FastQCFastQC Report
Thu 26 May 2016
SRR1364210_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364210_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1984549
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82340.41490535129140177No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76330.3846213925682863No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44150.22246868180125562No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21590.10879046070417007No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44750.074.230251
GTATCAA78350.050.222951
ATCAACG98150.039.890313
TCAACGC99600.039.249834
CAACGCA101450.038.4705925
TATCAAC103300.037.7316322
AACGCAG105050.037.4901436
GCGCGAC650.001940593736.6101769
GTGGTAT21650.034.6459431
TGGTATC21250.033.6036872
GTACATG93400.032.1235431
ACGCAGA123000.031.7771877
CGCAGAG123550.031.5875748
TACATGG95900.031.087352
CGTATAG1352.322362E-630.8676851
ACATGGG93850.030.622783
GCAGAGT139350.027.8779859
GAGTACT89050.027.79306612-13
CAGAGTA134300.026.20196710-11
GTACTTT97800.025.30646714-15
Produced by FastQC (version 0.11.5)