##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364195_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1498845 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.353831783806864 31.0 31.0 34.0 26.0 34.0 2 30.39617638915298 31.0 31.0 34.0 26.0 34.0 3 30.543931493916983 33.0 31.0 34.0 26.0 34.0 4 33.84718032885322 37.0 35.0 37.0 28.0 37.0 5 34.037686351824235 37.0 35.0 37.0 30.0 37.0 6 34.063426171485375 37.0 35.0 37.0 30.0 37.0 7 33.93346410069086 37.0 35.0 37.0 29.0 37.0 8 33.92998008466519 37.0 35.0 37.0 29.0 37.0 9 35.312954975331 39.0 37.0 39.0 28.0 39.0 10-11 35.29933882422799 39.0 36.0 39.0 28.0 39.0 12-13 35.26616227828762 39.0 36.0 39.0 28.0 39.0 14-15 36.49845614456465 40.0 37.0 41.0 27.5 41.0 16-17 36.54762500458686 40.0 37.0 41.0 28.5 41.0 18-19 36.43900236515451 40.0 37.0 41.0 27.5 41.0 20-21 36.413666523222886 40.0 37.0 41.0 27.0 41.0 22-23 36.45517014768038 40.0 37.0 41.0 27.5 41.0 24-25 36.34182687335915 40.0 36.0 41.0 27.0 41.0 26-27 36.36128185369401 40.0 36.0 41.0 27.5 41.0 28-29 36.45242703548399 40.0 36.0 41.0 29.0 41.0 30-31 36.35592639665876 40.0 36.0 41.0 28.5 41.0 32-33 36.17459910797981 40.0 36.0 41.0 27.5 41.0 34-35 36.134831486911594 40.0 36.0 41.0 27.0 41.0 36-37 36.159144874887 40.0 36.0 41.0 28.0 41.0 38-39 36.00301198589581 40.0 35.5 41.0 27.5 41.0 40-41 36.00474198466152 40.0 35.0 41.0 27.0 41.0 42-43 35.94387945384613 40.0 35.0 41.0 27.0 41.0 44-45 35.9348294853704 40.0 35.0 41.0 27.0 41.0 46-47 35.87276869856456 40.0 35.0 41.0 27.0 41.0 48-49 35.80384996447265 40.0 35.0 41.0 27.0 41.0 50-51 35.70452348308197 40.0 35.0 41.0 27.0 41.0 52-53 35.57822556701994 39.5 35.0 41.0 27.0 41.0 54-55 35.420017746998525 39.0 35.0 41.0 27.0 41.0 56-57 35.24833555170815 39.0 35.0 41.0 26.5 41.0 58-59 35.03908476193335 39.0 34.0 41.0 26.0 41.0 60-61 34.82078700599462 38.5 34.0 40.5 26.0 41.0 62-63 34.54994712595365 38.0 34.0 40.0 26.0 41.0 64-65 34.27656228629378 38.0 34.0 40.0 25.0 41.0 66-67 33.993261811594934 37.0 33.5 40.0 25.0 41.0 68-69 33.77367106004957 37.0 33.5 40.0 24.5 41.0 70-71 33.62766430151217 36.5 33.5 39.0 25.0 41.0 72-73 33.31446380379559 36.0 33.0 39.0 24.5 41.0 74-75 32.974280862931124 35.5 33.0 39.0 24.0 40.5 76-77 32.62177943683303 35.0 33.0 37.5 23.5 39.5 78-79 32.25727276669703 35.0 33.0 37.0 22.5 39.0 80-81 31.9219946025106 35.0 32.5 36.5 22.0 39.0 82-83 31.62097281573478 35.0 32.0 36.0 20.5 38.0 84-85 31.3333683603041 35.0 32.0 36.0 20.0 37.0 86-87 31.093973693077004 35.0 32.0 35.5 20.0 37.0 88-89 30.89334854504635 35.0 32.0 35.0 18.5 36.0 90-91 30.714307683583026 35.0 32.0 35.0 18.0 36.0 92-93 30.56481624183955 35.0 32.0 35.0 17.0 36.0 94-95 30.423449389363142 35.0 32.0 35.0 13.0 36.0 96-97 30.315143660618677 35.0 32.0 35.0 10.5 35.0 98-99 30.233152193855936 35.0 31.5 35.0 8.5 35.0 100-101 30.128884240865467 35.0 31.0 35.0 7.0 35.0 102-103 29.175265954785186 33.5 29.5 34.5 6.0 35.0 104-105 29.840767057300788 34.5 31.0 35.0 3.5 35.0 106-107 29.943468470722458 35.0 31.0 35.0 2.0 35.0 108-109 29.913926389986955 35.0 31.0 35.0 2.0 35.0 110-111 29.843966520887747 35.0 31.0 35.0 2.0 35.0 112-113 29.980068319272505 35.0 32.0 35.0 2.0 35.0 114-115 29.966436489430194 35.0 32.0 35.0 2.0 35.0 116-117 29.87249648896317 35.0 32.0 35.0 2.0 35.0 118-119 29.641049274608115 35.0 31.0 35.0 2.0 35.0 