##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364201_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2003326 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.95507221490661 31.0 31.0 34.0 27.0 34.0 2 30.98699662461327 33.0 31.0 34.0 27.0 34.0 3 31.075870826815006 33.0 31.0 34.0 27.0 34.0 4 34.405683847761175 37.0 35.0 37.0 31.0 37.0 5 34.55512582575177 37.0 35.0 37.0 31.0 37.0 6 34.62436767655389 37.0 35.0 37.0 31.0 37.0 7 34.56257493787831 37.0 35.0 37.0 31.0 37.0 8 34.53241659120882 37.0 35.0 37.0 31.0 37.0 9 36.01855614113729 39.0 37.0 39.0 31.0 39.0 10-11 35.99803626569016 39.0 37.0 39.0 31.0 39.0 12-13 35.9586555058937 39.0 37.0 39.0 31.0 39.0 14-15 37.26778916661591 40.0 38.0 41.0 31.0 41.0 16-17 37.315579191804034 40.0 38.0 41.0 31.0 41.0 18-19 37.22817155071117 40.0 38.0 41.0 30.5 41.0 20-21 37.20356372352777 40.0 38.0 41.0 30.5 41.0 22-23 37.270313468701545 40.0 38.0 41.0 30.5 41.0 24-25 37.13455498505984 40.0 37.0 41.0 30.0 41.0 26-27 37.163783877411866 40.0 37.0 41.0 30.0 41.0 28-29 37.258750198420024 40.0 37.0 41.0 30.0 41.0 30-31 37.14953507317331 40.0 37.0 41.0 30.0 41.0 32-33 36.96111865966897 40.0 37.0 41.0 30.0 41.0 34-35 36.921312107964454 40.0 37.0 41.0 30.0 41.0 36-37 36.945335157632854 40.0 37.0 41.0 30.0 41.0 38-39 36.779174982004925 40.0 37.0 41.0 30.0 41.0 40-41 36.78276850597456 40.0 37.0 41.0 30.0 41.0 42-43 36.71435377966442 40.0 36.0 41.0 30.0 41.0 44-45 36.70530283139139 40.0 36.0 41.0 30.0 41.0 46-47 36.64084652223353 40.0 36.0 41.0 30.0 41.0 48-49 36.54782671417433 40.0 36.0 41.0 30.0 41.0 50-51 36.43582721933424 40.0 35.5 41.0 30.0 41.0 52-53 36.291582847724236 40.0 35.0 41.0 29.5 41.0 54-55 36.10472134839762 39.0 35.0 41.0 29.0 41.0 56-57 35.908945174175344 39.0 35.0 41.0 29.0 41.0 58-59 35.67165354016271 39.0 35.0 41.0 28.5 41.0 60-61 35.42258224572536 38.5 35.0 40.5 28.0 41.0 62-63 35.11815376029662 38.0 34.0 40.0 28.0 41.0 64-65 34.805506692370585 37.0 34.0 40.0 28.0 41.0 66-67 34.49519274446595 37.0 34.0 40.0 27.5 41.0 68-69 34.2548057580244 36.0 34.0 39.0 27.5 41.0 70-71 34.08162126383824 36.0 34.0 39.0 28.0 41.0 72-73 33.76896595960917 35.5 34.0 39.0 27.5 40.5 74-75 33.43242637493847 35.0 34.0 37.5 27.0 40.0 76-77 33.103886486772495 35.0 33.5 37.0 27.0 39.0 78-79 32.76727751748842 35.0 33.0 36.5 26.0 39.0 80-81 32.475926035003795 35.0 33.0 36.0 26.0 38.5 82-83 32.19920721839581 35.0 33.0 36.0 26.0 37.0 84-85 31.941353279496198 35.0 33.0 35.5 25.5 37.0 86-87 31.740342809907126 35.0 33.0 35.0 25.0 36.5 88-89 31.560815364049585 35.0 33.0 35.0 25.0 36.0 90-91 31.403345236871083 35.0 33.0 35.0 24.5 36.0 92-93 31.270791423862114 35.0 33.0 35.0 24.0 36.0 94-95 31.123729238276745 35.0 32.5 35.0 23.5 35.5 96-97 31.022193342471468 35.0 32.0 35.0 23.0 35.0 98-99 30.94164130051724 35.0 32.0 35.0 23.0 35.0 100-101 30.82685394189463 35.0 32.0 35.0 20.0 35.0 102-103 29.852209026389115 34.0 30.5 35.0 19.0 35.0 104-105 30.512683407493338 34.5 31.5 35.0 19.0 35.0 106-107 30.597142951272033 35.0 32.0 35.0 19.0 35.0 108-109 30.548692524332033 35.0 32.0 35.0 18.5 35.0 110-111 30.449082925095567 35.0 32.0 35.0 18.0 35.0 112-113 30.584308794474786 35.0 32.0 35.0 18.0 35.0 114-115 30.556474832353796 35.0 32.0 35.0 16.0 35.0 116-117 30.435110910555746 35.0 32.0 35.0 8.5 35.0 118-119 30.179035014770434 35.0 32.0 35.0 2.0 35.0 120-121 30.089823124144544 35.0 32.0 35.0 2.0 35.0 122-123 29.925531341379287 35.0 32.0 35.0 2.0 35.0 124-125 29.020870292703236 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.587148832869044 1101 2 0.6966329677828149 1101 3 0.7021546538129186 1101 4 0.7133492543489552 1101 5 0.5603750825881235 1101 6 0.6197327720873389 1101 7 0.6566186470823112 1101 8 0.5922077256143581 1101 9 0.6726217026101935 1101 10-11 0.7755512617569877 1101 12-13 0.7353135693293851 1101 14-15 0.8238616127935003 1101 16-17 0.8385238305276985 1101 18-19 