##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364207_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2307195 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.12948363705712 33.0 31.0 34.0 28.0 34.0 2 31.17578401478852 33.0 31.0 34.0 28.0 34.0 3 31.25454198713156 34.0 31.0 34.0 28.0 34.0 4 34.65904832491402 37.0 35.0 37.0 32.0 37.0 5 34.72810967430148 37.0 35.0 37.0 32.0 37.0 6 34.81383628171871 37.0 35.0 37.0 32.0 37.0 7 34.71435877764992 37.0 35.0 37.0 32.0 37.0 8 34.688935698976465 37.0 35.0 37.0 32.0 37.0 9 36.21873313699102 39.0 37.0 39.0 32.0 39.0 10-11 36.16607092161694 39.0 37.0 39.0 31.5 39.0 12-13 36.121626693885865 39.0 37.0 39.0 31.0 39.0 14-15 37.44805987356942 40.0 38.0 41.0 31.5 41.0 16-17 37.477825454718825 40.0 38.0 41.0 31.5 41.0 18-19 37.37312407490481 40.0 38.0 41.0 31.0 41.0 20-21 37.345147679324896 40.0 38.0 41.0 31.0 41.0 22-23 37.3769705204805 40.0 38.0 41.0 31.0 41.0 24-25 37.2217513040727 40.0 38.0 41.0 30.0 41.0 26-27 37.23959981709392 40.0 37.5 41.0 30.0 41.0 28-29 37.329406920524704 40.0 38.0 41.0 30.0 41.0 30-31 37.226234020098 40.0 37.0 41.0 30.0 41.0 32-33 37.038412877975205 40.0 37.0 41.0 30.0 41.0 34-35 37.000110957244615 40.0 37.0 41.0 30.0 41.0 36-37 37.03480503381812 40.0 37.0 41.0 30.0 41.0 38-39 36.88353975281673 40.0 37.0 41.0 30.0 41.0 40-41 36.89504073127759 40.0 37.0 41.0 30.0 41.0 42-43 36.839804394513685 40.0 37.0 41.0 30.0 41.0 44-45 36.84496954093607 40.0 37.0 41.0 30.0 41.0 46-47 36.78429651589917 40.0 37.0 41.0 30.0 41.0 48-49 36.71167109845505 40.0 36.0 41.0 30.0 41.0 50-51 36.60995949627144 40.0 36.0 41.0 30.0 41.0 52-53 36.479541607883164 40.0 36.0 41.0 30.0 41.0 54-55 36.31660587856683 40.0 35.5 41.0 29.5 41.0 56-57 36.14258070947622 39.0 35.0 41.0 29.0 41.0 58-59 35.93407882732062 39.0 35.0 41.0 29.0 41.0 60-61 35.705216724204064 39.0 35.0 41.0 29.0 41.0 62-63 35.42860963204237 38.0 35.0 40.0 28.0 41.0 64-65 35.1341215198542 38.0 34.0 40.0 28.0 41.0 66-67 34.834640981798245 37.0 34.0 40.0 28.0 41.0 68-69 34.60607339214934 37.0 34.0 39.5 28.0 41.0 70-71 34.428813776035405 36.5 34.0 39.0 28.0 41.0 72-73 34.105279137654165 36.0 34.0 39.0 28.0 41.0 74-75 33.757055645491604 35.5 34.0 38.5 28.0 40.0 76-77 33.41139847303761 35.0 34.0 37.0 27.5 39.5 78-79 33.05233584504127 35.0 34.0 37.0 27.0 39.0 80-81 32.73762555830781 35.0 33.0 36.5 26.0 39.0 82-83 32.45825688769263 35.0 33.0 36.0 26.0 37.0 84-85 32.20021714679513 35.0 33.0 36.0 26.0 37.0 86-87 31.981570478438105 35.0 33.0 35.0 26.0 37.0 88-89 31.804672340222652 35.0 33.0 35.0 25.5 36.0 90-91 31.6460041305568 35.0 33.0 35.0 25.0 36.0 92-93 31.514341223867078 35.0 33.0 35.0 25.0 36.0 94-95 31.38214151816383 35.0 33.0 35.0 25.0 36.0 96-97 31.284464468759683 35.0 33.0 35.0 24.0 35.0 98-99 31.20680913403505 35.0 33.0 35.0 24.0 35.0 100-101 31.106410814863935 35.0 32.5 35.0 23.5 35.0 102-103 30.133245130992396 34.0 30.5 35.0 20.0 35.0 104-105 30.813405672255705 35.0 32.0 35.0 22.0 35.0 106-107 30.911657228799474 35.0 32.0 35.0 23.0 35.0 108-109 30.878381324508766 35.0 32.0 35.0 20.5 35.0 110-111 30.809223537672366 35.0 32.0 35.0 20.0 35.0 112-113 30.94538389689645 35.0 33.0 35.0 21.5 35.0 114-115 30.93361766127267 35.0 33.0 35.0 20.0 35.0 116-117 30.83814306983155 35.0 33.0 35.0 19.5 35.0 118-119 30.593804381510882 35.0 33.0 35.0 13.0 35.0 120-121 30.52866814465184 35.0 33.0 35.0 6.0 35.0 122-123 30.396138817915258 35.0 33.0 35.0 2.0 35.0 124-125 29.545294177561928 34.5 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5620374344044059 1101 2 0.6235125688133785 1101 3 0.5865969331801004 1101 4 0.590254422437404 1101 5 