120-121 29.57224896503641 35.0 31.0 35.0 2.0 35.0 122-123 29.43728704435749 35.0 31.0 35.0 2.0 35.0 124-125 28.581570809523335 34.5 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.70245503873684 1101 2 0.7352809624615233 1101 3 0.7591197138504704 1101 4 0.8045063280759024 1101 5 0.5858355194972731 1101 6 0.629639629975145 1101 7 0.6456781037988293 1101 8 0.6828377665344121 1101 9 0.7916247777360397 1101 10-11 0.8813065311020054 1101 12-13 0.7507419253568912 1101 14-15 1.0085530730437284 1101 16-17 0.9747495308546519 1101 18-19 0.969159197186535 1101 20-21 1.0290749840774893 1101 22-23 1.0292481283051558 1101 24-25 1.2905298196396586 1101 26-27 1.203395446549493 1101 28-29 1.1648452337640123 1101 30-31 1.162613668983198 1101 32-33 1.4285033961110187 1101 34-35 1.3569551058406475 1101 36-37 1.181016392428532 1101 38-39 1.2138297864341823 1101 40-41 1.0835373077029118 1101 42-43 1.10396856502895 1101 44-45 1.1262582509641987 1101 46-47 0.9337328412536365 1101 48-49 0.87959714383652 1101 50-51 0.755905787799243 1101 52-53 0.7891013861799863 1101 54-55 0.8433846709061896 1101 56-57 0.7130309764885112 1101 58-59 0.8193695739360933 1101 60-61 0.7822991032047497 1101 62-63 0.7268496326051377 1101 64-65 0.7068945219683442 1101 66-67 0.6798728115574519 1101 68-69 0.591925032371023 1101 70-71 0.595192321359356 1101 72-73 0.6449663312821698 1101 74-75 0.6144489371537816 1101 76-77 0.5771311927483396 1101 78-79 0.6411713947610593 1101 80-81 0.6542850546027523 1101 82-83 0.6854789734261644 1101 84-85 0.6583395968274779 1101 86-87 0.7208200149880284 1101 88-89 0.6234111416279156 1101 90-91 0.5729888749659153 1101 92-93 0.5161835211570178 1101 94-95 0.5613493036004584 1101 96-97 0.5407347280317758 1101 98-99 0.5968632103689444 1101 100-101 0.5857816594017393 1101 102-103 0.5875510398005375 1101 104-105 0.6595366334973392 1101 106-107 0.615741465736587 1101 108-109 0.661618904147538 1101 110-111 0.5663078779102015 1101 112-113 0.6467067620686855 1101 114-115 0.7017933892151866 1101 116-117 0.6728604214242893 1101 118-119 0.5861786602777208 1101 120-121 0.5569840958456709 1101 122-123 0.5663818094574182 1101 124-125 0.4392663035003963 1102 1 0.4886547025787742 1102 2 0.536777720997037 1102 3 0.4785958274550879 1102 4 0.6278872318807132 1102 5 0.5440597568744678 1102 6 0.5094832970293766 1102 7 0.6035887218818274 1102 8 0.6584210409247433 1102 9 0.7547157593205185 1102 10-11 0.7382141737742174 1102 12-13 0.7561233188259564 1102 14-15 0.8743781667270412 1102 16-17 0.8147573797001897 1102 18-19 0.8107086939425656 1102 20-21 0.8428656189453889 1102 22-23 0.8732873440980171 1102 24-25 1.0949875624714167 1102 26-27 1.1694049692857362 1102 28-29 1.0259383349967663 1102 30-31 1.1716301191514944 1102 32-33 1.257615378897917 1102 34-35 1.2989022927696752 1102 36-37 1.096991824747498 1102 38-39 1.2058633336628404 1102 40-41 1.184012984540388 1102 42-43 1.1187543557332305 1102 44-45 0.9636817722858169 1102 46-47 0.9773397223954845 1102 48-49 0.9803306157338199 1102 50-51 0.9467292245272816 1102 52-53 0.9241483632140586 1102 54-55 0.9461613725051663 1102 56-57 0.8355370497261418 1102 58-59 0.9254023755871472 1102 60-61 0.9950664819475108 1102 62-63 0.9247050411475826 1102 64-65 0.8558360882123708 1102 66-67 0.9920418255948462 1102 68-69 1.0312146094010401 1102 70-71 