FastQCFastQC Report
Thu 26 May 2016
SRR1364205_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364205_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2841251
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25600.051.6058271
GTATCAA43450.049.1688881
ATCAACG50650.040.995133
TCAACGC51750.039.778834
CAACGCA52700.039.064515
AACGCAG55900.037.020286
TATCAAC57700.036.3999252
GTACATG128300.034.97281
TACATGG127550.034.8917472
ACATGGG126100.034.3009073
ACGCAGA66950.030.4660247
CGCAGAG69300.029.2612868
CATGGGG74100.027.6201844
CATGGGA61900.025.470744
GTATAGC8900.024.0712261
GCAGAGT90850.021.6011339
GAGTACA93150.021.0183561
AGTACAT87700.020.7598112
AGAGTAC70700.020.4817510-11
ATGGGAG33950.020.1546365
Produced by FastQC (version 0.11.5)