##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364197_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2446236 Sequences flagged as poor quality 0 Sequence length 125 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.684408209183413 31.0 31.0 34.0 27.0 34.0 2 30.71350965319781 31.0 31.0 34.0 27.0 34.0 3 30.8108449062151 33.0 31.0 34.0 27.0 34.0 4 34.24849319525998 37.0 35.0 37.0 30.0 37.0 5 34.33461816439624 37.0 35.0 37.0 30.0 37.0 6 34.378420561221404 37.0 35.0 37.0 30.0 37.0 7 34.27980456505423 37.0 35.0 37.0 30.0 37.0 8 34.23053785489217 37.0 35.0 37.0 30.0 37.0 9 35.70747957269863 39.0 37.0 39.0 30.0 39.0 10-11 35.649527069342454 39.0 37.0 39.0 30.0 39.0 12-13 35.59967435684864 39.0 37.0 39.0 30.0 39.0 14-15 36.85479916900904 40.0 37.0 41.0 30.0 41.0 16-17 36.86324581929135 40.0 37.0 41.0 30.0 41.0 18-19 36.73792205657999 40.0 37.0 41.0 29.5 41.0 20-21 36.67302541537285 40.0 37.0 41.0 29.0 41.0 22-23 36.684871574124486 40.0 37.0 41.0 29.0 41.0 24-25 36.533967082489184 40.0 37.0 41.0 28.0 41.0 26-27 36.49309326655319 40.0 36.5 41.0 28.5 41.0 28-29 36.55190995472228 40.0 36.0 41.0 29.0 41.0 30-31 36.428952276068216 40.0 36.0 41.0 29.0 41.0 32-33 36.23477804267454 40.0 36.0 41.0 27.5 41.0 34-35 36.178278179210835 40.0 36.0 41.0 28.0 41.0 36-37 36.19240253188981 40.0 36.0 41.0 28.0 41.0 38-39 36.02611134003424 40.0 35.5 41.0 27.5 41.0 40-41 35.99820969849189 40.0 35.0 41.0 27.5 41.0 42-43 35.91446614308677 40.0 35.0 41.0 27.0 41.0 44-45 35.88567619804467 40.0 35.0 41.0 27.5 41.0 46-47 35.76625517734184 39.5 35.0 41.0 27.5 41.0 48-49 35.6409175157262 39.0 35.0 41.0 27.0 41.0 50-51 35.48522076365486 39.0 35.0 41.0 27.0 41.0 52-53 35.293332082431945 39.0 35.0 41.0 27.0 41.0 54-55 35.063218961702795 39.0 34.0 41.0 26.0 41.0 56-57 34.83128671967872 38.0 34.0 40.0 26.0 41.0 58-59 34.562755801157365 38.0 34.0 40.0 26.0 41.0 60-61 34.28685601062203 37.0 34.0 40.0 26.0 41.0 62-63 33.97960192720572 37.0 33.0 40.0 25.5 41.0 64-65 33.658071420746 36.0 33.0 39.5 25.0 41.0 66-67 33.344170595151084 36.0 33.0 39.0 24.0 41.0 68-69 33.120351225311055 35.0 33.0 39.0 24.0 41.0 70-71 32.963903319221856 35.0 33.0 38.5 24.0 40.5 72-73 32.67587714349719 35.0 33.0 37.0 24.0 40.0 74-75 32.374254364664736 35.0 33.0 37.0 24.0 39.0 76-77 32.07459194452211 35.0 33.0 36.5 23.0 39.0 78-79 31.777009863316543 35.0 32.0 36.0 23.0 38.5 80-81 31.519349114312764 35.0 32.0 35.5 22.0 37.0 82-83 31.28266365142202 35.0 32.0 35.0 20.5 37.0 84-85 31.075353727113818 35.0 32.0 35.0 20.0 36.5 86-87 30.894932050709745 35.0 32.0 35.0 20.0 36.0 88-89 30.754809225275075 35.0 32.0 35.0 19.5 36.0 90-91 30.626471035501073 35.0 32.0 35.0 19.0 35.5 92-93 30.51321806236193 35.0 32.0 35.0 18.0 35.0 94-95 30.402122076529004 35.0 31.5 35.0 17.5 35.0 96-97 30.31720999118646 35.0 31.0 35.0 17.0 35.0 98-99 30.239979094412803 35.0 31.0 35.0 15.0 35.0 100-101 30.13916972851352 35.0 31.0 35.0 12.5 35.0 102-103 29.145676664066755 33.5 29.5 34.5 9.0 35.0 104-105 29.850970020881057 34.0 31.0 35.0 8.0 35.0 106-107 29.97648346275666 34.5 31.0 35.0 7.0 35.0 108-109 29.93336763092359 35.0 31.0 35.0 7.0 35.0 110-111 29.860292506528396 35.0 31.0 35.0 3.5 35.0 112-113 30.044600970634068 35.0 31.5 35.0 2.0 35.0 114-115 30.04088403571855 35.0 32.0 35.0 2.0 35.0 116-117 29.950402168883134 35.0 32.0 35.0 2.0 35.0 118-119 29.69233283297278 35.0 31.0 35.0 2.0 35.0 120-121 29.626096378272578 35.0 31.0 35.0 2.0 35.0 122-123 29.470047248098712 35.0 31.0 35.0 2.0 35.0 124-125 28.540314998225845 34.5 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8618786339626574 1101 2 0.824477671927724 1101 3 0.829687644265821 1101 4 0.847809877161076 1101 5 0.8009829124157903 1101 6 0.8007674138445822 1101 7 0.9047355161311899 