##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364206_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1902531 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.076858143178743 33.0 31.0 34.0 28.0 34.0 2 31.111187150169958 33.0 31.0 34.0 27.0 34.0 3 31.182167333935688 34.0 31.0 34.0 27.0 34.0 4 34.551431750652156 37.0 35.0 37.0 32.0 37.0 5 34.65792410215655 37.0 35.0 37.0 32.0 37.0 6 34.71017187104967 37.0 35.0 37.0 32.0 37.0 7 34.59467046791879 37.0 35.0 37.0 31.0 37.0 8 34.56490275322715 37.0 35.0 37.0 31.0 37.0 9 36.06971292452002 39.0 37.0 39.0 31.0 39.0 10-11 36.03205992438494 39.0 37.0 39.0 31.0 39.0 12-13 35.98744935036538 39.0 37.0 39.0 31.0 39.0 14-15 37.3198197558936 40.0 38.0 41.0 31.0 41.0 16-17 37.36469287491242 40.0 38.0 41.0 31.0 41.0 18-19 37.277612033654115 40.0 38.0 41.0 31.0 41.0 20-21 37.26352895169645 40.0 38.0 41.0 30.5 41.0 22-23 37.31963631604425 40.0 38.0 41.0 30.5 41.0 24-25 37.17991244295099 40.0 38.0 41.0 30.0 41.0 26-27 37.204655797987 40.0 37.5 41.0 30.0 41.0 28-29 37.29791630202084 40.0 38.0 41.0 30.0 41.0 30-31 37.19503309013099 40.0 37.0 41.0 30.0 41.0 32-33 37.01237956175221 40.0 37.0 41.0 30.0 41.0 34-35 36.965724080185815 40.0 37.0 41.0 30.0 41.0 36-37 36.988902151922886 40.0 37.0 41.0 30.0 41.0 38-39 36.832699966518284 40.0 37.0 41.0 30.0 41.0 40-41 36.83885991870829 40.0 37.0 41.0 30.0 41.0 42-43 36.78275833613223 40.0 37.0 41.0 30.0 41.0 44-45 36.78063011851055 40.0 37.0 41.0 30.0 41.0 46-47 36.71647268822427 40.0 36.5 41.0 30.0 41.0 48-49 36.63760905866974 40.0 36.0 41.0 30.0 41.0 50-51 36.54477640574582 40.0 36.0 41.0 30.0 41.0 52-53 36.410392787292295 40.0 36.0 41.0 29.5 41.0 54-55 36.244266716284784 40.0 35.0 41.0 29.0 41.0 56-57 36.06732584120837 39.0 35.0 41.0 29.0 41.0 58-59 35.85643151149705 39.0 35.0 41.0 29.0 41.0 60-61 35.62623447397178 39.0 35.0 41.0 28.0 41.0 62-63 35.348636106323625 38.5 35.0 40.5 28.0 41.0 64-65 35.05451842834624 38.0 34.0 40.0 28.0 41.0 66-67 34.75590463440543 37.0 34.0 40.0 28.0 41.0 68-69 34.53295557339145 37.0 34.0 40.0 28.0 41.0 70-71 34.37271482041554 36.5 34.0 39.0 28.0 41.0 72-73 34.05829103441678 36.0 34.0 39.0 28.0 41.0 74-75 33.71903953207595 35.5 34.0 39.0 27.0 40.5 76-77 33.374882722016096 35.0 34.0 37.5 27.0 39.5 78-79 33.01735188546205 35.0 33.5 37.0 26.0 39.0 80-81 32.703145967135356 35.0 33.0 36.5 26.0 39.0 82-83 32.408554183874 35.0 33.0 36.0 26.0 38.0 84-85 32.131447266825084 35.0 33.0 36.0 26.0 37.0 86-87 31.90510877352327 35.0 33.0 35.5 25.5 37.0 88-89 31.712033601554978 35.0 33.0 35.0 25.0 36.0 90-91 31.54438403368986 35.0 33.0 35.0 25.0 36.0 92-93 31.398185364653717 35.0 33.0 35.0 24.5 36.0 94-95 31.253045548272276 35.0 33.0 35.0 24.0 36.0 96-97 31.14913475785677 35.0 33.0 35.0 24.0 35.0 98-99 31.06647066460415 35.0 32.5 35.0 23.5 35.0 100-101 30.957378618272188 35.0 32.0 35.0 23.0 35.0 102-103 29.989427767537034 34.0 30.5 35.0 19.0 35.0 104-105 30.655526243724808 35.0 32.0 35.0 20.0 35.0 106-107 30.74568587844298 35.0 32.0 35.0 20.0 35.0 108-109 30.706007944154393 35.0 32.0 35.0 19.0 35.0 110-111 30.6234155974331 35.0 32.0 35.0 19.0 35.0 112-113 30.74657311759966 35.0 32.5 35.0 19.0 35.0 114-115 30.722856815473705 35.0 33.0 35.0 18.5 35.0 116-117 30.618721849998764 35.0 33.0 35.0 16.5 35.0 118-119 30.373166061420285 35.0 32.0 35.0 2.0 35.0 120-121 30.306006577553795 35.0 32.0 35.0 2.0 35.0 122-123 30.162322716423542 35.0 32.0 35.0 2.0 35.0 124-125 29.301655268692073 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5557369147917726 1101 2 0.5806293965996083 1101 3 0.5962916181803948 1101 4 0.5318760670479463 1101 5 0.48671621359311246 1101 6 0.5050783565280668 1101 7 0.6158006091502699 1101 8 0.5181403068948285 1101 9 0.6094593257086629 