FastQCFastQC Report
Thu 26 May 2016
SRR1364203_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364203_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1834933
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28550.15559151206065835No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19800.044.777151
GTATCAA30100.041.1180231
ATCAACG31800.036.669513
TCAACGC32500.035.8797074
CAACGCA33100.035.4109885
GTACATG69750.034.4645421
AACGCAG34300.034.1534966
TACATGG71950.032.3321042
ACATGGG71950.030.3466993
TATCAAC40200.029.6000182
ACGCAGA42100.027.2600387
CGCAGAG42200.026.7727138
CATGGGG33250.025.9449314
CATGGGA40350.024.623464
GAGTACA53350.022.5295411
AGTACAT51300.021.8036162
GCAGAGT51750.020.7995999
CCTATAC7000.020.3980853
ATAGCCC8850.020.1675973
TAGGACA13000.020.136574
Produced by FastQC (version 0.11.5)