0.8700921865959117 1101 20-21 0.941400003512328 1101 22-23 0.8676327514541242 1101 24-25 1.1364922962936532 1101 26-27 1.0277609017344247 1101 28-29 1.0084242224803859 1101 30-31 1.1720913829815203 1101 32-33 1.2650090541274395 1101 34-35 1.1576295768868405 1101 36-37 1.0916411126836678 1101 38-39 1.185089846412481 1101 40-41 1.1028843910261443 1101 42-43 0.9938213394177211 1101 44-45 0.9217867475174302 1101 46-47 0.872698645059053 1101 48-49 0.8811785294896026 1101 50-51 0.7590392873918148 1101 52-53 0.688848447705773 1101 54-55 0.7564700667100084 1101 56-57 0.7813513426665324 1101 58-59 0.8178145537393746 1101 60-61 0.666606824892213 1101 62-63 0.6750984475315605 1101 64-65 0.5940562072193671 1101 66-67 0.6893550210317798 1101 68-69 0.6724348408727892 1101 70-71 0.6775929152933813 1101 72-73 0.6938062634474278 1101 74-75 0.6747396721890979 1101 76-77 0.6477172305681194 1101 78-79 0.6947016996637174 1101 80-81 0.6523517432476282 1101 82-83 0.684310609334581 1101 84-85 0.6526491817437545 1101 86-87 0.5770373946271157 1101 88-89 0.6021494593060623 1101 90-91 0.5725472962220941 1101 92-93 0.6445154917257483 1101 94-95 0.552429691355556 1101 96-97 0.5309536633221263 1101 98-99 0.5712973139705753 1101 100-101 0.4677069553260047 1101 102-103 0.354864637924873 1101 104-105 0.4897947314382982 1101 106-107 0.4472891837596329 1101 108-109 0.43007127268536394 1101 110-111 0.3872536725041549 1101 112-113 0.4630414050963374 1101 114-115 0.4526018368908744 1101 116-117 0.4486008418122651 1101 118-119 0.34502064090944273 1101 120-121 0.2800194986691018 1101 122-123 0.2358076997873546 1101 124-125 0.1255198788161671 1102 1 0.19415375383347566 1102 2 0.17240619956018932 1102 3 0.1795007302444489 1102 4 0.3392544918364706 1102 5 0.2978634766581152 1102 6 0.32479421023555943 1102 7 0.40172649391860915 1102 8 0.39902304338760075 1102 9 0.37096535538782405 1102 10-11 0.48929409839911386 1102 12-13 0.47713686907629693 1102 14-15 0.46510534258489855 1102 16-17 0.4789297161434618 1102 18-19 0.6393904117717568 1102 20-21 0.6208647603176729 1102 22-23 0.6132293250679197 1102 24-25 0.7463038731117564 1102 26-27 0.6710110857507345 1102 28-29 0.6239592201529405 1102 30-31 0.6892444840509455 1102 32-33 0.8117391757310273 1102 34-35 0.6838417545827085 1102 36-37 0.6021205847810833 1102 38-39 0.7199368861424702 1102 40-41 0.6074780970419908 1102 42-43 0.59486051171762 1102 44-45 0.46389435183802163 1102 46-47 0.5771075706393063 1102 48-49 0.4481663540986318 1102 50-51 0.42054084545503656 1102 52-53 0.38561299251030334 1102 54-55 0.36313914851245954 1102 56-57 0.386199928183629 1102 58-59 0.32422092689187565 1102 60-61 0.3384585175165711 1102 62-63 0.4108991276778937 1102 64-65 0.4011946496171248 1102 66-67 0.42498302147472344 1102 68-69 0.3413228562374613 1102 70-71 0.3909650887201721 1102 72-73 0.31488968484302404 1102 74-75 0.2580078043721912 1102 76-77 0.2516196791249783 1102 78-79 0.33568330955716164 1102 80-81 0.3511270013339498 1102 82-83 0.30710247623954956 1102 84-85 0.3767255551518076 1102 86-87 0.3474007598232056 1102 88-89 0.4167723173136153 1102 90-91 0.3398148281286879 1102 92-93 0.3284096050577503 1102 94-95 0.28067399035738205 1102 96-97 0.3020028619429951 1102 98-99 0.2543109299249018 1102 100-101 0.27422069429191964 1102 102-103 0.16316548968614697 1102 104-105 0.27463256408942627 1102 106-107 0.2922342093565611 1102 108-109 0.2997674315672292 1102 110-111 0.27362679494903475 1102 112-113 0.3069003711877265 1102 114-115 0.29822624469406733 1102 116-117 0.3238778057761351 1102 118-119 0.22783939248466822 1102 120-121 0.18712201466937373 1102 122-123 0.14560588562954635 1102 124-125 0.09336312247380718 1103 1 0.33074263988019226 1103 2 0.04420990777074252 1103 3 0.2704066882425238 1103 4 0.3877083362690854 1103 5 0.36990208480018083 1103 6 0.37793015708444955 1103 7 0.4374491697829157 1103 8 