1101 10-11 0.6384661739969602 1101 12-13 0.5890221787124545 1101 14-15 0.6340155461404251 1101 16-17 0.6508874807463272 1101 18-19 0.6885008021942127 1101 20-21 0.7504405516169541 1101 22-23 0.7124923979634303 1101 24-25 0.83702528505016 1101 26-27 0.8866320478154606 1101 28-29 0.8320367466981722 1101 30-31 0.8979815956870851 1101 32-33 1.0380375953982295 1101 34-35 0.9224886395447172 1101 36-37 0.882343775469387 1101 38-39 0.9618745459971976 1101 40-41 0.8915446484962999 1101 42-43 0.8783506495464479 1101 44-45 0.7361430616742126 1101 46-47 0.7601551162852402 1101 48-49 0.6402891815139427 1101 50-51 0.6331378520349418 1101 52-53 0.5719445054556616 1101 54-55 0.6355163258820866 1101 56-57 0.6247636981931706 1101 58-59 0.6779210514989416 1101 60-61 0.6767406347948608 1101 62-63 0.6238827294488232 1101 64-65 0.6553385805739822 1101 66-67 0.6400254452560503 1101 68-69 0.6248352382968463 1101 70-71 0.5675723990055133 1101 72-73 0.5526966189656406 1101 74-75 0.5490485704625101 1101 76-77 0.5400133504543803 1101 78-79 0.4869652391999395 1101 80-81 0.42582672681735545 1101 82-83 0.49079117939230343 1101 84-85 0.40388486501764476 1101 86-87 0.38201228094166595 1101 88-89 0.43004255120072443 1101 90-91 0.458668221243677 1101 92-93 0.46191645112456214 1101 94-95 0.4599761377031726 1101 96-97 0.4275182110130622 1101 98-99 0.35661237157224335 1101 100-101 0.4033191893082986 1101 102-103 0.29768228719124323 1101 104-105 0.4218813119293259 1101 106-107 0.3360401578775871 1101 108-109 0.3457149374730477 1101 110-111 0.3291092955699888 1101 112-113 0.41611637091555664 1101 114-115 0.4512293063705215 1101 116-117 0.5770465669765947 1101 118-119 0.5237740773435036 1101 120-121 0.45511978497362904 1101 122-123 0.4709248604265568 1101 124-125 0.26045611562719984 1102 1 -0.01109020401465699 1102 2 0.07772218219905014 1102 3 0.03483588868470022 1102 4 0.054272890046028976 1102 5 0.1090450735901527 1102 6 0.10705754151707936 1102 7 -0.005697087292119818 1102 8 0.11070005116235393 1102 9 0.14477861555723592 1102 10-11 0.1826778403456899 1102 12-13 0.1335053254572074 1102 14-15 0.1720614075404967 1102 16-17 0.09713064625317003 1102 18-19 0.16923147464806476 1102 20-21 0.23976301087012075 1102 22-23 0.3007443780155086 1102 24-25 0.5488807410422609 1102 26-27 0.45306608778233226 1102 28-29 0.3528422610327553 1102 30-31 0.4005636529593275 1102 32-33 0.541516466997706 1102 34-35 0.5195268813261933 1102 36-37 0.34496598293738856 1102 38-39 0.4084490621014609 1102 40-41 0.4020772620659514 1102 42-43 0.32385245680072927 1102 44-45 0.25979406216720946 1102 46-47 0.3206830641081524 1102 48-49 0.21803868954604866 1102 50-51 0.23491079450257502 1102 52-53 0.21080476675399495 1102 54-55 0.20615366688540604 1102 56-57 0.19518798385909264 1102 58-59 0.19620685615032585 1102 60-61 0.22277607134772381 1102 62-63 0.3126476939024343 1102 64-65 0.2489720659363428 1102 66-67 0.21679597227072378 1102 68-69 0.2557249177476564 1102 70-71 0.29324816842017754 1102 72-73 0.29210764482709095 1102 74-75 0.23684694083691227 1102 76-77 0.10287871351966515 1102 78-79 0.17224617082982974 1102 80-81 0.25785695424980304 1102 82-83 0.24109816901122372 1102 84-85 0.17439185414108493 1102 86-87 0.15205393408592727 1102 88-89 0.1466593397961553 1102 90-91 0.13522998705952816 1102 92-93 0.08987868417571931 1102 94-95 0.09539117858419743 1102 96-97 0.0911771226306044 1102 98-99 0.06222893968017473 1102 100-101 0.036296687196326616 1102 102-103 -0.004826769725088553 1102 104-105 0.11077159090794808 1102 106-107 0.05815468255360656 1102 108-109 -0.10350935116573368 1102 110-111 -0.03782009805635411 1102 112-113 -0.0032791382412789005 1102 114-115 0.04502051874623092 1102 116-117 0.0023345426453467155 1102 118-119 -0.05683536827494606 1102 120-121 -0.04862272196099582 1102 122-123 0.03460321953710732 1102 124-125 -0.0027813923520518813 1103 1 0.15166117159126458 1103 2 -0.10415775797300952 1103 3 0.1497131759024839 1103 4 0.182029014767636 1103 5 0.15427248526751924 1103 6 0.12397121678673528 1103 7 0.27453103402322654 1103 8 0.19544583700453444 1103 9 0.2442076299246736 1103 10-11 0.2398824398915167 1103 12-13 0.20498238231558474 1103 14-15 0.331086910837584 1103 16-17 0.22501505168572322 1103 18-19 0.3555309582499646 1103 20-21 0.41044076972421806 1103 22-23 0.39910156610231695 1103 24-25 0.6034483531650565 1103 26-27 0.560297981096447 1103 28-29 0.5157804624825957 1103 30-31 0.537504949522507 1103 32-33 0.7551873696572144 1103 34-35 0.6535935982133907 1103 36-37 0.49852058685021916 1103 38-39 0.7147360451574869 1103 40-41 0.6091155060940778 1103 42-43 0.5670537155893314 1103 44-45 0.4991377562149779 1103 46-47 0.481670716407379 1103 48-49 0.5043192039789943 1103 50-51 0.45751835924338735 1103 52-53 0.516912702690064 1103 54-55 0.45557381077815506 1103 56-57 0.44055664078734225 1103 58-59 0.5657856314243475 1103 60-61 0.5758891985585137 1103 62-63 0.559819650101403 1103 64-65 0.5648094114536093 1103 66-67 0.4750921860791877 1103 68-69 0.487251130682715 1103 70-71 0.5410010724771226 1103 72-73 0.49198755224662705 1103 74-75 0.5925703075778159 1103 76-77 0.4299320237078845 1103 78-79 0.4823234394878497 1103 80-81 0.4579030807182036 1103 82-83 0.36664636434726816 1103 84-85 0.35282431844083817 1103 86-87 0.3738847181812943 1103 88-89 0.3924188434862721 1103 90-91 0.41778340096406197 1103 92-93 0.40550764177212173 1103 94-95 0.38030864222889704 1103 96-97 0.38559008826229757 1103 98-99 0.30515234790760815 1103 100-101 0.4102981446835514 1103 102-103 0.3230580451139886 1103 104-105 0.38094755383209744 1103 106-107 0.42891268508646974 1103 108-109 0.4246776056882773 1103 110-111 0.530418007319426 1103 112-113 0.5199870251282981 1103 114-115 0.4576029459264568 1103 116-117 0.592831671340921 1103 118-119 0.38231849761439207 1103 120-121 0.3799228750083081 1103 122-123 0.3429054379745935 1103 124-125 0.19534857511377623 1104 1 0.2135071468234635 1104 2 0.294114945845358 1104 3 0.2238875825072313 1104 4 0.30584480424131755 1104 5 0.21259291870405406 1104 6 0.2491737451665088 1104 7 0.2624864003802898 1104 8 0.2194899546515927 1104 9 0.3516844101104297 1104 10-11 0.31077575774497745 1104 12-13 0.323156401923427 1104 14-15 0.3556741141399584 1104 16-17 0.3011915222739532 1104 18-19 0.44884816671229544 1104 20-21 0.45080196683464635 1104 22-23 0.4123897497968585 1104 24-25 0.6052117690577319 1104 26-27 0.4909694207249302 1104 28-29 0.29201820758063235 1104 30-31 0.34157486696316397 1104 32-33 0.44155475871810523 1104 34-35 0.44512997592237014 1104 36-37 0.3656716446417576 1104 38-39 0.4137485580573852 1104 40-41 0.319047136641025 1104 42-43 0.2098329983550613 1104 44-45 0.14403584703025274 1104 46-47 0.14061937481810816 1104 48-49 0.07052921429890802 1104 50-51 0.00542148101841633 1104 52-53 -0.027903473780526156 1104 54-55 0.009324455772997453 1104 56-57 -0.05817465436230407 1104 58-59 0.02042636929844832 1104 60-61 -0.06623928441361215 1104 62-63 0.04382184308384751 1104 64-65 -0.048583906399841226 1104 66-67 0.021671133447604518 1104 68-69 -2.3725941016294883E-4 1104 70-71 -0.002426430102858035 1104 72-73 0.056156798892658344 1104 74-75 -0.10880030645727601 1104 76-77 -0.06308268217446766 1104 78-79 -0.09969732934600017 1104 80-81 -0.03924733207848874 1104 82-83 -0.09365742822239298 1104 84-85 -0.09946154327226964 1104 86-87 -0.03977820751529748 1104 88-89 -0.07534237942394029 1104 90-91 -0.05134134103799681 1104 92-93 0.001655732587398262 1104 94-95 0.053585216016308834 