37.0 35.0 37.0 31.0 37.0 7 34.56364594676171 37.0 35.0 37.0 31.0 37.0 8 34.5163495585143 37.0 35.0 37.0 31.0 37.0 9 35.99117330940178 39.0 37.0 39.0 31.0 39.0 10-11 35.970768169493425 39.0 37.0 39.0 31.0 39.0 12-13 35.92649513819009 39.0 37.0 39.0 30.5 39.0 14-15 37.247242068600976 40.0 38.0 41.0 31.0 41.0 16-17 37.28846276912286 40.0 38.0 41.0 31.0 41.0 18-19 37.19682885129065 40.0 38.0 41.0 30.5 41.0 20-21 37.18767614203529 40.0 38.0 41.0 30.0 41.0 22-23 37.23779483398999 40.0 38.0 41.0 30.0 41.0 24-25 37.10621708005194 40.0 37.0 41.0 30.0 41.0 26-27 37.1381283606502 40.0 37.0 41.0 30.0 41.0 28-29 37.235693348967445 40.0 37.0 41.0 30.0 41.0 30-31 37.134859607900836 40.0 37.0 41.0 30.0 41.0 32-33 36.96346197549166 40.0 37.0 41.0 30.0 41.0 34-35 36.91827211119504 40.0 37.0 41.0 30.0 41.0 36-37 36.941451433046 40.0 37.0 41.0 30.0 41.0 38-39 36.779510609211464 40.0 37.0 41.0 29.5 41.0 40-41 36.78125332254331 40.0 37.0 41.0 30.0 41.0 42-43 36.71584727814732 40.0 37.0 41.0 30.0 41.0 44-45 36.71065743400642 40.0 36.5 41.0 30.0 41.0 46-47 36.63785651047165 40.0 36.0 41.0 30.0 41.0 48-49 36.55373387102057 40.0 36.0 41.0 30.0 41.0 50-51 36.447180946401424 40.0 36.0 41.0 29.5 41.0 52-53 36.3088968828686 40.0 35.5 41.0 29.0 41.0 54-55 36.13150065833598 39.5 35.0 41.0 29.0 41.0 56-57 35.95193164794621 39.0 35.0 41.0 29.0 41.0 58-59 35.738424700020005 39.0 35.0 41.0 29.0 41.0 60-61 35.509421788023374 38.5 35.0 41.0 28.0 41.0 62-63 35.236141057741584 38.0 34.5 40.0 28.0 41.0 64-65 34.93830613403851 37.5 34.0 40.0 28.0 41.0 66-67 34.6367537410263 37.0 34.0 40.0 28.0 41.0 68-69 34.40541478189755 37.0 34.0 39.5 28.0 41.0 70-71 34.232411495004655 36.0 34.0 39.0 28.0 41.0 72-73 33.91726004245801 36.0 34.0 39.0 28.0 41.0 74-75 33.574732848622034 35.0 34.0 38.5 27.0 40.0 76-77 33.23630356317733 35.0 34.0 37.0 27.0 39.0 78-79 32.88493103470864 35.0 33.0 37.0 26.0 39.0 80-81 32.57452675645701 35.0 33.0 36.0 26.0 39.0 82-83 32.297064723521565 35.0 33.0 36.0 26.0 37.0 84-85 32.04128419101771 35.0 33.0 35.5 26.0 37.0 86-87 31.826207364998297 35.0 33.0 35.0 25.0 37.0 88-89 31.64966574269519 35.0 33.0 35.0 25.0 36.0 90-91 31.49934569516802 35.0 33.0 35.0 25.0 36.0 92-93 31.367482989837995 35.0 33.0 35.0 25.0 36.0 94-95 31.235482973713424 35.0 33.0 35.0 24.0 35.5 96-97 31.138195378395796 35.0 32.5 35.0 24.0 35.0 98-99 31.059399389987348 35.0 32.0 35.0 23.5 35.0 100-101 30.95894633994928 35.0 32.0 35.0 23.0 35.0 102-103 29.972886534925568 34.0 30.5 35.0 19.5 35.0 104-105 30.65838384438983 34.5 32.0 35.0 20.0 35.0 106-107 30.762440987851647 35.0 32.0 35.0 20.0 35.0 108-109 30.720670792205183 35.0 32.0 35.0 19.5 35.0 110-111 30.64846093495298 35.0 32.0 35.0 19.0 35.0 112-113 30.79347448715048 35.0 33.0 35.0 19.5 35.0 114-115 30.782016216278862 35.0 33.0 35.0 19.0 35.0 116-117 30.690704789854017 35.0 33.0 35.0 18.0 35.0 118-119 30.44632256497572 35.0 32.5 35.0 7.0 35.0 120-121 30.38300062129985 35.0 32.0 35.0 2.0 35.0 122-123 30.24704932959579 35.0 32.0 35.0 2.0 35.0 124-125 29.383115508863725 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5793709659308242 1101 2 0.6250925389287225 1101 3 0.6306918835974002 1101 4 0.6356339863367921 1101 5 0.5127951465394318 1101 6 0.5189260039039851 1101 7 0.5685183386769168 1101 8 0.5539086609117021 1101 9 0.5201828008044487 1101 10-11 0.5761706583510176 1101 12-13 0.5466814927857868 1101 14-15 0.6581072048725289 1101 16-17 0.5817509076536922 1101 18-19 0.6747151589381915 1101 20-21 0.7672662321440029 1101 