1101 8 0.8461428959092387 1101 9 0.9898467970020448 1101 10-11 0.9992105370024831 1101 12-13 0.963188782532356 1101 14-15 1.1939482226090803 1101 16-17 1.1330584463402573 1101 18-19 1.244150036692801 1101 20-21 1.3113249718937752 1101 22-23 1.1951910136621535 1101 24-25 1.4240485348826653 1101 26-27 1.4654275021678913 1101 28-29 1.2920989342366127 1101 30-31 1.4185497133453353 1101 32-33 1.5630848896301686 1101 34-35 1.5005519125891524 1101 36-37 1.479733123698061 1101 38-39 1.5328599227662565 1101 40-41 1.4230117432206697 1101 42-43 1.328390550727363 1101 44-45 1.257145093010216 1101 46-47 1.1700558100970326 1101 48-49 1.1471457016532796 1101 50-51 1.098778198306924 1101 52-53 1.0841766885653854 1101 54-55 1.1381715945673392 1101 56-57 1.1511307554138597 1101 58-59 1.1022074524813874 1101 60-61 0.9574619094553469 1101 62-63 1.015740865714072 1101 64-65 0.9310069246050574 1101 66-67 0.9601754706313983 1101 68-69 0.9293975324595749 1101 70-71 0.9062980006194508 1101 72-73 0.887448991451528 1101 74-75 0.835282981183866 1101 76-77 0.8654755756625505 1101 78-79 0.8619836437109569 1101 80-81 0.9105978365363185 1101 82-83 0.8568473265888592 1101 84-85 0.9033562040150791 1101 86-87 0.9039682987817024 1101 88-89 1.021114038743093 1101 90-91 1.011126186811424 1101 92-93 0.9425508994525487 1101 94-95 1.0081389156418972 1101 96-97 0.9571835929909511 1101 98-99 0.8526911202927998 1101 100-101 0.8896527990340957 1101 102-103 0.8067249136835244 1101 104-105 0.9204120892537055 1101 106-107 0.9050530245455874 1101 108-109 0.9563951671173214 1101 110-111 0.9076195246956225 1101 112-113 0.9448967919542497 1101 114-115 0.9338408437352577 1101 116-117 0.9825683353429611 1101 118-119 0.8541417155610453 1101 120-121 0.8640196458676677 1101 122-123 0.8859556911165285 1101 124-125 0.8172129850356633 1102 1 0.30598514320513104 1102 2 0.3251980115673234 1102 3 0.35388386670496885 1102 4 0.40817384313513827 1102 5 0.3739085852647648 1102 6 0.28353147898349107 1102 7 0.4302391650876345 1102 8 0.49588041895732005 1102 9 0.450423967395146 1102 10-11 0.5409257789492088 1102 12-13 0.5656878141256598 1102 14-15 0.6185734879525171 1102 16-17 0.6220826797495107 1102 18-19 0.648284590993299 1102 20-21 0.6982088715513513 1102 22-23 0.7540403140851524 1102 24-25 0.9517166890220139 1102 26-27 0.9099483345328139 1102 28-29 0.7078950420164034 1102 30-31 0.8984005787135629 1102 32-33 0.9905294580306645 1102 34-35 0.902931326586895 1102 36-37 0.7400698968119954 1102 38-39 0.7905053774525683 1102 40-41 0.7697628549515656 1102 42-43 0.7377634105485527 1102 44-45 0.6094772487609958 1102 46-47 0.64018805571596 1102 48-49 0.6115999851097911 1102 50-51 0.5884073372771539 1102 52-53 0.6026645021354753 1102 54-55 0.6520450664215645 1102 56-57 0.628369324341179 1102 58-59 0.6488859724460596 1102 60-61 0.6343299272430443 1102 62-63 0.6277799428612312 1102 64-65 0.5571581664898133 1102 66-67 0.5663074218546953 1102 68-69 0.5736331884195423 1102 70-71 0.523380384914212 1102 72-73 0.4985590179471302 1102 74-75 0.5474255616390948 1102 76-77 0.5390363683615291 1102 78-79 0.5917601354446376 1102 80-81 0.6018560679932676 1102 82-83 0.6172742002621447 1102 84-85 0.5692484552120334 1102 86-87 0.46666310347343654 1102 88-89 0.5085860324213343 1102 90-91 0.5225356446586176 1102 92-93 0.5458406932206614 1102 94-95 0.45268562383384037 1102 96-97 0.502477988627696 1102 98-99 0.5495016534587904 1102 100-101 0.48511972600848097 1102 102-103 0.42623280813494446 1102 104-105 0.4618585015005614 1102 106-107 0.40430463984796106 1102 108-109 0.4894185328341756 1102 110-111 0.49507215023986717 1102 112-113 0.5280661159289011 1102 114-115 0.4781076436546847 1102 116-117 0.5495816916081608 1102 118-119 0.33565824045297177 