FastQCFastQC Report
Thu 26 May 2016
SRR1364206_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364206_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1902531
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64450.33875926331817985No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60260.31673596908539203No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33610.1766594079150353No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38500.066.022191
GTATCAA66350.051.049811
ATCAACG81350.040.5974123
TCAACGC82900.039.8383564
CAACGCA83250.039.6625255
TATCAAC85700.038.7470932
AACGCAG86650.038.2435536
TGGTATC18700.038.1878662
GTGGTAT20600.035.832511
ACGCAGA103050.032.0400927
CGCAGAG103900.031.7779738
GTACATG70700.028.4590281
GCAGAGT117750.027.7875679
TACATGG72150.027.0534882
GAGTACT80650.025.96560912-13
ACATGGG71050.025.9633393
CAGAGTA113200.025.3045510-11
AGAGTAC108400.024.4493410-11
GTACTTT86300.024.0243814-15
AGTACTT86750.022.90536112-13
Produced by FastQC (version 0.11.5)