22-23 0.7698687343277584 1101 24-25 0.8991962143225791 1101 26-27 0.9488237486419138 1101 28-29 0.9496772266864966 1101 30-31 0.9591643479732213 1101 32-33 1.1028432983918748 1101 34-35 1.0629493533563945 1101 36-37 0.9930417567369219 1101 38-39 1.0209274680489955 1101 40-41 0.9806010718204305 1101 42-43 0.8236791501756642 1101 44-45 0.8259812738865762 1101 46-47 0.7221043851960331 1101 48-49 0.6835009354933064 1101 50-51 0.5930763233585097 1101 52-53 0.5577053841567974 1101 54-55 0.6259495705731624 1101 56-57 0.5651053894652804 1101 58-59 0.6416261565564909 1101 60-61 0.5859810955520999 1101 62-63 0.6452144571009626 1101 64-65 0.5899806199201976 1101 66-67 0.5489780011821068 1101 68-69 0.534721114555424 1101 70-71 0.5398958011455406 1101 72-73 0.5426303525130294 1101 74-75 0.524507502159409 1101 76-77 0.5699366346752512 1101 78-79 0.47760137821005344 1101 80-81 0.4780972317762391 1101 82-83 0.4843019618457305 1101 84-85 0.39509833975082387 1101 86-87 0.37776383039319583 1101 88-89 0.4319825927130374 1101 90-91 0.4343297355565383 1101 92-93 0.40166957105303425 1101 94-95 0.38496825732569206 1101 96-97 0.4896791380696115 1101 98-99 0.4626607366125235 1101 100-101 0.40119008949553603 1101 102-103 0.28793839277117783 1101 104-105 0.479958519034664 1101 106-107 0.49414584938440953 1101 108-109 0.43013819980379964 1101 110-111 0.3343683204219019 1101 112-113 0.3520024038443772 1101 114-115 0.3561045330555821 1101 116-117 0.31826463148538053 1101 118-119 0.30824377246368684 1101 120-121 0.3198811245353923 1101 122-123 0.2448670796278627 1101 124-125 0.20611856581450638 1102 1 0.13491801311109697 1102 2 0.1967293723160921 1102 3 0.06355949902500413 1102 4 0.2744057273732494 1102 5 0.2577788285409426 1102 6 0.24594033371804613 1102 7 0.36291925303990524 1102 8 0.2847412888348444 1102 9 0.29601841226578074 1102 10-11 0.3686593328905232 1102 12-13 0.3085024855407781 1102 14-15 0.3697812284103108 1102 16-17 0.3402114776419509 1102 18-19 0.4663034735608136 1102 20-21 0.48356562294713257 1102 22-23 0.449677560806947 1102 24-25 0.6901953905380367 1102 26-27 0.6885959526629435 1102 28-29 0.5577561813253666 1102 30-31 0.6055716759971617 1102 32-33 0.7513806708345783 1102 34-35 0.5932779967106754 1102 36-37 0.5312105455213469 1102 38-39 0.608566032266971 1102 40-41 0.49127702929649075 1102 42-43 0.3751996192660769 1102 44-45 0.28594465142842296 1102 46-47 0.2832652780119531 1102 48-49 0.26801794923019173 1102 50-51 0.21581908433609698 1102 52-53 0.14509624532072252 1102 54-55 0.15359000186789018 1102 56-57 0.2376931073802595 1102 58-59 0.3097371164231717 1102 60-61 0.2766228148774843 1102 62-63 0.21707041969887086 1102 64-65 0.1607137503140592 1102 66-67 0.11720887026848459 1102 68-69 0.16505354252474547 1102 70-71 0.09275917780430376 1102 72-73 0.10504798982832142 1102 74-75 0.01753567357218344 1102 76-77 0.05266081319302174 1102 78-79 0.08619075126333797 1102 80-81 0.1498018728496575 1102 82-83 0.12863538390725893 1102 84-85 0.1546642696370384 1102 86-87 0.15720624306004893 1102 88-89 0.15897875637492476 1102 90-91 0.08213686652819163 1102 92-93 0.065541131311047 1102 94-95 0.09487007179629359 1102 96-97 0.11694773093712385 1102 98-99 0.10712106943157096 1102 100-101 0.19361940865590554 1102 102-103 0.11969837753179391 1102 104-105 0.12182191209342719 1102 106-107 0.15705794665406003 1102 108-109 0.12561306175996734 1102 110-111 0.09921769905119149 1102 112-113 0.18943054346639343 1102 114-115 0.16499673838860573 1102 116-117 0.1507170746436195 