1102 120-121 0.40450268712953985 1102 122-123 0.42749933139604934 1102 124-125 0.27459247650594065 1103 1 0.5233466917102589 1103 2 0.1537766706043726 1103 3 0.4533152764416286 1103 4 0.5183753942990421 1103 5 0.48256845976964513 1103 6 0.49107988221348364 1103 7 0.43112771548113926 1103 8 0.4229429348974918 1103 9 0.5676962948408217 1103 10-11 0.5828824433407007 1103 12-13 0.6127579623169481 1103 14-15 0.733404973762795 1103 16-17 0.6654617348495862 1103 18-19 0.8088747383764172 1103 20-21 0.9467324928780769 1103 22-23 0.925835032151582 1103 24-25 1.1088740436981297 1103 26-27 1.0250247609936736 1103 28-29 0.8819073294575475 1103 30-31 1.0480884148115166 1103 32-33 1.25800014640906 1103 34-35 1.1528897833055964 1103 36-37 0.97004885358443 1103 38-39 1.1916298058389856 1103 40-41 1.1375598356078456 1103 42-43 1.0327882384239473 1103 44-45 1.008308817438234 1103 46-47 0.9407009157589599 1103 48-49 0.8111200668757519 1103 50-51 0.7538123692629242 1103 52-53 0.7740668466552663 1103 54-55 0.841062311238943 1103 56-57 0.8638547972962414 1103 58-59 0.7920814904274565 1103 60-61 0.8041452086114731 1103 62-63 0.7685261641429264 1103 64-65 0.8037792519414495 1103 66-67 0.8286827078262036 1103 68-69 0.8176178310946085 1103 70-71 0.8082153230260687 1103 72-73 0.7718699596348628 1103 74-75 0.7816050016751888 1103 76-77 0.7533960396227783 1103 78-79 0.7500738940230534 1103 80-81 0.7088299441139085 1103 82-83 0.621640820048956 1103 84-85 0.6793685303017902 1103 86-87 0.6594228306039902 1103 88-89 0.7125776353346218 1103 90-91 0.7051650700978342 1103 92-93 0.7079214940609262 1103 94-95 0.7220759046239671 1103 96-97 0.7894731407646844 1103 98-99 0.7350219056437126 1103 100-101 0.8000831693850259 1103 102-103 0.7654232683041933 1103 104-105 0.7567539412707838 1103 106-107 0.7335753696891132 1103 108-109 0.7094595314775596 1103 110-111 0.7255079733004841 1103 112-113 0.7378679219013549 1103 114-115 0.7451063125127853 1103 116-117 0.7642680358081932 1103 118-119 0.8151489996167243 1103 120-121 0.8858475577092229 1103 122-123 0.9619298538999885 1103 124-125 0.7762710812841718 1104 1 0.7536813323824063 1104 2 0.703794625171902 1104 3 0.7007299866481915 1104 4 0.7315515279067739 1104 5 0.6724036555919852 1104 6 0.7041795374801936 1104 7 0.7354025678632468 1104 8 0.8139527624692704 1104 9 0.844116727838383 1104 10-11 0.9056213573398608 1104 12-13 0.8496680260269756 1104 14-15 1.0215993977990223 1104 16-17 0.9693244228512654 1104 18-19 1.1321872399332733 1104 20-21 1.1729128482581714 1104 22-23 1.1780637495517254 1104 24-25 1.3973653548349674 1104 26-27 1.3788251118004524 1104 28-29 1.178479245547166 1104 30-31 1.1862953776571743 1104 32-33 1.3817239796910386 1104 34-35 1.3954669760508978 1104 36-37 1.2742470526979304 1104 38-39 1.2751107780557192 1104 40-41 1.2584233929296573 1104 42-43 1.1365663034561422 1104 44-45 1.112218847842044 1104 46-47 1.0458072059590648 1104 48-49 1.0352499210813093 1104 50-51 1.0100865320105115 1104 52-53 1.0755270092063753 1104 54-55 1.0742999102024697 1104 56-57 1.0285022398409254 1104 58-59 1.0018377897098034 1104 60-61 0.902034931181916 1104 62-63 0.8970531241225714 1104 64-65 0.9438429311807965 1104 66-67 0.9958769560951879 1104 68-69 0.9508008736423221 1104 70-71 0.9014338842704532 1104 72-73 0.9252060596229015 1104 74-75 0.9054141996196137 1104 76-77 0.8052300224272528 1104 78-79 0.8035833682410995 1104 80-81 0.7532187989035819 1104 82-83 0.7886653535730623 1104 84-85 0.7999943610328977 1104 86-87 0.8908040980145309 1104 88-89 0.8745114262225435 1104 90-91 0.8813550045125851 1104 92-93 0.8438013252460088 1104 94-95 0.9586269119689668 1104 96-97 0.9226250112163896 1104 98-99 0.8344245069428773 1104 100-101 0.9282501983617095 1104 