1.037121422909074 1102 72-73 0.9969810948059106 1102 74-75 0.8801518029441695 1102 76-77 0.902099532880591 1102 78-79 0.9684536180458707 1102 80-81 0.9905769329711518 1102 82-83 0.9278621538979692 1102 84-85 0.9627912763528848 1102 86-87 0.9666253046583009 1102 88-89 0.9204508472941164 1102 90-91 0.9061054625203724 1102 92-93 0.9452740942001014 1102 94-95 0.9810679912751574 1102 96-97 0.9069357467090562 1102 98-99 0.9636943947480248 1102 100-101 0.9981724231880769 1102 102-103 0.855454572178278 1102 104-105 0.9920043427321126 1102 106-107 0.9855863224533365 1102 108-109 0.8976747108192207 1102 110-111 0.8853302053040757 1102 112-113 0.9432552353804127 1102 114-115 0.9676704182536149 1102 116-117 1.0121311603887087 1102 118-119 0.9402531707859509 1102 120-121 0.9542172209845781 1102 122-123 1.092694259160723 1102 124-125 0.9911185495058774 1103 1 0.0800801448263293 1103 2 -0.20371107312491787 1103 3 0.10528011916697011 1103 4 0.1517656912805947 1103 5 0.05146789234392912 1103 6 0.1396608379343931 1103 7 0.15767501105425907 1103 8 0.26330577272785405 1103 9 0.24432427069288565 1103 10-11 0.21520656946317018 1103 12-13 0.2934706960306741 1103 14-15 0.36720341600842943 1103 16-17 0.14568121021893177 1103 18-19 0.30514276350265135 1103 20-21 0.37744036123928737 1103 22-23 0.4090729390335639 1103 24-25 0.5421167394767537 1103 26-27 0.42225452989656986 1103 28-29 0.34392229994993784 1103 30-31 0.4063914087752494 1103 32-33 0.6316291352590824 1103 34-35 0.5792789177874695 1103 36-37 0.3865884011025287 1103 38-39 0.6177685636075054 1103 40-41 0.5251441634192204 1103 42-43 0.4625483473045051 1103 44-45 0.3617909245385249 1103 46-47 0.3085212088768188 1103 48-49 0.247509819517731 1103 50-51 0.16523321523602164 1103 52-53 0.215875490605832 1103 54-55 0.15253808177015316 1103 56-57 0.19298289583863237 1103 58-59 0.21459867753706874 1103 60-61 0.20368346417336625 1103 62-63 0.20109627302190347 1103 64-65 0.17888451228861157 1103 66-67 0.19689135411150716 1103 68-69 0.2046213848802978 1103 70-71 0.2316026307729473 1103 72-73 0.23219862303373162 1103 74-75 0.21387103517152184 1103 76-77 0.27048457459297737 1103 78-79 0.2075494251438954 1103 80-81 0.24345557891835767 1103 82-83 0.24224445155930852 1103 84-85 0.2132065948974926 1103 86-87 0.24908454005495884 1103 88-89 0.24828110125924852 1103 90-91 0.3141216821086452 1103 92-93 0.3411156934523092 1103 94-95 0.2988784471527026 1103 96-97 0.265452534965803 1103 98-99 0.30145909638262225 1103 100-101 0.39202518719768165 1103 102-103 0.4136969553304475 1103 104-105 0.4390946055632625 1103 106-107 0.36828165054595985 1103 108-109 0.3040622518646501 1103 110-111 0.31496334894752565 1103 112-113 0.3924054420868899 1103 114-115 0.31369886571847516 1103 116-117 0.3494631925711289 1103 118-119 0.07180574604175405 1103 120-121 0.08715405766339401 1103 122-123 0.15729349331372688 1103 124-125 0.04111002449349144 1104 1 0.4823274088769871 1104 2 0.5415707048024139 1104 3 0.5028137497392429 1104 4 0.5668854918296944 1104 5 0.6805003107625467 1104 6 0.49884911560552325 1104 7 0.4859514585645641 1104 8 0.6132778587792913 1104 9 0.7449857930355179 1104 10-11 0.7344052479480183 1104 12-13 0.6779369660388568 1104 14-15 0.709988998645187 1104 16-17 0.7463312610758521 1104 18-19 0.9624246714401536 1104 20-21 1.0177542210883175 