##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364196_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1551496 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.199778149605283 31.0 31.0 34.0 26.0 34.0 2 30.17709359224903 31.0 31.0 34.0 26.0 34.0 3 30.273090617056056 31.0 31.0 34.0 26.0 34.0 4 33.60954781707462 37.0 35.0 37.0 28.0 37.0 5 33.74650401934649 37.0 35.0 37.0 28.0 37.0 6 33.84533830573846 37.0 35.0 37.0 29.0 37.0 7 33.69858124029969 37.0 35.0 37.0 28.0 37.0 8 33.64990370584262 37.0 35.0 37.0 28.0 37.0 9 35.0412002351279 39.0 35.0 39.0 27.0 39.0 10-11 34.99286752914606 39.0 35.0 39.0 27.0 39.0 12-13 34.95492382835663 39.0 35.0 39.0 27.0 39.0 14-15 36.14172095835245 40.0 36.0 41.0 27.0 41.0 16-17 36.16995274238542 40.0 36.0 41.0 27.0 41.0 18-19 36.05527729365722 40.0 36.0 41.0 27.0 41.0 20-21 36.038520241109225 40.0 36.0 41.0 27.0 41.0 22-23 36.059990164331715 40.0 36.0 41.0 27.0 41.0 24-25 35.926545089384696 40.0 36.0 41.0 26.5 41.0 26-27 35.93846713107865 40.0 36.0 41.0 27.0 41.0 28-29 36.02982250679344 40.0 36.0 41.0 27.0 41.0 30-31 35.928917315932495 40.0 36.0 41.0 27.0 41.0 32-33 35.75342959311529 40.0 35.5 41.0 26.5 41.0 34-35 35.71245752486632 40.0 35.0 41.0 26.0 41.0 36-37 35.72895257222706 40.0 35.0 41.0 26.5 41.0 38-39 35.5785416140293 40.0 35.0 41.0 25.5 41.0 40-41 35.58730960311854 40.0 35.0 41.0 26.0 41.0 42-43 35.51133776690368 40.0 35.0 41.0 26.0 41.0 44-45 35.50604352186535 40.0 35.0 41.0 26.0 41.0 46-47 35.444824865806936 40.0 35.0 41.0 26.0 41.0 48-49 35.36555846744046 40.0 35.0 41.0 26.0 41.0 50-51 35.264441545450325 39.0 35.0 41.0 26.0 41.0 52-53 35.138729974166864 39.0 34.5 41.0 26.0 41.0 54-55 34.96298830290249 39.0 34.0 41.0 26.0 41.0 56-57 34.790304647901124 39.0 34.0 41.0 25.0 41.0 58-59 34.58594446908016 38.5 34.0 40.0 24.5 41.0 60-61 34.359542338491366 38.0 34.0 40.0 24.0 41.0 62-63 34.087271897574986 38.0 33.0 40.0 23.0 41.0 64-65 33.800477410189906 37.0 33.0 40.0 23.0 41.0 66-67 33.508793770657476 37.0 33.0 40.0 22.0 41.0 68-69 33.29919767759633 36.0 33.0 39.0 22.0 41.0 70-71 33.1506816646643 36.0 33.0 39.0 22.0 41.0 72-73 32.84207725962555 36.0 33.0 39.0 22.0 40.5 74-75 32.498796000763136 35.0 32.5 38.5 21.5 40.0 76-77 32.165401328782025 35.0 32.0 37.0 20.5 39.5 78-79 31.810197705956057 35.0 32.0 37.0 20.0 39.0 80-81 31.496002245574594 35.0 32.0 36.0 19.0 39.0 82-83 31.20041366526243 35.0 32.0 36.0 18.0 37.0 84-85 30.924746824999872 35.0 32.0 35.5 16.5 37.0 86-87 30.709796544754226 35.0 31.5 35.0 13.5 37.0 88-89 30.52004484703796 35.0 31.0 35.0 10.5 36.0 90-91 30.35050106477877 35.0 31.0 35.0 7.5 36.0 92-93 30.20990901684568 35.0 31.0 35.0 7.0 36.0 94-95 30.073267027436742 35.0 31.0 35.0 6.0 35.5 96-97 29.96915912126103 35.0 31.0 35.0 2.0 35.0 98-99 29.887826330200014 35.0 31.0 35.0 2.0 35.0 100-101 29.79661307538015 35.0 31.0 35.0 2.0 35.0 102-103 28.82016486023812 33.5 29.0 34.5 2.0 35.0 104-105 29.49820141334557 34.0 30.5 35.0 2.0 35.0 106-107 29.60811790684604 34.0 31.0 35.0 2.0 35.0 108-109 29.585527774483467 35.0 31.0 35.0 2.0 35.0 110-111 29.51885148269799 34.0 31.0 35.0 2.0 35.0 112-113 29.661739379282963 35.0 31.0 35.0 2.0 35.0 114-115 29.65494077973775 35.0 31.0 35.0 2.0 35.0 116-117 29.569787482532988 35.0 31.0 35.0 2.0 35.0 118-119 29.342957055641783 35.0 31.0 35.0 2.0 35.0 120-121 29.283112234901026 35.0 31.0 35.0 2.0 35.0 122-123 29.154354571329865 35.0 31.0 35.0 2.0 35.0 124-125 28.28937780052285 34.5 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7611767911702962 1101 2 0.8029850547620931 1101 3 0.8586687340893242 1101 4 0.7509634588143328 1101 5 0.5478129860066829 1101 6 0.6735679887012296 1101 7 0.7698873371362609 1101 8 0.7846816291620655 1101 9 0.8536934476942761 1101 10-11 0.8597180755107416 