##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364200_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2464836 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.151787786286796 33.0 31.0 34.0 28.0 34.0 2 31.181670910356715 33.0 31.0 34.0 28.0 34.0 3 31.29138328067263 34.0 31.0 34.0 28.0 34.0 4 34.702432129358705 37.0 35.0 37.0 32.0 37.0 5 34.77791301327959 37.0 35.0 37.0 32.0 37.0 6 34.84559013256866 37.0 35.0 37.0 32.0 37.0 7 34.7420019019521 37.0 35.0 37.0 32.0 37.0 8 34.72725974466455 37.0 35.0 37.0 32.0 37.0 9 36.253144225417024 39.0 37.0 39.0 32.0 39.0 10-11 36.199099453269916 39.0 37.0 39.0 32.0 39.0 12-13 36.16433507138 39.0 37.0 39.0 32.0 39.0 14-15 37.49081541327699 40.0 38.0 41.0 31.5 41.0 16-17 37.523571750818306 40.0 38.0 41.0 32.0 41.0 18-19 37.42699696856099 40.0 38.0 41.0 31.0 41.0 20-21 37.39295028147917 40.0 38.0 41.0 31.0 41.0 22-23 37.42487126932583 40.0 38.0 41.0 31.0 41.0 24-25 37.28419537851606 40.0 38.0 41.0 30.0 41.0 26-27 37.283316009665555 40.0 37.5 41.0 30.0 41.0 28-29 37.36212429549065 40.0 38.0 41.0 31.0 41.0 30-31 37.26258014732015 40.0 37.0 41.0 30.0 41.0 32-33 37.07354546103676 40.0 37.0 41.0 30.0 41.0 34-35 37.033305055589906 40.0 37.0 41.0 30.0 41.0 36-37 37.06495726287672 40.0 37.0 41.0 30.0 41.0 38-39 36.91286560241736 40.0 37.0 41.0 30.0 41.0 40-41 36.92037462127298 40.0 37.0 41.0 30.0 41.0 42-43 36.862916031735985 40.0 37.0 41.0 30.0 41.0 44-45 36.866376099667484 40.0 37.0 41.0 30.0 41.0 46-47 36.79975949718359 40.0 37.0 41.0 30.0 41.0 48-49 36.72315379197642 40.0 36.0 41.0 30.0 41.0 50-51 36.616815074106356 40.0 36.0 41.0 30.0 41.0 52-53 36.48136427737991 40.0 36.0 41.0 30.0 41.0 54-55 36.311509771846886 39.5 35.0 41.0 29.5 41.0 56-57 36.12967901312704 39.0 35.0 41.0 29.0 41.0 58-59 35.91014473173875 39.0 35.0 41.0 29.0 41.0 60-61 35.67908270570537 39.0 35.0 41.0 28.5 41.0 62-63 35.39698523552886 38.0 35.0 40.0 28.0 41.0 64-65 35.09789596549223 38.0 34.0 40.0 28.0 41.0 66-67 34.80130726750177 37.0 34.0 40.0 28.0 41.0 68-69 34.57663288754303 37.0 34.0 40.0 28.0 41.0 70-71 34.415638403528675 36.0 34.0 39.0 28.0 41.0 72-73 34.100584582503664 36.0 34.0 39.0 28.0 41.0 74-75 33.767687992223415 35.5 34.0 38.5 28.0 40.0 76-77 33.42754812084861 35.0 34.0 37.0 27.0 39.5 78-79 33.07321399882183 35.0 33.5 37.0 27.0 39.0 80-81 32.7611845169415 35.0 33.0 36.5 26.0 39.0 82-83 32.476761537075895 35.0 33.0 36.0 26.0 37.5 84-85 32.21219058793364 35.0 33.0 36.0 26.0 37.0 86-87 31.987443586510423 35.0 33.0 35.0 26.0 37.0 88-89 31.802203270318998 35.0 33.0 35.0 25.5 36.0 90-91 31.63910235812849 35.0 33.0 35.0 25.0 36.0 92-93 31.497130640740398 35.0 33.0 35.0 25.0 36.0 94-95 31.359932263241856 35.0 33.0 35.0 24.5 36.0 96-97 31.257427877554534 35.0 33.0 35.0 24.0 35.0 98-99 31.17314681382453 35.0 33.0 35.0 24.0 35.0 100-101 31.064694365061207 35.0 32.0 35.0 23.5 35.0 102-103 30.09123385896668 34.0 30.5 35.0 20.0 35.0 104-105 30.76184135577377 34.5 32.0 35.0 20.0 35.0 106-107 30.85581028514676 35.0 32.0 35.0 20.0 35.0 108-109 30.814647911666334 35.0 32.0 35.0 20.0 35.0 110-111 30.731900824233335 35.0 32.0 35.0 19.5 35.0 112-113 30.859782760394605 35.0 33.0 35.0 20.0 35.0 114-115 30.832740393275657 35.0 33.0 35.0 19.5 35.0 116-117 30.730954513809436 35.0 33.0 35.0 18.5 35.0 118-119 30.478342575327527 35.0 32.5 35.0 10.0 35.0 120-121 30.400138792195506 35.0 32.0 35.0 3.5 35.0 122-123 30.250252349446374 35.0 32.0 35.0 2.0 35.0 124-125 29.372470622791944 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.45427309986881426 1101 2 0.4311291486512836 1101 3 0.49593749307459234 1101 4 0.3665091847431654 1101 5 0.3294275541967835 