0.47562374359763027 1103 9 0.5423739880755818 1103 10-11 0.4948094172528954 1103 12-13 0.4504818911829531 1103 14-15 0.47118776797341155 1103 16-17 0.44172911708674434 1103 18-19 0.5465853415832953 1103 20-21 0.642558723979171 1103 22-23 0.6568186246588823 1103 24-25 0.8998323593699098 1103 26-27 0.8252955522616574 1103 28-29 0.7734777347524613 1103 30-31 0.7697163370738238 1103 32-33 0.9106298869543465 1103 34-35 0.9243719188015191 1103 36-37 0.7131041284379123 1103 38-39 0.8818232619464439 1103 40-41 0.7974488358737446 1103 42-43 0.7530981603593574 1103 44-45 0.659144696579574 1103 46-47 0.6012334123499059 1103 48-49 0.4910306771213513 1103 50-51 0.5251895051493776 1103 52-53 0.5193124079664742 1103 54-55 0.40574003122558366 1103 56-57 0.3961777754139817 1103 58-59 0.4247553794481078 1103 60-61 0.4275108070810347 1103 62-63 0.42788550644268497 1103 64-65 0.31462332221287426 1103 66-67 0.3758487341420391 1103 68-69 0.432706064690457 1103 70-71 0.4153996931519899 1103 72-73 0.3749060834259126 1103 74-75 0.4613977387438766 1103 76-77 0.4028664519508496 1103 78-79 0.33067387861249387 1103 80-81 0.3206168881868834 1103 82-83 0.36587551179513156 1103 84-85 0.3586238931601642 1103 86-87 0.470363738875637 1103 88-89 0.40894476672360724 1103 90-91 0.37465550680723325 1103 92-93 0.3236042766468188 1103 94-95 0.4609160035412927 1103 96-97 0.4407036995642635 1103 98-99 0.3554577507549084 1103 100-101 0.39654102945691605 1103 102-103 0.34850466635448285 1103 104-105 0.3203885419153032 1103 106-107 0.35876217043292513 1103 108-109 0.38528639320226077 1103 110-111 0.36351199758814445 1103 112-113 0.3730162441519802 1103 114-115 0.41812137579293207 1103 116-117 0.5517371099636534 1103 118-119 0.4739642959546053 1103 120-121 0.43187923701105646 1103 122-123 0.5682153498713163 1103 124-125 0.4894066154860255 1104 1 0.38770739386898967 1104 2 0.4831509834653751 1104 3 0.3673342134067532 1104 4 0.3881378996294984 1104 5 0.44555429130830504 1104 6 0.36741019377812734 1104 7 0.47358540404295724 1104 8 0.47817304063357824 1104 9 0.542245263701588 1104 10-11 0.5793847057648378 1104 12-13 0.5643610182725993 1104 14-15 0.6687622054340352 1104 16-17 0.5764387526244192 1104 18-19 0.711483856034107 1104 20-21 0.8347491560218003 1104 22-23 0.8568670891446359 1104 24-25 0.9497692808107914 1104 26-27 0.9247834028141071 1104 28-29 0.7584595897984485 1104 30-31 0.870122795132275 1104 32-33 1.0431994195100955 1104 34-35 1.0340679255858234 1104 36-37 0.9008884625610776 1104 38-39 0.9766283415452293 1104 40-41 0.936626641477524 1104 42-43 0.8469672763567644 1104 44-45 0.7454006939240969 1104 46-47 0.7226966107383888 1104 48-49 0.5754425290685532 1104 50-51 0.5772592978657656 1104 52-53 0.7300257109435151 1104 54-55 0.7390706526690138 1104 56-57 0.5550449261951798 1104 58-59 0.6237287905126507 1104 60-61 0.6045578048561993 1104 62-63 0.6002682946439677 1104 64-65 0.6874970348034068 1104 66-67 0.6686226805215867 1104 68-69 0.6967903251586804 1104 70-71 0.6412765586459841 1104 72-73 0.662756744349636 1104 74-75 0.5899635575773416 1104 76-77 0.5225759012720701 1104 78-79 0.5799342859721079 1104 80-81 0.5809554863230133 1104 82-83 0.6223396026967194 1104 84-85 0.5720895233304617 1104 86-87 0.5154945285044512 1104 88-89 0.5565222595801913 1104 90-91 0.6071100311254689 1104 92-93 0.5228433323932755 1104 94-95 0.5460034776593119 1104 96-97 0.578173978757544 1104 98-99 0.6168334358536285 1104 100-101 0.616098890159062 1104 102-103 0.5482628380923238 1104 104-105 0.5052240272236688 1104 106-107 0.5643337301565161 1104 108-109 0.5010832483018852 1104 110-111 0.4736813195566114 1104 112-113 0.46796222149721345 1104 114-115 0.4176410096950285 1104 116-117 0.46713331647563905 1104 118-119 0.32934517365957916 1104 120-121 0.3331945879832432 1104 122-123 0.30120718997539697 1104 124-125 0.273939248983023 1105 1 0.27833765958687096 1105 2 