0.5457158453551827 1101 6 0.5523942022584905 1101 7 0.5930617528771265 1101 8 0.5137583292838812 1101 9 0.6419444969018784 1101 10-11 0.6202469292722554 1101 12-13 0.6177513629860343 1101 14-15 0.7199304220936682 1101 16-17 0.6926331892348045 1101 18-19 0.81583253626588 1101 20-21 0.915879085973657 1101 22-23 0.9024936658477287 1101 24-25 0.9934234389550696 1101 26-27 0.9305786526781006 1101 28-29 0.8650631214510511 1101 30-31 0.984140844525669 1101 32-33 1.1022305037861813 1101 34-35 1.0348415549540988 1101 36-37 0.9977439816530662 1101 38-39 1.087598111764997 1101 40-41 0.9142616597263782 1101 42-43 0.8331762283232607 1101 44-45 0.7311197014721458 1101 46-47 0.6923610660273667 1101 48-49 0.7100815084174883 1101 50-51 0.6760426139847766 1101 52-53 0.6788344206649626 1101 54-55 0.7582422242131912 1101 56-57 0.6681600496505595 1101 58-59 0.7642324229199815 1101 60-61 0.6130491515940903 1101 62-63 0.6262889022731812 1101 64-65 0.6159253967476701 1101 66-67 0.557706882170784 1101 68-69 0.5300694514893607 1101 70-71 0.6315578930412826 1101 72-73 0.6135189613395866 1101 74-75 0.6265334478169322 1101 76-77 0.60651245213122 1101 78-79 0.639361589515488 1101 80-81 0.5704162723672468 1101 82-83 0.5752066459489455 1101 84-85 0.5883913284285711 1101 86-87 0.549038009243624 1101 88-89 0.5184228482696476 1101 90-91 0.5434623158431577 1101 92-93 0.5747916198249605 1101 94-95 0.6341907932944224 1101 96-97 0.6123043034730564 1101 98-99 0.5482885410518463 1101 100-101 0.5721596381382845 1101 102-103 0.4686006307483588 1101 104-105 0.5891711384896503 1101 106-107 0.5298548650787858 1101 108-109 0.47926045075208634 1101 110-111 0.4747173361185233 1101 112-113 0.5435833659468123 1101 114-115 0.5852618061653203 1101 116-117 0.5764475058468363 1101 118-119 0.41232044017501224 1101 120-121 0.3173947432356634 1101 122-123 0.41640902758603815 1101 124-125 0.31680420572444845 1102 1 0.05659207668372446 1102 2 0.1073805427701835 1102 3 0.03796104899665664 1102 4 0.10146498680647653 1102 5 -0.0065192493668249085 1102 6 0.09327952160283104 1102 7 0.057198167684454404 1102 8 0.10664018148612087 1102 9 0.18404110408859964 1102 10-11 0.1355315699341304 1102 12-13 0.14505068324369574 1102 14-15 0.18930524439341667 1102 16-17 0.18825447077789903 1102 18-19 0.26728282374283907 1102 20-21 0.39914892722887174 1102 22-23 0.4045726147562263 1102 24-25 0.4990108647126519 1102 26-27 0.47460485992233004 1102 28-29 0.3004275100677347 1102 30-31 0.32480202539076686 1102 32-33 0.4843473037117221 1102 34-35 0.4183937351201479 1102 36-37 0.3231840676957063 1102 38-39 0.43157994290816504 1102 40-41 0.3152561820658022 1102 42-43 0.30830335456290214 1102 44-45 0.23423289462984087 1102 46-47 0.27111491322336434 1102 48-49 0.12983555514464484 1102 50-51 0.07357345030608542 1102 52-53 0.05458769691609433 1102 54-55 0.02615323423260918 1102 56-57 0.014837671331171975 1102 58-59 0.04496941641378527 1102 60-61 -0.018280329666332307 1102 62-63 0.10998656047766531 1102 64-65 0.08797541133233722 1102 66-67 0.042113359883430235 1102 68-69 0.08489084805087543 1102 70-71 0.032709183289085786 1102 72-73 0.024285349968245384 1102 74-75 0.010641135665387935 1102 76-77 0.0010090783033547268 1102 78-79 0.08974468475544484 1102 80-81 0.10054299078912976 1102 82-83 0.057859599494129554 1102 84-85 0.02734027379344184 1102 86-87 0.045042040418408646 1102 88-89 0.023510985289043163 1102 90-91 -0.023449471564440216 1102 92-93 0.07713355358153251 1102 94-95 0.0966690257339522 1102 96-97 0.024624777260516595 1102 98-99 -0.016543329744116875 1102 100-101 -0.02464555907269883 1102 102-103 -0.04196903765663862 