1101 6 0.3578885191705865 1101 7 0.30853252646799945 1101 8 0.33465468757978556 1101 9 0.3928450796783238 1101 10-11 0.42596333262267194 1101 12-13 0.38318115699237865 1101 14-15 0.42891065080215185 1101 16-17 0.52739408081748 1101 18-19 0.6367376322510196 1101 20-21 0.7566461519602399 1101 22-23 0.6513873137221822 1101 24-25 0.8476026179764702 1101 26-27 0.8186033101386698 1101 28-29 0.728961155980663 1101 30-31 0.7900779252742183 1101 32-33 0.9485720026627789 1101 34-35 0.8662635404629668 1101 36-37 0.8074654197350952 1101 38-39 0.8389045902203165 1101 40-41 0.7569555630043823 1101 42-43 0.7322239908463288 1101 44-45 0.627028183524267 1101 46-47 0.5800813777464526 1101 48-49 0.4849359591461777 1101 50-51 0.4661621910480136 1101 52-53 0.3931281131059592 1101 54-55 0.44026973387954627 1101 56-57 0.48440014289008815 1101 58-59 0.532423362441584 1101 60-61 0.3673849362431767 1101 62-63 0.31247713400961885 1101 64-65 0.3331588166725865 1101 66-67 0.3404870891153422 1101 68-69 0.279118709486319 1101 70-71 0.3202476441349873 1101 72-73 0.3526712721509213 1101 74-75 0.3280663523317173 1101 76-77 0.3398491822081482 1101 78-79 0.3739510895119764 1101 80-81 0.4095052894268463 1101 82-83 0.42111620384796566 1101 84-85 0.35955892955822577 1101 86-87 0.33299977720965757 1101 88-89 0.3665373033406354 1101 90-91 0.35445505679609113 1101 92-93 0.37941875050424656 1101 94-95 0.3603404517440083 1101 96-97 0.34463029427699254 1101 98-99 0.2752200402715381 1101 100-101 0.2744488401497662 1101 102-103 0.24853359874310854 1101 104-105 0.2912647211042838 1101 106-107 0.28266204922011084 1101 108-109 0.214079360567478 1101 110-111 0.13556027212144173 1101 112-113 0.158225887573753 1101 114-115 0.10700921691350018 1101 116-117 0.10365619989176622 1101 118-119 0.018310863503419483 1101 120-121 -0.015556463674297305 1101 122-123 0.08860119558299218 1101 124-125 -0.029398538994584555 1102 1 0.0909792930728166 1102 2 0.18281620259600118 1102 3 0.11975655531328755 1102 4 0.22232691338466282 1102 5 0.1564383352960732 1102 6 0.14936330295133615 1102 7 0.15722406793164367 1102 8 0.1728842659812173 1102 9 0.18887736057525473 1102 10-11 0.25039628638000977 1102 12-13 0.2934403615765291 1102 14-15 0.36741942883131173 1102 16-17 0.2683820481336525 1102 18-19 0.34192818688838855 1102 20-21 0.4790622628128318 1102 22-23 0.42823554037665446 1102 24-25 0.5827545375184826 1102 26-27 0.601239446700454 1102 28-29 0.42075935491129 1102 30-31 0.5112415601467148 1102 32-33 0.5758518655233473 1102 34-35 0.5411755997117709 1102 36-37 0.4099212978143498 1102 38-39 0.4979581195282563 1102 40-41 0.4216581601314431 1102 42-43 0.3667004648010632 1102 44-45 0.2288005484663529 1102 46-47 0.21017886584124312 1102 48-49 0.21119151060109687 1102 50-51 0.13125100541235213 1102 52-53 0.13442095357885364 1102 54-55 0.08455964685698092 1102 56-57 0.09061188888617266 1102 58-59 0.09713424997905662 1102 60-61 0.13656297325228195 1102 62-63 0.11014476521649641 1102 64-65 0.15512002468344832 1102 66-67 0.15590511300241872 1102 68-69 0.1380050042790515 1102 70-71 0.1674957181745924 1102 72-73 0.1696593482230142 1102 74-75 0.1062515190937674 1102 76-77 0.06736211257404534 1102 78-79 0.22149222510208233 1102 80-81 0.2010335597263193 1102 82-83 0.14238302312949713 1102 84-85 0.1365841074454721 1102 86-87 0.11704684356324435 1102 88-89 0.11115589097562051 1102 90-91 0.029388287791888956 1102 92-93 0.07644949098944309 1102 94-95 0.055809163021997676 1102 96-97 0.01659390918118575 1102 98-99 -0.030616988088798536 1102 100-101 -0.03548280407930804 1102 102-103 -0.10770711911728625 1102 104-105 -0.026102217808766426 1102 106-107 -0.07156022113997906 1102 108-109 -0.06839341925170217 1102 110-111 -0.03807457363235045 1102 112-113 0.09205303519119568 1102 114-115 0.06936250784985276 1102 116-117 0.1035891188828657 1102 118-119 -0.1391249214736412 1102 120-121 -0.12249428916204863 1102 122-123 -0.1615672869509872 1102 124-125 -0.2720245309466449 1103 1 0.1194520571141453 1103 2 -0.18187606553360425 1103 3 0.16010200945585495 1103 4 0.14039278537035216 1103 5 0.0864946883704718 1103 6 0.06586844435300065 1103 7 0.1806286123312617 1103 8 0.19352188842261597 1103 9 0.22197866898821417 1103 10-11 0.24018433257739957 1103 12-13 0.17957073520720712 1103 14-15 0.2601641745952108 1103 16-17 0.20633229252079133 1103 18-19 0.38190000451994877 1103 20-21 0.46141402402133735 1103 22-23 0.3601110936152665 1103 24-25 0.5457853696010204 1103 26-27 0.49360516205911154 1103 28-29 0.4178791611742696 1103 30-31 0.43502670938556065 1103 32-33 0.6476225847831074 1103 34-35 0.6139481254887471 1103 36-37 0.42169135814433645 1103 38-39 0.5681090669293312 1103 40-41 0.5033170537591829 1103 42-43 0.3954784940995282 1103 44-45 0.3735825826583934 1103 46-47 0.33746179512816354 1103 48-49 0.2846744268260508 1103 50-51 0.2468469694966018 1103 52-53 0.2185362557943762 1103 54-55 0.19626223977482482 1103 56-57 0.1897338795305359 1103 58-59 0.22296797108965194 1103 60-61 0.17297598694052851 1103 62-63 0.3043991599282734 1103 64-65 0.2968390558584062 1103 66-67 0.24404061578916014 1103 68-69 0.28555979166631573 1103 70-71 0.29794486337356574 1103 72-73 0.2598489042442864 1103 74-75 0.3046899721365648 1103 76-77 0.2985945678397144 1103 78-79 0.32453207810826257 1103 80-81 0.2674475588936218 1103 82-83 0.25140878670082856 1103 84-85 0.223587329787037 1103 86-87 0.22003931776877295 1103 88-89 0.2181506346985813 1103 90-91 0.22790458784868406 1103 92-93 0.1626293426660972 1103 94-95 0.19754481322241446 1103 96-97 0.22492009924919287 1103 98-99 0.16025179041866267 1103 100-101 0.15705560336321867 1103 102-103 0.11738110749858066 1103 104-105 0.09924372032647355 1103 106-107 0.1458303352883199 1103 108-109 0.10148465541378826 1103 110-111 0.13106730736143035 1103 112-113 0.1500388559552981 1103 114-115 0.1544194090605373 1103 116-117 0.09370006982933532 1103 118-119 0.02252192481844517 1103 120-121 -0.0037602448843045977 1103 122-123 0.015709607005636173 1103 124-125 0.03251167578703473 1104 1 0.2517725059961933 1104 2 0.32529338925436946 1104 3 0.3294822219421185 1104 4 0.3114190461497728 1104 5 0.33716104728329555 1104 6 0.3677290318603923 1104 7 0.401630657344235 1104 8 0.37616349394494364 1104 9 0.4201075898724156 1104 10-11 0.4897373049918414 1104 12-13 0.4255070800669358 1104 14-15 0.46924406620838965 1104 16-17 0.517539768897656 1104 18-19 0.6302658656201245 1104 20-21 0.6563649800053568 1104 22-23 0.6436504972609001 1104 24-25 0.8246367256272222 1104 26-27 0.8317151073099254 1104 28-29 0.6008577741722902 1104 30-31 0.6772281834281841 1104 32-33 0.829867919420046 1104 34-35 0.7734387210378131 1104 36-37 0.6925407360770208 1104 38-39 0.7023745749443506 1104 40-41 0.650737904498186 1104 42-43 0.5668959217386274 1104 44-45 0.4676964217137183 1104 46-47 0.47393394359406216 1104 48-49 0.44397688231611454 1104 50-51 0.4252970356787955 1104 52-53 0.4991353152925626 1104 54-55 0.46424528193294634 1104 56-57 0.43177840478242047 1104 58-59 0.45296118273544295 1104 60-61 0.37213989081698173 1104 62-63 0.402422682681447 1104 64-65 0.4423640446011916 1104 66-67 0.4697549525437097 1104 68-69 0.4528900289354567 1104 70-71 0.47453394506859325 1104 72-73 0.4577404879327318 1104 74-75 0.41270887858203764 1104 76-77 0.392384584819105 