##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364199_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3062152 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.257009122995854 33.0 31.0 34.0 28.0 34.0 2 31.235057240790137 33.0 31.0 34.0 28.0 34.0 3 31.361504262361894 33.0 31.0 34.0 28.0 34.0 4 34.80701153959699 37.0 35.0 37.0 32.0 37.0 5 34.85210172453882 37.0 35.0 37.0 32.0 37.0 6 34.926274071306715 37.0 35.0 37.0 32.0 37.0 7 34.812965848854006 37.0 35.0 37.0 32.0 37.0 8 34.770884658893486 37.0 35.0 37.0 32.0 37.0 9 36.31349031661394 39.0 37.0 39.0 32.0 39.0 10-11 36.27678002267686 39.0 37.0 39.0 32.0 39.0 12-13 36.26914846160478 39.0 37.0 39.0 32.0 39.0 14-15 37.61514647215422 40.0 38.0 41.0 32.0 41.0 16-17 37.63389799069412 40.0 38.0 41.0 32.0 41.0 18-19 37.53082015523723 40.0 38.0 41.0 31.5 41.0 20-21 37.495270809548316 40.0 38.0 41.0 31.0 41.0 22-23 37.498794965109504 40.0 38.0 41.0 31.5 41.0 24-25 37.37415076064154 40.0 38.0 41.0 31.0 41.0 26-27 37.36750151527423 40.0 38.0 41.0 31.0 41.0 28-29 37.43745885246716 40.0 38.0 41.0 31.0 41.0 30-31 37.35261721168642 40.0 38.0 41.0 30.5 41.0 32-33 37.1914052600916 40.0 37.0 41.0 30.0 41.0 34-35 37.150769948715805 40.0 37.0 41.0 30.0 41.0 36-37 37.17230790633515 40.0 37.0 41.0 30.0 41.0 38-39 37.01998937348636 40.0 37.0 41.0 30.0 41.0 40-41 37.01706528611251 40.0 37.0 41.0 30.0 41.0 42-43 36.94750668810693 40.0 37.0 41.0 30.0 41.0 44-45 36.91473920954937 40.0 37.0 41.0 30.0 41.0 46-47 36.79946913151274 40.0 36.5 41.0 30.0 41.0 48-49 36.6915251104452 40.0 36.0 41.0 30.0 41.0 50-51 36.559828022906764 40.0 36.0 41.0 30.0 41.0 52-53 36.407119404915235 40.0 35.5 41.0 30.0 41.0 54-55 36.21693485496474 39.0 35.0 41.0 29.5 41.0 56-57 36.016423253973024 39.0 35.0 41.0 29.0 41.0 58-59 35.774526705401954 39.0 35.0 41.0 29.0 41.0 60-61 35.516245601132795 38.0 35.0 40.0 28.5 41.0 62-63 35.224372924662134 38.0 34.5 40.0 28.0 41.0 64-65 34.91741608515841 37.0 34.0 40.0 28.0 41.0 66-67 34.604979112728564 37.0 34.0 40.0 28.0 41.0 68-69 34.371463761433134 36.0 34.0 39.0 28.0 41.0 70-71 34.213288563075906 36.0 34.0 39.0 28.0 41.0 72-73 33.906050712048255 35.5 34.0 39.0 28.0 40.5 74-75 33.57874690740368 35.0 34.0 37.5 28.0 40.0 76-77 33.25686249408913 35.0 34.0 37.0 27.0 39.0 78-79 32.92823266121342 35.0 33.0 36.5 27.0 39.0 80-81 32.64629825691213 35.0 33.0 36.0 27.0 38.0 82-83 32.39158980351074 35.0 33.0 36.0 26.0 37.0 84-85 32.16074544960537 35.0 33.0 35.0 26.0 37.0 86-87 31.963957373768515 35.0 33.0 35.0 26.0 36.5 88-89 31.80757192980623 35.0 33.0 35.0 25.5 36.0 90-91 31.66949305586398 35.0 33.0 35.0 25.0 36.0 92-93 31.55465731289629 35.0 33.0 35.0 25.0 36.0 94-95 31.439559499332496 35.0 33.0 35.0 25.0 35.0 96-97 31.35105703439934 35.0 33.0 35.0 25.0 35.0 98-99 31.280896082232367 35.0 33.0 35.0 24.0 35.0 100-101 31.19045462145576 35.0 33.0 35.0 24.0 35.0 102-103 30.19519213938433 34.0 30.5 35.0 21.5 35.0 104-105 30.911788017054672 34.5 32.0 35.0 23.0 35.0 106-107 31.031911381276956 35.0 32.0 35.0 23.0 35.0 108-109 30.997576704226308 35.0 32.0 35.0 23.0 35.0 110-111 30.939864513583913 35.0 32.0 35.0 23.0 35.0 112-113 31.109533916017234 35.0 33.0 35.0 24.0 35.0 114-115 31.113246337869576 35.0 33.0 35.0 23.0 35.0 116-117 31.03045668536376 35.0 33.0 35.0 23.0 35.0 118-119 30.80095681076576 35.0 33.0 35.0 19.0 35.0 120-121 30.756706068150763 35.0 33.0 35.0 19.0 35.0 122-123 30.631055381966668 35.0 33.0 35.0 14.0 35.0 124-125 29.74767908320684 34.5 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.45284763310997533 1101 2 0.4902018034190263 1101 3 0.5187545418406394 1101 4 0.5200776008025016 1101 5 0.3825281219715819 1101 6 0.43099435324847235 1101 7 0.44596534892927053 1101 8 0.3615120053943883 1101 9 0.5272032294019198 1101 10-11 0.5399608950349872 1101 12-13 0.5557085170246552 1101 14-15 