##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364198_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1952747 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.036052801514995 33.0 31.0 34.0 28.0 34.0 2 31.062857605209484 33.0 31.0 34.0 27.0 34.0 3 31.14757006411993 34.0 31.0 34.0 27.0 34.0 4 34.49545998534372 37.0 35.0 37.0 31.0 37.0 5 34.6114647724462 37.0 35.0 37.0 32.0 37.0 6 34.67259250686341 37.0 35.0 37.0 32.0 37.0 7 34.58080693505098 37.0 35.0 37.0 31.0 37.0 8 34.54193093114469 37.0 35.0 37.0 31.0 37.0 9 36.02585229935061 39.0 37.0 39.0 31.0 39.0 10-11 35.999493790030144 39.0 37.0 39.0 31.0 39.0 12-13 35.95256784417029 39.0 37.0 39.0 31.0 39.0 14-15 37.27841701971633 40.0 38.0 41.0 31.0 41.0 16-17 37.32028726711653 40.0 38.0 41.0 31.0 41.0 18-19 37.23435626837475 40.0 38.0 41.0 30.5 41.0 20-21 37.2193924763423 40.0 38.0 41.0 30.0 41.0 22-23 37.271907727934035 40.0 38.0 41.0 30.5 41.0 24-25 37.14059348190011 40.0 37.0 41.0 30.0 41.0 26-27 37.168652160264486 40.0 37.0 41.0 30.0 41.0 28-29 37.26134517169915 40.0 37.0 41.0 30.0 41.0 30-31 37.16240045433433 40.0 37.0 41.0 30.0 41.0 32-33 36.98030518034338 40.0 37.0 41.0 30.0 41.0 34-35 36.93532956394249 40.0 37.0 41.0 30.0 41.0 36-37 36.967385944006054 40.0 37.0 41.0 30.0 41.0 38-39 36.81350784305391 40.0 37.0 41.0 29.5 41.0 40-41 36.82693047281599 40.0 37.0 41.0 30.0 41.0 42-43 36.76626157920099 40.0 37.0 41.0 30.0 41.0 44-45 36.76957306809331 40.0 37.0 41.0 30.0 41.0 46-47 36.71549181742438 40.0 36.5 41.0 30.0 41.0 48-49 36.64787553123881 40.0 36.0 41.0 30.0 41.0 50-51 36.5525272859208 40.0 36.0 41.0 30.0 41.0 52-53 36.42612407034808 40.0 36.0 41.0 29.5 41.0 54-55 36.26429742306607 40.0 35.0 41.0 29.0 41.0 56-57 36.095659345527096 39.0 35.0 41.0 29.0 41.0 58-59 35.886548795107615 39.0 35.0 41.0 29.0 41.0 60-61 35.65962846185399 39.0 35.0 41.0 28.0 41.0 62-63 35.383590142501816 38.5 35.0 40.5 28.0 41.0 64-65 35.09499156828816 38.0 34.0 40.0 28.0 41.0 66-67 34.803953097866746 37.0 34.0 40.0 28.0 41.0 68-69 34.578317365229594 37.0 34.0 40.0 28.0 41.0 70-71 34.414043396302745 36.5 34.0 39.0 28.0 41.0 72-73 34.0948282086722 36.0 34.0 39.0 28.0 41.0 74-75 33.74870758987211 36.0 34.0 39.0 27.5 40.5 76-77 33.40287400262297 35.0 34.0 37.5 27.0 39.5 78-79 33.04741192791488 35.0 34.0 37.0 26.5 39.0 80-81 32.72241821393145 35.0 33.0 36.5 26.0 39.0 82-83 32.4256508907708 35.0 33.0 36.0 26.0 38.0 84-85 32.1517288209891 35.0 33.0 36.0 26.0 37.0 86-87 31.919283450441863 35.0 33.0 35.5 26.0 37.0 88-89 31.72774353257232 35.0 33.0 35.0 25.0 36.0 90-91 31.556624078797714 35.0 33.0 35.0 25.0 36.0 92-93 31.40859568597468 35.0 33.0 35.0 25.0 36.0 94-95 31.265993239267555 35.0 33.0 35.0 24.0 36.0 96-97 31.15885416800026 35.0 33.0 35.0 24.0 35.5 98-99 31.07260054681943 35.0 32.5 35.0 23.5 35.0 100-101 30.965587707982653 35.0 32.0 35.0 23.0 35.0 102-103 30.000588913976053 34.0 30.5 35.0 19.5 35.0 104-105 30.661445645544454 34.5 32.0 35.0 20.0 35.0 106-107 30.750632314375594 35.0 32.0 35.0 20.0 35.0 108-109 30.706023104887628 35.0 32.0 35.0 19.0 35.0 110-111 30.624968825966704 35.0 32.0 35.0 19.0 35.0 112-113 30.753542317565973 35.0 32.5 35.0 19.0 35.0 114-115 30.726033633645322 35.0 33.0 35.0 18.5 35.0 116-117 30.62324382011597 35.0 33.0 35.0 16.5 35.0 118-119 30.375697927074015 35.0 32.0 35.0 2.0 35.0 120-121 30.30263623500638 35.0 32.0 35.0 2.0 35.0 122-123 30.15705106703531 35.0 32.0 35.0 2.0 35.0 124-125 29.296625471707294 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.48304544403250915 1101 2 0.6499879844066889 1101 3 0.6308495197127186 1101 4 0.6305641912169051 1101 5 0.4143726136875472 1101 6 0.5434490853027114 1101 7 0.6132806121405991 1101 8 0.5847176706953263 1101 9 0.5980151314286175 