1102 104-105 -0.02248779500011011 1102 106-107 0.007245402877501306 1102 108-109 0.01769692818363211 1102 110-111 0.002878649466790506 1102 112-113 0.017145992698644363 1102 114-115 0.043327911349120285 1102 116-117 -0.020812051028073597 1102 118-119 -0.048275333174167656 1102 120-121 -0.02762156256221715 1102 122-123 -0.04983232587122188 1102 124-125 -0.06433618173467437 1103 1 0.3141957570461713 1103 2 0.08158578705069885 1103 3 0.316334200284313 1103 4 0.37275730661828277 1103 5 0.34485662098916237 1103 6 0.3948094966992173 1103 7 0.4444080187709929 1103 8 0.46663375591744227 1103 9 0.6034612963474189 1103 10-11 0.536590745065368 1103 12-13 0.524153018593033 1103 14-15 0.6761343714599448 1103 16-17 0.5226538616194603 1103 18-19 0.6189072311021064 1103 20-21 0.7219616926919841 1103 22-23 0.6771433323143654 1103 24-25 0.9134202932363635 1103 26-27 0.8749392980662449 1103 28-29 0.7862366688627773 1103 30-31 0.8500810566183077 1103 32-33 0.9497660366040961 1103 34-35 0.9557485699068025 1103 36-37 0.821021382824469 1103 38-39 0.9388132455052656 1103 40-41 0.8555183144107374 1103 42-43 0.8205772344446984 1103 44-45 0.7816066249447218 1103 46-47 0.8143243214175939 1103 48-49 0.7581629650959272 1103 50-51 0.789315549034761 1103 52-53 0.8386668530247405 1103 54-55 0.7679769249776172 1103 56-57 0.7986087540939408 1103 58-59 0.7673646106692615 1103 60-61 0.7840625070951788 1103 62-63 0.7514343826818148 1103 64-65 0.7736174871903572 1103 66-67 0.7933657558581899 1103 68-69 0.8237987554936481 1103 70-71 0.8390143179356073 1103 72-73 0.8354444662891893 1103 74-75 0.8344994173884572 1103 76-77 0.835158616170645 1103 78-79 0.8210492748622471 1103 80-81 0.8004767384566875 1103 82-83 0.6915217743131592 1103 84-85 0.7759175730216441 1103 86-87 0.8239329582219526 1103 88-89 0.756242224080605 1103 90-91 0.6902638536855079 1103 92-93 0.6714648043529614 1103 94-95 0.691181608231723 1103 96-97 0.7093279305085147 1103 98-99 0.6724404956111023 1103 100-101 0.671871156481533 1103 102-103 0.5900599717893087 1103 104-105 0.6541045298940666 1103 106-107 0.7465254578397094 1103 108-109 0.7054204163598605 1103 110-111 0.6892609816941011 1103 112-113 0.7221616572912204 1103 114-115 0.7340684218369553 1103 116-117 0.7704543486931215 1103 118-119 0.6583855220436163 1103 120-121 0.6785991230495902 1103 122-123 0.8033665273281301 1103 124-125 0.7124775893117885 1104 1 0.337034365805831 1104 2 0.3808947675438894 1104 3 0.31960711158354016 1104 4 0.3225263533610203 1104 5 0.25210358455191084 1104 6 0.32263614373638916 1104 7 0.32325969341214034 1104 8 0.40325318911582286 1104 9 0.5010698719823097 1104 10-11 0.465748363762458 1104 12-13 0.4597451238561945 1104 14-15 0.5054396714231402 1104 16-17 0.5009322937136886 1104 18-19 0.6536077279736574 1104 20-21 0.6109145819080339 1104 22-23 0.6634361184755733 1104 24-25 0.852973108841816 1104 26-27 0.7869019406867821 1104 28-29 0.632508752042753 1104 30-31 0.666792963132842 1104 32-33 0.7604276118709308 1104 34-35 0.7665688680297293 1104 36-37 0.7361598627655752 1104 38-39 0.7561963886778784 1104 40-41 0.7773525985476581 1104 42-43 0.6237181067303155 1104 44-45 0.5689865476536013 1104 46-47 0.5324012444945154 1104 48-49 0.47683153336975437 1104 50-51 0.5076059428514768 1104 52-53 0.5208185828738365 1104 54-55 0.5326801822466578 1104 56-57 0.5279333768396199 1104 58-59 0.5242333997748929 1104 60-61 0.5401763274853195 1104 62-63 0.5492297452140136 1104 64-65 0.5925901592551384 1104 66-67 0.5569593333333529 1104 68-69 0.48943916771300877 1104 70-71 0.4448120728123115 1104 72-73 0.3767386326491433 1104 74-75 0.4408023250071551 