FastQCFastQC Report
Thu 26 May 2016
SRR1364200_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364200_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2464836
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52030.21108909477141682No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50720.2057743395503798No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25540.10361744148495071No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36100.071.737311
GTATCAA63900.050.123851
ATCAACG78000.040.3588683
TCAACGC79950.039.3745044
CAACGCA80150.039.2666935
AACGCAG83750.038.0042656
TATCAAC85300.037.4645732
TGGTATC17950.032.490562
GTACATG72050.032.142421
ACGCAGA98750.032.110327
CGCAGAG101200.031.2741588
TACATGG75700.029.401622
GTGGTAT21200.028.92441
ACATGGG75650.028.633233
GCAGAGT115750.027.0859579
GAGTACT79900.025.94892512-13
CAGAGTA110400.025.43423510-11
AGAGTAC104850.024.34078410-11
GTACTTT85650.023.85957514-15
AGTACTT85600.022.622512-13
Produced by FastQC (version 0.11.5)