0.6360719113363942 1101 16-17 0.6314250982536862 1101 18-19 0.7206207509506939 1101 20-21 0.7886149290549866 1101 22-23 0.7406170689583149 1101 24-25 0.8860051100607294 1101 26-27 0.9079625989810438 1101 28-29 0.7510298753439315 1101 30-31 0.8721585364825373 1101 32-33 1.0207046394474872 1101 34-35 0.8836709486321297 1101 36-37 0.7908636142089591 1101 38-39 0.8321653106806934 1101 40-41 0.7550452489811974 1101 42-43 0.768232825888532 1101 44-45 0.6173236181906034 1101 46-47 0.5910990553690993 1101 48-49 0.49123153871217795 1101 50-51 0.5587860905191206 1101 52-53 0.4083256196036231 1101 54-55 0.45217482593735525 1101 56-57 0.4450084330054196 1101 58-59 0.5191217810128919 1101 60-61 0.39229833797669755 1101 62-63 0.33886024506563217 1101 64-65 0.31151442938769947 1101 66-67 0.2705424009666544 1101 68-69 0.2911180372278679 1101 70-71 0.22968818273542269 1101 72-73 0.22996969120026023 1101 74-75 0.2532736361457424 1101 76-77 0.2637212102402273 1101 78-79 0.2803700256307806 1101 80-81 0.23884358042950282 1101 82-83 0.3269312307016108 1101 84-85 0.2668642970041475 1101 86-87 0.32577654781491816 1101 88-89 0.3056730990535641 1101 90-91 0.3082179277825077 1101 92-93 0.3330058992803586 1101 94-95 0.31345271249338325 1101 96-97 0.3440498619587906 1101 98-99 0.33103851738465195 1101 100-101 0.24384605688887717 1101 102-103 0.08590865892161403 1101 104-105 0.27327679619722645 1101 106-107 0.3303806316418658 1101 108-109 0.26723837577713283 1101 110-111 0.26791141118305717 1101 112-113 0.4161714465319122 1101 114-115 0.36336929030876064 1101 116-117 0.43019144896186745 1101 118-119 0.29058577438582844 1101 120-121 0.26604501953028503 1101 122-123 0.2522137487123004 1101 124-125 0.038604856530138676 1102 1 0.03232801352875825 1102 2 -0.002569379295835006 1102 3 -0.015647449728007246 1102 4 0.029726711499691305 1102 5 0.006763764542569106 1102 6 0.06533347693245162 1102 7 0.006239762557733286 1102 8 0.0918143399400222 1102 9 0.07669082763622725 1102 10-11 0.13178897466965367 1102 12-13 0.09498610316550327 1102 14-15 0.13509171794756725 1102 16-17 0.11739994836705137 1102 18-19 0.1745138975974001 1102 20-21 0.25265245415702964 1102 22-23 0.18220126991919017 1102 24-25 0.42600301713451927 1102 26-27 0.4056392482900577 1102 28-29 0.22390685684490563 1102 30-31 0.29484950810680743 1102 32-33 0.4189038748085352 1102 34-35 0.3721075549009356 1102 36-37 0.24287220849332414 1102 38-39 0.27476567867196167 1102 40-41 0.18921072858051247 1102 42-43 0.1277105705961077 1102 44-45 -0.04240865593298082 1102 46-47 -0.05646362086817902 1102 48-49 -0.040141811477020894 1102 50-51 -0.1346589984144657 1102 52-53 -0.06843653874513933 1102 54-55 -0.10616846041758521 1102 56-57 -0.13948187185081196 1102 58-59 -0.0676750538532005 1102 60-61 -0.1675519541919357 1102 62-63 -0.0804471075556421 1102 64-65 -0.10150966852317111 1102 66-67 -0.10045477754077581 1102 68-69 -0.02783070035645352 1102 70-71 -0.0659677986210383 1102 72-73 -0.12987287853241725 1102 74-75 -0.13138450329939388 1102 76-77 -0.10358389943508683 1102 78-79 -0.04120735565113165 1102 80-81 0.01513855731708702 1102 82-83 -0.0196941943562976 1102 84-85 -0.05792378479412719 1102 86-87 -0.05128981043175784 1102 88-89 -0.06274014955567608 1102 90-91 -0.13399203078631672 1102 92-93 -0.07405884804450835 1102 94-95 -0.09726624535186801 1102 96-97 -0.06936987533421402 1102 98-99 -0.06719652670932419 1102 100-101 -0.0988093576059228 1102 102-103 -0.2065672478085112 1102 104-105 -0.06740916154479848 1102 106-107 -0.03227016729572796 1102 108-109 -0.09969644332963412 1102 110-111 -0.10036185355048133 1102 112-113 -0.06644805463076509 1102 114-115 -0.05486158583716971 1102 116-117 -0.060713768950055425 1102 118-119 -0.25574238636032476 1102 120-121 -0.17760313083322643 1102 122-123 -0.22270016073297327 1102 124-125 -0.3323248743441489 