FastQCFastQC Report
Thu 26 May 2016
SRR1364202_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364202_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3204779
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67170.20959323560220533No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61070.19055916180179663No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33160.10347047331500862No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47600.071.664021
GTATCAA88900.053.639441
ATCAACG109050.043.4354483
CAACGCA112600.042.112315
TCAACGC113550.041.8713074
TATCAAC113250.041.720162
AACGCAG116750.040.817956
ACGCAGA138300.034.3270077
CGCAGAG139700.033.8126568
TGGTATC22500.030.9433272
TACATGG119100.029.4784012
GTACATG122800.029.0874981
ACATGGG118350.028.5083243
GTGGTAT27300.028.3487681
GCAGAGT171300.027.3320759
CAGAGTA154350.025.92035310-11
GAGTACT102900.025.55419212-13
AGAGTAC144000.024.89142810-11
CATGGGG87050.024.0619474
GTACTTT110900.023.7644314-15
Produced by FastQC (version 0.11.5)