1104 78-79 0.43882936151892693 1104 80-81 0.31926135949861134 1104 82-83 0.36688963696345667 1104 84-85 0.37338452983195225 1104 86-87 0.3427106060362064 1104 88-89 0.3991352749838697 1104 90-91 0.36265497830665083 1104 92-93 0.32286654375450397 1104 94-95 0.45867116394611074 1104 96-97 0.4350990577330478 1104 98-99 0.3964072624446331 1104 100-101 0.383295938323414 1104 102-103 0.34062821493378337 1104 104-105 0.3339689309242466 1104 106-107 0.446566052239465 1104 108-109 0.43430654646665445 1104 110-111 0.4165451065509096 1104 112-113 0.5355435079911786 1104 114-115 0.4923970749782356 1104 116-117 0.42150101736335444 1104 118-119 0.2530158980983046 1104 120-121 0.2846943355250886 1104 122-123 0.35769506733771905 1104 124-125 0.22637604140767564 1105 1 0.26437427048166384 1105 2 0.22217034080482279 1105 3 0.29366027147034046 1105 4 0.24372196084360098 1105 5 0.16834072050149018 1105 6 0.18136831153247357 1105 7 0.11441295798802997 1105 8 0.20231143140448893 1105 9 0.1453403566174245 1105 10-11 0.18608811854000606 1105 12-13 0.23801147992614347 1105 14-15 0.2607187003104201 1105 16-17 0.3005551769207315 1105 18-19 0.4189495696770891 1105 20-21 0.48574241412101316 1105 22-23 0.35963986947195536 1105 24-25 0.6660594130044473 1105 26-27 0.6519929229359533 1105 28-29 0.5133660061669332 1105 30-31 0.46093465320825544 1105 32-33 0.6570337855962052 1105 34-35 0.6368622656779266 1105 36-37 0.5710364951804721 1105 38-39 0.5248446559017594 1105 40-41 0.5214129848311018 1105 42-43 0.445934992488354 1105 44-45 0.378034813465824 1105 46-47 0.24973571148588292 1105 48-49 0.2352467100687221 1105 50-51 0.20855784230073482 1105 52-53 0.2242921489835652 1105 54-55 0.2277538432073669 1105 56-57 0.1911810458333889 1105 58-59 0.17851712623557603 1105 60-61 0.16532422504731414 1105 62-63 0.2364923537422996 1105 64-65 0.26784462277107934 1105 66-67 0.13416988481426273 1105 68-69 0.20008689282936132 1105 70-71 0.21002618996191558 1105 72-73 0.24533927186841709 1105 74-75 0.2897424651201703 1105 76-77 0.31029186985050217 1105 78-79 0.36409931417076535 1105 80-81 0.264015591879307 1105 82-83 0.1754773105450056 1105 84-85 0.23927227948848895 1105 86-87 0.22540425661358654 1105 88-89 0.15516223050838818 1105 90-91 0.103594532757036 1105 92-93 0.14293884337639895 1105 94-95 0.22384443324020253 1105 96-97 0.1700955378456861 1105 98-99 0.18147059203098692 1105 100-101 0.17156335892724428 1105 102-103 0.144915380970275 1105 104-105 0.1280132247326513 1105 106-107 0.21010810921622536 1105 108-109 0.12427146113777354 1105 110-111 0.0840345468888195 1105 112-113 0.08582864725381967 1105 114-115 0.13399334389762885 1105 116-117 0.18775736167646784 1105 118-119 0.06772335004048813 1105 120-121 0.012962437397440851 1105 122-123 0.0619975619159554 1105 124-125 0.004221047141687251 1106 1 0.2468601555148453 1106 2 0.3200632761676303 1106 3 0.3447259101932225 1106 4 0.24538549085097117 1106 5 0.26062646466015593 1106 6 0.21982513018954108 1106 7 0.20617179841461564 1106 8 0.22241989378108684 1106 9 0.28706260268559447 1106 10-11 0.2958251069068538 1106 12-13 0.29564539326629813 1106 14-15 0.3854480946600347 1106 16-17 0.3440190943330421 1106 18-19 0.44435609723016256 1106 20-21 0.4465537082956317 1106 22-23 0.4692809696139264 1106 24-25 0.6304081159683221 1106 26-27 0.5893539744321998 1106 28-29 0.4861470948628508 1106 30-31 0.5357515438433964 1106 32-33 0.6504514785557731 1106 34-35 0.6093248733974193 1106 36-37 0.5345521949856717 1106 38-39 0.6077472488734443 1106 40-41 0.4257644117359405 1106 42-43 0.4818101204310423 1106 44-45 0.4401471333830642 1106 46-47 0.36923954151196625 1106 48-49 