1103 1 0.05580537138918373 1103 2 -0.27762913644502873 1103 3 0.046719013009436594 1103 4 0.044829028198762444 1103 5 0.07574283654203384 1103 6 -0.02275265954946093 1103 7 -0.03797340804441518 1103 8 0.030631731553924624 1103 9 0.0836714182143865 1103 10-11 0.051766786183215174 1103 12-13 0.042741468661922966 1103 14-15 0.08970052467352474 1103 16-17 0.059996760101235225 1103 18-19 0.17593952073226404 1103 20-21 0.23934866019284584 1103 22-23 0.1928376209085343 1103 24-25 0.43711103154836906 1103 26-27 0.3810735678876327 1103 28-29 0.2500271599423485 1103 30-31 0.2894692334229916 1103 32-33 0.5246979411098138 1103 34-35 0.40040155211202944 1103 36-37 0.28734227745702867 1103 38-39 0.38917643781611133 1103 40-41 0.33553037674989383 1103 42-43 0.27621374944692434 1103 44-45 0.18159990000734894 1103 46-47 0.17968684301967386 1103 48-49 0.07784203406826151 1103 50-51 0.07477581582195114 1103 52-53 0.0020014601971993784 1103 54-55 -0.018082628400875933 1103 56-57 0.023574438752397953 1103 58-59 -0.0023343643285329563 1103 60-61 -0.07252677492049031 1103 62-63 -0.09750514471069494 1103 64-65 -0.05998003071028535 1103 66-67 0.02114175830110554 1103 68-69 -0.010250786440479942 1103 70-71 0.024161533508795685 1103 72-73 0.05845561224298734 1103 74-75 0.05371199577245278 1103 76-77 0.06911831346477015 1103 78-79 0.07667529034812048 1103 80-81 0.08956481608158384 1103 82-83 0.05571375387150823 1103 84-85 0.039561660598636195 1103 86-87 0.03743261146237842 1103 88-89 0.05264848152665991 1103 90-91 0.08241981412754384 1103 92-93 0.09525438621429316 1103 94-95 0.11912847924999781 1103 96-97 0.1244332509143895 1103 98-99 0.08017031778062744 1103 100-101 0.08348088578355473 1103 102-103 -0.021325659362073424 1103 104-105 0.04503723501596468 1103 106-107 0.05270407729099702 1103 108-109 -0.011279470285110449 1103 110-111 0.013054288069863418 1103 112-113 0.07210829321088141 1103 114-115 0.05840122242797818 1103 116-117 0.06974624048368128 1103 118-119 -0.03189133260629262 1103 120-121 -0.04326641293990008 1103 122-123 -0.0128952105036646 1103 124-125 -0.04373625319206198 1104 1 0.16475224550348955 1104 2 0.1367229478183809 1104 3 0.1502465984945225 1104 4 0.19892531018331283 1104 5 0.12217533115608603 1104 6 0.16870550050433764 1104 7 0.1632952297329595 1104 8 0.1788558053844369 1104 9 0.3487561391733536 1104 10-11 0.267741368969304 1104 12-13 0.24041462933627855 1104 14-15 0.24328099708063888 1104 16-17 0.25733245404990157 1104 18-19 0.44398929001190623 1104 20-21 0.4505502214221906 1104 22-23 0.4274660046063943 1104 24-25 0.6049808834094108 1104 26-27 0.5943630973885377 1104 28-29 0.4290482332041634 1104 30-31 0.4323242195204955 1104 32-33 0.5711072803289383 1104 34-35 0.5631624902574828 1104 36-37 0.49074366603975506 1104 38-39 0.5772901715587082 1104 40-41 0.5407948484086234 1104 42-43 0.42878330362221106 1104 44-45 0.32268924093071405 1104 46-47 0.28587053754629466 1104 48-49 0.250294801693677 1104 50-51 0.2578046248381085 1104 52-53 0.2379853607142124 1104 54-55 0.25805004089747996 1104 56-57 0.22011057691415914 1104 58-59 0.19611362217094808 1104 60-61 0.2182090757438857 1104 62-63 0.24726720070852082 1104 64-65 0.17239199868409827 1104 66-67 0.23421358077887788 1104 68-69 0.27275894253025257 1104 70-71 0.22559415779306136 1104 72-73 0.21552990956937634 1104 74-75 0.19542583769705146 1104 76-77 0.17476151476295598 1104 78-79 0.23573576482994696 1104 80-81 0.14182547728851347 1104 82-83 0.14330320316211242 1104 84-85 0.18394703305779814 1104 86-87 0.1731464737637296 1104 88-89 0.17600162352850646 1104 90-91 0.13289010019855496 1104 92-93 0.10632419324649334 1104 94-95 0.13669411553246036 1104 96-97 0.0972112129061955 1104 98-99 0.021452357900592744 1104 100-101 0.12045810643223476 1104 102-103 0.06736348594451158 1104 104-105 0.10346607663491625 1104 