1101 12-13 0.8735353644394976 1101 14-15 0.9721552724205651 1101 16-17 0.9124457768308005 1101 18-19 0.9849888027683136 1101 20-21 1.0116862068762913 1101 22-23 1.054218191383633 1101 24-25 1.3208278634199857 1101 26-27 1.3595358656401473 1101 28-29 1.2024555784367763 1101 30-31 1.1953122733403134 1101 32-33 1.3601365363794216 1101 34-35 1.2563585958799734 1101 36-37 1.1324896372331565 1101 38-39 1.2698939019501694 1101 40-41 1.2652229957595367 1101 42-43 1.2174492168346873 1101 44-45 1.1022877306460614 1101 46-47 1.0372555009479925 1101 48-49 1.028647372943972 1101 50-51 0.9310321376201358 1101 52-53 0.8194590385789766 1101 54-55 0.8299609376339347 1101 56-57 0.8626324168352966 1101 58-59 0.8792903673141979 1101 60-61 0.8034854563707015 1101 62-63 0.7772377413931366 1101 64-65 0.7328210003696682 1101 66-67 0.7020025680206672 1101 68-69 0.7972370637449657 1101 70-71 0.8686761028050043 1101 72-73 0.7421769199120689 1101 74-75 0.8193347701354909 1101 76-77 0.8767491981927336 1101 78-79 0.7671464783290887 1101 80-81 0.8248267886891973 1101 82-83 0.8509223125875209 1101 84-85 0.86392669081701 1101 86-87 0.8183230613072574 1101 88-89 0.8429664692914862 1101 90-91 0.844989075257363 1101 92-93 0.7511688375708552 1101 94-95 0.8850577931976069 1101 96-97 0.8288365354661806 1101 98-99 0.8627533575989972 1101 100-101 0.8786743917621145 1101 102-103 0.6531774881424042 1101 104-105 0.8336589856215717 1101 106-107 0.7929304121815974 1101 108-109 0.7407841716076646 1101 110-111 0.6473607761106521 1101 112-113 0.8094779979698963 1101 114-115 0.7742330876723784 1101 116-117 0.8550713857946057 1101 118-119 0.8257696895412607 1101 120-121 0.8006684252569336 1101 122-123 0.8119211949244018 1101 124-125 0.7943448835452074 1102 1 0.12113703960534394 1102 2 0.06722367344006841 1102 3 -0.008080945437129117 1102 4 0.055532707096659806 1102 5 -0.03443631722969798 1102 6 0.185651375642216 1102 7 0.19914347761734774 1102 8 0.1979598670764915 1102 9 0.19391309670361778 1102 10-11 0.192909021467905 1102 12-13 0.25438608381518435 1102 14-15 0.1344938046262385 1102 16-17 0.25137333686237895 1102 18-19 0.3622106830116252 1102 20-21 0.4578368644103108 1102 22-23 0.3443458934899368 1102 24-25 0.5220138856964454 1102 26-27 0.572943006608341 1102 28-29 0.390726420023654 1102 30-31 0.34088800531167607 1102 32-33 0.6833817411022309 1102 34-35 0.5268384712424279 1102 36-37 0.4359661048816932 1102 38-39 0.5885556264495619 1102 40-41 0.5752289270942939 1102 42-43 0.4325129108879153 1102 44-45 0.39985152002066826 1102 46-47 0.36775185926341436 1102 48-49 0.2901736650278579 1102 50-51 0.2419727282375348 1102 52-53 0.25373560842018605 1102 54-55 0.32560785989511487 1102 56-57 0.3412150786201451 1102 58-59 0.2004737756438857 1102 60-61 0.14076094902932113 1102 62-63 0.2566907332713484 1102 64-65 0.297207382481794 1102 66-67 0.2754295970051004 1102 68-69 0.28866904781008174 1102 70-71 0.30295002199816423 1102 72-73 0.21157661541377593 1102 74-75 0.2629020652979932 1102 76-77 0.24856681484826737 1102 78-79 0.13973961343608465 1102 80-81 0.17909803276678105 1102 82-83 0.1282082365657118 1102 84-85 0.16056846246123158 1102 86-87 0.1593379047324568 1102 88-89 0.13836722790695788 1102 90-91 0.10511297062527447 1102 92-93 0.21153267775635243 1102 94-95 0.22319535816141922 1102 96-97 0.2990808701647367 1102 98-99 0.23315522235029107 1102 100-101 0.2236562358424301 1102 102-103 0.29592069682527367 1102 104-105 0.34797085393900673 1102 106-107 0.30670348530829017 1102 108-109 0.31483736522058337 1102 110-111 0.34950001220611426 1102 112-113 0.35449201683965725 1102 114-115 0.3322762650979918 1102 116-117 0.34535115354074364 1102 118-119 0.23137674809468933 1102 120-121 0.34806486698620986 1102 122-123 0.49927929604907106 