1101 10-11 0.6085864989482346 1101 12-13 0.5959280445332382 1101 14-15 0.6680645348532437 1101 16-17 0.6711456927953776 1101 18-19 0.7358466379654516 1101 20-21 0.7911058938741888 1101 22-23 0.7793355365437691 1101 24-25 0.9092950191866294 1101 26-27 0.9403221837502471 1101 28-29 0.8356916923829587 1101 30-31 0.8801217115024116 1101 32-33 1.031891256150061 1101 34-35 0.9621311714188181 1101 36-37 0.9612795401210761 1101 38-39 0.9932431541585416 1101 40-41 0.8705830945330533 1101 42-43 0.7702980271028963 1101 44-45 0.793463761592264 1101 46-47 0.7383849567647331 1101 48-49 0.6582750811625786 1101 50-51 0.6202085466055607 1101 52-53 0.559691241576715 1101 54-55 0.6335758135608245 1101 56-57 0.5637622766773092 1101 58-59 0.5124182407564106 1101 60-61 0.4780474523507081 1101 62-63 0.5014399734526478 1101 64-65 0.4594807272918118 1101 66-67 0.4201810844695899 1101 68-69 0.3932271081741163 1101 70-71 0.4711195523224987 1101 72-73 0.4349649419710957 1101 74-75 0.32617859477371525 1101 76-77 0.38099827242405127 1101 78-79 0.29935159039511205 1101 80-81 0.30695609881924213 1101 82-83 0.31850373793606934 1101 84-85 0.16861979581059217 1101 86-87 0.23115962079415908 1101 88-89 0.18064407658044246 1101 90-91 0.15429702533288392 1101 92-93 0.13912705048589658 1101 94-95 0.1697377183858393 1101 96-97 0.06763005737110817 1101 98-99 0.017175024296690822 1101 100-101 -0.006036906402201225 1101 102-103 -0.045400903617849764 1101 104-105 0.12472226472201342 1101 106-107 0.05185559430974962 1101 108-109 0.021046657020306725 1101 110-111 -0.07259901421565118 1101 112-113 0.04760542320013883 1101 114-115 0.05976263731048448 1101 116-117 0.06980841283874994 1101 118-119 -0.2373732194592506 1101 120-121 -0.2190188262959758 1101 122-123 -0.2365295224689241 1101 124-125 -0.41095970649098845 1102 1 0.1186249893848128 1102 2 0.12403187522053827 1102 3 0.003724031423974594 1102 4 0.09884573238081629 1102 5 -0.0013464071999109706 1102 6 0.1268201709880259 1102 7 0.05350681929409973 1102 8 0.09493065217674257 1102 9 0.04735853158538106 1102 10-11 0.18961960705526337 1102 12-13 0.13201020776417494 1102 14-15 0.11557643875198664 1102 16-17 0.10368616499262373 1102 18-19 0.19367293868833002 1102 20-21 0.2158428755313082 1102 22-23 0.28696351834008027 1102 24-25 0.41543630365924145 1102 26-27 0.3810487198255359 1102 28-29 0.1985413007728738 1102 30-31 0.35762000867728716 1102 32-33 0.44241864504039086 1102 34-35 0.4219685125877035 1102 36-37 0.2609290651029923 1102 38-39 0.38175779051606185 1102 40-41 0.3149647507005042 1102 42-43 0.27253372932575104 1102 44-45 0.07986823111791352 1102 46-47 0.12185343557957395 1102 48-49 0.15501040683218292 1102 50-51 0.04134147969340063 1102 52-53 0.08779817634858489 1102 54-55 0.10325864617271918 1102 56-57 0.1937067542579598 1102 58-59 0.09543334734242137 1102 60-61 0.03116521005152606 1102 62-63 0.07087693505162918 1102 64-65 0.11495322620290693 1102 66-67 0.07332903523134604 1102 68-69 0.15029178165772805 1102 70-71 0.11380577947063131 1102 72-73 0.07501357635032235 1102 74-75 0.08949913288233091 1102 76-77 0.06481546653870396 1102 78-79 0.10446047594038532 1102 80-81 0.10314288193251286 1102 82-83 -0.004448293996070163 1102 84-85 0.05406623766023699 1102 86-87 0.06140376648930612 1102 88-89 0.14082046121410485 1102 90-91 0.024983929377164316 1102 92-93 0.06193733909557153 1102 94-95 0.008332851931257323 1102 96-97 0.08041097059344438 1102 98-99 0.00703038074681217 1102 100-101 -0.05119848823280648 1102 102-103 -0.034297213607704435 1102 104-105 0.019448640438010756 1102 106-107 0.07046335813386051 1102 108-109 0.009182120397788651 1102 110-111 0.03875589211705588 1102 112-113 0.007979346133474507 1102 114-115 0.07535850438057423 1102 