1102 118-119 0.1270484522663331 1102 120-121 0.14564728831082263 1102 122-123 0.16144773040503324 1102 124-125 0.05164142930470206 1103 1 0.33874176626520835 1103 2 0.10875929975194865 1103 3 0.44439552382629444 1103 4 0.5379989968347019 1103 5 0.4075173974877302 1103 6 0.394095969887033 1103 7 0.456024031616181 1103 8 0.380828081292222 1103 9 0.4932403833245118 1103 10-11 0.5237592577825936 1103 12-13 0.43828543206223713 1103 14-15 0.5785753663750199 1103 16-17 0.45754525463046747 1103 18-19 0.6195596976934823 1103 20-21 0.648818319538826 1103 22-23 0.624916055610278 1103 24-25 0.8501203067197594 1103 26-27 0.8107538167525519 1103 28-29 0.733965834924696 1103 30-31 0.6965642700900361 1103 32-33 0.9242988369472869 1103 34-35 0.9141112245553416 1103 36-37 0.7273715417201316 1103 38-39 0.9190877731795695 1103 40-41 0.8084306316440077 1103 42-43 0.7844125773732102 1103 44-45 0.7641161530927292 1103 46-47 0.6927051271691909 1103 48-49 0.6751280058565143 1103 50-51 0.6815839736091718 1103 52-53 0.5821421166149037 1103 54-55 0.5134943025503702 1103 56-57 0.5752292899925848 1103 58-59 0.5702667539158952 1103 60-61 0.5833821066205473 1103 62-63 0.5883350660466107 1103 64-65 0.5552004304109488 1103 66-67 0.5467678191432555 1103 68-69 0.5876076220426967 1103 70-71 0.6259960643575724 1103 72-73 0.6080952258444441 1103 74-75 0.5388437209709593 1103 76-77 0.5231622641142692 1103 78-79 0.5636909833384038 1103 80-81 0.5322406622979017 1103 82-83 0.4354221629826469 1103 84-85 0.4600262283747014 1103 86-87 0.5205697883472418 1103 88-89 0.5144116637751672 1103 90-91 0.5089805371479699 1103 92-93 0.45840961527616386 1103 94-95 0.5319920757674907 1103 96-97 0.5699755570387559 1103 98-99 0.6190855938946918 1103 100-101 0.5978070675670466 1103 102-103 0.5115351220671833 1103 104-105 0.5273713392598331 1103 106-107 0.5861217618716736 1103 108-109 0.5259122878951139 1103 110-111 0.5313540072854579 1103 112-113 0.6219563158544048 1103 114-115 0.5494943651950273 1103 116-117 0.5048227537917036 1103 118-119 0.31041722979133723 1103 120-121 0.3711449235563329 1103 122-123 0.4373519491077502 1103 124-125 0.30524751413205564 1104 1 0.2703662673714433 1104 2 0.313526304669292 1104 3 0.2750984123302125 1104 4 0.2786991114751203 1104 5 0.24856781281117435 1104 6 0.26523890560806507 1104 7 0.27158750867061343 1104 8 0.36338546529794513 1104 9 0.31652779433169087 1104 10-11 0.3932512839882776 1104 12-13 0.39912617024071295 1104 14-15 0.4013940676965504 1104 16-17 0.4101201040526874 1104 18-19 0.5651531060109178 1104 20-21 0.5380009371019625 1104 22-23 0.5946876182641532 1104 24-25 0.7283454546512758 1104 26-27 0.7286596254211801 1104 28-29 0.5438913281006208 1104 30-31 0.5917814584037089 1104 32-33 0.7548801031143668 1104 34-35 0.6618032945190819 1104 36-37 0.6597844910789306 1104 38-39 0.7598649369584933 1104 40-41 0.6981006836527683 1104 42-43 0.5892292826471461 1104 44-45 0.5064137791948298 1104 46-47 0.43410437827329673 1104 48-49 0.3840028214442057 1104 50-51 0.22970583883626006 1104 52-53 0.30578308889422345 1104 54-55 0.26762798528654486 1104 56-57 0.26048964330814073 1104 58-59 0.2966684173834011 1104 60-61 0.2084008313805228 1104 62-63 0.19700016432265954 1104 64-65 0.22122171307944427 1104 66-67 0.24384644943120293 1104 68-69 0.28455571062865204 1104 70-71 0.28931064457798783 1104 72-73 0.26784702176748 1104 74-75 0.2917544465134654 1104 76-77 0.20786193786634044 1104 78-79 0.2753589187995331 1104 80-81 0.2792649612476126 1104 82-83 