1102 124-125 0.3651638787416225 1103 1 0.3529011161943423 1103 2 0.09067357348683913 1103 3 0.41355013780064453 1103 4 0.47787023117061267 1103 5 0.3449905910898252 1103 6 0.43841686093864496 1103 7 0.5485118650458816 1103 8 0.501825036661053 1103 9 0.5835800179990684 1103 10-11 0.5363329895305782 1103 12-13 0.5828459891234914 1103 14-15 0.6687002304550234 1103 16-17 0.5789507874458053 1103 18-19 0.7234783447779307 1103 20-21 0.8640750311784586 1103 22-23 0.8205762663511607 1103 24-25 1.0037430278518613 1103 26-27 0.990509371596545 1103 28-29 0.783112760868832 1103 30-31 0.956061316875342 1103 32-33 1.0840977826959701 1103 34-35 1.0089301761680076 1103 36-37 0.8893900073491281 1103 38-39 1.149501951058923 1103 40-41 1.1333053606533738 1103 42-43 1.0041693476790456 1103 44-45 0.9518021534420811 1103 46-47 0.9320305213511304 1103 48-49 0.8438155623363244 1103 50-51 0.8060262793947714 1103 52-53 0.8612303016660405 1103 54-55 0.772737998660844 1103 56-57 0.7952362953004979 1103 58-59 0.7908832946658038 1103 60-61 0.7565229281608978 1103 62-63 0.7197756216590676 1103 64-65 0.7584139611673564 1103 66-67 0.6910035563316725 1103 68-69 0.6641914492795777 1103 70-71 0.640219765320019 1103 72-73 0.5956792715637391 1103 74-75 0.7019073393539017 1103 76-77 0.6416489621501213 1103 78-79 0.6540741606788743 1103 80-81 0.660804117202165 1103 82-83 0.579899232108616 1103 84-85 0.5448809239861419 1103 86-87 0.5745427417650681 1103 88-89 0.5926956489520556 1103 90-91 0.5826441169338601 1103 92-93 0.4902270661632464 1103 94-95 0.5685261599161144 1103 96-97 0.5818921548514453 1103 98-99 0.5737588933380913 1103 100-101 0.6579099299972775 1103 102-103 0.5312757151492029 1103 104-105 0.5516347256522138 1103 106-107 0.5752201762153746 1103 108-109 0.549035422687183 1103 110-111 0.7162192345725842 1103 112-113 0.6746930841338106 1103 114-115 0.6809187318923904 1103 116-117 0.6440085581598005 1103 118-119 0.48008556546621506 1103 120-121 0.39756502133379 1103 122-123 0.4522284281346245 1103 124-125 0.4383073397014208 1104 1 0.45844923385728364 1104 2 0.5539733213452287 1104 3 0.5086700745559583 1104 4 0.5439228442879909 1104 5 0.5112224800178211 1104 6 0.5792942807346861 1104 7 0.53032901786775 1104 8 0.6926691775372902 1104 9 0.7033186915231369 1104 10-11 0.7404061642452007 1104 12-13 0.6943059049740938 1104 14-15 0.7557722101940882 1104 16-17 0.6673607035617053 1104 18-19 0.8333653968905352 1104 20-21 0.9206344565997782 1104 22-23 0.8909409903424645 1104 24-25 1.1622565065512802 1104 26-27 1.0542701901931224 1104 28-29 0.8963212201403294 1104 30-31 0.8756675232022886 1104 32-33 0.9682184616684637 1104 34-35 0.9532488543965272 1104 36-37 0.9172293417943109 1104 38-39 0.9823657244344872 1104 40-41 0.9107248029705985 1104 42-43 0.8283458196890834 1104 44-45 0.7187497896955293 1104 46-47 0.7237964499862741 1104 48-49 0.6650287661383132 1104 50-51 0.6814295910157426 1104 52-53 0.5247488827401554 1104 54-55 0.517226343375043 1104 56-57 0.5307785353579462 1104 58-59 0.6221058189234583 1104 60-61 0.6580647826662798 1104 62-63 0.6261666480823465 1104 64-65 0.5813609701617963 1104 66-67 0.5664130580085924 1104 68-69 0.4508143257219359 1104 70-71 0.5067055644473939 1104 72-73 0.5006654500531198 1104 74-75 0.4302240155414623 1104 76-77 0.36230058049603286 1104 78-79 0.4986854811710799 1104 80-81 0.49169119938075667 1104 82-83 0.3609643226101795 1104 84-85 0.4615282835735677 1104 86-87 0.44330353383283594 1104 88-89 0.42206115642758135 1104 90-91 0.48182844441965855 1104 92-93 0.5136343205550915 1104 94-95 0.47940114495681385 1104 96-97 0.5164320185169373 1104 98-99 0.5080543971762239 1104 100-101 0.424774147877816 1104 102-103 0.3735321615029399 1104 104-105 0.5285738196284662 1104 106-107 0.5815045568177482 