FastQCFastQC Report
Thu 26 May 2016
SRR1364205_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364205_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2841251
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51460.18111740215841543No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42280.14880769069680924No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38400.068.378261
GTATCAA75750.055.33511
ATCAACG93100.045.1214183
TCAACGC95250.044.290344
CAACGCA96850.043.4926155
TATCAAC98100.042.6411972
AACGCAG101650.041.43746
ACGCAGA119600.035.0187347
CGCAGAG122900.034.1746568
GTACATG121050.031.6761041
TACATGG118750.031.568212
ACATGGG116500.031.3085023
GCAGAGT147750.028.4671029
GAGTACT79200.025.8411212-13
GTGGTAT21750.025.4585931
GTACTTT81850.025.07738914-15
CATGGGG66600.024.9262964
TGGTATC20700.024.721292
CATGGGA58050.024.6000964
CAGAGTA136550.024.35406310-11
Produced by FastQC (version 0.11.5)