FastQCFastQC Report
Thu 26 May 2016
SRR1364195_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364195_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1498845
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27080.18067245112069627No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19350.047.657821
GTATCAA30900.040.048691
TACATGG75000.034.740682
GTACATG77200.034.6799661
ATCAACG32900.034.5329743
TCAACGC33900.033.6908954
CAACGCA34100.033.494415
ACATGGG75900.032.83743
AACGCAG36450.031.6518616
TATCAAC40700.029.6706522
CATGGGG28050.027.3569584
CATGGGA53500.027.3522014
ACGCAGA43200.026.4309357
CGCAGAG43700.025.992448
TAGGCCG1401.0576424E-425.4946295
ACCGTAC1350.002563235322.025788
ATGGGAT21400.021.6823485
GCAGAGT53050.021.1878059
ATGGGAG23250.020.7246675
GAGTACA54600.020.0497361
Produced by FastQC (version 0.11.5)