1104 108-109 0.5118726849374475 1104 110-111 0.6118139191420688 1104 112-113 0.5167169612429241 1104 114-115 0.5461986491696607 1104 116-117 0.49391208171649836 1104 118-119 0.45381791570076047 1104 120-121 0.5876504382104777 1104 122-123 0.631667538322926 1104 124-125 0.5302199431858945 1105 1 0.6183945012572991 1105 2 0.4502894561749997 1105 3 0.4360442850905599 1105 4 0.6869702845828414 1105 5 0.537879866500127 1105 6 0.4521622048549503 1105 7 0.5379018911224733 1105 8 0.39884454077247256 1105 9 0.6391040650647852 1105 10-11 0.6902550387572646 1105 12-13 0.7074713603035363 1105 14-15 0.6309239474685029 1105 16-17 0.7173318974293466 1105 18-19 0.9216703727349298 1105 20-21 0.8667298076485537 1105 22-23 0.77602766578471 1105 24-25 1.0674222039395005 1105 26-27 1.0542075887859568 1105 28-29 0.9977189957416002 1105 30-31 0.934566341686093 1105 32-33 1.0368094007407933 1105 34-35 1.0606149153661235 1105 36-37 0.953700162804175 1105 38-39 1.092945798558354 1105 40-41 1.0556854750593132 1105 42-43 1.0026181779239351 1105 44-45 0.8399076046180696 1105 46-47 0.8808309416543096 1105 48-49 0.899566645396142 1105 50-51 0.7846578886729105 1105 52-53 0.7903084150659083 1105 54-55 0.7110257878160056 1105 56-57 0.6976666782151142 1105 58-59 0.8025404320382208 1105 60-61 0.7743726009939635 1105 62-63 0.7822546254441676 1105 64-65 0.7932858932426967 1105 66-67 0.7282795246542761 1105 68-69 0.7214777697440411 1105 70-71 0.8026675650138415 1105 72-73 0.7098354369656263 1105 74-75 0.6390354671220351 1105 76-77 0.6246206797021898 1105 78-79 0.6784779556093099 1105 80-81 0.5897954770107852 1105 82-83 0.5572962785228555 1105 84-85 0.6498203217938148 1105 86-87 0.5909477195753823 1105 88-89 0.5999724585804849 1105 90-91 0.5421345615066748 1105 92-93 0.5759311623899812 1105 94-95 0.5349474683856528 1105 96-97 0.5649645182271712 1105 98-99 0.6271308101611233 1105 100-101 0.6395524620674458 1105 102-103 0.5879839050185218 1105 104-105 0.5814629030243488 1105 106-107 0.5733737722343122 1105 108-109 0.5316685777919119 1105 110-111 0.5650588251422626 1105 112-113 0.6648943971537875 1105 114-115 0.7496732359387224 1105 116-117 0.6770225255851408 1105 118-119 0.5543158434993316 1105 120-121 0.4560976596280497 1105 122-123 0.5419318357729423 1105 124-125 0.5570850525192519 1106 1 0.3977784106589901 1106 2 0.374821030127066 1106 3 0.4442768256579974 1106 4 0.3179141398024754 1106 5 0.33539560049199935 1106 6 0.2623758922760473 1106 7 0.21637723736244396 1106 8 0.20103983577905638 1106 9 0.25725984770993904 1106 10-11 0.386142177889063 1106 12-13 0.45032448821190485 1106 14-15 0.4910684329692714 1106 16-17 0.5680021700904447 1106 18-19 0.5320404325379187 1106 20-21 0.46684213406783215 1106 22-23 0.42346249526786295 1106 24-25 0.6789970774686154 1106 26-27 0.5462066120344815 1106 28-29 0.6326639382883386 1106 30-31 0.5615402550500832 1106 32-33 0.8078538797913168 1106 34-35 0.7809469187202467 1106 36-37 0.659637613851423 1106 38-39 0.6639053186600847 1106 40-41 0.5924163063116694 1106 42-43 0.5043189233880341 1106 44-45 0.48679361034957935 1106 46-47 0.4210911629898817 1106 48-49 0.31194715459695743 1106 50-51 0.370451314951211 1106 52-53 0.40137500924410574 1106 54-55 0.3486969727261169 1106 56-57 0.3385215200458802 1106 58-59 0.33183210292826004 1106 60-61 0.26447506775216567 1106 62-63 0.39203863436393505 1106 64-65 0.3503040368620205 1106 66-67 0.3365886388512678 1106 68-69 0.36650085240854224 1106 70-71 0.42269917121713974 1106 72-73 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64-65 -2.598014545712644 2216 66-67 -2.5061943939723434 2216 68-69 -2.8323970250037718 2216 70-71 -2.8568676044952497 2216 72-73 -2.789059818906395 2216 74-75 -3.0944836621320846 2216 76-77 -3.0299227084975513 