0.24497248085564394 1105 3 0.355967889380743 1105 4 0.3838458292465816 1105 5 0.32047663458708797 1105 6 0.33239961882588887 1105 7 0.2608363148383077 1105 8 0.22159631040953087 1105 9 0.22471567877877874 1105 10-11 0.2756598031071533 1105 12-13 0.350636262763409 1105 14-15 0.3061789812956519 1105 16-17 0.3203253607116281 1105 18-19 0.47945346879311046 1105 20-21 0.6424242779845741 1105 22-23 0.5007750875984982 1105 24-25 0.7698308965515182 1105 26-27 0.6507686786331917 1105 28-29 0.5372449758712392 1105 30-31 0.5529330873440657 1105 32-33 0.6979036312948281 1105 34-35 0.7552719519993403 1105 36-37 0.6393361392478312 1105 38-39 0.6689846232247803 1105 40-41 0.5615610975897667 1105 42-43 0.5607591459358261 1105 44-45 0.4670264263916124 1105 46-47 0.490662096161536 1105 48-49 0.41206014504041377 1105 50-51 0.32047404745671315 1105 52-53 0.3807072058717367 1105 54-55 0.3936494293338484 1105 56-57 0.42567735248365324 1105 58-59 0.4690453948565434 1105 60-61 0.3752460757931857 1105 62-63 0.4408587405433906 1105 64-65 0.41300371911354006 1105 66-67 0.4310403087677841 1105 68-69 0.4426682830677535 1105 70-71 0.41031838147847566 1105 72-73 0.40538200271188884 1105 74-75 0.2735776035831421 1105 76-77 0.39807230313245867 1105 78-79 0.4044939799893754 1105 80-81 0.3029749307405396 1105 82-83 0.24290051840539917 1105 84-85 0.24016002253352298 1105 86-87 0.23120614760088287 1105 88-89 0.2910895227725021 1105 90-91 0.18565592404945974 1105 92-93 0.10712353040490541 1105 94-95 0.15144039922665442 1105 96-97 0.19211069987992246 1105 98-99 0.2851675462027998 1105 100-101 0.30515222646407736 1105 102-103 0.2672000598784834 1105 104-105 0.186934115257948 1105 106-107 0.26716267792633275 1105 108-109 0.2747293834466511 1105 110-111 0.1908909821164002 1105 112-113 0.21085144990433946 1105 114-115 0.14714708244165564 1105 116-117 0.17259388947188015 1105 118-119 0.09745098850154932 1105 120-121 0.05546256979856068 1105 122-123 0.05725041717877488 1105 124-125 0.007124931362636033 1106 1 0.2322982071073234 1106 2 0.2445878384461544 1106 3 0.26499508634168123 1106 4 0.24664805233010867 1106 5 0.28440331906924854 1106 6 0.16433264526126834 1106 7 0.22846538083950207 1106 8 0.3446874346216049 1106 9 0.2929378012705044 1106 10-11 0.39407753337695084 1106 12-13 0.3518568280993577 1106 14-15 0.3679047949492116 1106 16-17 0.39843995559046874 1106 18-19 0.4598422590847946 1106 20-21 0.5219162304131331 1106 22-23 0.42658919447787014 1106 24-25 0.6354133906753532 1106 26-27 0.5889511809167942 1106 28-29 0.5360174973982907 1106 30-31 0.5947818755051486 1106 32-33 0.7123583774466979 1106 34-35 0.675685142264733 1106 36-37 0.5590390330189834 1106 38-39 0.5876450499353254 1106 40-41 0.5058761190431582 1106 42-43 0.4915797239647475 1106 44-45 0.4132119613890879 1106 46-47 0.4001051579785795 1106 48-49 0.3848744604471861 1106 50-51 0.3855134636706339 1106 52-53 0.336200599859346 1106 54-55 0.3270973960782584 1106 56-57 0.44057646601549294 1106 58-59 0.4281927217391299 1106 60-61 0.4005814196027586 1106 62-63 0.4564694538657932 1106 64-65 0.5101538067177174 1106 66-67 0.3487180500532574 1106 68-69 0.3819498608042764 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26-27 -4.006872748111633 2216 28-29 -2.415500310780004 2216 30-31 -5.204814190168918 2216 32-33 -8.286702342341698 2216 34-35 -8.479104711158683 2216 36-37 -7.8825222743441685 2216 38-39 -7.572789438277741 2216 40-41 -7.537800643960896 2216 42-43 -7.956436511751594 2216 44-45 -6.1355794999373 2216 46-47 -3.1245131982297565 2216 48-49 -2.359121178442429 2216 50-51 -2.363038397728886 2216 52-53 -2.3875574722985675 2216 54-55 -2.4491588947022223 2216 56-57 -2.29106014418182 2216 58-59 -2.281488458334813 2216 60-61 -2.299213861892177 2216 62-63 -2.233445804797668 2216 64-65 -2.245876575560281 2216 66-67 -2.2182027038331213 2216 68-69 -2.453287836499591 2216 70-71 -2.5459128843810674 2216 72-73 -2.3702600337754163 2216 74-75 -2.535990228296143 2216 76-77 -2.4535224231207593 2216 78-79 -2.4359693323052163 2216 80-81 -2.4974099691973066 2216 82-83 -2.5135648543982505 2216 84-85 -2.4781658927043893 2216 86-87 -2.5096500149375345 2216 88-89 -2.3128554716228606 2216 90-91 -2.4356519472791085 2216 92-93 -2.461505679150708 2216 94-95 -2.367340063257757 2216 96-97 -2.479109980902809 2216 98-99 -2.443321948223442 2216 100-101 -2.4216266380745317 2216 102-103 -2.5019690477292365 2216 104-105 -2.4247032929685837 2216 106-107 -2.4275774390548612 2216 108-109 -2.40536475868905 2216 110-111 -2.4819667389448163 2216 112-113 -2.4488481862213227 2216 114-115 -2.538822730049393 2216 116-117 -2.489457328896087 2216 118-119 -2.372737663717899 2216 120-121 -2.1729007710120065 2216 122-123 -2.2070621047502748 2216 124-125 -2.05797789040464 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 29845.0 3 5968.0 4 4450.0 5 3933.0 6 4615.0 7 5099.0 8 4988.0 9 4989.0 10 5794.0 11 6793.0 12 7264.0 13 7033.0 14 7004.0 15 6718.0 16 6751.0 17 6047.0 18 5496.0 19 5132.0 20 5187.0 21 5389.0 22 6357.0 23 8689.0 24 13735.0 25 20143.0 26 23839.0 27 21538.0 28 24299.0 29 29956.0 30 35734.0 31 43485.0 32 56877.0 33 75880.0 34 109681.0 35 172334.0 36 341377.0 37 626278.0 38 253840.0 39 789.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.98791513472664 18.78246293500146 12.479902262184886 23.749719668087018 2 16.001705996307336 20.91653353136741 39.25274764788755 23.829012824437697 3 19.24192674455478 26.113176641555697 28.48987113254047 26.155025481349053 4 12.652051237234389 17.12407301056206 36.906040100876425 33.31783565132713 5 13.579508937199153 39.29920653112426 33.49518320596582 13.62610132571076 6 31.457286381990745 36.98523939004721 17.806080858131114 13.751393369830927 7 28.54099340994608 33.231801976916344 20.854360906534247 17.372843706603334 8 26.094504838453652 35.639631293159475 19.887227540599984 18.37863632778689 9 26.695279269889426 17.331190882303467 19.32928104594739 36.64424880185972 10-11 24.512083048589275 27.753997107715872 27.269362304762083 20.46455753893277 12-13 25.124795211058654 25.37323751723422 27.143981725987654 22.357985545719476 14-15 22.695681952741 26.66296088108172 24.638473742171175 26.0028834240061 16-17 22.64450784094965 29.587997593927913 25.373303082717463 22.394191482404974 18-19 22.63340836219923 28.520428767754456 26.86307955805352 21.983083311992786 20-21 23.596214054346028 27.66077152158942 26.67217633466965 22.070838089394904 22-23 23.326479500533072 27.45785042602017 26.507009057094166 22.708661016352593 24-25 22.906510294729884 27.700725518130966 26.914131879168835 22.47863230797032 26-27 22.667015476262392 27.843867433049276 26.741497641291602 22.74761944939673 28-29 22.632124393716417 27.901275367064898 26.745966928715625 22.720633310503057 30-31 22.996011867054246 27.637426848037112 26.578267795464537 22.788293489444104 32-33 22.63379518503811 27.68569391194875 26.72898623829511 22.951524664718026 34-35 23.142266165679303 27.782756475607822 26.559178383387138 22.51579897532574 36-37 23.258698550514087 27.482294864181373 26.50319854439832 22.755808040906224 38-39 22.769645432636796 27.826651184516788 26.551620744915773 22.85208263793065 40-41 22.937412515500107 27.591787589131854 26.80430229052142 22.666497604846615 42-43 22.89796987014647 27.54281632440147 26.564103997637893 22.995109807814174 44-45 22.654409698286297 27.740359392868747 26.667866340075964 22.937364568768988 46-47 23.006922860164313 27.759853101684456 26.35800948489498 22.875214553256253 48-49 22.722191258935084 27.769792217069256 26.532888626761714 22.975127897233946 50-51 22.62363649433033 27.641252498387654 26.67953548459236 23.055575522689654 52-53 22.972399256725474 27.576907573637964 26.494392370579007 22.956300799057555 54-55 22.630532733896626 27.581276888040918 