116-117 0.06458257879843998 1102 118-119 0.06966004371469126 1102 120-121 0.1581728168151635 1102 122-123 0.07408020271335758 1102 124-125 0.06068620432066396 1103 1 0.43782104101565267 1103 2 0.08705039074676435 1103 3 0.42921451372169983 1103 4 0.4605499028821711 1103 5 0.37845332776102225 1103 6 0.4292392184137839 1103 7 0.5525841523651565 1103 8 0.42197171449787163 1103 9 0.5446881055583646 1103 10-11 0.5854067062148971 1103 12-13 0.5971833691369568 1103 14-15 0.7634822111967168 1103 16-17 0.6178350255666132 1103 18-19 0.760275453202901 1103 20-21 0.80884912005574 1103 22-23 0.7467994054000258 1103 24-25 0.9861862658652853 1103 26-27 0.9464758487831872 1103 28-29 0.8204906259068068 1103 30-31 0.8185184198219346 1103 32-33 1.0863573529996557 1103 34-35 1.061925454797688 1103 36-37 0.8358904189931096 1103 38-39 1.0624998764874931 1103 40-41 1.0383232635258608 1103 42-43 0.9164207877121484 1103 44-45 0.7759900507336681 1103 46-47 0.784915235269267 1103 48-49 0.7984867405295333 1103 50-51 0.7218835374071801 1103 52-53 0.7845866267234953 1103 54-55 0.6743388664158658 1103 56-57 0.6260377476184544 1103 58-59 0.558852244633151 1103 60-61 0.6585300644556682 1103 62-63 0.5976602959270636 1103 64-65 0.5397363565417095 1103 66-67 0.48761663410385125 1103 68-69 0.5428823967351875 1103 70-71 0.5172186063103013 1103 72-73 0.5351403012444038 1103 74-75 0.4946506118424878 1103 76-77 0.5713875169122602 1103 78-79 0.49392381996531043 1103 80-81 0.4833599116163185 1103 82-83 0.40804919833259845 1103 84-85 0.4689405860885145 1103 86-87 0.5213934313707469 1103 88-89 0.5319193533632927 1103 90-91 0.5782937042604921 1103 92-93 0.6496327845920007 1103 94-95 0.6117032922952568 1103 96-97 0.6107038353805869 1103 98-99 0.5485054431100949 1103 100-101 0.5525958168878944 1103 102-103 0.4779367097797156 1103 104-105 0.5100296838218306 1103 106-107 0.5288593669879234 1103 108-109 0.4630379756769898 1103 110-111 0.42367108143681165 1103 112-113 0.5411244512501128 1103 114-115 0.5338191727754804 1103 116-117 0.5326426520653769 1103 118-119 0.2928993852333761 1103 120-121 0.3587000067375392 1103 122-123 0.36990248082640065 1103 124-125 0.2159366891371235 1104 1 0.2544676670018049 1104 2 0.2536096228374696 1104 3 0.09659035189217136 1104 4 0.22077684809363518 1104 5 0.22674615102804552 1104 6 0.22194239630788104 1104 7 0.17196979187463057 1104 8 0.12231240606663363 1104 9 0.2873766145007437 1104 10-11 0.24747060189592673 1104 12-13 0.28506733153071906 1104 14-15 0.43624863086715493 1104 16-17 0.2964624403911458 1104 18-19 0.47223883557391844 1104 20-21 0.5561419495209066 1104 22-23 0.5615045904612543 1104 24-25 0.7570970114922346 1104 26-27 0.6777557613066563 1104 28-29 0.5033131543175671 1104 30-31 0.4835513578166939 1104 32-33 0.6798129086585121 1104 34-35 0.6093625978230648 1104 36-37 0.5156548067038429 1104 38-39 0.6239193971433394 1104 40-41 0.5796055158504245 1104 42-43 0.45118349159249505 1104 44-45 0.3090356091920228 1104 46-47 0.3151368913939052 1104 48-49 0.20864819197065287 1104 50-51 0.22477858932183636 1104 52-53 0.2725030777462365 1104 54-55 0.2885806882170243 1104 56-57 0.3010658458921682 1104 58-59 0.33496788555786594 1104 60-61 0.3176255634634515 1104 62-63 0.38440305003307884 1104 64-65 0.44948085336051236 1104 66-67 0.44102050236840284 1104 68-69 0.4268614480259032 1104 70-71 0.34623170093227174 1104 72-73 0.3707589436225973 1104 74-75 0.36872205170340067 1104 76-77 0.3428892818350775 1104 78-79 0.386204516554578 1104 80-81 0.289150996819167 1104 82-83 0.32561033532098094 1104 84-85 0.28989105151782724 1104 86-87 0.2990720387707171 1104 88-89 0.2780670748722116 1104 90-91 0.3244330324431566 1104 92-93 0.3202917046059284 1104 94-95 0.33200047286075574 1104 96-97 