2216 78-79 -3.0981490792973645 2216 80-81 -3.101592474266564 2216 82-83 -3.0504483210841826 2216 84-85 -2.989446350413349 2216 86-87 -2.857771625517227 2216 88-89 -2.7644009929409457 2216 90-91 -2.956700987767359 2216 92-93 -2.9916931843432657 2216 94-95 -2.9314815935514247 2216 96-97 -2.936268070287859 2216 98-99 -2.8638061756330124 2216 100-101 -3.002795122215641 2216 102-103 -2.9812466696463 2216 104-105 -3.0137034779281784 2216 106-107 -3.0980607329829617 2216 108-109 -3.055914979183065 2216 110-111 -2.9887492943700416 2216 112-113 -3.1055415625299787 2216 114-115 -3.1459327025793122 2216 116-117 -3.086652823315614 2216 118-119 -3.1518843339686384 2216 120-121 -2.9321343419728443 2216 122-123 -2.8883156300088935 2216 124-125 -2.594021678960903 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 44079.0 3 8154.0 4 5631.0 5 4741.0 6 4594.0 7 4785.0 8 4910.0 9 5023.0 10 5860.0 11 6542.0 12 6862.0 13 6900.0 14 6933.0 15 6869.0 16 6679.0 17 6190.0 18 5661.0 19 5212.0 20 4741.0 21 5114.0 22 5806.0 23 7619.0 24 11923.0 25 17581.0 26 20419.0 27 19151.0 28 21609.0 29 25613.0 30 30292.0 31 35881.0 32 44949.0 33 59222.0 34 82496.0 35 125089.0 36 241766.0 37 456264.0 38 189856.0 39 480.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.45460900880449 19.960762866864616 12.705698284246072 23.87892984008482 2 15.35282980907623 21.401221066098522 39.81239069988187 23.43355842494338 3 18.24801656899967 26.599189604655 29.669832246616046 25.482961579729285 4 11.8912502264868 17.592849916465862 37.999230106076425 32.51666975097091 5 12.638035781298965 39.76049510402575 34.8168055783027 12.784663536372582 6 30.641888538386077 38.49010778498693 17.83512158522955 13.032882091397441 7 27.359336377728432 33.74753864845616 21.776298191732437 17.116826782082974 8 25.06658089998298 37.223943858057 20.166342678292434 17.543132563667584 9 25.865013877090103 17.28603306008851 20.45256200861372 36.39639105420767 10-11 23.751507067141794 28.40585894206062 28.41655867563081 19.426075315166777 12-13 23.99846337644022 26.258440874960158 28.098296210617978 21.64479953798164 14-15 21.786961560724567 27.54795401777254 25.312366651153617 25.352717770349276 16-17 21.85921542706188 30.536486544409424 26.245870410076215 21.35842761845248 18-19 21.588801402080755 29.48813932629607 27.88063234151418 21.042426930108995 20-21 22.38051834953753 28.65915878265791 27.690516692912677 21.269806174891883 22-23 22.376298385885473 28.62334512809967 27.196756428512952 21.803600057501903 24-25 22.071934303170078 28.796571162273242 27.583979807010245 21.547514727546435 26-27 21.83542233208263 28.919012042779375 27.531625067727138 21.713940557410854 28-29 21.77444098518103 28.60879598295722 27.774318514364538 21.84244451749721 30-31 21.825455656483097 28.538734346275724 27.770744649307588 21.865065347933594 32-33 21.591015662389285 28.742300375357203 27.790613394970077 21.87607056728343 34-35 22.19185553090478 28.6437907601382 27.555972717037413 21.60838099191961 36-37 22.164538247247336 28.352860900611336 27.348811633833858 22.13378921830747 38-39 21.758639919798075 28.858895547310432 27.579339623553835 21.803124909337658 40-41 21.956591450749233 28.411892956303003 27.865201129930757 21.766314463017007 42-43 21.75687517100712 28.465091666261138 27.655279297930573 22.12275386480117 44-45 21.901097845752446 28.712214621657978 27.469991807890853 21.916695724698727 46-47 21.947148611617354 28.65091573214581 27.268898043685848 22.133037612550986 48-49 21.824510811204515 28.794137855909906 27.2976629544309 22.08368837845468 50-51 21.632760135381197 28.611846434770616 27.395774719604816 22.359618710243375 52-53 22.039487889817547 28.555047719475102 27.464786462841843 21.940677927865504 54-55 21.86766593163496 28.33447315888094 