FastQCFastQC Report
Thu 26 May 2016
SRR1364201_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364201_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2003326
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39520.19727193676915292No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23950.060.6108471
GTATCAA44900.045.71291
TCAACGC50200.039.4605524
ATCAACG51700.038.4316863
CAACGCA52500.037.732755
AACGCAG53000.037.2552386
GTACATG110350.036.2834661
TATCAAC58800.034.8028342
TACATGG113700.034.47932
ACATGGG114200.032.8176843
ACGCAGA63550.030.7881627
CGCAGAG63800.030.387878
CATGGGA63050.029.1538584
CATGGGG50750.026.4907994
GTATAGA5550.025.7267111
GCAGAGT76850.024.3008639
GTATAGG9300.023.6692661
TGGTATC14700.023.4716782
ATGGGAG32000.023.051825
TACGTTG1300.002054983722.8802175
Produced by FastQC (version 0.11.5)