1104 22-23 0.9876285260294821 1104 24-25 1.1916355106512029 1104 26-27 1.1400364798961817 1104 28-29 0.8826720227223745 1104 30-31 0.9150609841008475 1104 32-33 1.1246728263243213 1104 34-35 1.0519232481968501 1104 36-37 0.8489382202354321 1104 38-39 1.0833862266364278 1104 40-41 1.0496495511936743 1104 42-43 0.9895440034352418 1104 44-45 0.8596168868049858 1104 46-47 0.9527105189816609 1104 48-49 0.87382177510014 1104 50-51 0.834862265626434 1104 52-53 0.8117423143043894 1104 54-55 0.7198447298785027 1104 56-57 0.6901100534339051 1104 58-59 0.6814206553741613 1104 60-61 0.6300567922043143 1104 62-63 0.7797805318492124 1104 64-65 0.6956996072342747 1104 66-67 0.6490934969850386 1104 68-69 0.6906223484850926 1104 70-71 0.686437288425573 1104 72-73 0.7105148383611635 1104 74-75 0.7061333268684677 1104 76-77 0.5294587178641876 1104 78-79 0.6284978875824336 1104 80-81 0.5646759155682304 1104 82-83 0.5353009524502639 1104 84-85 0.6435553663013742 1104 86-87 0.6563327983010439 1104 88-89 0.6582836632243918 1104 90-91 0.6279593529381877 1104 92-93 0.6662420709865522 1104 94-95 0.7406444059012394 1104 96-97 0.6685454387047436 1104 98-99 0.648374605066536 1104 100-101 0.7463464958647705 1104 102-103 0.6992375299754059 1104 104-105 0.7248923326795591 1104 106-107 0.7991095476696799 1104 108-109 0.7033278855877576 1104 110-111 0.722163618145057 1104 112-113 0.7958205033828634 1104 114-115 0.7930402494855606 1104 116-117 0.7570320788428191 1104 118-119 0.6820771231553735 1104 120-121 0.6602775137926074 1104 122-123 0.7989879464680065 1104 124-125 0.86410729427989 1105 1 0.4966109248145649 1105 2 0.5259002766914129 1105 3 0.5123873884874861 1105 4 0.5063905778252789 1105 5 0.4201924014184115 1105 6 0.4010739555111016 1105 7 0.38170143786438615 1105 8 0.39251627956633683 1105 9 0.4589038140669004 1105 10-11 0.4330024005403814 1105 12-13 0.48837153706078595 1105 14-15 0.5295041144646575 1105 16-17 0.6086953624301898 1105 18-19 0.7348283218586147 1105 20-21 0.7620589371365085 1105 22-23 0.6574528166381626 1105 24-25 0.8583553598601412 1105 26-27 0.843446229447423 1105 28-29 0.8521894429016896 1105 30-31 0.8461373857374923 1105 32-33 0.9979959163224592 1105 34-35 0.9838985454592688 1105 36-37 0.8823760439198836 1105 38-39 0.8638729074365088 1105 40-41 0.8997036841216541 1105 42-43 0.741658351238911 1105 44-45 0.6489345385138847 1105 46-47 0.6268205283397066 1105 48-49 0.5599416002499993 1105 50-51 0.4045115420574419 1105 52-53 0.42291322594039116 1105 54-55 0.3935403450332231 1105 56-57 0.3881244763831404 1105 58-59 0.4593672676527305 1105 60-61 0.42901506532486167 1105 62-63 0.4839770835895081 1105 64-65 0.3736862138261543 1105 66-67 0.39454414384619696 1105 68-69 0.4058745548120086 1105 70-71 0.48683852043789955 1105 72-73 0.4267989292169929 1105 74-75 0.37382491290857445 1105 76-77 0.3621329001288984 1105 78-79 0.44888319217193384 1105 80-81 0.28887841177579787 1105 82-83 0.4102767371433771 1105 84-85 0.3984575989526675 1105 86-87 0.33928979191601627 1105 88-89 0.3457685286575902 1105 90-91 0.30785135266947705 1105 92-93 0.2997281690569835 1105 94-95 0.28168290667690954 1105 96-97 0.28343359523248424 1105 98-99 0.32770359891271994 1105 100-101 0.37648502304293885 1105 102-103 0.3418403005189674 1105 104-105 0.32762987312302627 1105 106-107 0.4142617291563724 