FastQCFastQC Report
Thu 26 May 2016
SRR1364207_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364207_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2307195
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54980.23829801988995294No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52160.22607538591233076No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29670.12859771280710994No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34400.067.498641
GTATCAA64250.051.2437441
TCAACGC75500.042.7202724
ATCAACG75650.042.716083
CAACGCA76300.042.3411675
AACGCAG78150.041.7185976
TATCAAC83300.038.864622
ACGCAGA92450.034.9431157
CGCAGAG93500.034.4234478
GTACATG71800.033.5000721
ACATGGG73050.031.6090933
TACATGG75400.031.5710582
GTGGTAT19350.030.768611
TGGTATC19150.029.522652
GCAGAGT111800.028.6291989
GAGTACT73350.026.19931612-13
CAGAGTA104150.026.07655310-11
CATGGGG44950.025.2863034
AGAGTAC99900.025.07178710-11
GTACTTT79700.023.77599314-15
Produced by FastQC (version 0.11.5)