0.3258171685279798 1104 98-99 0.1663844159942265 1104 100-101 0.23594118495978478 1104 102-103 0.2135302754816415 1104 104-105 0.22550315522925146 1104 106-107 0.259254881181171 1104 108-109 0.21602922802324542 1104 110-111 0.14978406232275532 1104 112-113 0.23507640113601624 1104 114-115 0.19781521215851683 1104 116-117 0.136294678705017 1104 118-119 0.05511980999249744 1104 120-121 0.01356294501121269 1104 122-123 0.0531747623958978 1104 124-125 0.1559751538143921 1105 1 0.45101193185897515 1105 2 0.3926164684052402 1105 3 0.4065013110461031 1105 4 0.38581006665965845 1105 5 0.3227573499592111 1105 6 0.3664723874492637 1105 7 0.3868334825854731 1105 8 0.32593169836653146 1105 9 0.46551176812256045 1105 10-11 0.43167744336664526 1105 12-13 0.4888973470045741 1105 14-15 0.4897529560008991 1105 16-17 0.5425920415135366 1105 18-19 0.6558692173838239 1105 20-21 0.7022458691468216 1105 22-23 0.6012798698724282 1105 24-25 0.8903806056626351 1105 26-27 0.8576582040966301 1105 28-29 0.6583993522119798 1105 30-31 0.660916066544047 1105 32-33 0.8827560006607271 1105 34-35 0.8256820521562176 1105 36-37 0.7277140180420716 1105 38-39 0.7399321849297422 1105 40-41 0.7283658340411705 1105 42-43 0.6484739063486629 1105 44-45 0.5872853177769883 1105 46-47 0.5537823223609024 1105 48-49 0.5622385551140141 1105 50-51 0.5112631771762892 1105 52-53 0.4260631876870278 1105 54-55 0.4269917755286201 1105 56-57 0.43514805216254615 1105 58-59 0.38217599711349237 1105 60-61 0.32576913352122006 1105 62-63 0.46721032489153913 1105 64-65 0.4498125518717373 1105 66-67 0.4552617415555673 1105 68-69 0.5252726052870926 1105 70-71 0.505698242788867 1105 72-73 0.4527313824804011 1105 74-75 0.45677448492526196 1105 76-77 0.5031187739859817 1105 78-79 0.5001534788272508 1105 80-81 0.403510350904547 1105 82-83 0.46797869641681444 1105 84-85 0.5005145472891215 1105 86-87 0.45603969357543406 1105 88-89 0.4051922936520782 1105 90-91 0.35725263591885437 1105 92-93 0.47236232522377364 1105 94-95 0.3675093886869014 1105 96-97 0.3562168280732756 1105 98-99 0.3834299097159999 1105 100-101 0.3869813605132961 1105 102-103 0.31421317095545476 1105 104-105 0.34709205043025904 1105 106-107 0.4851752305694319 1105 108-109 0.3684178695070557 1105 110-111 0.4544788422338293 1105 112-113 0.44911918331145984 1105 114-115 0.4862923643703496 1105 116-117 0.4623972842777029 1105 118-119 0.46755290194974464 1105 120-121 0.41919100249579344 1105 122-123 0.3833778510705095 1105 124-125 0.35668435533469633 1106 1 0.23716198996418214 1106 2 0.30161957638003756 1106 3 0.2959827734290492 1106 4 0.3170393514984937 1106 5 0.2042480615331712 1106 6 0.23021053286549176 1106 7 0.2255651355385666 1106 8 0.2629184901742576 1106 9 0.31817594336488497 1106 10-11 0.3660365975528421 1106 12-13 0.3546161023760419 1106 14-15 0.3578344541624574 1106 16-17 0.4561012225103269 1106 18-19 0.5131151332041384 1106 20-21 0.5116153575594069 1106 22-23 0.39293585199993686 1106 24-25 0.6298918913384881 1106 26-27 0.5141987885304076 1106 28-29 0.4580240154446571 1106 30-31 0.5207059536218992 1106 32-33 0.6909373449649223 1106 34-35 0.7238321205372671 1106 36-37 0.589492046799478 1106 38-39 0.665413771556949 1106 40-41 0.6032808831970513 1106 42-43 0.5223405861870916 1106 44-45 0.4597971630190898 1106 46-47 0.444815028027179 1106 48-49 0.2932764757695665 1106 50-51 0.3360090019152864 1106 52-53 0.3979816498233575 1106 54-55 0.34044759221508514 1106 56-57 0.3262651248183488 1106 58-59 0.3283350278961805 1106 60-61 0.35267694848508313 1106 62-63 0.3775257044624212 1106 64-65 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76-77 -2.2490726661345306 2216 78-79 -2.2720470709999177 2216 80-81 -2.2945477423356913 2216 82-83 -2.322563402109168 