FastQCFastQC Report
Thu 26 May 2016
SRR1364202_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364202_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3204779
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27900.047.9824641
GTATCAA44850.045.6352271
ATCAACG50000.039.617793
CAACGCA51350.038.4621935
TCAACGC53400.036.9833374
AACGCAG54750.036.0624166
TATCAAC56050.035.661542
GTACATG126650.033.9654461
TACATGG122800.033.7653162
ACATGGG124450.032.885893
ACGCAGA69000.028.4419217
CGCAGAG68400.028.3436378
CATGGGG88150.028.2748974
TAAGGTA10050.024.2676164
GTATAGG9100.023.537771
CATGGGA44550.022.1650984
TAAGGTG15700.021.2189945
GGTAAGG13950.020.4683633
GTGTAAG15800.019.9582751
ATGGGGA45300.019.8296555
Produced by FastQC (version 0.11.5)