106-107 0.1487538132268753 1104 108-109 0.12880261095883583 1104 110-111 0.10095198197777222 1104 112-113 0.1703919227171511 1104 114-115 0.16724709270960503 1104 116-117 0.12506767441393052 1104 118-119 0.031155991463649002 1104 120-121 0.01415919803834953 1104 122-123 0.08350796938040972 1104 124-125 0.049142351408267615 1105 1 0.12883875370536657 1105 2 0.14041954415952773 1105 3 0.19771467868273263 1105 4 0.13287873486668644 1105 5 0.08158181510724916 1105 6 0.09796875791865745 1105 7 0.05595464728526167 1105 8 0.08127351836868968 1105 9 0.08264119635417444 1105 10-11 0.08442875616159284 1105 12-13 0.10546372777320556 1105 14-15 -0.027853227260514757 1105 16-17 0.01425962397517111 1105 18-19 0.16272457040140864 1105 20-21 0.22142593225648 1105 22-23 0.10858830378308681 1105 24-25 0.37647945564612684 1105 26-27 0.3968837353167629 1105 28-29 0.23717695931757987 1105 30-31 0.2830604958832197 1105 32-33 0.46700031881955795 1105 34-35 0.4159744819387896 1105 36-37 0.3390263774073503 1105 38-39 0.29477634813726894 1105 40-41 0.25833583287574413 1105 42-43 0.19860091524746792 1105 44-45 0.1043020039796616 1105 46-47 0.09435340180655061 1105 48-49 0.08483334507439366 1105 50-51 -0.025147839639529934 1105 52-53 -0.024730225136742945 1105 54-55 0.036235064321623156 1105 56-57 0.010962650956763298 1105 58-59 -0.003550159014615417 1105 60-61 -0.11879775780330704 1105 62-63 -0.04864873075243992 1105 64-65 -0.03287308643094633 1105 66-67 0.06479209850665058 1105 68-69 0.04148640211877819 1105 70-71 0.0225986175180779 1105 72-73 0.049944846035820945 1105 74-75 -0.03018818860254413 1105 76-77 0.019408888281738257 1105 78-79 -0.01839259933786508 1105 80-81 -0.05223806765368266 1105 82-83 0.04583088781888023 1105 84-85 -0.00963523011295564 1105 86-87 -0.08245941231878362 1105 88-89 -0.06649774787127427 1105 90-91 -0.04288322487326113 1105 92-93 -0.08984798733695953 1105 94-95 -0.08437552714323715 1105 96-97 -0.058838367946915326 1105 98-99 -0.07978023681340218 1105 100-101 -0.015534545395848198 1105 102-103 -0.18452302649251706 1105 104-105 -0.13451128806299906 1105 106-107 -0.030467737697573938 1105 108-109 -0.10580562563471929 1105 110-111 -0.15501642803648608 1105 112-113 -0.10665603721044903 1105 114-115 -0.03196772645372192 1105 116-117 -0.0880872108996762 1105 118-119 -0.20659190968783747 1105 120-121 -0.12453752464565682 1105 122-123 -0.09425810289944891 1105 124-125 -0.07626076684028504 1106 1 0.15715107799114136 1106 2 0.2183363661920552 1106 3 0.18529554223241007 1106 4 0.11866679426199056 1106 5 0.03246011490080036 1106 6 0.08048937926007227 1106 7 0.003305111569055441 1106 8 -0.002030533196553108 1106 9 0.0018711286092738533 1106 10-11 0.11185632949788982 1106 12-13 0.09650168034551143 1106 14-15 0.10639007943952805 1106 16-17 0.14361035974073388 1106 18-19 0.18330327498662768 1106 20-21 0.19487943609067315 1106 22-23 0.13983396216353583 1106 24-25 0.3821495661622123 1106 26-27 0.39708801842246544 1106 28-29 0.34158101421231635 1106 30-31 0.3489413881051604 1106 32-33 0.4619324351509704 1106 34-35 0.47096261941135253 1106 36-37 0.30805618947319857 1106 38-39 0.3493561713147173 1106 40-41 0.2160555908832933 1106 42-43 0.2737139931155781 1106 44-45 0.1836827534700447 1106 46-47 0.1750542975410454 1106 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-0.7411974364630112 2216 94-95 -0.5956504418500437 2216 96-97 -0.7335936411588513 2216 98-99 -0.68202216861593 2216 100-101 -0.6541575614020054 2216 102-103 -0.7117253523654945 2216 104-105 -0.6927043294716988 2216 106-107 -0.6749036702364855 2216 108-109 -0.6421396926278433 2216 110-111 -0.6781729284638338 2216 112-113 -0.7053040771887638 2216 114-115 -0.7136713376845236 2216 116-117 -0.697030226482017 2216 118-119 -0.5872645195800814 2216 120-121 -0.395736574986298 2216 122-123 -0.3622380746714633 2216 124-125 -0.22587587689146105 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 44138.0 3 8263.0 4 5691.0 5 4683.0 6 5156.0 7 5752.0 8 6296.0 9 6769.0 10 7853.0 11 8863.0 12 9605.0 13 9341.0 14 9190.0 15 9201.0 16 8498.0 17 8186.0 18 7627.0 19 7307.0 20 7527.0 21 7574.0 22 8950.0 23 11656.0 24 17268.0 25 25611.0 26 30103.0 27 30958.0 28 36387.0 29 44263.0 30 53134.0 31 65358.0 32 85055.0 33 115529.0 34 168712.0 35 272352.0 36 553962.0 37 978757.0 38 375627.0 39 950.