FastQCFastQC Report
Thu 26 May 2016
SRR1364208_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364208_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2917259
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63230.21674455370606452No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60160.2062209766085219No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32910.11281137533554614No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42000.074.9799351
GTATCAA81200.051.392611
ATCAACG101200.040.752093
TCAACGC101700.040.4911354
CAACGCA103550.039.756835
AACGCAG108100.038.688816
TATCAAC107400.038.5684282
TGGTATC22550.035.1020132
ACGCAGA126250.032.9848867
CGCAGAG128700.032.218298
GTGGTAT25750.031.6724741
TACATGG108050.030.2394942
ACATGGG104200.030.0410583
GTACATG112700.029.7387771
CATGGGG75300.026.9484774
GCAGAGT154500.026.2990769
GAGTACT97700.025.36244212-13
AGAGTAC134250.024.81544310-11
TAAGGTG12350.024.5673855
CAGAGTA148000.023.91682410-11
Produced by FastQC (version 0.11.5)