27.608100259957574 22.189760649526526 56-57 21.82324216735763 28.859600272260376 27.389368438421712 21.927789121960277 58-59 21.954833906477116 28.365138247436967 27.383821552999414 22.296206293086502 60-61 21.97789553075867 28.603047825330215 27.526607231494193 21.892449412416923 62-63 21.919543819716996 28.454992904413768 27.677878756527118 21.94758451934212 64-65 21.66497685070299 28.259352051724484 27.576861918294554 22.498809179277977 66-67 21.630899046780495 28.745544826003684 27.17486132923474 22.448694797981084 68-69 21.693848491274977 28.359073396405567 27.571304347265563 22.375773765053893 70-71 21.496162619589814 28.395035866470636 27.834656504983407 22.274145008956143 72-73 21.829823206125663 28.268198663241463 27.599110374590826 22.30286775604205 74-75 21.467840473252416 28.436911548450784 27.575441845235815 22.51980613306099 76-77 21.673934957924264 28.33989047933339 27.797507940763964 22.188666621978385 78-79 21.604373483868702 28.387456689444424 27.580883701308558 22.427286125378316 80-81 21.551868329839298 28.44792218904543 27.61183471617726 22.388374764938018 82-83 21.76571536303532 28.3860602945276 27.57402265919202 22.274201683245057 84-85 21.47981121877215 28.338714236493512 27.487052151410463 22.694422393323876 86-87 21.609082711148154 28.18239420595893 27.861358900132593 22.34716418276033 88-89 21.762781323176895 28.02628873521315 27.716162099901837 22.494767841708114 90-91 21.594095194143407 28.267520989608645 27.90135496254549 22.23702885370246 92-93 21.787587067490538 28.088351930398215 27.80668076311616 22.317380238995085 94-95 21.55864404419992 27.75221463671192 27.891821828738202 22.79731949034996 96-97 21.931180372754717 27.74983306219368 27.702104318726168 22.616882246325435 98-99 21.35305374945887 27.777672121435025 27.773804024819 23.09547010428711 100-101 21.29149143548183 27.877680682582945 28.019015215782538 22.811812666152683 102-103 21.78807932079259 27.891386889167276 27.604290714849494 22.716243075190643 104-105 21.53666154188302 27.817196711113688 27.851909868270496 22.79423187873279 106-107 21.25099048809061 27.92131930112321 28.00864988655728 22.8190403242289 108-109 21.422418307709908 28.24649406775155 27.354505624477316 22.97658200006123 110-111 21.17477920882572 28.089078329786147 27.72742038820058 23.008722073187553 112-113 21.327121148878085 27.938198326835895 27.981776016477006 22.752904507809014 114-115 21.05789298247537 28.30194640032959 27.785833424133973 22.85432719306107 116-117 21.049570221175124 28.210540841966044 27.733897279330282 23.005991657528554 118-119 21.123842099237457 28.023187445868686 27.634767973537024 23.218202481356837 120-121 20.90619977938014 28.075893489455705 27.856080225872404 23.161826505291746 122-123 21.11778899616625 28.095831482643053 27.730055575503975 23.056323945686728 124-125 21.015592418134148 27.88689273463793 27.86102930003847 23.236485547189453 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1659.0 1 1364.0 2 937.5 3 699.5 4 522.0 5 421.0 6 392.5 7 387.0 8 391.5 9 390.5 10 356.0 11 325.0 12 346.0 13 387.5 14 403.5 15 441.5 16 492.0 17 566.5 18 695.5 19 892.0 20 1222.5 21 1723.0 22 2265.5 23 3040.0 24 4062.0 25 5557.5 26 8154.0 27 11056.5 28 12977.0 29 15411.5 30 19829.5 31 24484.5 32 29629.0 33 35357.0 34 41441.0 35 46225.0 36 50558.0 37 55236.5 38 56740.5 39 56496.5 40 55052.5 41 54592.0 42 54966.0 43 56152.0 44 57692.0 45 57437.5 46 55295.0 47 53032.0 48 54090.0 49 54807.5 50 53329.5 51 50576.0 52 47344.5 53 45852.0 54 44627.5 55 41249.5 56 37157.0 57 33480.5 58 29892.0 59 26096.5 60 21623.5 61 17030.5 62 13567.5 63 10525.5 64 7993.0 65 6414.0 66 4926.5 67 3891.5 68 2885.0 69 1817.0 70 1274.0 71 940.0 72 758.0 73 642.5 74 511.0 75 416.0 76 294.5 77 178.0 78 108.0 79 