2216 84-85 -2.2195398270330244 2216 86-87 -2.165116616579663 2216 88-89 -2.08374895441829 2216 90-91 -2.138552021712737 2216 92-93 -2.1934729184710235 2216 94-95 -2.11750289278762 2216 96-97 -2.229101293753434 2216 98-99 -2.1733393101021115 2216 100-101 -2.167243245672381 2216 102-103 -2.308256918300202 2216 104-105 -2.311991424253595 2216 106-107 -2.272679356447732 2216 108-109 -2.1821644257009787 2216 110-111 -2.227827641395063 2216 112-113 -2.1912710901500922 2216 114-115 -2.2387858854140745 2216 116-117 -2.1362803379671753 2216 118-119 -2.0609355154192137 2216 120-121 -1.8959788898831214 2216 122-123 -1.9257554410541786 2216 124-125 -1.7364922573794708 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 30749.0 3 6327.0 4 4393.0 5 3571.0 6 4124.0 7 4361.0 8 4352.0 9 4744.0 10 5489.0 11 6435.0 12 6834.0 13 6770.0 14 6598.0 15 6566.0 16 6371.0 17 5812.0 18 5530.0 19 5144.0 20 4979.0 21 5127.0 22 5837.0 23 7938.0 24 12681.0 25 18902.0 26 23184.0 27 20814.0 28 22865.0 29 27976.0 30 33421.0 31 40269.0 32 52373.0 33 69883.0 34 99249.0 35 152038.0 36 295250.0 37 619718.0 38 314358.0 39 1715.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.470219172298165 18.988031917619256 12.290343341921494 24.25140556816109 2 15.65270899363548 20.899978378467 39.66516340652888 23.78214922136863 3 18.8606735643785 25.51582981317772 29.54737928790817 26.07611733453561 4 12.170135153135982 16.914788970520426 37.783140184234526 33.13193569210907 5 13.000275632545954 39.16164670465409 34.64373212615158 13.194345536648369 6 31.401802152442944 37.50843078561863 17.806536195404743 13.283230866533685 7 27.625197222977572 33.373021176921235 21.446798573695716 17.554983026405473 8 25.16460145630745 36.85398057198398 20.17921420440026 17.80220376730831 9 25.86320265802508 16.895594266072898 19.849103408155155 37.39209966774686 10-11 23.988608482389083 27.856367732363864 28.411911623045444 19.74311216220161 12-13 24.360257264432462 25.56110798438469 28.03476111781693 22.04387363336592 14-15 21.809605337317098 27.08077956910086 25.412458263799675 25.69715682978237 16-17 22.05058715172074 29.94769521728877 26.19594666740411 21.805770963586376 18-19 21.95507604900096 29.048416374521775 27.599850562477613 21.396657013999654 20-21 22.802941046174773 28.262871942395932 27.399189939620616 21.534997071808675 22-23 22.46456459809488 28.302711600849406 27.06973273250817 22.16299106854754 24-25 22.24826047033748 28.255098241752503 27.53119681996515 21.965444467944874 26-27 22.17142204473318 28.438897143597615 27.35063288092066 22.03904793074854 28-29 22.053104197757467 28.4365727173932 27.41642741473737 22.093895670111962 30-31 22.26094807756472 28.21342871314333 27.48883612203023 22.036787087261718 32-33 22.00461207086386 28.367513322565348 27.524425129896933 22.10344947667386 34-35 22.35239283034427 28.342010709922942 27.36943600039951 21.93616045933328 36-37 22.37183954010025 28.124811932644807 27.240680886415387 22.262667640839563 38-39 22.056914658746656 28.32645045093039 27.520456554856963 22.096178335465986 40-41 22.31928813776327 28.222290506369628 27.453248831700915 22.005172524166188 42-43 22.138438954619023 28.16189798504499 27.448509735249253 22.25115332508673 44-45 22.131739814199822 28.35270693583334 27.363355034431052 22.152198215535787 46-47 22.14587237102136 28.291878914757824 27.138019170310752 22.42422954391007 48-49 22.061185276531063 28.319596741319163 27.332728356545733 22.286489625604037 50-51 22.023527254887302 28.290983728287912 27.35382652042235 22.331662496402444 52-53 22.217852244922206 28.303165126761776 27.296286509367967 22.18269611894805 54-55 22.076493105336972 28.080538411330135 27.425371939997955 22.417596543334938 56-57 22.13084468233673 