1104 96-97 -0.0378335443486435 1104 98-99 -0.060399122680625084 1104 100-101 -0.02380724747331442 1104 102-103 -0.03199161948869289 1104 104-105 -0.07474710561062992 1104 106-107 -0.00613633452137563 1104 108-109 -0.11455807542627383 1104 110-111 -0.19110224551855381 1104 112-113 0.0120555235803117 1104 114-115 -0.04064782806734968 1104 116-117 0.029146170824926543 1104 118-119 -0.17683849412967234 1104 120-121 -0.1314774087894257 1104 122-123 -0.11715390155069016 1104 124-125 -0.21454242075825647 1105 1 0.19133852730512402 1105 2 0.1922748125171161 1105 3 0.21972234708364624 1105 4 0.26144344551455134 1105 5 0.17153723380653219 1105 6 0.09866702150456774 1105 7 0.16042261146638026 1105 8 0.15619771389742 1105 9 0.1255144165987332 1105 10-11 0.18004461516772352 1105 12-13 0.1487159861632108 1105 14-15 0.1922426929473815 1105 16-17 0.2568913047250021 1105 18-19 0.3740344827562154 1105 20-21 0.37724535958047056 1105 22-23 0.33881571318246984 1105 24-25 0.6182611437635259 1105 26-27 0.4866596615554357 1105 28-29 0.40503005191227714 1105 30-31 0.3710514772706617 1105 32-33 0.553967389279272 1105 34-35 0.5648402923225149 1105 36-37 0.5304513604286853 1105 38-39 0.514886809778055 1105 40-41 0.49495696196576233 1105 42-43 0.4166520765072974 1105 44-45 0.29963550607634915 1105 46-47 0.2769184982612316 1105 48-49 0.2227900694825493 1105 50-51 0.1015020377401683 1105 52-53 0.07182821584964216 1105 54-55 0.08316960163256937 1105 56-57 0.22965799833543343 1105 58-59 0.2398041284170631 1105 60-61 0.17756400553714968 1105 62-63 0.1499279713486743 1105 64-65 0.19169763501714954 1105 66-67 0.18540763205452748 1105 68-69 0.2627824726611365 1105 70-71 0.26847862441159975 1105 72-73 0.2643993596091718 1105 74-75 0.2512292089556283 1105 76-77 0.21853443167614728 1105 78-79 0.2712344417059924 1105 80-81 0.20213136848001056 1105 82-83 0.2130598726439885 1105 84-85 0.22386364787819701 1105 86-87 0.22984585263507995 1105 88-89 0.19672090060353753 1105 90-91 0.19862456357124003 1105 92-93 0.2632162328227601 1105 94-95 0.20347440599633515 1105 96-97 0.1825673309360134 1105 98-99 0.15135577217071372 1105 100-101 0.17408011051556826 1105 102-103 0.10871967651781844 1105 104-105 0.1796653213959729 1105 106-107 0.1802547701438435 1105 108-109 0.047712066934703756 1105 110-111 -0.012747729787601969 1105 112-113 0.05038535831944202 1105 114-115 0.09764155020065601 1105 116-117 0.05719230137973952 1105 118-119 -0.011834741092258128 1105 120-121 -0.096424675433191 1105 122-123 -0.12159622563501316 1105 124-125 -0.19671422081678003 1106 1 0.20025212084650335 1106 2 0.3554710184302543 1106 3 0.2588585937262664 1106 4 0.33941456555447047 1106 5 0.34176617373002927 1106 6 0.24715507187383423 1106 7 0.24254889567431803 1106 8 0.15557006910080418 1106 9 0.24288010320583453 1106 10-11 0.25952999311881797 1106 12-13 0.23256984970598182 1106 14-15 0.29966176567221936 1106 16-17 0.37069796004219313 1106 18-19 0.4041952791285297 1106 20-21 0.45922264298855 1106 22-23 0.36743605655085787 1106 24-25 0.5318129286089714 1106 26-27 0.4500730273810589 1106 28-29 0.4847006110141123 1106 30-31 0.5311897303017687 1106 32-33 0.615191156579705 1106 34-35 0.697562398179727 1106 36-37 0.5897688511774106 1106 38-39 0.5926443490369806 1106 40-41 0.40160530244179427 1106 42-43 0.42967337182091825 1106 44-45 0.3816647906339057 1106 46-47 0.4042697023536874 1106 48-49 0.3029906253372303 1106 50-51 0.25587814843803613 1106 52-53 0.3537918051894309 1106 54-55 0.230902273708395 1106 56-57 0.24874086404745555 1106 58-59 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76-77 -2.35900074878219 2216 78-79 -2.3260511227596865 2216 80-81 -2.356828264958885 2216 82-83 -2.3864180654476392 2216 84-85 -2.3637344270732825 