102-103 0.9124606107259261 1104 104-105 0.9117479699426774 1104 106-107 0.929696098552192 1104 108-109 0.8955734227711218 1104 110-111 0.9245397421094665 1104 112-113 0.9660725743034462 1104 114-115 1.0074822293782404 1104 116-117 0.9415796059106327 1104 118-119 0.8550333640852088 1104 120-121 1.0201309297645267 1104 122-123 1.040381203179443 1104 124-125 1.0904507184815593 1105 1 0.4102127460602034 1105 2 0.4203223344261495 1105 3 0.4221610735725747 1105 4 0.40815950520845945 1105 5 0.4103829673001229 1105 6 0.3087883885606999 1105 7 0.34361201896070526 1105 8 0.28844581293747495 1105 9 0.3991789139798456 1105 10-11 0.4409356851531001 1105 12-13 0.4385253940921956 1105 14-15 0.4278855802991899 1105 16-17 0.5778912345386402 1105 18-19 0.7246092850740808 1105 20-21 0.7728195316694908 1105 22-23 0.761009198177355 1105 24-25 1.0130523842029149 1105 26-27 0.9372972779579669 1105 28-29 0.8521886775410934 1105 30-31 0.8590292837641584 1105 32-33 1.0556229934505623 1105 34-35 1.0606257097692833 1105 36-37 0.8807855679051642 1105 38-39 0.9324677964432837 1105 40-41 0.8402637439030229 1105 42-43 0.85105844858316 1105 44-45 0.7434254184594877 1105 46-47 0.7233317373827077 1105 48-49 0.8098550594323868 1105 50-51 0.6638072350868427 1105 52-53 0.6214973688344756 1105 54-55 0.6611001188462353 1105 56-57 0.7521642867758089 1105 58-59 0.7325340884424989 1105 60-61 0.7123262932303476 1105 62-63 0.6803923131029919 1105 64-65 0.6639542133930831 1105 66-67 0.7183350653773388 1105 68-69 0.7148272248848002 1105 70-71 0.8083623191265943 1105 72-73 0.7240307357350204 1105 74-75 0.6180294229730947 1105 76-77 0.6605685452759005 1105 78-79 0.6757041341099068 1105 80-81 0.5740281074720954 1105 82-83 0.6536443064670756 1105 84-85 0.652816409163826 1105 86-87 0.5576007517406332 1105 88-89 0.566083073594644 1105 90-91 0.48135907439495895 1105 92-93 0.5253218166884572 1105 94-95 0.6105527138603826 1105 96-97 0.6500458852277831 1105 98-99 0.5406375561485888 1105 100-101 0.45910551321279414 1105 102-103 0.5010793923194257 1105 104-105 0.4878049350064728 1105 106-107 0.585635095168378 1105 108-109 0.5009599120905861 1105 110-111 0.5006320071734329 1105 112-113 0.5477970582174443 1105 114-115 0.5410778624775148 1105 116-117 0.644225693923083 1105 118-119 0.5486931102906638 1105 120-121 0.6287602432168988 1105 122-123 0.5802288816022525 1105 124-125 0.5942923967349323 1106 1 0.5043759225659379 1106 2 0.6132929944548202 1106 3 0.5771079439581612 1106 4 0.5790022277022544 1106 5 0.5488731875395558 1106 6 0.5536530039699343 1106 7 0.44442505198433935 1106 8 0.47914638567971224 1106 9 0.5595974960628354 1106 10-11 0.6747966044812159 1106 12-13 0.6697865883299556 1106 14-15 0.7334010000384552 1106 16-17 0.7925864050827585 1106 18-19 0.7916396153380632 1106 20-21 0.8523157568339101 1106 22-23 0.7348397494503729 1106 24-25 0.9074356042870377 1106 26-27 0.8525425022785598 1106 28-29 0.828973486948378 1106 30-31 0.8665552320521286 1106 32-33 0.9896095061611945 1106 34-35 1.007531748651104 1106 36-37 0.9301809652787156 1106 38-39 1.015725961323426 1106 40-41 0.9008795155961522 1106 42-43 0.9370300566998111 1106 44-45 0.8549936089355157 1106 46-47 0.7524453068470507 1106 48-49 0.7007246715438598 1106 50-51 0.7053180134154857 1106 52-53 0.7000976412063267 1106 54-55 0.6717182490357914 1106 56-57 0.6553767657872527 1106 58-59 0.6453866112475026 1106 60-61 0.6003581238290678 1106 62-63 0.5403344513241208 1106 64-65 0.5221231346995694 1106 66-67 0.5505066668184782 1106 68-69 0.5970463272714 1106 70-71 0.5764732269380701 1106 72-73 0.5320636779993038 1106 74-75 0.626857509548401 1106 76-77 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-3.6672499556257065 2216 122-123 -3.6792398052850572 2216 124-125 -3.514250019163466 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 51411.0 3 10092.0 4 6949.0 5 6444.0 6 7739.0 7 8155.0 8 7753.0 9 8244.0 10 9121.0 11 9671.0 12 10072.0 13 9824.0 14 9721.0 15 9271.0 16 8501.0 17 7941.0 18 7537.0 19 7252.0 20 7705.0 21 8006.0 22 9376.0 23 12593.0 24 18439.0 25 24230.0 26 27712.0 27 29736.0 28 35276.0 29 41844.0 30 48978.0 31 60313.0 32 76591.0 33 101655.0 34 143457.0 35 231769.0 36 458412.0 37 689461.0 38 224564.0 39 421.