28.363661782927217 27.27348147146688 22.232012063269174 58-59 22.160074014495105 28.25118733293028 27.225037712720614 22.363700939854002 60-61 22.160957441250854 28.206631244577334 27.50794641000505 22.124464904166757 62-63 22.093447576230858 28.150328820928383 27.45148351618804 22.30474008665272 64-65 21.872220199385158 28.182985328542028 27.368114864370057 22.57667960770276 66-67 21.927916924325704 28.435545552196917 27.03367555574333 22.602861967734047 68-69 21.855717779999345 28.15439706316169 27.383748443082368 22.606136713756598 70-71 21.75285834704937 28.02680593352186 27.53817530785547 22.682160411573296 72-73 21.935754300091514 28.01797621845955 27.43337271488423 22.612896766564706 74-75 21.72921455367241 28.180232756133016 27.360892862583956 22.72965982761062 76-77 21.736662330878662 28.105661788051663 27.406311132050348 22.751364749019327 78-79 21.882175207246423 28.023275613737475 27.374375060013445 22.720174119002657 80-81 21.72733960449051 28.165846561408802 27.35437893190263 22.75243490219806 82-83 21.791841828590623 28.184601250462816 27.375497831995112 22.648059088951445 84-85 21.827837402987573 28.029078311853834 27.30962363775438 22.833460647404213 86-87 21.7294322799704 27.906444368763232 27.514717292274288 22.84940605899208 88-89 21.866381388574723 27.781685443519777 27.4941114023581 22.857821765547396 90-91 21.904499513118406 27.885141520847572 27.481374186492936 22.72898477954109 92-93 21.81281304323668 27.708479338026137 27.57230929087873 22.90639832785845 94-95 21.695770112564507 27.641624849506876 27.57558966932224 23.08701536860638 96-97 21.759723830737247 27.658304550765333 27.487260689025106 23.094710929472317 98-99 21.67179324316364 27.482821059856725 27.50720324549429 23.33818245148534 100-101 21.589253543290955 27.62556931456895 27.581880783576594 23.203296358563502 102-103 21.666502357726596 27.562726538283773 27.567284510958977 23.203486593030657 104-105 21.55262970548753 27.471944669371943 27.60177947954423 23.373646145596293 106-107 21.48196794540463 27.657116131441565 27.607733592472133 23.253182330681668 108-109 21.445055697735604 27.769824514685226 27.31871190424675 23.466407883332415 110-111 21.354314030896973 27.874849152372683 27.41397812430946 23.356858692420886 112-113 21.379418080405447 27.77236426122999 27.555197594776732 23.293020063587836 114-115 21.218990187098772 27.910752595634293 27.35543438838926 23.514822828877673 116-117 21.258719582331047 27.86170095579175 27.409858155483313 23.46972130639389 118-119 21.14506733642051 27.796711823144282 27.37826984881972 23.679950991615488 120-121 21.153235359485954 27.90952411103589 27.34392163185954 23.59331889761862 122-123 21.222760051823542 27.859251805923446 27.284905791324448 23.633082350928564 124-125 21.173841722198276 27.777012867783995 27.21941916959202 23.82972624042571 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2157.0 1 1714.5 2 1177.5 3 896.5 4 630.5 5 513.0 6 438.0 7 428.5 8 444.0 9 437.5 10 418.5 11 401.5 12 418.0 13 439.0 14 466.5 15 537.5 16 666.5 17 755.5 18 930.5 19 1199.5 20 1574.5 21 2176.5 22 2985.0 23 4078.5 24 5694.0 25 7812.5 26 10928.0 27 14586.5 28 17411.0 29 20315.5 30 24580.5 31 29129.0 32 33687.0 33 38524.5 34 44462.0 35 49912.0 36 54444.0 37 58549.0 38 60310.0 39 61238.0 40 62919.5 41 65738.5 42 67525.0 43 69943.5 44 72994.0 45 74730.0 46 74508.5 47 73612.5 48 75288.0 49 75650.0 50 73390.5 51 69983.5 52 66077.0 53 63176.0 54 59578.0 55 54918.0 56 50187.5 57 45084.0 58 40354.0 59 35289.5 60 29243.5 61 22883.5 62 17723.0 63 13747.0 64 10585.0 65 8246.5 66 6165.5 67 4898.5 68 3619.5 69 2238.0 70 1505.0 71 1141.0 72 923.5 73 701.0 74 560.5 75 462.5 76 297.5 77 151.5 78 94.0 79 60.5 80 29.0 81 26.0 82 24.5 83 17.5 84 11.0 85 9.0 86 8.0 87 6.5 88 4.5 89 3.0 90 3.5 91 2.5 92 3.0 93 3.0 94 2.0 95 1.5 96 2.0 97 2.5 98 1.