2216 86-87 -2.351908599809029 2216 88-89 -2.3522226437452254 2216 90-91 -2.372606794680948 2216 92-93 -2.375813980599194 2216 94-95 -2.3225124773772094 2216 96-97 -2.382127834450557 2216 98-99 -2.3110858306087145 2216 100-101 -2.3672378398694214 2216 102-103 -2.381191675331216 2216 104-105 -2.3630981999689844 2216 106-107 -2.3161422290100298 2216 108-109 -2.3700898979090965 2216 110-111 -2.410853436050143 2216 112-113 -2.4300232143786467 2216 114-115 -2.4784076121429237 2216 116-117 -2.3483548762223982 2216 118-119 -2.2124332945702108 2216 120-121 -2.093186500262579 2216 122-123 -2.152559923236204 2216 124-125 -2.0232018899440405 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 30023.0 3 6099.0 4 4198.0 5 3356.0 6 3672.0 7 4079.0 8 4330.0 9 4611.0 10 5254.0 11 6235.0 12 6769.0 13 6698.0 14 6525.0 15 6376.0 16 6048.0 17 5718.0 18 5164.0 19 5099.0 20 4849.0 21 5178.0 22 6001.0 23 7994.0 24 12532.0 25 18479.0 26 22095.0 27 20383.0 28 22784.0 29 27783.0 30 32496.0 31 39526.0 32 51194.0 33 67340.0 34 96100.0 35 148328.0 36 290022.0 37 601215.0 38 306387.0 39 1591.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.1788678776888 18.94968427098312 12.651829208642024 24.219618642686058 2 15.649077156811034 20.678239916616224 39.845674808539684 23.827008118033056 3 19.03937318416659 25.150038781600433 29.35459529099347 26.455992743239516 4 12.212971352218009 16.960660635868333 37.924617299518374 32.901750712395284 5 13.132201753477272 38.81628452753237 34.68205144115261 13.369462277837755 6 31.54649048618014 37.355389885531395 17.78450129649088 13.313618331797588 7 27.5030485881883 33.14330466959611 21.443821878350818 17.909824863864767 8 25.028921999168475 36.70347552812543 20.16182653528379 18.105775937422308 9 25.646381577218353 16.919822929029692 19.859249393171353 37.574546100580605 10-11 24.066382734917166 27.67590780847354 28.29392586674306 19.96378358986624 12-13 24.525619191791765 25.204916740254824 27.95627286489214 22.313191203061265 14-15 21.92057406484092 26.799820328764717 25.512080104564667 25.767525501829695 16-17 22.13737629226878 29.46927359619189 26.3369213043268 22.056428807212527 18-19 22.03410232695541 28.59376852080362 27.661877244343593 21.710251907897383 20-21 22.983242707912797 27.943144639012246 27.4313791044274 21.642233548647557 22-23 22.562169183872324 27.933215522904035 27.153017137711245 22.351598155512395 24-25 22.27818505897386 27.83643012468639 27.5932703725138 22.292114443825945 26-27 22.173699790717443 28.165247530113607 27.254203393626263 22.40684928554268 28-29 22.132387360145543 28.28602284665073 27.316841881443736 22.264747911759983 30-31 22.38582134331171 27.98115308688246 27.32092764828067 22.31209792152516 32-33 22.059875562358375 28.0714793698117 27.370210131097373 22.498434936732547 34-35 22.501771662650196 28.108374234031903 27.24709384239457 22.142760260923325 36-37 22.56752043204245 27.91463975916567 27.096779789724696 22.42106001906719 38-39 22.183680910536516 28.056553116864645 27.365438318990094 22.39432765360874 40-41 22.581426001390337 27.950095076396213 27.244624274114383 22.223854648099064 42-43 22.278834911039354 27.944568681657305 27.212215936044103 22.56438047125924 44-45 22.393757554111684 28.102097030601726 27.26486689914737 22.239278516139223 46-47 22.451708024746516 28.113104405279397 26.87761827920252 22.557569290771568 48-49 22.209995398076863 28.047446852518483 27.18645883460173 22.55609891480292 50-51 22.286351494406816 28.119379088711977 27.15769405843067 22.43657535845054 52-53 22.424769164904287 27.998451506692923 27.106201883895064 22.470577444507725 54-55 22.223127508830267 27.952295133713605 27.325641142227564 22.498936215228564 56-57 22.21946558064333 28.224639890332387 27.067647633925475 