1105 108-109 0.25851437469633964 1105 110-111 0.26182477569921403 1105 112-113 0.34566146349634863 1105 114-115 0.3464935865891867 1105 116-117 0.4099443210083855 1105 118-119 0.3032413404307306 1105 120-121 0.37021016399391016 1105 122-123 0.2973653256219073 1105 124-125 0.26537680127187 1106 1 0.18027380345552757 1106 2 0.26235879587071764 1106 3 0.2893621392922796 1106 4 0.2621036445746867 1106 5 0.22356206359891928 1106 6 0.226917340687379 1106 7 0.12928123850822004 1106 8 0.18146791436446108 1106 9 0.34786708443885317 1106 10-11 0.3693009305795556 1106 12-13 0.32649898537945177 1106 14-15 0.42870901691976826 1106 16-17 0.4548951840180564 1106 18-19 0.5151921274669604 1106 20-21 0.6075295960978409 1106 22-23 0.45992731533885944 1106 24-25 0.7044349471851206 1106 26-27 0.6473893314914037 1106 28-29 0.6405133384918074 1106 30-31 0.7739991551204213 1106 32-33 0.9005336349742876 1106 34-35 0.8129603303562973 1106 36-37 0.8079601497260356 1106 38-39 0.8116303445074422 1106 40-41 0.7830642317693233 1106 42-43 0.6788830729547755 1106 44-45 0.7135775203236108 1106 46-47 0.698210785485891 1106 48-49 0.6201935104233627 1106 50-51 0.6171660043000102 1106 52-53 0.518150979498941 1106 54-55 0.5300537453357492 1106 56-57 0.4995492523983458 1106 58-59 0.45704694030525417 1106 60-61 0.4823525357511045 1106 62-63 0.43376667362946364 1106 64-65 0.5061267754365133 1106 66-67 0.5389712857614057 1106 68-69 0.5530806596097477 1106 70-71 0.5412096176725143 1106 72-73 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 36162.0 3 6686.0 4 4742.0 5 3927.0 6 4083.0 7 4514.0 8 4513.0 9 4933.0 10 5565.0 11 6213.0 12 6542.0 13 6467.0 14 6516.0 15 6279.0 16 6046.0 17 5500.0 18 5138.0 19 4728.0 20 4715.0 21 4592.0 22 5179.0 23 7232.0 24 11359.0 25 16224.0 26 18204.0 27 17172.0 28 19129.0 29 23087.0 30 27071.0 31 32963.0 32 41434.0 33 54765.0 34 75955.0 35 116308.0 36 224340.0 37 452441.0 38 217339.0 39 782.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.12032342076679 19.41052018776083 12.695687122253505 23.77346926921888 2 15.286688405966403 21.253425274772955 40.37415181685586 23.085734502404783 3 18.770704799208087 26.51102064355672 29.166908632887516 25.551365924347675 4 11.881906503753 17.534467855033093 38.122131850600915 32.46149379061299 5 12.747538961437721 39.90306698670626 34.42216656832302 12.927227483533002 6 30.496253263507235 38.24106551301033 18.10930422326026 13.153377000222182 7 27.359309438839 33.82251195758851 21.46601469537422 17.35216390819828 8 24.655651518335787 37.49367012599702 20.289356137559253 17.561322218107943 9 25.866793343336695 17.654315468942755 19.770100525143295 36.70879066257725 10-11 23.618700991961887 28.665847120775155 28.2362360492328 19.479215838030154 12-13 24.116329931959175 26.271945802191876 27.940173711607773 21.671550554241175 14-15 21.65785141943607 27.669979028897153 25.60382831052178 25.068341241145 16-17 21.662261462621153 30.374924397624625 26.3985313540106 21.564282785743618 18-19 21.50587998237223 29.541427762670963 27.865455141093886 21.08723711386292 20-21 22.782151723097957 28.49386188993805 27.648768724847127 21.075217662116867 22-23 22.303123926077426 28.64903459573801 27.259966835602683 21.787874642581883 24-25 21.87625413708863 28.505970985251768 27.791594483414784 21.826180394244822 26-27 