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.76200682773668 17.64261469754992 12.122504943278082 25.47287353143532 2 16.735424549079305 19.899568639207413 37.644582755465834 25.720424056247452 3 18.61874266149016 23.809755560908556 29.223086200472853 28.348415577128428 4 13.165458563788976 15.897963156542977 35.470293101953416 35.46628517771463 5 14.526917876735354 37.45179287561069 33.40039048281965 14.620898764834298 6 33.8948779899514 35.308837296769156 16.612458147834726 14.183826565444727 7 29.400623899580864 32.176286174586416 20.52511888716787 17.89797103866485 8 26.795370520260516 34.6240918701221 19.465660713030143 19.114876896587248 9 26.98904550752163 15.594362056257616 18.80307953811016 38.613512898110585 10-11 25.720777566969716 25.516496786982607 27.238922663147658 21.523802982900015 12-13 25.64893859476099 23.329023871014556 27.087474590354095 23.93456294387036 14-15 23.071521871109777 24.833332311271096 24.153129452358613 27.942016365260514 16-17 24.137279766883417 27.703014277900284 24.701204909903613 23.45850104531269 18-19 23.98138101946522 26.522930731538207 25.923733476799455 23.571954772197117 20-21 24.564694973724297 26.023186559530593 25.70963202569434 23.702486441050766 22-23 24.546780362036017 25.670180396543675 25.528735801366732 24.254303440053576 24-25 24.102980142399772 25.946230011632604 25.82753287166748 24.123256974300137 26-27 24.23390572899237 26.182977036643774 25.561818138330462 24.021299096033395 28-29 24.08532615550094 26.20292434801503 25.62206938167032 24.089680114813703 30-31 24.13018740148692 25.999746122179822 25.545157648519034 24.324908827814216 32-33 24.048607524146476 26.24637468167002 25.594047334102022 24.11097046008149 34-35 24.49901003053215 26.091710935048397 25.30173880956299 24.10754022485646 36-37 24.705320774581306 26.036664332769448 25.075541608958073 24.18247328369117 38-39 24.212099936906657 26.062251822980453 25.33234406683396 24.393304173278928 40-41 24.424514188077236 25.86166755005089 25.52302444047847 24.190793821393406 42-43 24.431801690542503 26.106523718245096 25.251318679566694 24.21035591164571 44-45 24.3077914241845 25.988113127639778 25.412737488083692 24.29135796009203 46-47 24.38733443287287 26.23307281100641 25.06441177258132 24.315180983539406 48-49 24.445096263712905 26.067015651157227 25.128886472388185 24.359001612741682 50-51 24.204736638028212 26.112215891960815 25.28077887765303 24.40226859235794 52-53 24.474049225141638 25.769247592888366 25.34648794512607 24.410215236843925 54-55 24.194079189430944 25.763628722480505 25.2119101388178 24.830381949270752 56-57 24.1482068038802 26.056726391495577 25.222767248135142 24.57229955648908 58-59 24.42457608600334 25.59650897602231 25.313153542382405 24.665761395591947 60-61 24.28871939439905 25.75476268840703 25.406599382215074 24.54991853497885 62-63 23.990318620812307 25.89327383319041 25.50838994943646 24.60801759656082 64-65 23.90941668765187 25.842562734857133 25.404346716987547 24.843673860503447 66-67 24.072774590541748 25.808047287785573 25.282277689009387 24.83690043266329 68-69 23.867562556597854 25.58490751169774 25.709049728432515 24.838480203271892 70-71 23.9569106166331 25.516490874922482 25.62993347562374 24.89666503282068 72-73 