0.2704483445681589 1104 84-85 0.288005107318007 1104 86-87 0.33169945488821284 1104 88-89 0.3108598189905507 1104 90-91 0.2789912805528374 1104 92-93 0.3267975303987747 1104 94-95 0.32630011496260636 1104 96-97 0.2614971274896938 1104 98-99 0.21057217208126033 1104 100-101 0.27696049104385523 1104 102-103 0.16558460835900135 1104 104-105 0.17245166552666902 1104 106-107 0.19709727106700825 1104 108-109 0.12355287362923306 1104 110-111 0.03799409069983284 1104 112-113 0.17428942094649713 1104 114-115 0.2144822419466479 1104 116-117 0.15760716694709132 1104 118-119 0.020034538226997967 1104 120-121 0.0324000755234124 1104 122-123 0.07061836948179945 1104 124-125 0.09132947683659154 1105 1 0.2745637715997695 1105 2 0.2607565105184442 1105 3 0.22754756647243113 1105 4 0.29764422607334495 1105 5 0.18888725848633925 1105 6 0.11685645691624558 1105 7 0.13891776884267415 1105 8 -0.03374630435750703 1105 9 0.16072892008730122 1105 10-11 0.11986426022874497 1105 12-13 0.14712638406493284 1105 14-15 0.16939936706972247 1105 16-17 0.2113820522031986 1105 18-19 0.35610477788513606 1105 20-21 0.4004498408194266 1105 22-23 0.40521504170159517 1105 24-25 0.6328190040176906 1105 26-27 0.5841847661406305 1105 28-29 0.5257677993460774 1105 30-31 0.4914044728243212 1105 32-33 0.6409476734933577 1105 34-35 0.7098458397592751 1105 36-37 0.5503570583950363 1105 38-39 0.6145954606544564 1105 40-41 0.5836893166803279 1105 42-43 0.5258783200529038 1105 44-45 0.5163645514662605 1105 46-47 0.39351582229951276 1105 48-49 0.42177771077572146 1105 50-51 0.3286363806398569 1105 52-53 0.3662182885983398 1105 54-55 0.346948342122559 1105 56-57 0.4271413005421394 1105 58-59 0.3941026712524618 1105 60-61 0.26556726560164634 1105 62-63 0.39534548272810355 1105 64-65 0.3646929469800284 1105 66-67 0.38163593082079217 1105 68-69 0.30296433833949976 1105 70-71 0.49618388919675027 1105 72-73 0.4102103669028807 1105 74-75 0.38043442871404665 1105 76-77 0.34330729903663126 1105 78-79 0.3522727900195619 1105 80-81 0.3642694785249958 1105 82-83 0.37318341636301255 1105 84-85 0.384413823640692 1105 86-87 0.33698823316212767 1105 88-89 0.31080058390334386 1105 90-91 0.31883843476440177 1105 92-93 0.31539959197244727 1105 94-95 0.4061502164809525 1105 96-97 0.393194063446451 1105 98-99 0.33145846382995714 1105 100-101 0.3907424250164766 1105 102-103 0.3740680360189934 1105 104-105 0.30419362978142317 1105 106-107 0.40865345867753433 1105 108-109 0.379643492717765 1105 110-111 0.30612363928030817 1105 112-113 0.2671158592514722 1105 114-115 0.292646792203044 1105 116-117 0.27845276467246904 1105 118-119 0.1947455384234864 1105 120-121 0.2069413166285159 1105 122-123 0.18739673201209328 1105 124-125 0.2016011299096867 1106 1 0.4028052808644311 1106 2 0.41935632432794634 1106 3 0.3959135018366631 1106 4 0.3322726676065386 1106 5 0.4985799133085962 1106 6 0.29093943132813394 1106 7 0.35035988528277073 1106 8 0.3934393776680096 1106 9 0.5027628269471904 1106 10-11 0.5704179245923342 1106 12-13 0.4911434720904211 1106 14-15 0.5478175329811563 1106 16-17 0.5727605629035466 1106 18-19 0.6143137038127264 1106 20-21 0.673096475183165 1106 22-23 0.6049148656057639 1106 24-25 0.8206001124119666 1106 26-27 0.7521490179389758 1106 28-29 0.6917645260573053 1106 30-31 0.7077707764569041 1106 32-33 0.8701615420682884 1106 34-35 0.8369547368983703 1106 36-37 0.7477876856102483 1106 38-39 0.7214453922350543 1106 40-41 0.607098892779149 1106 42-43 0.5848433177348724 1106 44-45 0.5642536914755283 