64.5 80 41.5 81 32.5 82 24.5 83 18.5 84 17.5 85 12.0 86 10.0 87 9.0 88 7.5 89 6.5 90 5.5 91 5.0 92 3.5 93 2.5 94 2.0 95 1.5 96 1.0 97 0.5 98 1.5 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05955542263724818 2 0.04640682283421936 3 0.04176614055079743 4 0.04079933174175119 5 0.04060596997994194 6 0.004769590124628101 7 7.089931266339069E-4 8 0.0 9 0.001160170570855484 10-11 0.0035771925934710755 12-13 0.002739291625631004 14-15 0.007347746948751398 16-17 0.0026103837844248393 18-19 0.005639718052769713 20-21 0.009603634169859284 22-23 0.015662302706549034 24-25 0.004286185720104982 26-27 0.015114444381422834 28-29 0.006832115583926739 30-31 0.006961023425132903 32-33 0.0026103837844248393 34-35 0.017499239443736884 36-37 0.014663266937201257 38-39 0.026490561367866882 40-41 0.004737363164326559 42-43 0.0016435749753786024 44-45 1.9336176180924733E-4 46-47 0.0020947524196001798 48-49 0.003222696030154122 50-51 0.001611348015077061 52-53 0.0021269793799017205 54-55 0.005736398933674338 56-57 0.0025781568241232977 58-59 0.007154385186942151 60-61 0.014566586056296633 62-63 0.011569478748253299 64-65 0.00290042642713871 66-67 0.004479547481914229 68-69 0.009861449852271614 70-71 0.003029334268344875 72-73 0.0036416465140741584 74-75 0.0012568514517601076 76-77 5.156313648246595E-4 78-79 0.0035771925934710755 80-81 0.0028037455462340865 82-83 0.0018369367371878496 84-85 0.011376116986444052 86-87 0.007025477345735987 88-89 0.0021269793799017205 90-91 9.023548884431543E-4 92-93 0.0026748377050279217 94-95 0.0 96-97 0.0012890784120616488 98-99 0.022268829568364984 100-101 0.013664231167853478 102-103 0.006413165100006703 104-105 0.013599777247250396 106-107 0.03177578285731964 108-109 0.0011923975311570253 110-111 0.0019336176180924734 112-113 0.015694529666850577 114-115 0.030873427968876488 116-117 0.04875939093623187 118-119 0.35365866234911336 120-121 0.11418012034836054 122-123 0.017595920324641506 124-125 0.3165332040817379 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1551496.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.90369750253482 #Duplication Level Percentage of deduplicated Percentage of total 1 87.247176913312 48.77439786111933 2 7.129563794508753 7.971379553864834 3 2.0207766789758606 3.389066645449302 4 0.8479595691825645 1.896163009998473 5 0.4823621066824686 1.3482912649331076 6 0.3175596126520785 1.0651653914834354 7 0.20947984653693624 0.819748858157481 8 0.15759533138360912 0.7048129386784813 9 0.1406200116783176 0.7075060736100823 >10 1.1402004133169128 13.342588237174372 >50 0.1933805914006184 7.552370520072839 >100 0.11046601010827699 11.19850363441278 >500 0.002599200237839611 0.889847231144956 >1k 2.599200237839611E-4 0.34015877990044774 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2341 0.150886628131816 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 6.445392060308244E-5 0.0 8 0.0 0.0 0.0 6.445392060308244E-5 0.0 9 0.0 0.0 0.0 6.445392060308244E-5 6.445392060308244E-5 10-11 0.0 0.0 0.0 6.445392060308244E-5 6.445392060308244E-5 12-13 0.0 0.0 0.0 6.445392060308244E-5 6.445392060308244E-5 14-15 0.0 0.0 0.0 6.445392060308244E-5 6.445392060308244E-5 16-17 0.0 0.0 0.0 6.445392060308244E-5 6.445392060308244E-5 18-19 0.0 0.0 0.0 6.445392060308244E-5 6.445392060308244E-5 20-21 0.0 0.0 0.0 1.9336176180924733E-4 6.445392060308244E-5 22-23 0.0 0.0 0.0 3.5449656331695346E-4 6.445392060308244E-5 24-25 0.0 0.0 0.0 6.123122457292832E-4 6.445392060308244E-5 26-27 3.222696030154122E-5 0.0 0.0 0.0013857592929662726 6.445392060308244E-5 28-29 6.445392060308244E-5 0.0 0.0 0.0032871499507572044 6.445392060308244E-5 30-31 6.445392060308244E-5 0.0 0.0 0.00973254201106545 9.668088090462366E-5 32-33 6.445392060308244E-5 0.0 0.0 