FastQCFastQC Report
Thu 26 May 2016
SRR1364197_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364197_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2446236
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23400.046.782661
GTATCAA33300.041.807541
GTACATG81500.035.843211
ACCGTAC700.002797950533.979278
AACGCAG39750.033.959296
TACATGG85250.033.4954222
CAACGCA41550.032.6432575
ATCAACG42050.032.255113
TCAACGC42100.032.2161454
TATCAAC44000.031.637472
ACATGGG87150.031.6040923
CATGGGG66850.028.1194294
ACGCAGA49100.027.00757
CGCAGAG51900.024.747918
GAGTACA69150.023.4023631
AGTACAT67550.022.9854892
ATGGGGA36850.022.1163185
CAGAGTA54400.019.78601810-11
AGAGTAC51000.019.35604910-11
GCAGAGT66100.019.2526769
Produced by FastQC (version 0.11.5)