1106 46-47 0.5117946911735487 1106 48-49 0.41882665681786335 1106 50-51 0.45618061022994283 1106 52-53 0.4602571081954494 1106 54-55 0.4117523560606742 1106 56-57 0.42011288966681803 1106 58-59 0.4469417024636755 1106 60-61 0.4379556447722308 1106 62-63 0.4104068639026792 1106 64-65 0.4226428699614644 1106 66-67 0.4761811194040959 1106 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2216 78-79 -2.2996492332515572 2216 80-81 -2.3172605700078357 2216 82-83 -2.4114557969966413 2216 84-85 -2.348544976946762 2216 86-87 -2.2340670154835287 2216 88-89 -2.165036745202414 2216 90-91 -2.2197612942038916 2216 92-93 -2.2319977255917713 2216 94-95 -2.2076693204957465 2216 96-97 -2.3072589663702807 2216 98-99 -2.31137621655688 2216 100-101 -2.2586563044413595 2216 102-103 -2.3312465844555668 2216 104-105 -2.281367772191661 2216 106-107 -2.337017044903263 2216 108-109 -2.255891608481452 2216 110-111 -2.253149956850031 2216 112-113 -2.2692091972404853 2216 114-115 -2.2915365545846136 2216 116-117 -2.13328868696815 2216 118-119 -2.097997879493782 2216 120-121 -1.9936646953474515 2216 122-123 -2.047944335126612 2216 124-125 -1.8907230966287258 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 31484.0 3 6454.0 4 4719.0 5 3827.0 6 4174.0 7 4688.0 8 4544.0 9 4751.0 10 5338.0 11 6246.0 12 6718.0 13 6971.0 14 7095.0 15 7044.0 16 6424.0 17 5987.0 18 5222.0 19 5110.0 20 4806.0 21 5014.0 22 5645.0 23 8011.0 24 12854.0 25 19616.0 26 23724.0 27 21151.0 28 23165.0 29 28698.0 30 34422.0 31 41481.0 32 53779.0 33 71997.0 34 103385.0 35 161937.0 36 321215.0 37 629113.0 38 286608.0 39 1132.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.56001524734583 18.688032446997322 12.62262415695892 24.129328148697926 2 15.721425089245788 20.75699988959284 39.52858663206234 23.992988389099033 3 18.804527008292794 25.553903158319258 29.002495399894133 26.639074433493814 4 12.49812216371103 16.99111644803107 36.964791451786745 33.54596993647116 5 13.609970973111562 38.81007905516924 33.7759454911533 13.804004480565899 6 31.803756006708163 36.910062647144194 17.62721925694198 13.658962089205664 7 28.38338940843248 32.96092144228817 21.01775675080535 17.637932398474003 8 25.379015584901154 36.645252901288906 19.82803145702122 18.14770005678872 9 26.246725905232402 17.165729915274134 19.45434609310674 37.13319808638672 10-11 24.509512691104803 27.68324002175884 27.58699317025287 20.22025411688349 12-13 24.82084219474642 25.2439476370167 27.547910839718043 22.38729932851884 14-15 22.194396717783118 26.700591441049532 25.039628036921158 26.06538380424619 16-17 22.607375186982374 29.671044559061738 25.58311919227142 22.138461061684467 18-19 22.499170423591668 28.505656363121908 27.101032958056564 21.894140255229857 20-21 23.393341781063363 27.798491150419064 26.803585741394137 22.004581327123436 22-23 23.08961552165812 27.731386775969007 26.55117549910015 22.62782220327272 24-25 22.873242914337112 27.88325820813166 26.868198733663736 22.37530014386749 26-27 22.663438537322765 28.088328714820328 26.666312204087795 22.581920543769115 28-29 22.51810563060356 27.917580987468988 27.002138937455296 22.56217444447216 30-31 22.64831530742726 27.61928278520721 27.066272763349353 22.666129144016175 32-33 22.502775268040754 27.82020395133669 27.00138020139149 22.675640579231068 34-35 22.91159479635871 27.876574164487984 26.635713610734875 22.57611742841843 36-37 23.035670953040558 27.599725536334308 26.637439595692747 22.72716391493239 38-39 22.54997604557113 