1104 76-77 0.3970467280587542 1104 78-79 0.4493571511964376 1104 80-81 0.4275415157846254 1104 82-83 0.37496859065471 1104 84-85 0.5335620338239977 1104 86-87 0.4617632857891252 1104 88-89 0.48553418212256005 1104 90-91 0.4546841702110811 1104 92-93 0.4894911201424357 1104 94-95 0.5228829332225189 1104 96-97 0.5202848683075558 1104 98-99 0.5025366494572552 1104 100-101 0.577555274994598 1104 102-103 0.5656313740712378 1104 104-105 0.626742549768931 1104 106-107 0.628388266084098 1104 108-109 0.5790912849649388 1104 110-111 0.585795762702567 1104 112-113 0.6186403102172413 1104 114-115 0.5892400265480333 1104 116-117 0.5797191628263576 1104 118-119 0.4763782529968701 1104 120-121 0.49603793423840514 1104 122-123 0.5911218640966283 1104 124-125 0.6575263563342375 1105 1 0.1930698048020325 1105 2 0.21009003125758596 1105 3 0.20154611194108796 1105 4 0.27636552251528457 1105 5 0.20867077479329765 1105 6 0.12261061635057757 1105 7 0.1510869553297738 1105 8 0.09933278134285928 1105 9 0.14592647792096614 1105 10-11 0.23064604634146946 1105 12-13 0.2437976587172912 1105 14-15 0.2191713262130648 1105 16-17 0.20327836025118984 1105 18-19 0.33464013195493436 1105 20-21 0.47957751231888324 1105 22-23 0.4177819688202362 1105 24-25 0.6608060666093749 1105 26-27 0.6204138271356499 1105 28-29 0.5407946753940749 1105 30-31 0.5000415516430223 1105 32-33 0.6800864429328541 1105 34-35 0.6962735984954591 1105 36-37 0.6243166524681527 1105 38-39 0.6693950033806502 1105 40-41 0.5695326955018274 1105 42-43 0.5508658163334346 1105 44-45 0.4341244537427471 1105 46-47 0.3577250348539778 1105 48-49 0.36203758178032786 1105 50-51 0.2928457506061477 1105 52-53 0.23889687794380876 1105 54-55 0.304978436905202 1105 56-57 0.2658854069735739 1105 58-59 0.3146955535545217 1105 60-61 0.3519202138011437 1105 62-63 0.35453632329706153 1105 64-65 0.3767370290321992 1105 66-67 0.36693301601214756 1105 68-69 0.3637780749413295 1105 70-71 0.3756629163801648 1105 72-73 0.38473519019385805 1105 74-75 0.35922860740489426 1105 76-77 0.3377123941405955 1105 78-79 0.3121281198235266 1105 80-81 0.2651242303352319 1105 82-83 0.24862299807843868 1105 84-85 0.20550057685053247 1105 86-87 0.14671385246382584 1105 88-89 0.13418447356714225 1105 90-91 0.13491919677785802 1105 92-93 0.12560667521756486 1105 94-95 0.19371369580120046 1105 96-97 0.1318960454775926 1105 98-99 0.07993360446420184 1105 100-101 0.10224408202585167 1105 102-103 0.06235492994461467 1105 104-105 0.11606632349817048 1105 106-107 0.2193806328136816 1105 108-109 0.14506332125207777 1105 110-111 0.1360874983153515 1105 112-113 0.08477074933816553 1105 114-115 0.1500321654436192 1105 116-117 0.19968070544545213 1105 118-119 0.0434770697568041 1105 120-121 0.04675864634436877 1105 122-123 -0.09538250549335459 1105 124-125 0.013461764564532075 1106 1 0.2912033739870594 1106 2 0.2635585803095353 1106 3 0.2801153609716245 1106 4 0.26368889753781843 1106 5 0.19446362776735526 1106 6 0.1670678016914664 1106 7 0.06315865392210185 1106 8 0.11931277108171656 1106 9 0.13830733919941451 1106 10-11 0.26357778485053274 1106 12-13 0.23791160096360642 1106 14-15 0.23360014638605975 1106 16-17 0.3244902835853196 1106 18-19 0.3398552889666391 1106 20-21 0.3787644916723991 1106 22-23 0.3654584138990913 1106 24-25 0.5802901502525444 1106 26-27 0.5156171765710624 1106 28-29 0.46961579978116674 1106 30-31 0.490820666590281 1106 32-33 0.6758090939326635 1106 34-35 0.6878584948997997 1106 36-37 0.530303193976124 1106 38-39 0.49500271473471713 1106 40-41 0.3860220553637461 1106 42-43 0.3922857508987718 1106 44-45 0.29311386636339876 1106 