21.981179131891214 28.61505969728464 27.538423297705695 21.865337873118456 28-29 21.776344278297586 28.75470144608041 27.587788683968746 21.88116559165326 30-31 22.054244195628197 28.52881184941227 27.64076648333501 21.776177471624518 32-33 21.67743915559255 28.574612065298798 27.667854837445226 22.080093941663428 34-35 22.037397134857102 28.66911256376104 27.60742951251999 21.686060788861873 36-37 22.084352343361616 28.463227222832053 27.495987908674458 21.956432525131873 38-39 21.88388943853368 28.58748333686478 27.6956906921687 21.832936532432843 40-41 22.050056413715705 28.483722090890467 27.612228598651274 21.85399289674255 42-43 21.86068141096659 28.53114440626988 27.54279793423678 22.065376248526753 44-45 21.911904568898425 28.685674026022117 27.50826388798541 21.894157517094047 46-47 21.94526518898178 28.589409520962818 27.30582325894202 22.15950203111338 48-49 21.779944408637878 28.61640759170373 27.552552252010614 22.051095747647775 50-51 21.80643861339931 28.62601926253147 27.53949532848084 22.028046795588384 52-53 21.901113311736292 28.658244688392475 27.420434722996447 22.020207276874785 54-55 21.798208317083773 28.43520153513725 27.627199445407648 22.13939070237133 56-57 21.82316700515878 28.62699192155879 27.373992863643277 22.175848209639152 58-59 21.843471159190432 28.593499683397976 27.433315073392283 22.129714084019305 60-61 21.92108751479288 28.487007455911627 27.549404965069286 22.042500064226203 62-63 21.833904955093217 28.448180374467857 27.599522239867614 22.11839243057131 64-65 21.613123832399253 28.44038564184681 27.612022951694687 22.334467574059246 66-67 21.76287420994655 28.641685198269805 27.351044942223435 22.24439564956021 68-69 21.74852270786181 28.436405709527268 27.534850482555406 22.280221100055513 70-71 21.607495042714056 28.251325723710373 27.744821871305387 22.396357362270184 72-73 21.625157793322106 28.360397278882516 27.62083699182544 22.39360793596994 74-75 21.53586258040045 28.443337800164198 27.542465750682783 22.478333868752568 76-77 21.48623034228493 28.314515434946646 27.721189534032675 22.47806468873575 78-79 21.61146035300193 28.283422359991512 27.723375068888352 22.3817422181182 80-81 21.548298122398545 28.39293821959627 27.650177592014412 22.40858606599077 82-83 21.62318140513016 28.29038404827258 27.671698173632358 22.414736372964903 84-85 21.752404452457434 28.155214128718775 27.566261732006474 22.526119686817314 86-87 21.555390974508843 28.04711243665576 27.758439504788974 22.639057084046424 88-89 21.73665601044561 27.905877423545256 27.77574097876884 22.58172558724029 90-91 21.65032058338838 27.984961603383972 27.8860511198735 22.47866669335415 92-93 21.629380938258624 27.95550599025826 27.739100759036262 22.676012312446854 94-95 21.531379161954707 27.891843386073944 27.727049828367846 22.849727623603506 96-97 21.568777242318443 27.825978755244968 27.78624742046561 22.818996581970982 98-99 21.440783950477222 27.77764801509815 27.789994007188707 22.991574027235924 100-101 21.279991779097955 27.90295598814642 27.869525209542918 22.947527023212704 102-103 21.308674174906013 27.88927598834632 27.872962431780813 22.92908740496686 104-105 21.275736805299765 27.937445617069436 27.78971088299195 22.997106694638845 106-107 21.110879745961064 28.054870914931264 27.877609615357663 22.956639723750012 