27.816623743589574 26.889923791901666 22.743476418937632 40-41 22.813686584275043 27.54246192221928 26.991193922672814 22.65265757083286 42-43 22.72464556831266 27.535468363806775 26.85832558584843 22.881560482032135 44-45 22.635736998723385 27.838036467291882 26.743604518011804 22.78262201597293 46-47 22.729257427376524 27.90708690024865 26.507817210334395 22.855838462040428 48-49 22.648925963327347 27.834702964409747 26.676613876912597 22.83975719535031 50-51 22.59904656493031 27.79474111599925 26.637703995724888 22.968508323345553 52-53 22.894194749948856 27.6823191975466 26.602860952354078 22.820625100150465 54-55 22.71369313633623 27.533821450357117 26.753168481504876 22.999316931801776 56-57 22.582676236893345 27.94499135293335 26.704696627039315 22.76763578313399 58-59 22.697295483132297 27.54252705121589 26.68296559536864 23.077211870283172 60-61 22.758121582907663 27.574941369077095 26.943816368478622 22.723120679536617 62-63 22.518276582464914 27.639423746844923 26.930509300124 22.911790370566166 64-65 22.433757439580106 27.61179589485847 26.754597031877758 23.199849633683666 66-67 22.549387909155524 27.921027721024544 26.437406287880105 23.092178081939824 68-69 22.444164867375733 27.638620573574222 26.873809589202292 23.043404969847757 70-71 22.386717201862545 27.526295779044656 27.0073215229191 23.079665496173703 72-73 22.579556065288607 27.491369279927596 26.925982158559343 23.003092496224454 74-75 22.39262938089057 27.73546153039917 26.740539816016707 23.131369272693558 76-77 22.457081482888654 27.549910042374943 26.871442352921616 23.121566121814794 78-79 22.618720378012526 27.614954916503503 26.745484784587635 23.020839920896332 80-81 22.4100342128118 27.849289276013163 26.76697116967806 22.973705341496974 82-83 22.5825981838402 27.704474186918027 26.785610806055075 22.9273168231867 84-85 22.600102452742853 27.48679087822864 26.715749576872188 23.197357092156317 86-87 22.533915263367685 27.29568324623028 26.956190464672243 23.214211025729796 88-89 22.581983755175713 27.29282154931018 26.99955001463838 23.125644680875723 90-91 22.486513206626846 27.560248400884035 27.048087863448046 22.905150529041073 92-93 22.537740022917635 27.27490781043871 26.97221161220621 23.215140554437443 94-95 22.451070747056384 27.280127625974465 26.973156117586417 23.295645509382737 96-97 22.571558650416463 27.289729421667886 26.893888335575284 23.24482359234037 98-99 22.401729601354003 27.23620630823657 26.89022051459622 23.4718435758132 100-101 22.201985036129592 27.278018955310984 27.120553600712817 23.399442407846607 102-103 22.420595226516955 27.1276357184287 27.08686815952865 23.364900895525697 104-105 22.32528792574028 27.213940176759415 27.089461713801334 23.371310183698967 106-107 22.253636136810986 27.431051386029072 27.020573061714142 23.2947394154458 108-109 22.345341493470354 27.457898626145145 26.693786499593987 23.50297338079052 110-111 22.160356964691534 27.629314037218254 26.947458062695578 23.26287093539463 112-113 22.12357469541005 27.564641106729326 27.032518993561716 23.27926520429891 114-115 21.92854320151297 27.723785127566142 26.872210057704173 23.475461613216716 116-117 22.06240966272909 27.651586381714704 27.014114670798 23.271889284758203 118-119 21.98051240402475 27.6329316907573 26.736303030701457 23.65025287451649 120-121 21.996995766073347 27.716668705644352 26.92618438328072 23.360151145001584 122-123 22.108887936899933 27.673966181992288 26.951717360078625 23.265428521029154 124-125 22.129357232399208 