46-47 0.27093619223516896 1106 48-49 0.15769572009952526 1106 50-51 0.22993135674511933 1106 52-53 0.18398637231454273 1106 54-55 0.19938508630390572 1106 56-57 0.14715305736276463 1106 58-59 0.2350047058618756 1106 60-61 0.24700139650997954 1106 62-63 0.17689478892794597 1106 64-65 0.1225141253094435 1106 66-67 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-2.0584017181789704 2216 72-73 -1.9644862788601856 2216 74-75 -2.125456812281435 2216 76-77 -2.125913850250427 2216 78-79 -2.1212269840239912 2216 80-81 -2.162540523997894 2216 82-83 -2.171935600045103 2216 84-85 -2.108688084544923 2216 86-87 -2.073459819824116 2216 88-89 -2.008448558016738 2216 90-91 -2.044827498588276 2216 92-93 -2.0873859821159897 2216 94-95 -2.0377538827679373 2216 96-97 -2.0727883760921415 2216 98-99 -2.015301907656408 2216 100-101 -2.12979415885874 2216 102-103 -2.1357788090001577 2216 104-105 -2.092808017961854 2216 106-107 -2.1206310408108706 2216 108-109 -2.09439027500542 2216 110-111 -2.121401630893015 2216 112-113 -2.1367058990730925 2216 114-115 -2.1862061934791086 2216 116-117 -2.169055027996663 2216 118-119 -2.035689850055377 2216 120-121 -1.8942255400170147 2216 122-123 -1.8611364740917367 2216 124-125 -1.7593538789602903 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 35414.0 3 7165.0 4 4801.0 5 4108.0 6 4046.0 7 4619.0 8 4719.0 9 5064.0 10 5892.0 11 6617.0 12 7262.0 13 7507.0 14 7679.0 15 7553.0 16 7037.0 17 6370.0 18 6017.0 19 5768.0 20 5744.0 21 6001.0 22 6948.0 23 9274.0 24 13442.0 25 20179.0 26 24849.0 27 24655.0 28 27422.0 29 34119.0 30 40406.0 31 49126.0 32 63110.0 33 84058.0 34 119358.0 35 182161.0 36 362103.0 37 746883.0 38 348403.0 39 1316.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.297012054567574 18.611480354676214 12.222711334416127 24.868796256340087 2 15.765527457411915 20.210282197564315 39.64980720229102 24.37438314273275 3 19.010480242169955 24.523610573297734 29.378327245984536 27.087581938547768 4 12.529052624275852 16.40649391195754 37.2116852256565 33.8527682381101 5 13.544487892955356 38.12073482619295 34.7282386702154 13.6065386106363 6 32.50282389391348 36.68097323997507 17.31036132838744 13.505841537724015 7 28.246332765253634 32.299058420900245 21.48047753369036 17.974131280155756 8 25.373321284070048 36.60925929537815 20.019807601871538 17.99761181868026 9 25.891871087201128 16.39843063282668 19.727181155111108 37.98251712486109 10-11 24.526398827970926 26.966204472261836 28.301511414713648 20.205885285053597 12-13 24.85968025801625 24.495094904235394 28.08288958436229 22.562335253386063 14-15 22.094939545544783 26.202890647959755 25.20322701174586 26.498942794749603 16-17 22.54845188879363 29.092241716345907 26.074948399182706 22.28435799567775 18-19 22.506303403382706 28.33614423730011 27.244767969305016 21.912784390012167 20-21 23.016637776069917 27.713277840847272 27.19023450148441 22.079849881598403 22-23 22.919062628287353 27.551192479039443 26.982273966082502 22.547470926590698 24-25 22.73675523455877 27.675837267171744 27.21961892732885 22.367788570940643 26-27 22.631651614069455 27.86028427008107 26.96757555902396 22.540488556825515 28-29 22.506523510645675 27.75025573895554 27.163183126430408 22.580037623968373 30-31 22.68907453750932 27.602716286819533 27.180141362560313 22.528067813110834 32-33 22.463921232331206 27.757099213116504 27.203101502965037 22.57587805158726 34-35 22.896600604948613 27.759013268337302 26.95719083625313 22.38719529046095 36-37 22.866123532527794 27.58533468466633 26.851349324492446 22.697192458313427 38-39 22.524191870144282 27.712502601276363 27.16322142064373 