FastQCFastQC Report
Thu 26 May 2016
SRR1364209_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364209_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2783847
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35150.046.2219621
GGTATCA21450.044.39191
ATCAACG36950.042.186453
CAACGCA39250.039.5620885
TCAACGC40450.038.389124
AACGCAG43400.035.8993846
TATCAAC45100.035.3570182
GTACATG81450.031.3456481
TACATGG81750.030.5688362
ACGCAGA51000.030.4325267
CGCAGAG51650.029.8182038
ACATGGG79900.029.7851183
CATGGGG53450.026.2694154
GCAGAGT74500.020.4350059
GAGTACA62150.020.108941
AGAGTAC56050.019.31020410-11
GTATAGG8100.019.1029011
GTGTAGC24900.018.88161
TATACCG1600.00683855418.5920965
CAGAGTA60900.018.30943910-11
Produced by FastQC (version 0.11.5)