27.681618747219865 26.82002911561325 23.368994904767682 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2648.0 1 2088.5 2 1350.5 3 1042.0 4 772.0 5 589.5 6 524.5 7 491.5 8 470.0 9 463.5 10 456.0 11 436.0 12 429.5 13 472.5 14 531.5 15 589.0 16 678.5 17 782.5 18 976.5 19 1266.5 20 1629.5 21 2175.0 22 2947.0 23 3908.0 24 5090.5 25 6820.0 26 9533.0 27 12697.0 28 15303.0 29 18172.0 30 22067.0 31 26267.5 32 30502.0 33 34938.5 34 39920.5 35 44243.0 36 49108.0 37 53645.5 38 55494.5 39 56728.0 40 58642.0 41 61909.5 42 64999.0 43 68156.5 44 70880.0 45 72532.5 46 72560.5 47 72172.0 48 74287.0 49 75599.0 50 74388.0 51 72482.5 52 70950.0 53 69715.0 54 67241.0 55 62710.5 56 56999.0 57 51515.0 58 47228.0 59 42285.5 60 35940.5 61 29303.0 62 23901.0 63 19006.5 64 14749.5 65 11800.0 66 9057.5 67 7531.0 68 5695.5 69 3404.5 70 2359.5 71 1895.0 72 1571.0 73 1250.5 74 921.5 75 734.5 76 531.5 77 277.5 78 138.0 79 77.0 80 46.0 81 33.5 82 23.5 83 19.5 84 20.0 85 18.0 86 11.0 87 7.0 88 8.5 89 8.0 90 6.5 91 6.0 92 4.5 93 3.5 94 3.0 95 2.0 96 2.5 97 3.0 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06313777084869157 2 0.04948227531796898 3 0.04529996487867016 4 0.04449373636025112 5 0.04388906497143683 6 0.00508931752252023 7 4.031142592095232E-4 8 0.0 9 0.0015620677544369022 10-11 0.003451665844481542 12-13 0.002796605173266067 14-15 0.007986701260588678 16-17 0.0035524444092839232 18-19 0.005164901446122016 20-21 0.00954876901502558 22-23 0.01652768462759045 24-25 0.0042326997216999935 26-27 0.016351322139186282 28-29 0.007684365566181535 30-31 0.006878137047762489 32-33 0.0028217998144666624 34-35 0.018215725588030328 36-37 0.01589781859757557 38-39 0.02889825345708269 40-41 0.006248271017747609 42-43 0.0017132356016404736 44-45 1.763624884041664E-4 46-47 0.0024942694788589246 48-49 0.003678417615286899 50-51 0.0020407659372482113 52-53 0.0017636248840416638 54-55 0.006071908529343443 56-57 0.0030737462264726137 58-59 0.008314231596196415 60-61 0.01584742931517438 62-63 0.012471347394294624 64-65 0.0031241355088738043 66-67 0.004056337233295827 68-69 0.010203829686241055 70-71 0.002897383738068448 72-73 0.003426471203280947 74-75 0.0010581749304249984 76-77 1.763624884041664E-4 78-79 0.003250108714876781 80-81 0.002947773020469638 82-83 0.0016124570368380928 84-85 0.011060447487061292 86-87 0.005945935323340467 88-89 0.002015571296047616 90-91 7.55839236017856E-4 92-93 0.00256985340246071 94-95 0.0 96-97 0.0020407659372482113 98-99 0.025597755459804722 100-101 0.01526795256756069 102-103 0.006449828147352371 104-105 0.013605106248321407 106-107 0.03245069786636662 108-109 0.0011841481364279743 110-111 0.0019651820136464253 112-113 0.01632612749798569 114-115 0.032097972889558284 116-117 0.05089317522520231 118-119 0.35705845509483514 120-121 0.11823845115439327 122-123 0.02010532367807497 124-125 0.313874840077015 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1984549.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.56857830617137 #Duplication Level Percentage of deduplicated Percentage of total 1 86.2397120517807 62.582932971313184 2 8.764285991331318 12.720235485192152 3 2.4240778405877323 5.277356477848369 4 0.9464471721207908 2.7472930289080826 5 0.45387775780025935 1.6468631804178802 6 0.2519080134625665 1.0968363840546198 7 0.16198337498016244 0.8228432262082086 8 0.1082222154799114 0.628282585481704 9 0.08585260144418312 0.5607181107621652 >10 0.49592315993089814 6.788422268491856 >50 0.046556547785850534 