22.60008410793563 40-41 22.769374608268684 27.470973955664903 27.19677287082381 22.562878565242606 42-43 22.662989711387908 27.44061157374047 27.115970374865228 22.78042834000639 44-45 22.647361521572872 27.661379647073076 27.07846314447674 22.612795686877313 46-47 22.691765480967298 27.740315692863156 26.790593145865188 22.777325680304358 48-49 22.609851237348195 27.725402734954642 26.89439245227244 22.77035357542472 50-51 22.552762020014285 27.62468619319403 26.949936545143103 22.872615241648578 52-53 22.760845099786124 27.586389237501784 26.961746684613697 22.69101897809839 54-55 22.679398233433346 27.438543367052816 27.076526370632237 22.805532028881608 56-57 22.522413577505827 27.854015762809315 27.020667800971722 22.60290285871314 58-59 22.65594031456482 27.57258958872853 26.891215258068986 22.88025483863767 60-61 22.6456051006218 27.535398828535463 27.23258408847366 22.58641198236908 62-63 22.604052179269303 27.639247199147437 27.114069056666835 22.642631564916428 64-65 22.466085745914206 27.56156751513344 27.06461067415876 22.907736064793585 66-67 22.33445002477122 27.874035645414853 26.828984969526847 22.962529360287075 68-69 22.37420358248899 27.570756725863323 27.0885010933165 22.966538598331187 70-71 22.330434579081768 27.51789498732305 27.138095837683053 23.013574595912125 72-73 22.493918846288548 27.450839311906407 27.172983033451114 22.882258808353935 74-75 22.378257064802572 27.609563548873794 27.048596618291533 22.9635827680321 76-77 22.515968579920052 27.462836779160078 27.063844413131662 22.95735022778821 78-79 22.495722494747255 27.54626694883573 27.13475956573705 22.82325099067997 80-81 22.30382654161405 27.574865022015395 27.234226164869813 22.88708227150074 82-83 22.40138126048905 27.567368306079143 27.18590497094273 22.845345462489078 84-85 22.389645754125457 27.530487130882804 27.149617439770225 22.930249675221514 86-87 22.432734327794073 27.301969704982042 27.296768248227387 22.9685277189965 88-89 22.48110667468806 27.284579683108785 27.19477245657439 23.039541185628764 90-91 22.495882419230405 27.4584341057048 27.262979048015325 22.78270442704947 92-93 22.472363516656223 27.301068140410173 27.319077446735474 22.90749089619813 94-95 22.388744774498903 27.15609213785571 27.35544676544462 23.099716322200766 96-97 22.48549190975402 27.223631120671683 27.19220721917543 23.098669750398866 98-99 22.336225392461223 27.033469590893507 27.18492227509807 23.445382741547196 100-101 22.181245663775815 27.08951007087728 27.425224042313527 23.304020223033373 102-103 22.336916180025856 27.077023437864035 27.41936427678981 23.166696105320305 104-105 22.393656449536394 27.030530016342208 27.40709433831402 23.168719195807377 106-107 22.184008686816966 27.228050840164258 27.355734389602183 23.232206083416596 108-109 22.26087469101743 27.20286203568096 27.025436801552033 23.510826471749578 110-111 22.157943908827953 27.330115950188862 27.283565101513574 23.228375039469615 112-113 22.226516221128758 27.273296726209956 27.418777312831377 23.081409739829905 114-115 22.112098324165736 27.374243060785357 27.261235997681325 23.25242261736758 116-117 22.10362194829245 27.39438667288505 27.206989567295466 23.295001811527037 118-119 22.086079572331613 27.31066311001304 27.120970083439268 23.48228723421608 120-121 22.034444400327793 27.39019130643071 27.286415926429047 23.28894836681245 122-123 22.166448973257307 27.410750398502486 27.240966879533683 23.18183374870652 124-125 22.181227160398816 27.37097588755541 27.105721822163463 23.342075129882314 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1030.0 1 878.5 2 687.0 3 573.5 4 