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0626297211057039 2 0.050646601940753204 3 0.04567924057750441 4 0.04542319102269777 5 0.044706252269239175 6 0.004199212698828881 7 4.096792876906225E-4 8 0.0 9 9.729883082652284E-4 10-11 0.0033798541234476356 12-13 0.0024580757261437347 14-15 0.007963141154486476 16-17 0.002944569880276349 18-19 0.004685706852961494 20-21 0.009038549284674359 22-23 0.016438381418586227 24-25 0.00327743430152498 26-27 0.016694430973392866 28-29 0.006810918157856599 30-31 0.006785313202375935 32-33 0.0023044459932597514 34-35 0.01802588865838739 36-37 0.016463986374066892 38-39 0.0287287600493049 40-41 0.006247609137281993 42-43 0.0012034329075912035 44-45 2.0483964384531125E-4 46-47 0.0020483964384531124 48-49 0.0028165451028730297 50-51 0.0016899270617238178 52-53 0.0014850874178785065 54-55 0.005812324894110706 56-57 0.002176421215856432 58-59 0.008014351065447803 60-61 0.015004503911669048 62-63 0.012315983586199338 64-65 0.0022532360822984238 66-67 0.003559088811812283 68-69 0.008756894774387056 70-71 0.0023044459932597514 72-73 0.0028677550138343573 74-75 8.449635308619089E-4 76-77 3.5846937672929466E-4 78-79 0.003046989702199005 80-81 0.0025861005035470543 82-83 0.0015362973288398345 84-85 0.01159904483274075 86-87 0.005735510027668715 88-89 0.001664322106243154 90-91 6.913337979779254E-4 92-93 0.002125211304895104 94-95 0.0 96-97 0.0016131121952818262 98-99 0.025707375302586558 100-101 0.0148252692233044 102-103 0.006375633914685313 104-105 0.013621836315713199 106-107 0.0327743430152498 108-109 9.985932637458924E-4 110-111 0.0016131121952818262 112-113 0.016873665661757516 114-115 0.031903774528907226 116-117 0.04926393434479735 118-119 0.3562929555134382 120-121 0.11576000372808151 122-123 0.018051493613868055 124-125 0.32047162279598945 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1952747.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.552292111866 #Duplication Level Percentage of deduplicated Percentage of total 1 87.4676233532667 64.33444183210145 2 8.20879398908597 12.07551226742762 3 2.0617533908931995 4.549400630088205 4 0.7758496717831536 2.2826208677555946 5 0.382255417043687 1.405788104787021 6 0.214778499941681 0.9478470580235349 7 0.15554858444265618 0.8008668444359452 8 0.09612290295982819 0.5656047869713465 9 0.07684824405830464 0.5087128045734354 >10 0.49032561769081184 7.061191192234342 >50 0.04702915972986073 2.406696272591374 >100 0.02292606745385172 2.7509593241006693 >500 0.0 0.0 >1k 1.4510165034550343E-4 0.3103580149094331 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2908 0.1489184210755413 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 5.120991096132781E-5 0.0 0.0 0.0 6 0.0 5.120991096132781E-5 0.0 0.0 0.0 7 0.0 5.120991096132781E-5 0.0 0.0 0.0 8 0.0 5.120991096132781E-5 0.0 0.0 0.0 9 0.0 5.120991096132781E-5 0.0 0.0 0.0 10-11 0.0 1.0241982192265562E-4 0.0 0.0 0.0 12-13 0.0 1.2802477740331953E-4 0.0 0.0 0.0 14-15 0.0 1.5362973288398344E-4 0.0 0.0 0.0 16-17 0.0 2.0483964384531125E-4 0.0 0.0 0.0 18-19 0.0 2.0483964384531125E-4 0.0 0.0 0.0 20-21 0.0 2.0483964384531125E-4 0.0 7.681486644199172E-5 0.0 22-23 0.0 2.0483964384531125E-4 0.0 2.0483964384531125E-4 5.120991096132781E-5 24-25 0.0 2.0483964384531125E-4 0.0 5.37704065093942E-4 1.0241982192265562E-4 26-27 0.0 2.0483964384531125E-4 0.0 0.001075408130187884 1.0241982192265562E-4 28-29 0.0 2.0483964384531125E-4 0.0 0.003584693767292947 1.0241982192265562E-4 30-31 0.0 2.0483964384531125E-4 0.0 0.010907711034762825 1.0241982192265562E-4 32-33 0.0 2.0483964384531125E-4 0.0 0.02537451088133793 1.0241982192265562E-4 