0.4109719937437859 1106 50-51 0.324645190007665 1106 52-53 0.3832116799154264 1106 54-55 0.36812938937129047 1106 56-57 0.3535928616839783 1106 58-59 0.29229462703349185 1106 60-61 0.31316777178354016 1106 62-63 0.3533276846323403 1106 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86-87 -2.195469610073836 2216 88-89 -2.1311316590074156 2216 90-91 -2.1759275303582584 2216 92-93 -2.1983064385411843 2216 94-95 -2.059925687516767 2216 96-97 -2.1705055880087585 2216 98-99 -2.2016112723735937 2216 100-101 -2.242816902302117 2216 102-103 -2.3612965987435928 2216 104-105 -2.4220467646313963 2216 106-107 -2.3662147373277413 2216 108-109 -2.3266137873154733 2216 110-111 -2.280003450698633 2216 112-113 -2.291342805591295 2216 114-115 -2.36851972405573 2216 116-117 -2.276656136903245 2216 118-119 -2.1336213845146794 2216 120-121 -1.9765724991111142 2216 122-123 -2.032872536790869 2216 124-125 -1.8166126104584777 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 35988.0 3 7055.0 4 4820.0 5 4040.0 6 4435.0 7 4958.0 8 5248.0 9 5697.0 10 6613.0 11 7458.0 12 8082.0 13 8088.0 14 7906.0 15 7710.0 16 7335.0 17 6909.0 18 6684.0 19 6295.0 20 6276.0 21 6498.0 22 7600.0 23 10312.0 24 15173.0 25 21805.0 26 26183.0 27 26304.0 28 30234.0 29 36927.0 30 44042.0 31 53891.0 32 68955.0 33 92111.0 34 129333.0 35 199130.0 36 394342.0 37 780289.0 38 368653.0 39 1457.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.76583524372109 18.32811199019846 12.228700918731759 24.677351847348685 2 15.910170263444382 20.296282585523425 39.34495700184365 24.448590149188544 3 19.171308751256245 24.341800841988366 29.165333978435616 27.321556428319777 4 12.472967795146218 16.46581927875155 37.35383938486716 33.70737354123507 5 13.608720764337312 37.97645763799509 34.780083361441136 13.634738236226468 6 32.45627274608372 36.71758543601478 17.47763428557518 13.348507532326318 7 28.433945614800237 32.10649843295163 21.360421125232016 18.099134827016115 8 25.54754149971844 36.065523223451784 20.05330172068243 18.333633556147348 9 25.8172330454546 16.26384335350662 19.673557120606812 38.24536648043197 10-11 24.380069727067983 26.83272928362396 28.403900918266455 20.383300071041603 12-13 24.954777810302726 24.32492303048413 28.04345793093851 22.676841228274636 14-15 22.112185510859227 26.16341912301715 25.246284232291067 26.478111133832556 16-17 22.650519848196048 29.017250375087535 26.01222919248056 22.320000584235856 18-19 22.46714784438697 28.088747732244258 27.316062516723527 22.128041906645247 20-21 23.111108406164032 27.545869140070273 27.15189359820175 22.191128855563942 22-23 22.8753476991497 27.455623342659415 26.92673514809803 22.74229381009286 24-25 22.61216244561824 27.469813380743126 27.269002020893794 22.64902215274484 26-27 22.693270475801704 27.731293790925125 26.90703937536342 22.668396357909746 28-29 22.583816426688823 27.659157080536477 27.154526038353175 22.60250045442152 30-31 22.777219331940994 27.5258730604714 26.982333388445344 22.71457421914226 32-33 22.438969569021424 27.617922045201293 27.146540180450586 22.796568205326693 34-35 22.8372937897044 27.672803360353615 26.928438694708028 22.561464155233956 36-37 22.96889493763649 27.434566807145856 26.795877491760745 22.80066076345691 38-39 22.587930746218262 27.52514799862999 27.150971588483458 22.73594966666829 40-41 22.861815745942767 27.435181056472807 27.04196459327465 22.66103860430978 42-43 22.723393568696245 27.45717863513258 26.923829830653844 22.895597965517332 44-45 22.66864895227118 27.600061099511265 26.977564186436275 22.753725761781283 46-47 22.791699605785436 27.691174182465776 26.699623028394686 22.817503183354095 