26.675557379291483 23.112632998770973 56-57 22.53334038844373 27.92830343896697 26.391321016175507 23.147035156413796 58-59 22.66405714131673 27.467309380013916 26.665082514050205 23.20355096461915 60-61 22.667810599131716 27.610692190638993 26.729852543543984 22.991644666685303 62-63 22.459862213568968 27.538764914382707 26.89695971244571 23.104413159602615 64-65 22.36743944001907 27.46956557549921 26.746751893454867 23.416243091026853 66-67 22.429978854382775 27.74758243096725 26.377260574306177 23.4451781403438 68-69 22.61485924698591 27.369952072662045 26.708589254901 23.306599425451036 70-71 22.512249283113373 27.11489978562663 26.75888374094392 23.613967190316085 72-73 22.43859655253144 27.545030193179443 26.56268485429052 23.4536883999986 74-75 22.25233083870259 27.71303884460784 26.56720590125555 23.467424415434017 76-77 22.39830501283121 27.379763282832144 26.728618857006797 23.493312847329857 78-79 22.509657964057748 27.518021793378722 26.62355879315259 23.348761449410937 80-81 22.47588198119678 27.504150705295434 26.55695127076975 23.46301604273804 82-83 22.628840698897694 27.528637159483303 26.604735442439274 23.237786699179726 84-85 22.53566162622557 27.49739965358668 26.443457473509163 23.523481246678582 86-87 22.264082845095757 27.43643509899244 26.72228078105699 23.577201274854815 88-89 22.559140214347398 27.226961648088892 26.681334205232393 23.532563932331314 90-91 22.426606393025935 27.434822803083698 26.725995203932694 23.41257559995767 92-93 22.544834562691264 27.1837233059857 26.74776360686204 23.52367852446099 94-95 22.305356192651622 26.929815716463523 26.989741060616197 23.77508703026866 96-97 22.437645198358304 26.866151448564214 26.86632616081314 23.82987719226434 98-99 21.924824906414962 26.973486630975994 26.908179937913708 24.193508524695343 100-101 21.990809559077 27.015273385538475 26.96240426208008 24.031512793304444 102-103 22.02096735787429 26.87974644682607 26.934683300644117 24.164602894655527 104-105 21.932443820505192 26.98740017693071 26.865286034655256 24.214869967908843 106-107 21.907611646849546 27.054994850669413 26.81262054114783 24.22477296133321 108-109 21.922600306244313 27.141407750452068 26.584427464149822 24.351564479153797 110-111 21.8008503976942 27.380299885488828 26.589354154998407 24.229495561818563 112-113 21.761641087701378 27.4114796059736 26.87344016239535 23.95343914392967 114-115 21.76286249134295 27.54115313154272 26.603495375999337 24.092489001114995 116-117 21.529810262576017 27.463870983046363 26.90890431225958 24.097414442118037 118-119 21.508509806445826 27.433402903199873 26.732035458340857 24.326051832013444 120-121 21.38508968497814 27.604442569829946 26.77430929673304 24.236158448458873 122-123 21.531750711321177 27.576195865824783 26.652539263081742 24.239514159772295 124-125 21.582068805281374 27.52256321304961 26.555389533393154 24.33997844827586 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2796.0 1 2070.5 2 1218.5 3 916.5 4 637.5 5 508.0 6 461.0 7 470.5 8 513.0 9 514.0 10 461.0 11 406.0 12 405.5 13 430.5 14 449.0 15 471.5 16 531.5 17 612.0 18 742.0 19 896.5 20 1150.0 21 1552.0 22 2073.0 23 2824.5 24 3650.5 25 4808.0 26 7238.5 27 10419.5 28 12431.0 29 15102.0 30 19508.5 31 24148.5 32 30684.5 33 36726.5 34 41888.0 35 47403.0 36 54235.5 37 59861.0 38 61280.0 39 62232.0 40 63171.0 41 63697.0 42 63575.5 43 64510.0 44 67138.0 45 70966.5 46 72494.0 47 71988.5 48 72077.0 49 71987.5 50 71303.5 51 69187.5 52 67810.0 53 68273.0 54 68594.5 55 65253.0 56 59706.5 57 55251.0 58 52549.0 59 47279.0 60 39790.5 61 33481.5 62 27789.0 63 21798.5 64 15621.5 65 11461.5 66 8405.5 67 6346.0 68 4890.0 69 3599.0 70 2751.5 71 1945.5 72 1462.0 73 1255.0 74 880.5 75 573.0 76 400.0 77 236.0 78 155.0 79 88.5 80 47.5 81 39.0 82 35.0 83 25.0 84 19.0 85 13.5 86 10.0 87 8.5 88 11.0 89 11.0 90 7.5 91 5.5 92 3.0 93 4.0 94 5.0 95 2.5 96 1.0 97 2.0 98 1.5 99 0.