FastQCFastQC Report
Thu 26 May 2016
SRR1364198_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364198_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1952747
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73260.37516380770268754No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71010.3636415777363888No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39470.20212551856436087No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42500.076.066531
GTATCAA71850.048.805771
ATCAACG91250.037.9601673
TCAACGC91350.037.9176454
CAACGCA92950.037.257315
AACGCAG96000.036.4455076
TATCAAC95350.036.2664152
GTACATG78550.035.4717251
ACATGGG77650.034.8744963
TACATGG79500.034.7376172
TGGTATC18300.032.1983262
ACGCAGA111400.031.2430177
CGCAGAG111900.030.9431178
GTGGTAT20200.030.652411
CATGGGG38350.027.7788684
GCAGAGT128400.026.8741139
GAGTACT88550.026.60725212-13
CAGAGTA125250.025.9349910-11
CATGGGA47750.025.5509684
GTACTTT98050.023.99895314-15
Produced by FastQC (version 0.11.5)