0.02288114181409427 1.2890784120616488E-4 34-35 6.445392060308244E-5 0.0 0.0 0.04537556010457004 1.2890784120616488E-4 36-37 6.445392060308244E-5 0.0 0.0 0.08188870612621624 1.2890784120616488E-4 38-39 6.445392060308244E-5 0.0 0.0 0.133677431330793 1.2890784120616488E-4 40-41 6.445392060308244E-5 0.0 0.0 0.18791540551828687 1.2890784120616488E-4 42-43 6.445392060308244E-5 0.0 0.0 0.24099320913492528 1.2890784120616488E-4 44-45 1.2890784120616488E-4 0.0 0.0 0.31137689043349126 1.611348015077061E-4 46-47 1.611348015077061E-4 0.0 0.0 0.3973261935577017 1.9336176180924733E-4 48-49 1.9336176180924733E-4 0.0 0.0 0.4838233550070384 1.9336176180924733E-4 50-51 1.9336176180924733E-4 0.0 0.0 0.5790217957377911 1.9336176180924733E-4 52-53 1.9336176180924733E-4 0.0 0.0 0.6680648870509496 1.9336176180924733E-4 54-55 1.9336176180924733E-4 0.0 0.0 0.7703210320877398 1.9336176180924733E-4 56-57 1.9336176180924733E-4 0.0 0.0 0.8958772694225445 1.9336176180924733E-4 58-59 1.9336176180924733E-4 0.0 0.0 1.040318505494052 1.9336176180924733E-4 60-61 1.9336176180924733E-4 0.0 0.0 1.1994874624233645 1.9336176180924733E-4 62-63 1.9336176180924733E-4 0.0 0.0 1.348633834698897 1.9336176180924733E-4 64-65 1.9336176180924733E-4 0.0 0.0 1.5016796691709162 1.9336176180924733E-4 66-67 1.9336176180924733E-4 0.0 0.0 1.6908841531012648 1.9336176180924733E-4 68-69 1.9336176180924733E-4 0.0 0.0 1.9116710581271237 1.9336176180924733E-4 70-71 1.9336176180924733E-4 0.0 0.0 2.1569182260218525 1.9336176180924733E-4 72-73 1.9336176180924733E-4 0.0 0.0 2.403454472328643 2.5781568241232977E-4 74-75 1.9336176180924733E-4 0.0 0.0 2.6641705811681113 2.5781568241232977E-4 76-77 1.9336176180924733E-4 0.0 0.0 2.942579291213126 2.5781568241232977E-4 78-79 1.9336176180924733E-4 0.0 0.0 3.2584357291285313 2.5781568241232977E-4 80-81 2.5781568241232977E-4 0.0 0.0 3.6081627023208567 2.9004264271387096E-4 82-83 2.5781568241232977E-4 0.0 0.0 3.9830911584689876 3.222696030154122E-4 84-85 2.5781568241232977E-4 0.0 0.0 4.347706987320625 3.222696030154122E-4 86-87 2.5781568241232977E-4 0.0 0.0 4.725342508134085 3.222696030154122E-4 88-89 2.5781568241232977E-4 0.0 0.0 5.147096737600355 3.222696030154122E-4 90-91 2.5781568241232977E-4 0.0 0.0 5.618351578089792 3.222696030154122E-4 92-93 2.5781568241232977E-4 0.0 0.0 6.124282627863687 3.222696030154122E-4 94-95 2.5781568241232977E-4 0.0 0.0 6.618740879770234 3.8672352361849465E-4 96-97 2.5781568241232977E-4 0.0 0.0 7.124349659941116 3.8672352361849465E-4 98-99 2.5781568241232977E-4 0.0 0.0 7.6598328323115235 3.8672352361849465E-4 100-101 2.5781568241232977E-4 0.0 0.0 8.267246580074973 3.8672352361849465E-4 102-103 2.9004264271387096E-4 0.0 0.0 8.898411597580658 4.511774442215771E-4 104-105 4.511774442215771E-4 0.0 0.0 9.5307690126175 4.511774442215771E-4 106-107 4.511774442215771E-4 0.0 0.0 10.1643510521458 4.511774442215771E-4 108-109 4.511774442215771E-4 0.0 0.0 10.811822911564064 4.511774442215771E-4 110-111 4.511774442215771E-4 0.0 0.0 11.51430619221706 4.511774442215771E-4 112-113 4.511774442215771E-4 0.0 0.0 12.269319418161569 4.511774442215771E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1615 0.0 58.208794 1 GTATCAA 2695 0.0 40.842937 1 GTACATG 6895 0.0 38.227287 1 TACATGG 7120 0.0 36.09539 2 ACATGGG 7055 0.0 34.993313 3 TCAACGC 2985 0.0 34.873837 4 ATCAACG 3035 0.0 34.497524 3 CAACGCA 3080 0.0 33.6083 5 AACGCAG 3260 0.0 31.740347 6 TATCAAC 3695 0.0 28.819511 2 CATGGGG 2890 0.0 27.169527 4 CATGGGA 4545 0.0 26.699446 4 ACGCAGA 3860 0.0 26.652552 7 CGCAGAG 3930 0.0 26.026508 8 ATGGGTA 775 0.0 24.563839 5 GAGTACA 4885 0.0 23.019783 1 TCTTACG 160 2.61464E-4 22.308958 2 GCAGAGT 4555 0.0 21.542175 9 GTCTTAC 415 1.8189894E-12 21.505384 1 CAGAGTA 4525 0.0 21.027876 10-11 >>END_MODULE