34-35 0.0 2.0483964384531125E-4 0.0 0.05113309609488582 1.0241982192265562E-4 36-37 0.0 2.0483964384531125E-4 0.0 0.09082077708991487 1.0241982192265562E-4 38-39 0.0 2.0483964384531125E-4 0.0 0.1462042957945909 1.0241982192265562E-4 40-41 0.0 2.0483964384531125E-4 0.0 0.20051240636907905 1.0241982192265562E-4 42-43 0.0 2.5604955480663905E-4 0.0 0.2544620475668379 1.0241982192265562E-4 44-45 0.0 2.5604955480663905E-4 0.0 0.3274105657312494 1.0241982192265562E-4 46-47 0.0 2.5604955480663905E-4 0.0 0.4186410221088549 1.2802477740331953E-4 48-49 0.0 2.5604955480663905E-4 0.0 0.5171176808874882 1.5362973288398344E-4 50-51 0.0 2.5604955480663905E-4 0.0 0.6115231517446961 1.5362973288398344E-4 52-53 0.0 2.5604955480663905E-4 0.0 0.7037009914750861 1.5362973288398344E-4 54-55 0.0 2.5604955480663905E-4 0.0 0.8141095595077089 1.5362973288398344E-4 56-57 0.0 3.072594657679669E-4 0.0 0.9555001236719349 1.5362973288398344E-4 58-59 0.0 3.5846937672929466E-4 0.0 1.110614943973797 1.5362973288398344E-4 60-61 0.0 3.5846937672929466E-4 0.0 1.2732832261424547 1.5362973288398344E-4 62-63 0.0 3.5846937672929466E-4 0.0 1.4395362020784055 1.5362973288398344E-4 64-65 0.0 4.864941541326142E-4 0.0 1.6184892359327656 1.5362973288398344E-4 66-67 0.0 5.633090205746059E-4 0.0 1.8240202135760546 1.5362973288398344E-4 68-69 0.0 5.889139760552699E-4 0.0 2.0646043752723724 1.5362973288398344E-4 70-71 0.0 6.145189315359338E-4 0.0 2.3314080113808906 1.5362973288398344E-4 72-73 0.0 6.145189315359338E-4 0.0 2.600285648883342 1.5362973288398344E-4 74-75 0.0 6.145189315359338E-4 0.0 2.8758717847217277 1.5362973288398344E-4 76-77 0.0 6.657288424972615E-4 0.0 3.1853588816165126 1.5362973288398344E-4 78-79 0.0 6.657288424972615E-4 0.0 3.535685882502956 1.5362973288398344E-4 80-81 0.0 6.913337979779254E-4 0.0 3.9207075980656994 1.5362973288398344E-4 82-83 0.0 8.449635308619089E-4 0.0 4.319427964810598 1.5362973288398344E-4 84-85 0.0 9.729883082652284E-4 0.0 4.71561344096291 1.5362973288398344E-4 86-87 0.0 0.0010241982192265562 0.0 5.1319500170785055 1.5362973288398344E-4 88-89 0.0 0.0010241982192265562 0.0 5.6045918902960805 1.5362973288398344E-4 90-91 0.0 0.0010241982192265562 0.0 6.1232458685124085 1.5362973288398344E-4 92-93 0.0 0.0010241982192265562 0.0 6.6627678854454775 1.5362973288398344E-4 94-95 0.0 0.0010241982192265562 0.0 7.1923295746965685 2.0483964384531125E-4 96-97 0.0 0.0010241982192265562 0.0 7.7244005495847645 2.0483964384531125E-4 98-99 0.0 0.001075408130187884 0.0 8.311829438222155 2.0483964384531125E-4 100-101 0.0 0.001075408130187884 0.0 8.960249330814488 2.0483964384531125E-4 102-103 0.0 0.0011010130856685479 0.0 9.633147560846336 2.0483964384531125E-4 104-105 0.0 0.0011266180411492119 0.0 10.30166734349099 2.0483964384531125E-4 106-107 0.0 0.0011266180411492119 0.0 10.974796018122163 2.0483964384531125E-4 108-109 0.0 0.0011266180411492119 0.0 11.670380238709878 2.3044459932597514E-4 110-111 0.0 0.001152222996629876 0.0 12.40977453812501 2.5604955480663905E-4 112-113 0.0 0.0011778279521105397 0.0 13.181405476490298 2.5604955480663905E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1845 0.0 52.89201 1 GTATCAA 3250 0.0 41.744003 1 TCAACGC 3230 0.0 39.050934 4 GTACATG 7995 0.0 38.70147 1 ATCAACG 3385 0.0 37.61432 3 CAACGCA 3355 0.0 37.59598 5 TACATGG 8190 0.0 36.83362 2 ACATGGG 8025 0.0 35.06826 3 AACGCAG 3650 0.0 35.031162 6 TATCAAC 4130 0.0 31.69516 2 CATGGGG 3785 0.0 28.76625 4 ACGCAGA 4380 0.0 28.377232 7 CGCAGAG 4510 0.0 27.163675 8 CATGGGA 5135 0.0 26.185812 4 TGTACCG 200 1.8276296E-6 23.798965 5 CGTAGAC 215 3.4256627E-6 22.13857 3 GAGTACA 5690 0.0 21.647148 1 ATGGGGG 2290 0.0 21.30475 5 AGTACAT 5495 0.0 21.223093 2 GCAGAGT 5610 0.0 21.097576 9 >>END_MODULE