FastQCFastQC Report
Thu 26 May 2016
SRR1364198_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364198_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1952747
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29080.1489184210755413No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18450.052.892011
GTATCAA32500.041.7440031
TCAACGC32300.039.0509344
GTACATG79950.038.701471
ATCAACG33850.037.614323
CAACGCA33550.037.595985
TACATGG81900.036.833622
ACATGGG80250.035.068263
AACGCAG36500.035.0311626
TATCAAC41300.031.695162
CATGGGG37850.028.766254
ACGCAGA43800.028.3772327
CGCAGAG45100.027.1636758
CATGGGA51350.026.1858124
TGTACCG2001.8276296E-623.7989655
CGTAGAC2153.4256627E-622.138573
GAGTACA56900.021.6471481
ATGGGGG22900.021.304755
AGTACAT54950.021.2230932
GCAGAGT56100.021.0975769
Produced by FastQC (version 0.11.5)