2.3194748543446173 >100 0.021007388694894862 2.434715503912427 >500 0.0 0.0 >1k 1.4588460072617035E-4 0.37402592306473964 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3533 0.17802533472340568 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.511678472035712E-4 0.0 22-23 0.0 0.0 0.0 5.542821064130944E-4 0.0 24-25 0.0 0.0 0.0 0.001284926701230355 0.0 26-27 0.0 0.0 0.0 0.0020659605784488066 0.0 28-29 0.0 0.0 0.0 0.004358672927702969 0.0 30-31 0.0 0.0 0.0 0.01221940098228867 5.03892824011904E-5 32-33 0.0 0.0 0.0 0.02839436063307079 5.03892824011904E-5 34-35 0.0 0.0 0.0 0.054949512458498134 5.03892824011904E-5 36-37 0.0 0.0 0.0 0.09289264210659451 5.03892824011904E-5 38-39 0.0 0.0 0.0 0.14748942958828432 5.03892824011904E-5 40-41 0.0 0.0 0.0 0.19956675294991455 5.03892824011904E-5 42-43 0.0 0.0 0.0 0.25194641200595197 5.03892824011904E-5 44-45 0.0 0.0 0.0 0.32276854842082503 5.03892824011904E-5 46-47 0.0 0.0 0.0 0.40659111969520534 5.03892824011904E-5 48-49 0.0 0.0 0.0 0.49406691394367186 5.03892824011904E-5 50-51 0.0 0.0 0.0 0.5886979862931074 5.03892824011904E-5 52-53 0.0 0.0 0.0 0.6838329514665549 5.03892824011904E-5 54-55 0.0 0.0 0.0 0.7892725248910457 5.03892824011904E-5 56-57 0.0 0.0 0.0 0.9181431146320902 5.03892824011904E-5 58-59 0.0 0.0 0.0 1.061676985551881 5.03892824011904E-5 60-61 0.0 0.0 0.0 1.2133487255794642 5.03892824011904E-5 62-63 0.0 0.0 0.0 1.3699838099235646 5.03892824011904E-5 64-65 0.0 0.0 0.0 1.5371754489307143 5.03892824011904E-5 66-67 0.0 0.0 0.0 1.7353313019733956 5.03892824011904E-5 68-69 0.0 0.0 0.0 1.9558096071198041 5.03892824011904E-5 70-71 0.0 0.0 0.0 2.2055388907001037 5.03892824011904E-5 72-73 0.0 0.0 0.0 2.458316725865675 5.03892824011904E-5 74-75 0.0 0.0 0.0 2.7165617981717762 5.03892824011904E-5 76-77 0.0 0.0 0.0 3.0008329348380918 5.03892824011904E-5 78-79 0.0 0.0 0.0 3.324911604601348 5.03892824011904E-5 80-81 0.0 0.0 0.0 3.6912164930168014 5.03892824011904E-5 82-83 0.0 0.0 0.0 4.076719698027109 5.03892824011904E-5 84-85 0.0 0.0 0.0 4.456201383790473 5.03892824011904E-5 86-87 0.0 0.0 0.0 4.8508754381977965 5.03892824011904E-5 88-89 0.0 0.0 0.0 5.289791282553367 5.03892824011904E-5 90-91 0.0 0.0 0.0 5.770631009866726 5.03892824011904E-5 92-93 0.0 0.0 0.0 6.286340120601708 5.03892824011904E-5 94-95 0.0 0.0 0.0 6.80408999727394 5.03892824011904E-5 96-97 0.0 0.0 0.0 7.31627185824084 5.03892824011904E-5 98-99 0.0 0.0 0.0 7.860098188555687 5.03892824011904E-5 100-101 0.0 0.0 0.0 8.475855219498232 5.03892824011904E-5 102-103 0.0 0.0 0.0 9.126254882091597 5.03892824011904E-5 104-105 0.0 0.0 0.0 9.769146541607185 5.03892824011904E-5 106-107 0.0 0.0 0.0 10.414507275960432 5.03892824011904E-5 108-109 0.0 0.0 0.0 11.08062839466297 5.03892824011904E-5 110-111 0.0 0.0 0.0 11.801774609747605 5.03892824011904E-5 112-113 0.0 0.0 0.0 12.559604222420308 5.03892824011904E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2095 0.0 50.55174 1 GTATCAA 3390 0.0 45.807957 1 ATCAACG 3705 0.0 39.017864 3 GTACATG 9210 0.0 38.1147 1 TCAACGC 3835 0.0 37.69427 4 CAACGCA 3855 0.0 37.499657 5 AACGCAG 4040 0.0 36.359566 6 TACATGG 9615 0.0 35.64023 2 ACATGGG 9220 0.0 35.61669 3 TATCAAC 4360 0.0 33.976616 2 CATGGGG 4340 0.0 30.977985 4 ACGCAGA 4855 0.0 29.520245 7 CGCAGAG 4990 0.0 28.721601 8 GGTCGAC 105 5.925087E-4 28.318672 7 CATGGGA 5785 0.0 28.176151 4 GAGTACA 6750 0.0 24.416092 1 AGTACAT 6410 0.0 24.131405 2 ATGGGAG 2955 0.0 23.554491 5 GCAGAGT 6115 0.0 22.369505 9 ATGGGGA 2830 0.0 21.441696 5 >>END_MODULE