FastQCFastQC Report
Thu 26 May 2016
SRR1364208_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364208_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2917259
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22350.059.0960921
GTATCAA41100.044.1510851
ATCAACG44450.039.212733
TCAACGC44350.038.899414
CAACGCA46000.037.6340755
AACGCAG48100.035.9774366
TATCAAC51200.034.509022
TACATGG109100.032.880522
GTACATG111150.032.75861
ACATGGG108000.032.057563
CATGGGG80650.028.914814
ACGCAGA59550.028.659437
CGCAGAG60550.027.5968558
GTATAGG10950.024.99351
TAAGGTG11700.024.4062735
CGTATAG2052.2666263E-623.2177141
GAGTACA80000.022.682621
ATGGGGG49850.021.2422735
AGTACAT80800.020.7627012
GTATAAT19550.020.3897251
Produced by FastQC (version 0.11.5)