FastQCFastQC Report
Thu 26 May 2016
SRR1364196_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364196_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1551496
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23410.150886628131816No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16150.058.2087941
GTATCAA26950.040.8429371
GTACATG68950.038.2272871
TACATGG71200.036.095392
ACATGGG70550.034.9933133
TCAACGC29850.034.8738374
ATCAACG30350.034.4975243
CAACGCA30800.033.60835
AACGCAG32600.031.7403476
TATCAAC36950.028.8195112
CATGGGG28900.027.1695274
CATGGGA45450.026.6994464
ACGCAGA38600.026.6525527
CGCAGAG39300.026.0265088
ATGGGTA7750.024.5638395
GAGTACA48850.023.0197831
TCTTACG1602.61464E-422.3089582
GCAGAGT45550.021.5421759
GTCTTAC4151.8189894E-1221.5053841
CAGAGTA45250.021.02787610-11
Produced by FastQC (version 0.11.5)