FastQCFastQC Report
Thu 26 May 2016
SRR1364209_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364209_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2783847
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46950.16865150994289557No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40780.146487935579793No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39250.061.2929341
GTATCAA70150.050.8472981
ATCAACG79550.044.209893
TCAACGC81300.043.1118664
CAACGCA81350.043.0044945
TATCAAC85550.040.902782
AACGCAG86600.040.5998736
ACGCAGA100450.034.7052357
CGCAGAG101600.034.1367458
TGGTATC19700.034.1356162
GTGGTAT22350.032.5050661
GTACATG78650.027.7866421
GCAGAGT125750.027.2970129
TACATGG78350.026.8121242
GAGTACT80100.025.6236912-13
ACATGGG79550.025.5833853
CAGAGTA116200.024.57437110-11
GTACTTT85000.024.0765714-15
AGAGTAC110300.023.19210610-11
CATGGGG54900.022.1120974
Produced by FastQC (version 0.11.5)