455.5 5 367.5 6 321.0 7 325.5 8 329.0 9 345.0 10 341.0 11 320.5 12 311.5 13 326.5 14 389.0 15 453.5 16 544.5 17 680.0 18 863.0 19 1076.5 20 1434.5 21 2077.0 22 2933.5 23 4057.0 24 5552.5 25 7624.0 26 10736.0 27 14269.5 28 17164.5 29 20344.5 30 25244.0 31 30380.5 32 35773.0 33 40823.0 34 46653.0 35 52024.5 36 56880.5 37 62354.5 38 65584.0 39 68192.5 40 70570.0 41 74855.5 42 79348.0 43 83647.5 44 87389.5 45 88863.5 46 89606.0 47 90465.0 48 91993.5 49 91977.0 50 89911.0 51 86393.0 52 83465.0 53 80969.5 54 76575.5 55 70544.5 56 64167.5 57 57853.5 58 52196.0 59 46010.5 60 38528.0 61 30719.0 62 24082.0 63 18600.0 64 14056.0 65 11093.5 66 8575.0 67 6791.0 68 5229.5 69 3451.0 70 2346.0 71 1759.5 72 1316.0 73 1043.5 74 828.5 75 635.0 76 427.5 77 244.0 78 141.5 79 95.0 80 59.0 81 36.0 82 25.5 83 14.0 84 11.0 85 12.5 86 13.5 87 11.5 88 10.5 89 7.5 90 7.0 91 4.0 92 2.0 93 3.0 94 2.0 95 2.0 96 1.5 97 1.0 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.061980023361701116 2 0.04988741740511747 3 0.045336436668768784 4 0.04485966725829416 5 0.04407949913206296 6 0.004204239346912593 7 3.0339871575657887E-4 8 0.0 9 9.968814946287592E-4 10-11 0.003684127262758458 12-13 0.0023188330418538526 14-15 0.007324911851837404 16-17 0.0026439030944501872 18-19 0.004681008757387218 20-21 0.009362017514774435 22-23 0.015690047871983078 24-25 0.0032940431996428565 26-27 0.014714837714194075 28-29 0.006718114420324247 30-31 0.0064363870414074235 32-33 0.0024055183892128756 34-35 0.016795286050810616 36-37 0.014606481029995297 38-39 0.027522597786489657 40-41 0.005591204904656953 42-43 0.0014086368945841162 44-45 1.5169935787828943E-4 46-47 0.0020587769997767852 48-49 0.0027522597786489656 50-51 0.0018637349682189845 52-53 0.0015386649156226499 54-55 0.005244463515220864 56-57 0.0024055183892128756 58-59 0.0072165551676386264 60-61 0.01469316637735432 62-63 0.011420794514551219 64-65 0.0022971617050140973 66-67 0.0035540992417199243 68-69 0.009687087567370769 70-71 0.002362175715533364 72-73 0.002903959136527255 74-75 7.801681262312027E-4 76-77 3.4674138943609017E-4 78-79 0.0029256304733670108 80-81 0.0025788890839309204 82-83 0.0011702521893468044 84-85 0.010315556335723682 86-87 0.006458058378247179 88-89 0.0013436228840648492 90-91 6.50140105192669E-4 92-93 0.002362175715533364 94-95 0.0 96-97 0.0014736509051033833 98-99 0.023144987744859017 100-101 0.013891326914283362 102-103 0.0059596176309327994 104-105 0.01235266199866071 106-107 0.030838312322972267 108-109 0.0011269095156672929 110-111 0.0017553782840202062 112-113 0.015516677177265032 114-115 0.03042655692301691 116-117 0.04895554992100798 118-119 0.35616842096138385 120-121 0.11696020492416115 122-123 0.01881072037690789 124-125 0.3174634133655803 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2307195.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.93783401089976 #Duplication Level Percentage of deduplicated Percentage of total 1 87.31642885361398 64.55987623003048 2 8.291661940862554 12.261350485159808 3 2.1024261207617077 4.663465006111771 4 0.8213128530695588 2.4290437360510215 5 0.39558829437160065 1.4624470822951179 6 0.23566075326848304 1.0454547394829221 7 0.15228140093402198 0.7881549861644894 8 0.09385353336397106 0.5551461576961388 9 0.08184681711239716 0.5446418740179202 >10 0.44231174290469777 6.376189073838441 >50 0.04227562958106061 2.1827018817385384 >100 0.024225226695951698 2.9945192162986065 >500 0.0 0.0 >1k 1.26833460036069E-4 0.13700953111470612 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.1671336839755635E-5 0.0 12-13 0.0 2.1671336839755635E-5 0.0 6.50140105192669E-5 