FastQCFastQC Report
Thu 26 May 2016
SRR1364197_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364197_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2446236
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43660.17847828255327777No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39630.16200399307344016No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24840.10154375947373842No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35100.065.3118741
GTATCAA61550.054.561911
ATCAACG80300.041.5822683
TATCAAC82600.040.6422422
CAACGCA82500.040.3847435
TCAACGC82550.040.3767854
AACGCAG83600.039.9940646
GTACATG80200.036.6025281
ACGCAGA97100.034.0639047
TACATGG85500.033.9032552
CGCAGAG100400.032.8257688
ACATGGG85500.032.4399833
CATGGGG63200.028.5355594
GCAGAGT118300.027.7080359
GAGTACT71300.025.8237212-13
CAGAGTA107550.025.30576910-11
AGAGTAC104000.024.68233910-11
CTATGCG2201.5428486E-724.3365339
GTACTTT77300.023.62689414-15
AGTACTT74850.022.7311812-13
Produced by FastQC (version 0.11.5)