FastQCFastQC Report
Thu 26 May 2016
SRR1364199_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364199_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3062152
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53290.17402793852166712No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47650.15560951905718592No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43350.066.333441
GTATCAA76950.052.6881561
ATCAACG96050.041.8824653
TCAACGC96850.041.4750674
CAACGCA98750.040.66715
AACGCAG101450.039.936656
TATCAAC101050.039.8101042
ACGCAGA123850.032.6148077
CGCAGAG124850.032.0676848
GTACATG110950.031.659151
TACATGG114900.030.142962
ACATGGG113300.029.5181663
GCAGAGT141550.027.8640829
GTGGTAT24650.027.050521
GAGTACT82500.024.80576712-13
TGGTATC25100.024.6571332
AGAGTAC127750.024.63405410-11
CATGGGG72950.024.3093834
CAGAGTA137950.023.80446810-11
AGTACTT87250.022.9098412-13
Produced by FastQC (version 0.11.5)