FastQCFastQC Report
Thu 26 May 2016
SRR1364204_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364204_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1923390
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51040.26536479861078616No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48290.2510671262718429No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24800.12893900872937886No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33900.068.490761
GTATCAA59550.050.286721
ATCAACG71150.041.733943
TCAACGC71950.041.3526154
CAACGCA73700.040.3623055
AACGCAG75850.039.2171976
TATCAAC75850.039.1509742
GTGGTAT16050.037.463941
TGGTATC17050.034.205772
ACGCAGA88500.033.5417377
CGCAGAG91200.032.2225848
GTACATG72850.031.1359771
ATACCGT1152.7673168E-531.0395586
ACATGGG74950.029.1378963
TACATGG75850.029.108242
GCAGAGT101900.027.9633759
GAGTACT63000.027.38400712-13
CATGGGG44600.027.35164
CAGAGTA97000.026.12559110-11
GAGTACA56300.024.6385171
Produced by FastQC (version 0.11.5)