FastQCFastQC Report
Thu 26 May 2016
SRR1364203_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364203_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1834933
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64460.3512934804704041No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63070.343718272002302No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33940.18496588158804708No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35200.075.074121
GTATCAA65500.050.4314731
ATCAACG80750.040.8961143
TCAACGC81550.040.276034
CAACGCA82600.039.7564665
AACGCAG85400.038.5912486
TATCAAC87350.037.6708682
TGGTATC16050.033.3665162
ACGCAGA100800.032.6363457
CGCAGAG101900.032.225668
GTACATG69650.032.044991
GTGGTAT17850.031.3429321
TACATGG70150.030.79092
ACATGGG69200.030.6968423
GCAGAGT113500.028.6176329
GAGTACT79000.026.80844912-13
CAGAGTA111700.025.77680610-11
AGAGTAC108250.024.51002910-11
GTACTTT85350.024.5002514-15
CATGGGA41700.024.2574124
Produced by FastQC (version 0.11.5)