FastQCFastQC Report
Thu 26 May 2016
SRR1364200_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364200_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2464836
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19300.047.1651841
GTATCAA30450.041.031691
ATCAACG31100.038.0651173
TCAACGC32450.036.4822584
CAACGCA32650.036.2602545
GTACATG76600.034.6412471
AACGCAG34600.034.547256
TACATGG77050.032.8912472
ACATGGG78200.030.9615143
TATCAAC41100.028.9488622
ACGCAGA43350.027.1624937
CGCAGAG45100.025.5810728
CATGGGG47500.024.6726594
GAGTACA58550.020.5263561
CATGGGA43650.019.0803834
GCAGAGT57800.018.9318189
AGTACAT59100.018.0180952
GTATTAC6650.017.8935181
CGTTCGG1503.0259653E-617.8542234-35
AGAGTAC48750.017.74968710-11
Produced by FastQC (version 0.11.5)