0.0 14-15 0.0 4.334267367951127E-5 0.0 1.300280210385338E-4 0.0 16-17 0.0 4.334267367951127E-5 0.0 1.300280210385338E-4 0.0 18-19 0.0 4.334267367951127E-5 0.0 1.950420315578007E-4 0.0 20-21 0.0 4.334267367951127E-5 0.0 2.1671336839755633E-4 0.0 22-23 0.0 4.334267367951127E-5 0.0 2.3838470523731198E-4 0.0 24-25 0.0 4.334267367951127E-5 0.0 4.7676941047462395E-4 0.0 26-27 0.0 4.334267367951127E-5 0.0 9.535388209492479E-4 0.0 28-29 0.0 4.334267367951127E-5 0.0 0.00238384705237312 0.0 30-31 0.0 4.334267367951127E-5 0.0 0.008235107999107141 0.0 32-33 0.0 4.334267367951127E-5 0.0 0.0215413088187171 0.0 34-35 0.0 4.334267367951127E-5 0.0 0.04336434501635102 0.0 36-37 0.0 4.334267367951127E-5 0.0 0.08031397432813439 0.0 38-39 0.0 4.334267367951127E-5 0.0 0.13048311911216867 0.0 40-41 0.0 4.334267367951127E-5 0.0 0.1792652983384586 0.0 42-43 0.0 6.50140105192669E-5 0.0 0.2272673094385173 0.0 44-45 0.0 8.668534735902254E-5 0.0 0.2926063900103806 0.0 46-47 0.0 1.300280210385338E-4 0.0 0.37185846883336693 0.0 48-49 0.0 1.7337069471804508E-4 0.0 0.4552064303190671 0.0 50-51 0.0 1.7337069471804508E-4 0.0 0.5409815815308199 0.0 52-53 0.0 1.7337069471804508E-4 0.0 0.628945537763388 0.0 54-55 0.0 1.7337069471804508E-4 0.0 0.7272250503316797 0.0 56-57 0.0 1.7337069471804508E-4 0.0 0.8485845366343114 0.0 58-59 0.0 1.7337069471804508E-4 0.0 0.9852439867458104 0.0 60-61 0.0 1.7337069471804508E-4 0.0 1.133280888698181 0.0 62-63 0.0 1.7337069471804508E-4 0.0 1.2817295460505074 0.0 64-65 0.0 1.7337069471804508E-4 0.0 1.4328004351604438 0.0 66-67 0.0 1.7337069471804508E-4 0.0 1.6184154351929507 0.0 68-69 0.0 1.7337069471804508E-4 0.0 1.8259184854336108 0.0 70-71 0.0 1.7337069471804508E-4 0.0 2.0576717615979576 0.0 72-73 0.0 1.7337069471804508E-4 0.0 2.2974000897193347 0.0 74-75 0.0 1.7337069471804508E-4 0.0 2.5388187821142125 0.0 76-77 0.0 1.7337069471804508E-4 0.0 2.8112925002004596 0.0 78-79 0.0 1.7337069471804508E-4 0.0 3.1221461558299146 0.0 80-81 0.0 1.7337069471804508E-4 0.0 3.4592654717091533 0.0 82-83 0.0 1.7337069471804508E-4 0.0 3.81164140872358 0.0 84-85 0.0 1.7337069471804508E-4 0.0 4.165274283274712 0.0 86-87 0.0 1.7337069471804508E-4 0.0 4.538693088360542 0.0 88-89 0.0 1.7337069471804508E-4 0.0 4.956104707231075 0.0 90-91 0.0 1.7337069471804508E-4 0.0 5.415125292833939 0.0 92-93 0.0 1.7337069471804508E-4 0.0 5.89215475935064 0.0 94-95 0.0 1.7337069471804508E-4 0.0 6.373995262645767 0.0 96-97 0.0 1.7337069471804508E-4 0.0 6.866238007623976 0.0 98-99 0.0 1.7337069471804508E-4 0.0 7.387563686641138 0.0 100-101 0.0 1.7337069471804508E-4 0.0 7.96560325416794 0.0 102-103 0.0 1.7337069471804508E-4 0.0 8.574351105996676 0.0 104-105 0.0 1.7337069471804508E-4 0.0 9.18669639974081 0.0 106-107 0.0 1.7337069471804508E-4 0.0 9.80029863102165 0.0 108-109 0.0 1.7337069471804508E-4 0.0 10.418191786996765 0.0 110-111 0.0 1.7337069471804508E-4 0.0 11.096114546018 0.0 112-113 0.0 1.7337069471804508E-4 0.0 11.802231714267759 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1920 0.0 50.812897 1 GTATCAA 3185 0.0 38.289154 1 TCAACGC 3200 0.0 35.132214 4 CAACGCA 3220 0.0 34.914 5 ATCAACG 3245 0.0 34.645016 3 GTACATG 7000 0.0 33.908318 1 AACGCAG 3390 0.0 32.805107 6 ACATGGG 7305 0.0 30.372623 3 TACATGG 7610 0.0 29.936968 2 TATCAAC 4305 0.0 27.219913 2 ACGCAGA 4135 0.0 26.749073 7 CGCAGAG 4225 0.0 25.475527 8 CATGGGG 4620 0.0 24.20525 4 GAGTACA 5345 0.0 21.591627 1 AGTACAT 5505 0.0 19.989784 2 CAGAGTA 4825 0.0 19.35135 10-11 AGAGTAC 4610 0.0 19.22181 10-11 GCAGAGT 5520 0.0 19.177406 9 CGCGGAA 265 0.0 19.077835 82-83 CATGGGA 3795 0.0 18.495407 4 >>END_MODULE