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06164748024036028 2 0.0487688973237506 3 0.04397686647105863 4 0.04372728153081425 5 0.04257919080569014 6 0.00444261193634985 7 1.996679521954989E-4 8 0.0 9 6.489208446353714E-4 10-11 0.0032446042231768567 12-13 0.0021963474741504875 14-15 0.007587382183428958 16-17 0.003044936270981358 18-19 0.004841947840740848 20-21 0.009010016342821886 22-23 0.015499224789175602 24-25 0.0033943551873234813 26-27 0.016123187139786534 28-29 0.006888544350744712 30-31 0.006638959410500338 32-33 0.0024708909084192984 34-35 0.017371111841008403 36-37 0.015249639848931228 38-39 0.029426064454811652 40-41 0.005690536637571718 42-43 0.001522468135490679 44-45 1.4975096414662417E-4 46-47 0.0017720530757350526 48-49 0.0028702268128102965 50-51 0.0016971775936617407 52-53 0.0014226341593929295 54-55 0.005590702661473969 56-57 0.002495849402443736 58-59 0.007587382183428958 60-61 0.014675594486369167 62-63 0.011281239299045688 64-65 0.0021713889801260504 66-67 0.0036938571156167293 68-69 0.009459269235261759 70-71 0.0022712229562238 72-73 0.0029451022948836086 74-75 0.0011730492191485559 76-77 3.4941891634212305E-4 78-79 0.002845268318785859 80-81 0.0025956833785414856 82-83 0.0014226341593929295 84-85 0.011356114781119 86-87 0.00586524609574278 88-89 0.0017970115697594899 90-91 8.985057848797449E-4 92-93 0.002221305968174925 94-95 0.0 96-97 0.0015973436175639912 98-99 0.023386108900897807 100-101 0.013302877315025113 102-103 0.005840287601718342 104-105 0.014076590629782672 106-107 0.03187199686920651 108-109 0.0011730492191485559 110-111 0.0013976756653684922 112-113 0.015624017259297787 114-115 0.03109828355444895 116-117 0.047595848104602045 118-119 0.34999296170468513 120-121 0.11590724624948712 122-123 0.018119866661741525 124-125 0.3129795150664445 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2003326.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.85459650053037 #Duplication Level Percentage of deduplicated Percentage of total 1 84.07657675908943 46.1198669326572 2 8.785086108714916 9.638047074319424 3 2.759661807997529 4.541404048668857 4 1.2406520515488346 2.7222187074106614 5 0.6847180103074512 1.8779965086030619 6 0.43397800421226373 1.4283412986701518 7 0.2839571458035866 1.0903448259548612 8 0.21893111618906005 0.9607502431969299 9 0.1535560710389745 0.7580930685344753 >10 1.0828867007553902 12.241221172973784 >50 0.17156672665352435 6.4821915133180115 >100 0.10431192182775888 10.366335618425294 >500 0.0038888216467290264 1.2772833275347573 >1k 2.2875421451347216E-4 0.4959056597325239 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3952 0.19727193676915292 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 4.991698804887472E-5 0.0 0.0 0.0 0.0 3 4.991698804887472E-5 0.0 0.0 0.0 0.0 4 4.991698804887472E-5 0.0 0.0 0.0 4.991698804887472E-5 5 4.991698804887472E-5 0.0 0.0 0.0 4.991698804887472E-5 6 4.991698804887472E-5 0.0 0.0 0.0 9.983397609774944E-5 7 4.991698804887472E-5 0.0 0.0 0.0 9.983397609774944E-5 8 4.991698804887472E-5 0.0 0.0 0.0 9.983397609774944E-5 9 4.991698804887472E-5 0.0 0.0 0.0 9.983397609774944E-5 10-11 4.991698804887472E-5 0.0 0.0 0.0 9.983397609774944E-5 12-13 9.983397609774944E-5 0.0 0.0 0.0 9.983397609774944E-5 14-15 9.983397609774944E-5 0.0 0.0 7.487548207331209E-5 1.2479247012218682E-4 16-17 9.983397609774944E-5 0.0 0.0 9.983397609774944E-5 1.4975096414662417E-4 18-19 1.4975096414662417E-4 0.0 0.0 1.4975096414662417E-4 1.4975096414662417E-4 20-21 1.9966795219549888E-4 0.0 0.0 3.4941891634212305E-4 1.9966795219549888E-4 22-23 1.9966795219549888E-4 0.0 0.0 5.990038565864967E-4 2.4958494024437364E-4 24-25 2.9950192829324835E-4 0.0 0.0 0.0013727171713440547 3.4941891634212305E-4 26-27 2.9950192829324835E-4 0.0 0.0 0.003194687235127982 3.9933590439099776E-4 28-29 4.991698804887473E-4 0.0 