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.86629667003027 17.82166440973947 12.42990672529449 24.882132194935767 2 16.193286706523594 19.484717772316273 39.26463774915752 25.057357772002607 3 19.22363301510176 23.996420507395364 28.49444044944261 28.28550602806027 4 12.896179291007776 15.850634824539078 36.188837634488344 35.064348249964794 5 14.40693830997944 37.59993700999378 33.62555512393317 14.367569556093612 6 33.724764158491766 35.426291323292446 17.047211410529147 13.801733107686639 7 29.530912562044087 31.644240607131557 20.584415393563386 18.24043143726097 8 26.486275011821753 35.49043287204554 19.266319895289328 18.75697222084338 9 26.61809253968814 16.14098579939937 18.969969864055148 38.27095179685735 10-11 25.40191203877049 26.13004260750136 27.4625618811376 21.005483472590555 12-13 25.817675861012656 23.485800259498255 27.440960555024507 23.255563324464585 14-15 22.894724208013937 25.21970899682582 24.598981479092497 27.28658531606775 16-17 23.625460373393338 28.138609641735407 25.172576237670647 23.06335374720061 18-19 23.459568327561186 27.144037287051216 26.389246546631924 23.007147838755678 20-21 24.184561842071528 26.4887026099214 26.236696909419205 23.090038638587867 22-23 23.915300036876058 26.366986357164716 26.122980513174944 23.594733092784278 24-25 23.609555767833378 26.6272646369641 26.24691176590655 23.516267829295973 26-27 23.65579895764453 26.82063944178636 26.096709801188812 23.426851799380298 28-29 23.50010483638665 26.737393669449894 26.2129994506704 23.549502043493057 30-31 23.690315227047083 26.479137273344826 26.18837200188117 23.642175497726917 32-33 23.464353805567875 26.618106859878267 26.356094218739894 23.561445115813964 34-35 23.798391148047955 26.642135615741246 25.99372477186482 23.56574846434598 36-37 23.99607203533143 26.54946137466025 25.864860181801465 23.589606408206855 38-39 23.635533044004884 26.46102591608517 26.197873350968298 23.70556768894165 40-41 23.830285525144152 26.49462949237162 26.08204988023173 23.5930351022525 42-43 23.768902035985715 26.4911315879539 25.983147535158828 23.756818840901552 44-45 23.656023361409524 26.64299915353426 25.989633407181373 23.71134407787485 46-47 23.77990574377735 26.642470687398983 25.83783762600508 23.73978594281859 48-49 23.82306831016768 26.626966560370448 25.894562792360194 23.65540233710168 50-51 23.597140322462394 26.60860943664849 25.972361719196364 23.821888521692756 52-53 23.800146468468867 26.523137364400323 25.915693271839434 23.761022895291372 54-55 23.681923550978098 26.30960149080883 25.9686109755771 24.03986398263597 56-57 23.60246905597243 26.592448680806818 26.053106061570947 23.751976201649803 58-59 23.74019458233579 26.411876449573736 25.982909583830775 23.865019384259696 60-61 23.762760747117145 26.42125857589164 26.106671761983385 23.70930891500783 62-63 23.693533139075633 26.448175474048174 26.02741806498624 23.83087332188995 64-65 23.529209095093726 26.44372586700787 25.94826013853054 24.07880489936786 66-67 23.55295066272525 26.612814337484576 25.856727374502185 23.977507625287984 68-69 23.357804172861947 26.346806584019404 26.258639708734137 24.036749534384512 70-71 23.434312557977613 26.178146744411574 26.23247281940899 24.155067878201823 72-73 23.53628722270955 26.365559322830972 26.001831457569818 24.096321996889657 74-75 23.3485161820211 