22.488246895098808 58-59 22.22329398165609 27.91762194161983 27.221176891733517 22.637907184990556 60-61 22.24177249926139 27.93972615126132 27.371705030653686 22.446796318823605 62-63 22.127972525535867 27.967925781517977 27.39059090021398 22.513510792732177 64-65 22.05181523087205 27.94349350305642 27.287347011505826 22.717344254565706 66-67 22.019681530621014 28.127274213399765 27.094786257778487 22.758257998200733 68-69 22.18322603028666 27.866673500418564 27.348911659300672 22.6011888099941 70-71 22.016840689932668 27.67207777445754 27.363637534359835 22.94744400124995 72-73 22.02776930921018 27.821320816206384 27.309430947299813 22.841478927283625 74-75 21.982222916044133 27.965306802003987 27.182313007767917 22.870157274183963 76-77 22.004665913105317 27.72804407203808 27.36273939611195 22.90455061874465 78-79 22.147593680706475 27.81717560873175 27.242656151155913 22.792574559405864 80-81 22.11239458474115 27.827604658350484 27.207386584640226 22.85261417226814 82-83 22.132293789147493 27.863961139563433 27.24080755826345 22.762937513025623 84-85 22.141606401931135 27.84304376647019 27.178164759415047 22.837185072183637 86-87 22.06273578880472 27.72760699561762 27.29391591949289 22.915741296084768 88-89 22.04680600328727 27.65413870551819 27.285437881177888 23.013617410016657 90-91 22.0519359763429 27.799536981433263 27.254390305887128 22.894136736336705 92-93 22.10073748495056 27.53293793704479 27.3593224000574 23.007002177947253 94-95 21.98731584399939 27.44914537529218 27.436004984938485 23.12753379576995 96-97 21.98807355604088 27.373948420363785 27.499126147506303 23.13885187608903 98-99 21.808253358723324 27.422949409640694 27.341248602832085 23.427548628803898 100-101 21.770377373815116 27.511297873531632 27.415857685563616 23.30246706708964 102-103 21.805820863983246 27.302068231779046 27.514352937713348 23.377757966524353 104-105 21.624036964579116 27.431123138950724 27.427627285217245 23.517212611252916 106-107 21.586673231974167 27.45099482548261 27.406592245243154 23.555739697300066 108-109 21.633031187828678 27.49222735288475 27.250861364359096 23.623880094927475 110-111 21.515521205323118 27.687209526022162 27.249310641715557 23.547958626939163 112-113 21.535872662568476 27.700323017061894 27.372811210471546 23.390993109898083 114-115 21.38305538168151 27.745574931010488 27.30354781051459 23.567821876793417 116-117 21.368650670318406 27.63030229406503 27.447794152061544 23.55325288355502 118-119 21.292992691450166 27.64551489154285 27.300304670176267 23.761187746830714 120-121 21.347199174027544 27.7488540658331 27.272588539053327 23.63135822108603 122-123 21.369978095890737 27.665944065006286 27.256960462465063 23.707117376637914 124-125 21.269466786825998 27.650707588335827 27.212159435047255 23.86766618979092 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1501.0 1 1209.5 2 838.5 3 685.0 4 535.5 5 420.5 6 370.5 7 344.0 8 353.0 9 383.5 10 365.0 11 330.5 12 333.0 13 348.5 14 395.0 15 465.0 16 526.5 17 656.0 18 849.5 19 1092.0 20 1480.0 21 2001.5 22 2734.5 23 3921.5 24 5450.5 25 7330.0 26 10242.0 27 13805.0 28 17015.5 29 20697.5 30 24689.0 31 28791.5 32 33705.5 33 37680.5 34 40952.5 35 44718.0 36 49665.0 37 54102.5 38 56315.0 39 58383.0 40 60812.0 41 63397.5 42 64718.5 43 66541.5 44 69106.5 45 71034.0 46 71676.5 47 71457.0 48 71789.0 49 71146.5 50 69444.5 51 67358.0 52 65467.5 53 63723.5 54 61659.0 55 57377.5 56 51468.5 57 46380.5 58 42197.5 59 37247.0 60 31135.0 61 24883.0 62 19643.0 63 15096.5 64 11054.0 65 8265.5 66 6163.0 67 4790.5 68 3587.0 69 2448.5 70 1750.0 71 1250.0 72 975.5 73 790.5 74 584.0 75 433.5 76 289.0 77 159.0 78 92.5 79 54.0 80 35.5 81 24.5 82 15.0 83 11.5 84 11.0 85 9.0 86 8.0 87 6.0 88 5.0 89 5.5 90 4.5 91 3.0 92 2.0 93 2.0 94 1.5 95 1.5 96 2.0 97 1.5 98 0.5 99 1.