FastQCFastQC Report
Thu 26 May 2016
SRR1364201_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364201_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2003326
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86240.43048410493349554No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75590.377322512661444No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39050.19492583833085578No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24650.12304537554047618No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47300.074.139021
GTATCAA88150.051.8718381
ATCAACG111350.040.4578863
TCAACGC111700.040.2778364
TATCAAC114800.039.3476752
CAACGCA114100.039.320425
AACGCAG117800.038.537116
TGGTATC22100.037.9704742
GTACATG109500.033.547781
GTGGTAT25800.033.2303961
ACGCAGA136750.033.1073767
TACATGG111250.032.8998762
CGCAGAG138000.032.5919348
ACATGGG109100.032.1283233
GCAGAGT153450.029.0778169
CATGGGG48850.027.5322284
GAGTACT94700.027.20262112-13
CATGGGA59850.027.0459484
CAGAGTA150150.026.17067310-11
GTACTTT100100.025.5868814-15
Produced by FastQC (version 0.11.5)