26.519295879403714 25.955617313835933 24.176570624739252 76-77 23.533305618519854 26.442169057531967 25.951956490573096 24.07256883337508 78-79 23.53500010450522 26.39622239755876 26.080583975169557 23.988193522766462 80-81 23.51564650821327 26.569410693530525 26.087936965249174 23.82700583300703 82-83 23.48944971026382 26.538864228946444 26.05052225665035 23.921163804139386 84-85 23.64486938237408 26.310213942508287 25.924017087443076 24.120899587674558 86-87 23.523337163706742 26.121535456645883 26.21175634514119 24.143371034506185 88-89 23.457162862179572 26.230125476554218 26.128413767608095 24.184297893658112 90-91 23.56466609636433 26.38740140200322 26.144987530691576 23.90294497094087 92-93 23.504199117460335 26.030886928850794 26.228792138133915 24.236121815554956 94-95 23.450583119322623 26.062128855785083 26.281239468190996 24.2060485567013 96-97 23.44181612707756 26.089199939192024 26.228761093086977 24.240222840643437 98-99 23.42051739448353 25.943857931045972 26.070566384172256 24.565058290298243 100-101 23.188340709774742 26.060588343943316 26.27282604559649 24.47824490068546 102-103 23.347841592927868 25.812101109952074 26.420703385507327 24.419353911612728 104-105 23.324483121615238 25.942883962717406 26.24480802639843 24.487824889268925 106-107 23.224091824674353 26.007648136085336 26.378457946031837 24.389802093208473 108-109 23.304097558681644 26.00252570444751 26.095255699000298 24.598121037870545 110-111 23.05874042586266 26.222918623731072 26.246382985741278 24.471957964664988 112-113 23.152522958066733 26.200653282769597 26.377112467947878 24.269711291215796 114-115 23.122403558649808 26.244127636540515 26.184509733398535 24.448959071411142 116-117 23.09430040199956 26.206541874232474 26.340127861573748 24.359029862194216 118-119 22.958931692431925 26.253362876724946 26.159289175772805 24.628416255070327 120-121 23.0942657422225 26.2971571069547 26.239677696795454 24.368899454027343 122-123 23.066945613527942 26.259613296031542 26.213671989194893 24.459769101245627 124-125 23.097118191872443 26.385196139068995 25.94411910390339 24.57356656515518 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 855.0 1 705.0 2 504.5 3 408.0 4 324.0 5 271.0 6 236.0 7 221.5 8 221.5 9 230.5 10 234.0 11 226.5 12 235.5 13 254.5 14 285.0 15 326.0 16 389.0 17 470.0 18 612.5 19 804.0 20 1047.5 21 1485.0 22 2059.5 23 2830.5 24 4200.0 25 6185.0 26 8759.5 27 12221.0 28 16032.5 29 20324.0 30 25777.5 31 31711.0 32 37383.5 33 42937.5 34 49773.0 35 55947.5 36 61692.5 37 67757.5 38 72438.0 39 75510.0 40 79405.5 41 85927.0 42 91366.5 43 97405.5 44 102282.0 45 106295.0 46 110250.0 47 113253.0 48 117688.5 49 119286.0 50 117649.5 51 115764.0 52 117007.0 53 118668.5 54 117891.5 55 114469.5 56 108143.5 57 101071.5 58 95101.0 59 86073.0 60 72036.0 61 58322.0 62 47265.5 63 37884.0 64 30177.5 65 23976.0 66 18467.5 67 15541.5 68 12296.5 69 7964.0 70 5298.0 71 3884.0 72 2956.0 73 2240.0 74 1579.0 75 1258.5 76 988.0 77 558.5 78 307.0 79 187.5 80 119.5 81 84.5 82 48.0 83 33.0 84 32.5 85 34.5 86 26.5 87 15.5 88 15.0 89 16.5 90 15.5 91 10.5 92 7.5 93 10.0 94 9.5 95 6.0 96 6.0 97 4.5 98 3.0 99 3.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06348476496267984 2 0.0502261154900214 3 0.04516431581449909 4 0.04522962935869937 5 0.04405398556309419 6 0.004506634549819866 7 4.898515815021593E-4 8 0.0 9 9.143896188040306E-4 10-11 0.004327022303269074 12-13 0.0029064527169128116 14-15 0.008523417518137571 16-17 0.003249348823964323 18-19 0.0057965770477755515 20-21 0.01033586836969556 22-23 0.017797940794578453 24-25 0.004147410056718282 26-27 0.01725910405492608 28-29 0.00777231175983426 30-31 0.007576371127233398 32-33 0.00280848240061238 34-35 0.019267495539084932 36-37 0.016834566017624206 38-39 0.0264519854011166 40-41 0.006449712489778429 42-43 