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06396742024177267 2 0.05056422208100683 3 0.044992696571041414 4 0.044572204079723274 5 0.043310726605768846 6 0.0046254174044995855 7 5.781771755624482E-4 8 0.0 9 8.935465440510562E-4 10-11 0.004126082571059289 12-13 0.0026806396321531686 14-15 0.008804061536973641 16-17 0.003311378369130385 18-19 0.005755490974917097 20-21 0.010249504475879762 22-23 0.016951103556262684 24-25 0.004099801790351905 26-27 0.016714576529896226 28-29 0.007963076554337353 30-31 0.007910514992922587 32-33 0.0027332011935679366 34-35 0.019079846793560788 36-37 0.016320364819285466 38-39 0.0279364698919492 40-41 0.005965737220576169 42-43 0.0017082507459799604 44-45 3.942117106107601E-4 46-47 0.0021550240180054888 48-49 0.0034427822726673047 50-51 0.0019447777723464164 52-53 0.0019184969916390324 54-55 0.006596475957553386 56-57 0.0028908858778122406 58-59 0.008173322799996425 60-61 0.016451768722822387 62-63 0.013429478941473226 64-65 0.0031274129041786967 66-67 0.004599136623792201 68-69 0.01095908555497913 70-71 0.002917166658519625 72-73 0.003731870860448529 74-75 0.0013403198160765843 76-77 4.993348334402961E-4 78-79 0.003758151641155913 80-81 0.0028908858778122406 82-83 0.0016031276231504243 84-85 0.012089159125396643 86-87 0.007358618598067522 88-89 0.0021024624565907203 90-91 8.147042019289042E-4 92-93 0.002706920412860553 94-95 0.0 96-97 0.0018922162109316486 98-99 0.0246513723035262 100-101 0.015374256713819643 102-103 0.006990687668164146 104-105 0.014585833292598122 106-107 0.032299079489374945 108-109 0.0013666005967839683 110-111 0.0019447777723464164 112-113 0.016267803257870698 114-115 0.03214139480513064 116-117 0.0510898376951545 118-119 0.3613344539458227 120-121 0.11697575492856621 122-123 0.019789427872660154 124-125 0.323779218314971 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1902531.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.80881960856236 #Duplication Level Percentage of deduplicated Percentage of total 1 88.38945447182152 71.4264748171266 2 8.131167339724188 13.141400695256115 3 1.8257589996704935 4.426122889592465 4 0.6607133535959794 2.135658648148231 5 0.2990451746000919 1.2082743784534928 6 0.17273031213521234 0.8374879580559025 7 0.11081377714987725 0.6268311369493502 8 0.0742176344114387 0.47979515447425436 9 0.049546922701335226 0.3603445504858423 >10 0.2659860744007891 3.878838933425352 >50 0.01532122275626789 0.8348369337169586 >100 0.005244717032815974 0.6439339043153841 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 5.2561561414768014E-5 0.0 0.0 0.0 0.0 7 5.2561561414768014E-5 0.0 0.0 0.0 0.0 8 5.2561561414768014E-5 0.0 0.0 0.0 0.0 9 5.2561561414768014E-5 0.0 0.0 5.2561561414768014E-5 0.0 10-11 1.5768468424430404E-4 0.0 0.0 5.2561561414768014E-5 0.0 12-13 1.5768468424430404E-4 0.0 0.0 1.0512312282953603E-4 2.6280780707384007E-5 14-15 2.1024624565907206E-4 0.0 0.0 1.0512312282953603E-4 5.2561561414768014E-5 16-17 2.1024624565907206E-4 0.0 0.0 1.0512312282953603E-4 5.2561561414768014E-5 18-19 2.1024624565907206E-4 0.0 0.0 1.0512312282953603E-4 5.2561561414768014E-5 20-21 2.1024624565907206E-4 0.0 0.0 1.3140390353692002E-4 5.2561561414768014E-5 22-23 2.1024624565907206E-4 0.0 0.0 2.6280780707384004E-4 5.2561561414768014E-5 24-25 2.6280780707384004E-4 0.0 0.0 6.570195176846002E-4 5.2561561414768014E-5 26-27 3.153693684886081E-4 0.0 0.0 0.0012614774739544323 5.2561561414768014E-5 28-29 3.6793092990337607E-4 0.0 0.0 0.0028646050971048564 5.2561561414768014E-5 30-31 3.6793092990337607E-4 0.0 0.0 0.007910514992922585 5.2561561414768014E-5 32-33 3.6793092990337607E-4 0.0 0.0 0.021891890329250874 