48-49 22.739196258296197 27.58206226611562 26.827039311543377 22.85170216404481 50-51 22.65548896883263 27.592666147081964 26.928369933491265 22.823474950594147 52-53 22.808370986843148 27.537458429673173 26.819225421626676 22.83494516185701 54-55 22.72271470150224 27.349251942490792 26.98253142847753 22.94550192752944 56-57 22.60341638506489 27.670118376204826 26.871260552205907 22.855204686524377 58-59 22.703641061752002 27.41928841492893 26.841370764593147 23.035699758725926 60-61 22.654010186022834 27.362169858615825 27.094037607901623 22.889782347459715 62-63 22.600222355671693 27.499188482950025 27.03461525976661 22.86597390161167 64-65 22.420390202420347 27.417505554266274 26.98683691635575 23.175267326957623 66-67 22.47477021973055 27.597975612902914 26.747948875867937 23.179305291498597 68-69 22.488855203048928 27.398449161994854 27.03114862718255 23.081547007773665 70-71 22.375587273509197 27.21017697319842 27.135038421278978 23.279197332013403 72-73 22.432301231701825 27.295491793218858 27.029827035933106 23.24237993914622 74-75 22.36099762497292 27.45667624413039 26.938584412454652 23.243741718442042 76-77 22.496674214175332 27.26160535955609 26.98249900297424 23.259221423294335 78-79 22.47473692196775 27.307554519440615 27.09157026456499 23.126138294026646 80-81 22.422145974497447 27.406174929699407 27.012690418143983 23.15898867765916 82-83 22.38340066476049 27.350867566199653 27.06847114215265 23.197260626887196 84-85 22.494631875702968 27.243453972087377 26.96113039794721 23.300783754262444 86-87 22.402623287642054 27.062959349712244 27.174595948745754 23.359821413899944 88-89 22.446225707392063 27.02695520475756 27.18376350468376 23.34305558316662 90-91 22.49200093232017 27.17240640639463 27.09911500583106 23.236477655454145 92-93 22.355463530381662 26.994408638970263 27.1757632360963 23.474364594551776 94-95 22.33256086814701 26.864038824489743 27.226415875133274 23.576984432229974 96-97 22.35578976024583 26.86443966649566 27.166858264712022 23.61291230854649 98-99 22.312205674943193 26.757286336418623 27.118435317322714 23.81207267131547 100-101 22.143956053542905 26.79792903375941 27.272780760521552 23.785334152176134 102-103 22.16214439762508 26.672518692638192 27.310919681653573 23.854417228083154 104-105 22.16703634957118 26.77874351364243 27.27294121991098 23.781278916875415 106-107 22.007380963348222 26.791168087063465 27.270137292736347 23.931313656851966 108-109 22.04131145878236 26.794512767198313 27.063052463422675 24.10112331059665 110-111 21.953958074437775 26.953071591697004 27.167206873426085 23.925763460439136 112-113 21.91452811802512 26.895528753264706 27.297062661079696 23.892880467630476 114-115 21.91110414877608 26.94372927194718 27.156245079243458 23.98892150003328 116-117 21.752645340229407 26.856101650231196 27.32317770017306 24.068075309366343 118-119 21.769687938627964 26.925728803104835 27.096645485293653 24.207937772973544 120-121 21.774470667368544 26.96911576429913 27.16674221598331 24.089671352349015 122-123 21.845770974697835 26.953376394979006 27.113315592519644 24.087537037803518 124-125 21.76187344273878 26.95843531927467 27.038084067075886 24.241607170910665 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1100.0 1 843.5 2 539.5 3 439.0 4 355.0 5 298.5 6 279.0 7 270.0 8 268.0 9 284.0 10 271.0 11 254.5 12 260.5 13 285.5 14 336.0 15 404.0 16 488.0 17 598.0 18 766.0 19 1010.5 20 1378.5 21 1897.5 22 2661.0 23 3727.0 24 5233.5 25 7285.0 26 10205.5 27 14069.5 28 17931.0 29 22097.0 30 27325.0 31 32782.5 32 38973.5 33 44615.0 34 50023.5 35 55647.0 36 61275.5 37 67222.0 38 70804.0 39 73155.5 40 