FastQCFastQC Report
Thu 26 May 2016
SRR1364206_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364206_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1902531
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21350.048.7692221
GTATCAA32550.040.0312161
ATCAACG33750.037.015423
CAACGCA34100.036.112135
TCAACGC34650.035.8804054
AACGCAG37150.033.2969636
GTACATG74500.032.504521
TATCAAC40500.031.8760622
TACATGG75250.030.6750412
GTCTACG1203.6979316E-529.7492241
ACATGGG77600.028.8245983
ACGCAGA45800.026.6174037
CGCAGAG45300.026.517378
CATGGGA45350.025.1847844
GTATAGG7150.021.6357981
GAGTACA57400.020.9384781
CATGGGG37000.020.9004634
GCAGAGT57450.020.2897919
AGAGTAC49050.019.52083610-11
CAGAGTA53150.019.41367510-11
Produced by FastQC (version 0.11.5)