23.948970402048282 25.703074506091 25.39585691632031 24.95209817554041 74-75 23.762630015086625 25.8549104501194 25.51164425407258 24.870815280721395 76-77 24.043185645500557 25.54884977063681 25.487878385899602 24.92008619796303 78-79 23.977615495983187 25.68958690909596 25.61577724074482 24.717020354176032 80-81 24.0626457637602 25.978868163947922 25.54754029467326 24.41094577761862 82-83 24.056498392298074 25.8514708668795 25.477849565048714 24.61418117577371 84-85 24.055306378308366 25.622516674070095 25.471797430720354 24.85037951690118 86-87 23.934902334491717 25.189400094886572 25.789135549002207 25.086562021619503 88-89 23.861515477502778 25.503852027212233 25.689038764326167 24.94559373095883 90-91 23.930642997972782 25.6962292326956 25.642247833692323 24.73087993563929 92-93 23.72163356026553 25.24343583266716 25.834030509220813 25.200900097846496 94-95 23.82932390824107 25.240655439622344 25.852350304713035 25.077670347423552 96-97 23.788050204552587 25.309986846905957 25.827464518838973 25.074498429702487 98-99 23.60914445532551 25.208749465885138 25.849707744614385 25.33239833417497 100-101 23.42087989207972 25.326227232371835 25.867402707287912 25.38549016826054 102-103 23.48120845126045 24.945289858479285 26.084135673792236 25.48936601646803 104-105 23.179011153202136 25.32230094613841 25.982980833762113 25.515707066897342 106-107 23.411882187351 25.320248854808252 25.976674155428753 25.291194802411997 108-109 23.26783590732249 25.369562542438217 25.774189631849854 25.588411918389436 110-111 22.99325134050326 25.535858934886278 26.07725198722339 25.393637737387063 112-113 23.065703099973486 25.425972675684278 26.286187157927316 25.222137066414916 114-115 22.857733456173257 25.604232170729503 26.079419037489487 25.458615335607753 116-117 22.83851409647604 25.41971021973296 26.418630256436582 25.323145427354422 118-119 22.75049594678503 25.627674476755885 26.064042006810638 25.55778756964845 120-121 22.982297996515054 25.67041183939788 26.183351460637827 25.163938703449233 122-123 22.79807935862501 25.69134850702986 26.140904685299432 25.369667449045703 124-125 22.800112119967544 25.793333865089824 26.061494972543116 25.345059042399516 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 993.0 1 824.5 2 555.5 3 398.0 4 314.5 5 260.0 6 224.0 7 223.5 8 226.0 9 226.5 10 234.0 11 234.5 12 248.5 13 271.5 14 300.0 15 361.0 16 422.0 17 481.5 18 580.0 19 718.0 20 892.5 21 1239.5 22 1725.0 23 2244.0 24 3153.5 25 4584.0 26 6347.5 27 8329.5 28 10648.0 29 13228.0 30 16423.0 31 21641.0 32 27091.0 33 30410.5 34 32851.0 35 34905.0 36 38007.0 37 42443.0 38 46631.0 39 49951.5 40 54573.0 41 61660.5 42 66956.5 43 71559.5 44 77178.0 45 81645.0 46 84539.5 47 88581.5 48 92898.0 49 93821.0 50 94185.0 51 93821.5 52 93827.0 53 97651.0 54 101732.5 55 101774.5 56 96383.0 57 89881.5 58 86260.0 59 79380.5 60 68534.0 61 57776.5 62 48864.0 63 39430.5 64 30727.5 65 24395.5 66 17622.0 67 14450.0 68 10792.5 69 6378.5 70 4931.0 71 3682.5 72 2791.5 73 2267.0 74 1673.5 75 1230.5 76 808.0 77 442.5 78 241.5 79 139.0 80 85.0 81 53.0 82 45.5 83 38.0 84 29.0 85 24.0 86 21.5 87 20.0 88 15.5 89 10.0 90 8.5 91 12.5 92 9.0 93 4.0 94 5.0 95 5.0 96 5.0 97 3.0 98 2.0 99 2.0 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06140045359482896 2 0.04836001105371681 3 0.044435614552316295 4 0.04414946064075584 5 0.04325011977585155 6 0.005150770408088181 7 3.6791217200629866E-4 8 0.0 9 0.0010219782555730519 10-11 0.0038017591107317524 12-13 0.002800220420270162 14-15 0.008216705174807337 16-17 0.003188572157387922 18-19 0.005314286928979869 20-21 0.010219782555730519 22-23 0.016433410349614673 24-25 0.0038630778060661356 26-27 0.016535608175171978 28-29 0.007623957786574966 30-31 