FastQCFastQC Report
Thu 26 May 2016
SRR1364199_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364199_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3062152
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25900.048.2371561
GTATCAA41550.045.1026151
TCAACGC45600.039.788884
ATCAACG45650.039.7452963
CAACGCA47250.038.3994265
AACGCAG48350.037.2669566
GTACATG114800.036.5868451
TATCAAC53350.034.677892
TACATGG121650.033.6925432
ACATGGG116900.033.3313943
ACGCAGA60500.029.5856957
CGCAGAG64250.027.4886868
CATGGGG75400.026.1934934
GAGTACA84550.023.3607431
AGTACAT82750.022.7886162
CATGGGA60700.022.7366264
GCAGAGT81550.021.2221479
AGAGTAC65000.020.35808610-11
ATGGGGA44350.019.8515665
TAGTGCG2751.3251265E-619.4639119
Produced by FastQC (version 0.11.5)