5.2561561414768014E-5 34-35 3.6793092990337607E-4 0.0 0.0 0.04381006143920914 5.2561561414768014E-5 36-37 3.6793092990337607E-4 0.0 0.0 0.08104992770157227 5.2561561414768014E-5 38-39 3.6793092990337607E-4 0.0 0.0 0.13463643956392826 5.2561561414768014E-5 40-41 3.6793092990337607E-4 0.0 0.0 0.19050937934782666 5.2561561414768014E-5 42-43 3.6793092990337607E-4 0.0 0.0 0.2463034767896029 5.2561561414768014E-5 44-45 4.204924913181441E-4 0.0 0.0 0.31626291503265913 5.2561561414768014E-5 46-47 4.993348334402961E-4 0.0 0.0 0.40280552590207463 5.2561561414768014E-5 48-49 5.256156141476801E-4 0.0 0.0 0.49407867729881927 5.2561561414768014E-5 50-51 5.256156141476801E-4 0.0 0.0 0.587480571932862 5.2561561414768014E-5 52-53 5.256156141476801E-4 0.0 0.0 0.679936358461439 5.2561561414768014E-5 54-55 5.256156141476801E-4 0.0 0.0 0.7903944797745739 5.2561561414768014E-5 56-57 5.256156141476801E-4 0.0 0.0 0.9191440244600482 5.2561561414768014E-5 58-59 5.256156141476801E-4 0.0 0.0 1.06660548500918 5.2561561414768014E-5 60-61 5.256156141476801E-4 0.0 0.0 1.2208999485422314 5.2561561414768014E-5 62-63 5.256156141476801E-4 0.0 0.0 1.3766924165756036 5.2561561414768014E-5 64-65 5.256156141476801E-4 0.0 0.0 1.5423664581549525 5.2561561414768014E-5 66-67 6.307387369772162E-4 0.0 0.0 1.7398402443902359 5.2561561414768014E-5 68-69 6.833002983919842E-4 0.0 0.0 1.9606776446743837 5.2561561414768014E-5 70-71 7.095810790993681E-4 0.0 0.0 2.211317450280705 5.2561561414768014E-5 72-73 7.358618598067521E-4 0.0 0.0 2.465426318940401 5.2561561414768014E-5 74-75 7.358618598067521E-4 0.0 0.0 2.7257637326277466 5.2561561414768014E-5 76-77 7.358618598067521E-4 0.0 0.0 3.0152728129002893 5.2561561414768014E-5 78-79 7.358618598067521E-4 0.0 0.0 3.353637864507858 5.2561561414768014E-5 80-81 7.884234212215201E-4 0.0 0.0 3.714315298935996 5.2561561414768014E-5 82-83 8.409849826362882E-4 0.0 0.0 4.095202653728112 5.2561561414768014E-5 84-85 8.409849826362882E-4 0.0 0.0 4.474276634651419 5.2561561414768014E-5 86-87 8.935465440510562E-4 0.0 0.0 4.8719048467541395 5.2561561414768014E-5 88-89 9.461081054658242E-4 0.0 0.0 5.3155244250947815 5.2561561414768014E-5 90-91 9.986696668805922E-4 0.0 0.0 5.801797710523507 5.2561561414768014E-5 92-93 0.0010512312282953602 0.0 0.0 6.296191757190815 5.2561561414768014E-5 94-95 0.0010512312282953602 0.0 0.0 6.799836638667123 5.2561561414768014E-5 96-97 0.0010512312282953602 0.0 0.0 7.314388044136995 5.2561561414768014E-5 98-99 0.0011037927897101282 0.0 0.0 7.859425155227431 5.2561561414768014E-5 100-101 0.0011037927897101282 0.0 0.0 8.475840866719123 5.2561561414768014E-5 102-103 0.0011037927897101282 0.0 0.0 9.114647803373506 5.2561561414768014E-5 104-105 0.0012614774739544323 0.0 0.0 9.753717547834963 5.2561561414768014E-5 106-107 0.0012614774739544323 0.0 0.0 10.38847724426041 5.2561561414768014E-5 108-109 0.0012614774739544323 0.0 0.0 11.04788831298938 5.2561561414768014E-5 110-111 0.0012614774739544323 0.0 0.0 11.758021288483604 5.2561561414768014E-5 112-113 0.0013140390353692003 0.0 0.0 12.493410094237623 5.2561561414768014E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2135 0.0 48.769222 1 GTATCAA 3255 0.0 40.031216 1 ATCAACG 3375 0.0 37.01542 3 CAACGCA 3410 0.0 36.11213 5 TCAACGC 3465 0.0 35.880405 4 AACGCAG 3715 0.0 33.296963 6 GTACATG 7450 0.0 32.50452 1 TATCAAC 4050 0.0 31.876062 2 TACATGG 7525 0.0 30.675041 2 GTCTACG 120 3.6979316E-5 29.749224 1 ACATGGG 7760 0.0 28.824598 3 ACGCAGA 4580 0.0 26.617403 7 CGCAGAG 4530 0.0 26.51737 8 CATGGGA 4535 0.0 25.184784 4 GTATAGG 715 0.0 21.635798 1 GAGTACA 5740 0.0 20.938478 1 CATGGGG 3700 0.0 20.900463 4 GCAGAGT 5745 0.0 20.289791 9 AGAGTAC 4905 0.0 19.520836 10-11 CAGAGTA 5315 0.0 19.413675 10-11 >>END_MODULE