0.0 0.00756242368940452 3.9933590439099776E-4 30-31 5.990038565864967E-4 0.0 0.0 0.019742168773329953 4.2429439841543517E-4 32-33 5.990038565864967E-4 0.0 0.0 0.042604149299714573 4.991698804887472E-4 34-35 7.237963267086834E-4 0.0 0.0 0.07817000328453781 5.49086868537622E-4 36-37 8.485887968308702E-4 0.0 0.0 0.12935987452865885 5.49086868537622E-4 38-39 9.234642789041824E-4 0.0 0.0 0.19896911436281464 5.740453625620594E-4 40-41 9.484227729286197E-4 0.0 0.0 0.2714236225157563 5.990038565864967E-4 42-43 0.0011480907251241188 0.0 0.0 0.3466235650113861 6.489208446353714E-4 44-45 0.0012978416892707428 0.0 0.0 0.4396189137464397 7.237963267086834E-4 46-47 0.001597343617563991 0.0 0.0 0.5523564312548233 8.985057848797449E-4 48-49 0.0016472606056128658 0.0 0.0 0.6670157528030884 0.001098173737075244 50-51 0.0016472606056128658 0.0 0.0 0.7918830984073486 0.001098173737075244 52-53 0.0016722190996373031 0.0 0.0 0.917798700760635 0.0011730492191485559 54-55 0.0017470945817106153 0.0 0.0 1.0541469536161365 0.0012978416892707428 56-57 0.00179701156975949 0.0 0.0 1.2162024553168083 0.0012978416892707428 58-59 0.00179701156975949 0.0 0.0 1.3997971373605693 0.00132280018329518 60-61 0.00179701156975949 0.0 0.0 1.604182244926687 0.0013477586773196174 62-63 0.00179701156975949 0.0 0.0 1.8118369152100058 0.001447592653417367 64-65 0.0018968455458572395 0.0 0.0 2.025856001469556 0.001447592653417367 66-67 0.0019966795219549887 0.0 0.0 2.2728202998413636 0.0014725511474418043 68-69 0.0019966795219549887 0.0 0.0 2.55667325238129 0.0014975096414662416 70-71 0.0020715550040283012 0.0 0.0 2.865684366897849 0.001522468135490679 72-73 0.0021464304861016133 0.0 0.0 3.1825324485380815 0.0017470945817106153 74-75 0.002196347474150488 0.0 0.0 3.5049712328397877 0.0017720530757350528 76-77 0.002196347474150488 0.0 0.0 3.857385168464843 0.00179701156975949 78-79 0.002196347474150488 0.0 0.0 4.257993956051087 0.00179701156975949 80-81 0.0025208078964681735 0.0 0.0 4.706997263550715 0.0018469285578083649 82-83 0.002545766390492611 0.0 0.0 5.179611306397462 0.001971721027930551 84-85 0.0025956833785414856 0.0 0.0 5.642691204526872 0.0019966795219549887 86-87 0.0026705588606147973 0.0 0.0 6.124839392090953 0.002021638015979426 88-89 0.002695517354639235 0.0 0.0 6.65106428010219 0.0020965134980527383 90-91 0.002845268318785859 0.0 0.0 7.232048104003043 0.002146430486101613 92-93 0.002895185306834734 0.0 0.0 7.832699221195153 0.002146430486101613 94-95 0.002895185306834734 0.0 0.0 8.450571699264124 0.002146430486101613 96-97 0.002895185306834734 0.0 0.0 9.072786955293347 0.0022462644621993625 98-99 0.0031447702470791075 0.0 0.0 9.712373323163579 0.002296181450248237 100-101 0.003194687235127982 0.0 0.0 10.428582267688833 0.002296181450248237 102-103 0.003269562717201294 0.0 0.0 11.18529884801575 0.0023211399442726747 104-105 0.00396840054988554 0.0 0.0 11.9437874814184 0.0023460984382971117 106-107 0.004242943984154352 0.0 0.0 12.69004645274908 0.0023960154263459868 108-109 0.004292860972203227 0.0 0.0 13.450606641155758 0.0024459324143948614 110-111 0.004342777960252101 0.0 0.0 14.269993999978038 0.0026705588606147977 112-113 0.004367736454276539 0.0 0.0 15.13445639900845 0.0027953513307369844 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2395 0.0 60.610847 1 GTATCAA 4490 0.0 45.7129 1 TCAACGC 5020 0.0 39.460552 4 ATCAACG 5170 0.0 38.431686 3 CAACGCA 5250 0.0 37.73275 5 AACGCAG 5300 0.0 37.255238 6 GTACATG 11035 0.0 36.283466 1 TATCAAC 5880 0.0 34.802834 2 TACATGG 11370 0.0 34.4793 2 ACATGGG 11420 0.0 32.817684 3 ACGCAGA 6355 0.0 30.788162 7 CGCAGAG 6380 0.0 30.38787 8 CATGGGA 6305 0.0 29.153858 4 CATGGGG 5075 0.0 26.490799 4 GTATAGA 555 0.0 25.726711 1 GCAGAGT 7685 0.0 24.300863 9 GTATAGG 930 0.0 23.669266 1 TGGTATC 1470 0.0 23.471678 2 ATGGGAG 3200 0.0 23.05182 5 TACGTTG 130 0.0020549837 22.880217 5 >>END_MODULE