FastQCFastQC Report
Thu 26 May 2016
SRR1364196_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364196_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1551496
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58120.3746061865451151No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55570.35817043679132915No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31950.20593027632684843No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33400.071.823821
GTATCAA59550.048.4808121
TCAACGC73350.038.9400984
ATCAACG73600.038.6461263
CAACGCA74250.038.3817675
AACGCAG77000.037.2415926
TATCAAC79250.035.968322
GTACATG67600.034.606351
ATACCGA902.3948347E-433.0521246
ACGCAGA89150.031.8980737
CGCAGAG90050.031.5132058
TACATGG74250.031.1771952
ACATGGG73000.030.567813
TGGTATC15850.030.4116272
GTGGTAT18400.028.1456551
GCAGAGT101450.028.030699
GAGTACT66950.027.32715412-13
CATGGGA44500.026.8776914
CAGAGTA99700.026.2253710-11
GTACTTT71650.025.45154814-15
Produced by FastQC (version 0.11.5)