0.0015675250608069096 44-45 2.612541768011516E-4 46-47 0.0023839443633105087 48-49 0.003641230089166051 50-51 0.0019104211678584212 52-53 0.001943077939958565 54-55 0.00620478669902735 56-57 0.0029717662611130997 58-59 0.0085070891320875 60-61 0.016883551175774423 62-63 0.013438261719209236 64-65 0.00272684047036202 66-67 0.004457649391669649 68-69 0.01125025798849959 70-71 0.002824810786662452 72-73 0.003592244931015835 74-75 0.0011266586374549664 76-77 3.5922449310158344E-4 78-79 0.003396304298414971 80-81 0.003102393349513675 82-83 0.0018287792376080613 84-85 0.012131990835203478 86-87 0.0072661317922820285 88-89 0.0021553469586095006 90-91 0.0010940018653548223 92-93 0.002612541768011516 94-95 0.0 96-97 0.0017471373073577015 98-99 0.025913148661464224 100-101 0.01601814671512061 102-103 0.0071191763178313815 104-105 0.014858831305565498 106-107 0.03311396690954597 108-109 0.0011756437956051823 110-111 0.0020083914841588528 112-113 0.017553015003827374 114-115 0.03236286115124266 116-117 0.05148340121587694 118-119 0.3533789308956577 120-121 0.11970339813307765 122-123 0.020802363827791697 124-125 0.3142887746917854 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3062152.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.2166240979807 #Duplication Level Percentage of deduplicated Percentage of total 1 84.3734862118136 62.61915310018327 2 9.692075634413138 14.386262681768752 3 2.9295135721063024 6.522558227128384 4 1.1682304170588473 3.4680847089073477 5 0.5900582170927549 2.189606444694884 6 0.3459821748337823 1.5406577408544395 7 0.22535251058177205 1.1707431797168515 8 0.1560992965520444 0.9268130251329837 9 0.09512703852078999 0.6354006893506441 >10 0.4005529860737822 4.974790193962257 >50 0.01862478001377765 0.9644735539574126 >100 0.004804771134770989 0.5173227894468521 >500 0.0 0.0 >1k 9.23898046838707E-5 0.08413366489577559 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 9.797031630043185E-5 0.0 0.0 0.0 6 0.0 9.797031630043185E-5 0.0 0.0 0.0 7 3.265677210014395E-5 9.797031630043185E-5 0.0 0.0 0.0 8 6.53135442002879E-5 9.797031630043185E-5 0.0 0.0 0.0 9 6.53135442002879E-5 9.797031630043185E-5 0.0 0.0 3.265677210014395E-5 10-11 9.797031630043185E-5 9.797031630043185E-5 0.0 0.0 3.265677210014395E-5 12-13 1.1429870235050383E-4 1.306270884005758E-4 0.0 0.0 3.265677210014395E-5 14-15 1.306270884005758E-4 1.6328386050071977E-4 0.0 0.0 4.8985158150215925E-5 16-17 1.469554744506478E-4 1.6328386050071977E-4 0.0 1.6328386050071976E-5 6.53135442002879E-5 18-19 2.4492579075107964E-4 1.959406326008637E-4 0.0 6.53135442002879E-5 6.53135442002879E-5 20-21 3.2656772100143953E-4 1.959406326008637E-4 0.0 1.306270884005758E-4 6.53135442002879E-5 22-23 3.5922449310158344E-4 1.959406326008637E-4 0.0 2.4492579075107964E-4 6.53135442002879E-5 24-25 6.531354420028791E-4 2.2859740470100766E-4 0.0 5.551651257024472E-4 8.164193025035987E-5 26-27 6.531354420028791E-4 2.612541768011516E-4 0.0 0.0010940018653548223 1.1429870235050383E-4 28-29 8.817328467038867E-4 2.612541768011516E-4 0.0 0.0024002727493605807 1.306270884005758E-4 30-31 9.470463909041746E-4 2.612541768011516E-4 0.0 0.00669463828052951 1.306270884005758E-4 32-33 9.470463909041746E-4 2.612541768011516E-4 0.0 0.01729176082702622 1.306270884005758E-4 34-35 0.0010123599351044626 2.612541768011516E-4 0.0 0.03448555133775201 1.306270884005758E-4 36-37 0.0011103302514048944 2.939109489012956E-4 0.0 0.06417055717678286 1.306270884005758E-4 38-39 0.0012083005677053262 3.428961070515115E-4 0.0 0.10536707518111446 1.306270884005758E-4 40-41 0.0012083005677053262 3.918812652017274E-4 0.0 0.14888222400455628 1.306270884005758E-4 42-43 0.0012409573398054702 3.918812652017274E-4 0.0 0.1916299386836447 1.306270884005758E-4 44-45 0.0013062708840057581 3.918812652017274E-4 0.0 0.24742403381674064 1.306270884005758E-4 