75877.5 41 79406.0 42 82402.5 43 85888.5 44 89606.0 45 92562.0 46 93587.0 47 93251.0 48 94164.0 49 93601.5 50 91559.0 51 89997.5 52 88598.5 53 86479.0 54 83517.0 55 78419.0 56 71459.5 57 65174.0 58 60316.5 59 53927.0 60 45455.5 61 36627.0 62 28637.5 63 22115.0 64 16824.5 65 12585.0 66 9250.5 67 7432.0 68 5738.5 69 3815.5 70 2655.0 71 1877.0 72 1378.0 73 1088.0 74 812.0 75 586.5 76 398.5 77 229.5 78 124.5 79 67.0 80 52.0 81 38.0 82 23.0 83 17.0 84 12.0 85 13.5 86 14.0 87 11.5 88 10.5 89 6.0 90 6.0 91 6.0 92 3.5 93 3.0 94 2.5 95 4.0 96 5.0 97 3.0 98 2.0 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06284393768997207 2 0.050388747973496004 3 0.04641282422035381 4 0.04495228080083219 5 0.04491171015028992 6 0.005111901968325682 7 4.462771559649405E-4 8 0.0 9 0.0010142662635566828 10-11 0.0033673639950081873 12-13 0.0024950950083494398 14-15 0.008276412710622532 16-17 0.0031645107422968504 18-19 0.004848192739800944 20-21 0.00876326051712974 22-23 0.01708024387829454 24-25 0.0034485052960927217 26-27 0.017323667781548143 28-29 0.00665358668893184 30-31 0.006430448110949369 32-33 0.0024950950083494398 34-35 0.01835821937037596 36-37 0.016796249324498667 38-39 0.025316085938374804 40-41 0.006613016038389572 42-43 0.0013185461426236877 44-45 1.825679274402029E-4 46-47 0.0024950950083494398 48-49 0.0031036547664834496 50-51 0.0019068205754865638 52-53 0.0015011140700638907 54-55 0.006187024207695766 56-57 0.00271823358633191 58-59 0.008580692589689536 60-61 0.015437132531332713 62-63 0.012820325571356471 64-65 0.0025965216347051084 66-67 0.0036513585488040586 68-69 0.009452961576348284 70-71 0.00227195643036697 72-73 0.003063084115941182 74-75 8.92554311929881E-4 76-77 2.0285325271133658E-4 78-79 0.002961657489585514 80-81 0.002555950984162841 82-83 0.0015619700458772917 84-85 0.011684347356172986 86-87 0.005781317702273092 88-89 0.0017445379733174945 90-91 7.099863844896781E-4 92-93 0.002089388502926767 94-95 0.0 96-97 0.0016431113469618263 98-99 0.025052376709850065 100-101 0.015518273832417249 102-103 0.006592730713118438 104-105 0.013652023907472952 106-107 0.03223338185583138 108-109 9.534102877432819E-4 110-111 0.0015822553711484252 112-113 0.016694822698143 114-115 0.03180739002513758 116-117 0.049151343131956855 118-119 0.3516866842256442 120-121 0.11635662575522265 122-123 0.0187436405505275 124-125 0.31636993292860055 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2464836.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.49688913215705 #Duplication Level Percentage of deduplicated Percentage of total 1 86.98085885736701 66.53765116631804 2 8.496424602597228 12.999001016892233 3 2.212877901751876 5.078348265399408 4 0.8684799054672343 2.6574404416813304 5 0.41876409471289927 1.6017075262890386 6 0.25780032692727683 1.1832553816313844 7 0.146809996817193 0.7861355635012 8 0.1129595990466074 0.6912846339744992 9 0.07609880182590449 0.5239189445729587 >10 0.39314198616727464 5.446405168013484 >50 0.026617128811866272 1.3651325674229913 >100 0.009058955583590003 1.0046546574989073 >500 0.0 0.0 >1k 1.0784292406778436E-4 0.1250646668044901 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 8.114130108453462E-5 0.0 2 0.0 0.0 0.0 8.114130108453462E-5 0.0 3 0.0 0.0 0.0 8.114130108453462E-5 0.0 4 0.0 0.0 0.0 8.114130108453462E-5 0.0 5 0.0 0.0 0.0 8.114130108453462E-5 0.0 6 0.0 0.0 0.0 8.114130108453462E-5 0.0 7 0.0 0.0 0.0 8.114130108453462E-5 0.0 8 0.0 0.0 0.0 8.114130108453462E-5 0.0 9 0.0 0.0 0.0 1.2171195162680195E-4 0.0 10-11 0.0 0.0 0.0 1.2171195162680195E-4 0.0 12-13 0.0 0.0 0.0 1.2171195162680195E-4 0.0 14-15 0.0 0.0 0.0 1.6228260216906925E-4 