0.007256045614568668 32-33 0.0029228578109389285 34-35 0.01853868555609516 36-37 0.01629033339383445 38-39 0.026694072035568117 40-41 0.0061523090985497715 42-43 0.001594286078693961 44-45 3.270330417833766E-4 46-47 0.0024731873784867853 48-49 0.0037200008502859083 50-51 0.0020235169460346425 52-53 0.0019213191204773376 54-55 0.006213627793884156 56-57 0.0029841765062733113 58-59 0.007828353437689577 60-61 0.01639253121939175 62-63 0.01240681602265685 64-65 0.002779780855158701 66-67 0.004394506498964123 68-69 0.01026066168595344 70-71 0.0026571434644899346 72-73 0.0035564843293942206 74-75 0.0012059343415762012 76-77 2.657143464489935E-4 78-79 0.003249890852722305 80-81 0.002820659985381623 82-83 0.0017373630344741883 84-85 0.012161541241319317 86-87 0.006438463010110226 88-89 0.002309670857595097 90-91 9.402199951272076E-4 92-93 0.002350549987818019 94-95 0.0 96-97 0.0017782421646971103 98-99 0.02456835726397617 100-101 0.014573409924471719 102-103 0.0066632982263362985 104-105 0.014164618622242497 106-107 0.03245802939700013 108-109 0.0013490112973564283 110-111 0.0019008795553658762 112-113 0.016045058612496915 114-115 0.03176308418321045 116-117 0.05091495669264944 118-119 0.3567317298903295 120-121 0.11726178504445196 122-123 0.020051213374343277 124-125 0.3178965561785535 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2446236.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.31009996677518 #Duplication Level Percentage of deduplicated Percentage of total 1 81.62795491484506 50.86246030827412 2 9.11004206764004 11.352952638723561 3 3.433181915400177 6.417657250581289 4 1.7565899010050214 4.378131693290023 5 1.0326321951401711 3.2171707654047275 6 0.6756811637032515 2.526105651360995 7 0.47471248441465946 2.070556765154754 8 0.3438794484853604 1.7141730249313856 9 0.24725342085457164 1.3865746833517945 >10 1.2433864678949837 13.402296726688293 >50 0.04837889474242108 2.0112815199649967 >100 0.006145404793506241 0.511803798713048 >500 0.0 0.0 >1k 1.6172108072815857E-4 0.1488351735610087 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 4.087913022292207E-5 0.0 0.0 0.0 0.0 3 4.087913022292207E-5 0.0 0.0 0.0 0.0 4 4.087913022292207E-5 0.0 0.0 0.0 0.0 5 1.2263739066876623E-4 0.0 0.0 0.0 0.0 6 2.861539115604545E-4 0.0 0.0 0.0 0.0 7 7.358243440125973E-4 0.0 0.0 0.0 0.0 8 8.175826044584415E-4 0.0 0.0 0.0 0.0 9 8.175826044584415E-4 0.0 0.0 0.0 0.0 10-11 9.402199951272077E-4 0.0 0.0 0.0 0.0 12-13 0.0013081321671335063 0.0 0.0 2.0439565111461036E-5 0.0 14-15 0.0018191212949200323 0.0 0.0 6.13186953343831E-5 0.0 16-17 0.0018804399902544154 0.0 0.0 2.657143464489935E-4 0.0 18-19 0.0025753852040440906 0.0 0.0 3.8835173711775973E-4 0.0 20-21 0.0031476930271649994 2.0439565111461036E-5 0.0 4.2923086734068176E-4 0.0 22-23 0.0036791217200629867 4.087913022292207E-5 0.0 6.336265184552921E-4 0.0 24-25 0.005273407798756948 4.087913022292207E-5 0.0 0.0010424178206845129 0.0 26-27 0.005273407798756948 4.087913022292207E-5 0.0 0.002187033466926331 0.0 28-29 0.006479342140333149 4.087913022292207E-5 0.0 0.005273407798756948 0.0 30-31 0.007031210398342596 4.087913022292207E-5 0.0 0.015043519922035321 0.0 32-33 0.00711296865878844 4.087913022292207E-5 0.0 0.03450198590814623 0.0 34-35 0.007603518221463506 4.087913022292207E-5 0.0 0.06371012445242405 0.0 36-37 0.008584617346813636 4.087913022292207E-5 0.0 0.1101079372554406 0.0 38-39 0.00985187038372422 4.087913022292207E-5 0.0 0.17719058995125572 0.0 40-41 0.009933628644170064 4.087913022292207E-5 0.0 0.2525308269521011 0.0 42-43 0.010894288204408731 4.087913022292207E-5 0.0 0.3254183161395712 0.0 44-45 0.012284178631988083 4.087913022292207E-5 0.0 0.41545460045555704 0.0 46-47 0.014082860361796654 4.087913022292207E-5 