FastQCFastQC Report
Thu 26 May 2016
SRR1364204_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364204_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1923390
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18550.050.9908031
GTATCAA30950.045.169561
TCAACGC32350.040.819924
ATCAACG32750.040.3213583
CAACGCA33050.039.9574285
AACGCAG34700.037.701836
GTACATG74800.035.5504071
TATCAAC39250.034.403472
TACATGG76850.033.5940132
ACATGGG75900.033.150653
ACGCAGA42500.030.6409247
CGCAGAG43850.029.5619818
CATGGGG44300.029.0030384
CATGGGA37800.025.3353864
GAGTACA56200.024.0285851
GCAGAGT54750.023.7864289
AGTACAT54850.023.5341852
ATGGGGA28150.022.6110865
CAGAGTA51150.020.34518610-11
GCGTGCC2055.9657512E-520.3054879
Produced by FastQC (version 0.11.5)