0.0 16-17 0.0 0.0 0.0 1.6228260216906925E-4 0.0 18-19 0.0 0.0 0.0 1.6228260216906925E-4 0.0 20-21 0.0 0.0 0.0 1.825679274402029E-4 0.0 22-23 0.0 0.0 0.0 4.0570650542267315E-4 4.057065054226731E-5 24-25 0.0 0.0 0.0 7.099863844896781E-4 4.057065054226731E-5 26-27 0.0 0.0 0.0 0.0010345515888278165 4.057065054226731E-5 28-29 0.0 0.0 0.0 0.003205081392839118 4.057065054226731E-5 30-31 0.0 0.0 0.0 0.010406371864091567 4.057065054226731E-5 32-33 0.0 0.0 0.0 0.026391208177744888 4.057065054226731E-5 34-35 0.0 0.0 0.0 0.05529779668911035 8.114130108453462E-5 36-37 0.0 0.0 0.0 0.09684214284439208 8.114130108453462E-5 38-39 0.0 0.0 0.0 0.14940142062189937 8.114130108453462E-5 40-41 0.0 0.0 0.0 0.20530777706914374 8.114130108453462E-5 42-43 0.0 0.0 0.0 0.25882046513439433 8.114130108453462E-5 44-45 0.0 0.0 0.0 0.33373417136069095 8.114130108453462E-5 46-47 0.0 0.0 0.0 0.4269655263068212 8.114130108453462E-5 48-49 0.0 0.0 0.0 0.5228339735381989 1.2171195162680195E-4 50-51 0.0 0.0 0.0 0.6212583717537394 1.2171195162680195E-4 52-53 0.0 0.0 0.0 0.7187293596815366 1.2171195162680195E-4 54-55 0.0 0.0 0.0 0.8315157681890397 1.2171195162680195E-4 56-57 0.0 0.0 0.0 0.9705513875973898 1.2171195162680195E-4 58-59 0.0 0.0 0.0 1.12461843303165 1.2171195162680195E-4 60-61 0.0 0.0 0.0 1.2864141873942119 1.2171195162680195E-4 62-63 0.0 0.0 0.0 1.4465871157350834 1.2171195162680195E-4 64-65 0.0 0.0 0.0 1.6213046222953575 1.2171195162680195E-4 66-67 0.0 0.0 0.0 1.8295740568540868 1.2171195162680195E-4 68-69 0.0 0.0 0.0 2.066344373418759 1.2171195162680195E-4 70-71 0.0 0.0 0.0 2.328613343849246 1.2171195162680195E-4 72-73 0.0 0.0 0.0 2.5943105342505546 1.6228260216906925E-4 74-75 0.0 0.0 0.0 2.8603322898562014 1.6228260216906925E-4 76-77 0.0 0.0 0.0 3.1622590711917544 1.6228260216906925E-4 78-79 0.0 0.0 0.0 3.5000705929319436 1.6228260216906925E-4 80-81 0.0 0.0 0.0 3.869425795468745 1.6228260216906925E-4 82-83 0.0 0.0 0.0 4.256043809811281 1.6228260216906925E-4 84-85 0.0 0.0 0.0 4.644588930054576 1.6228260216906925E-4 86-87 0.0 0.0 0.0 5.061269796448932 1.6228260216906925E-4 88-89 0.0 0.0 0.0 5.516391354232087 1.6228260216906925E-4 90-91 0.0 0.0 0.0 6.020745396448283 1.6228260216906925E-4 92-93 0.0 0.0 0.0 6.5417536907120795 1.6228260216906925E-4 94-95 0.0 0.0 0.0 7.057893506910805 1.6228260216906925E-4 96-97 0.0 0.0 0.0 7.598537996037059 1.6228260216906925E-4 98-99 0.0 0.0 0.0 8.169042483962421 1.6228260216906925E-4 100-101 0.0 2.0285325271133656E-5 0.0 8.796792159802925 1.6228260216906925E-4 102-103 0.0 4.057065054226731E-5 0.0 9.452758723095574 1.825679274402029E-4 104-105 0.0 4.057065054226731E-5 0.0 10.103451101817727 2.0285325271133658E-4 106-107 0.0 6.085597581340097E-5 0.0 10.750857257845958 2.6370922852473754E-4 108-109 0.0 8.114130108453462E-5 0.0 11.424816904654104 2.839945537958712E-4 110-111 0.0 8.114130108453462E-5 0.0 12.137764946633366 2.839945537958712E-4 112-113 0.0 8.114130108453462E-5 0.0 12.895502986811293 2.839945537958712E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1930 0.0 47.165184 1 GTATCAA 3045 0.0 41.03169 1 ATCAACG 3110 0.0 38.065117 3 TCAACGC 3245 0.0 36.482258 4 CAACGCA 3265 0.0 36.260254 5 GTACATG 7660 0.0 34.641247 1 AACGCAG 3460 0.0 34.54725 6 TACATGG 7705 0.0 32.891247 2 ACATGGG 7820 0.0 30.961514 3 TATCAAC 4110 0.0 28.948862 2 ACGCAGA 4335 0.0 27.162493 7 CGCAGAG 4510 0.0 25.581072 8 CATGGGG 4750 0.0 24.672659 4 GAGTACA 5855 0.0 20.526356 1 CATGGGA 4365 0.0 19.080383 4 GCAGAGT 5780 0.0 18.931818 9 AGTACAT 5910 0.0 18.018095 2 GTATTAC 665 0.0 17.893518 1 CGTTCGG 150 3.0259653E-6 17.85422 34-35 AGAGTAC 4875 0.0 17.749687 10-11 >>END_MODULE