0.0 0.5259100103178924 2.0439565111461036E-5 48-49 0.014757366010474868 4.087913022292207E-5 0.0 0.6405146518978545 4.087913022292207E-5 50-51 0.014757366010474868 4.087913022292207E-5 0.0 0.7659318234217793 4.087913022292207E-5 52-53 0.01477780557558633 4.087913022292207E-5 0.0 0.8903883353854656 4.087913022292207E-5 54-55 0.014941322096478016 4.087913022292207E-5 0.0 1.0275582568484807 4.087913022292207E-5 56-57 0.016106377307831297 4.087913022292207E-5 0.0 1.1879475242781155 4.087913022292207E-5 58-59 0.016229014698500063 4.087913022292207E-5 0.0 1.366814158568511 4.087913022292207E-5 60-61 0.01639253121939175 4.087913022292207E-5 0.0 1.5628909066827568 4.087913022292207E-5 62-63 0.016699124696063668 4.087913022292207E-5 0.0 1.760541501310585 4.087913022292207E-5 64-65 0.016801322521620973 4.087913022292207E-5 0.0 1.9729699015139994 4.087913022292207E-5 66-67 0.0177824216469711 4.087913022292207E-5 0.0 2.218203803721309 4.087913022292207E-5 68-69 0.01819121294920032 4.087913022292207E-5 0.0 2.4963454057580705 4.087913022292207E-5 70-71 0.018804399902544153 4.087913022292207E-5 0.0 2.805943498501371 4.087913022292207E-5 72-73 0.019233630769884835 4.087913022292207E-5 0.0 3.1247598351099404 4.087913022292207E-5 74-75 0.019438026420999448 4.087913022292207E-5 0.0 3.4503212282052917 4.087913022292207E-5 76-77 0.01949934511633383 4.087913022292207E-5 0.0 3.8150448280542024 4.087913022292207E-5 78-79 0.019642422072114057 4.087913022292207E-5 0.0 4.2112044790445395 4.087913022292207E-5 80-81 0.022238246841269606 4.087913022292207E-5 0.0 4.656296448911716 4.087913022292207E-5 82-83 0.022606159013275905 4.087913022292207E-5 0.0 5.121787104760129 4.087913022292207E-5 84-85 0.023198906401508276 4.087913022292207E-5 0.0 5.589853145812587 4.087913022292207E-5 86-87 0.023812093354852105 8.175826044584414E-5 0.0 6.082324027608129 4.087913022292207E-5 88-89 0.023832532919963567 8.175826044584414E-5 0.0 6.620518216558009 4.087913022292207E-5 90-91 0.025488137693991914 8.175826044584414E-5 0.0 7.2073585704731675 4.087913022292207E-5 92-93 0.02608088508222428 8.175826044584414E-5 0.0 7.8144749729788945 4.087913022292207E-5 94-95 0.02608088508222428 8.175826044584414E-5 0.0 8.45317050358183 4.087913022292207E-5 96-97 0.026142203777558666 8.175826044584414E-5 0.0 9.092131748531212 4.087913022292207E-5 98-99 0.02745033594469217 8.175826044584414E-5 0.0 9.767168825902324 4.087913022292207E-5 100-101 0.028901545067605908 8.175826044584414E-5 0.0 10.50969734727148 4.087913022292207E-5 102-103 0.03018923766962795 8.175826044584414E-5 0.0 11.280064556322447 4.087913022292207E-5 104-105 0.03290769982945227 8.175826044584414E-5 0.0 12.05231220536367 4.087913022292207E-5 106-107 0.03366396373857633 8.175826044584414E-5 0.0 12.825581832660463 4.087913022292207E-5 108-109 0.034031875910582626 8.175826044584414E-5 0.0 13.630471467184687 4.087913022292207E-5 110-111 0.034645062863926455 8.175826044584414E-5 0.0 14.486889245354906 4.087913022292207E-5 112-113 0.03499253547082129 8.175826044584414E-5 0.0 15.374150327278317 4.087913022292207E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2340 0.0 46.78266 1 GTATCAA 3330 0.0 41.80754 1 GTACATG 8150 0.0 35.84321 1 ACCGTAC 70 0.0027979505 33.97927 8 AACGCAG 3975 0.0 33.95929 6 TACATGG 8525 0.0 33.495422 2 CAACGCA 4155 0.0 32.643257 5 ATCAACG 4205 0.0 32.25511 3 TCAACGC 4210 0.0 32.216145 4 TATCAAC 4400 0.0 31.63747 2 ACATGGG 8715 0.0 31.604092 3 CATGGGG 6685 0.0 28.119429 4 ACGCAGA 4910 0.0 27.0075 7 CGCAGAG 5190 0.0 24.74791 8 GAGTACA 6915 0.0 23.402363 1 AGTACAT 6755 0.0 22.985489 2 ATGGGGA 3685 0.0 22.116318 5 CAGAGTA 5440 0.0 19.786018 10-11 AGAGTAC 5100 0.0 19.356049 10-11 GCAGAGT 6610 0.0 19.252676 9 >>END_MODULE