46-47 0.0014858831305565499 3.918812652017274E-4 0.0 0.3154317617152904 1.306270884005758E-4 48-49 0.0015022115166066217 3.918812652017274E-4 0.0 0.3868357939122552 1.306270884005758E-4 50-51 0.0015022115166066217 4.2453803730187136E-4 0.0 0.46524470372470084 1.306270884005758E-4 52-53 0.0015022115166066217 4.2453803730187136E-4 0.0 0.5444373760675498 1.6328386050071974E-4 54-55 0.0015348682887067657 4.735231954520873E-4 0.0 0.6318758833656853 1.959406326008637E-4 56-57 0.0016165102189571257 5.225083536023032E-4 0.0 0.7355448063975922 1.959406326008637E-4 58-59 0.0016328386050071975 5.551651257024472E-4 0.0 0.8536316943117128 1.959406326008637E-4 60-61 0.0016328386050071975 5.551651257024472E-4 0.0 0.9875407883083531 2.2859740470100766E-4 62-63 0.0016328386050071975 5.551651257024472E-4 0.0 1.1188373405369818 2.2859740470100766E-4 64-65 0.0016328386050071975 5.714935117525192E-4 0.0 1.2603391340469057 2.2859740470100766E-4 66-67 0.0017308089213076295 6.204786699027351E-4 0.0 1.4277377478322435 2.612541768011516E-4 68-69 0.0017634656934077733 6.204786699027351E-4 0.0 1.617751176296931 3.1023933495136755E-4 70-71 0.0018287792376080613 6.69463828052951E-4 0.0 1.8332532153857808 3.5922449310158344E-4 72-73 0.001894092781808349 7.347773722532389E-4 0.0 2.052706723898748 5.551651257024472E-4 74-75 0.001926749553908493 7.511057583033108E-4 0.0 2.277271017245388 5.551651257024472E-4 76-77 0.001926749553908493 7.837625304034548E-4 0.0 2.5259686651740347 5.551651257024472E-4 78-79 0.001926749553908493 8.327476885536707E-4 0.0 2.8104907920965383 5.551651257024472E-4 80-81 0.0021880037307096447 8.817328467038867E-4 0.0 3.122918130778616 6.041502838526631E-4 82-83 0.002253317274909933 8.817328467038867E-4 0.0 3.450269614310459 6.85792214103023E-4 84-85 0.0023186308191102205 8.817328467038867E-4 0.0 3.7803316099266135 7.184489862031669E-4 86-87 0.0024002727493605803 9.143896188040306E-4 0.0 4.123995151122479 7.184489862031669E-4 88-89 0.0024166011354106523 9.143896188040306E-4 0.0 4.507287685261868 7.184489862031669E-4 90-91 0.002530899837761156 9.143896188040306E-4 0.0 4.9343239656294005 7.184489862031669E-4 92-93 0.002547228223811228 9.143896188040306E-4 0.0 5.387093782411846 7.184489862031669E-4 94-95 0.002547228223811228 9.143896188040306E-4 0.0 5.847635910954127 7.511057583033108E-4 96-97 0.002547228223811228 9.143896188040306E-4 0.0 6.315150260339787 7.511057583033108E-4 98-99 0.002710512084311948 9.143896188040306E-4 0.0 6.8083165042101115 7.511057583033108E-4 100-101 0.0029391094890129556 9.143896188040306E-4 0.0 7.361342611339999 7.511057583033108E-4 102-103 0.0031677068937139632 9.797031630043185E-4 0.0 7.945947817090725 7.511057583033108E-4 104-105 0.003428961070515115 9.797031630043185E-4 0.0 8.53452082065162 7.511057583033108E-4 106-107 0.003526931386815547 9.797031630043185E-4 0.0 9.113427419670872 7.674341443533828E-4 108-109 0.003592244931015835 0.0010123599351044624 0.0 9.720729082031198 7.837625304034548E-4 110-111 0.0036575584752161225 0.0010123599351044624 0.0 10.373293030522326 8.654044606538147E-4 112-113 0.0038208423357168424 0.0010123599351044624 0.0 11.06898024657169 9.470463909041746E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2590 0.0 48.237156 1 GTATCAA 4155 0.0 45.102615 1 TCAACGC 4560 0.0 39.78888 4 ATCAACG 4565 0.0 39.745296 3 CAACGCA 4725 0.0 38.399426 5 AACGCAG 4835 0.0 37.266956 6 GTACATG 11480 0.0 36.586845 1 TATCAAC 5335 0.0 34.67789 2 TACATGG 12165 0.0 33.692543 2 ACATGGG 11690 0.0 33.331394 3 ACGCAGA 6050 0.0 29.585695 7 CGCAGAG 6425 0.0 27.488686 8 CATGGGG 7540 0.0 26.193493 4 GAGTACA 8455 0.0 23.360743 1 AGTACAT 8275 0.0 22.788616 2 CATGGGA 6070 0.0 22.736626 4 GCAGAGT 8155 0.0 21.222147 9 AGAGTAC 6500 0.0 20.358086 10-11 ATGGGGA 4435 0.0 19.851566 5 TAGTGCG 275 1.3251265E-6 19.463911 9 >>END_MODULE