FastQCFastQC Report
Thu 26 May 2016
SRR1364207_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364207_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2307195
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19200.050.8128971
GTATCAA31850.038.2891541
TCAACGC32000.035.1322144
CAACGCA32200.034.9145
ATCAACG32450.034.6450163
GTACATG70000.033.9083181
AACGCAG33900.032.8051076
ACATGGG73050.030.3726233
TACATGG76100.029.9369682
TATCAAC43050.027.2199132
ACGCAGA41350.026.7490737
CGCAGAG42250.025.4755278
CATGGGG46200.024.205254
GAGTACA53450.021.5916271
AGTACAT55050.019.9897842
CAGAGTA48250.019.3513510-11
AGAGTAC46100.019.2218110-11
GCAGAGT55200.019.1774069
CGCGGAA2650.019.07783582-83
CATGGGA37950.018.4954074
Produced by FastQC (version 0.11.5)