108-109 21.143661205029563 28.00993849820325 27.68107935720014 23.165320939567046 110-111 20.999292772266 28.193300018014288 27.754051547561065 23.053355662158644 112-113 21.100548582700128 28.229954977648973 27.731452719858478 22.938043719792418 114-115 20.989327341899802 28.290068851875866 27.649035296221907 23.07156851000243 116-117 20.946855208210955 28.148963751321677 27.817609819397422 23.086571221069946 118-119 20.92167691324976 28.153820607349417 27.66262740156384 23.261875077836983 120-121 20.84990170859387 28.198879284259547 27.714703098500483 23.236515908646105 122-123 20.92263218532321 28.105144341244646 27.710662877669375 23.26156059576277 124-125 20.848685271710067 28.164808370997875 27.69278855453176 23.293717802760302 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1942.0 1 1526.5 2 984.0 3 704.5 4 487.0 5 382.0 6 325.0 7 331.5 8 359.0 9 347.5 10 340.5 11 342.5 12 354.0 13 396.0 14 408.5 15 435.5 16 530.5 17 644.5 18 781.0 19 983.5 20 1329.5 21 1872.0 22 2466.5 23 3189.0 24 4297.5 25 5844.0 26 8097.5 27 10935.0 28 13633.5 29 16635.5 30 20436.5 31 24224.0 32 27403.0 33 30743.5 34 35127.5 35 39126.0 36 43111.5 37 46887.5 38 48620.0 39 49895.0 40 51745.0 41 53623.5 42 54955.0 43 56668.0 44 58378.5 45 59434.5 46 59210.0 47 58405.5 48 58298.0 49 57187.0 50 54947.0 51 52231.0 52 49074.0 53 46214.5 54 42746.5 55 38642.5 56 35009.5 57 31281.0 58 28274.5 59 24790.0 60 20108.5 61 15717.5 62 12238.0 63 9151.5 64 6550.0 65 4933.0 66 3630.5 67 2749.5 68 2082.5 69 1346.5 70 889.0 71 684.5 72 596.0 73 510.0 74 340.5 75 228.5 76 176.0 77 100.0 78 53.5 79 36.5 80 23.0 81 14.5 82 10.0 83 8.0 84 7.0 85 6.0 86 4.5 87 3.5 88 4.0 89 4.0 90 3.0 91 1.5 92 2.0 93 1.5 94 0.5 95 1.0 96 1.0 97 0.5 98 0.0 99 1.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0655171148450974 2 0.05270725125012927 3 0.04683606376910221 4 0.04736980808555921 5 0.04650247357131658 6 0.004937134927227298 7 4.003082373427539E-4 8 0.0 9 0.0010674886329140106 10-11 0.0031691068789634685 12-13 0.0026020035427279007 14-15 0.007739292588626576 16-17 0.0033025429580777195 18-19 0.004903775907448735 20-21 0.0089735763204334 22-23 0.01667950988928141 24-25 0.0037028511954204737 26-27 0.01617912459260297 28-29 0.006938676113941069 30-31 0.006938676113941069 32-33 0.0028355166811778404 34-35 0.018747769115552308 36-37 0.01667950988928141 38-39 0.02685401092174308 40-41 0.006404931797484062 42-43 0.0019014641273780812 44-45 3.0023117800706545E-4 46-47 0.002435208443835086 48-49 0.0034026200174134087 50-51 0.0019014641273780812 52-53 0.0019014641273780812 54-55 0.005771110421691369 56-57 0.002735439621842152 58-59 0.007872728667740827 60-61 0.015712098315703093 62-63 0.012342837318068246 64-65 0.0030023117800706545 66-67 0.0036027741360847854 68-69 0.010474732210468728 70-71 0.002735439621842152 72-73 0.003269183938299157 74-75 0.0013010017713639503 76-77 4.003082373427539E-4 78-79 0.002868875700956403 80-81 0.002968952760292092 82-83 0.00153451490981389 84-85 0.011141912606039984 86-87 0.006004623560141309 88-89 0.0022350543251637095 90-91 0.001000770593356885 92-93 0.002435208443835086 94-95 0.0 96-97 0.0018681051075995182 98-99 0.025553009150379127 100-101 0.015078276939910396 102-103 0.006104700619476997 104-105 0.014210942425667764 106-107 0.03282527546210582 108-109 0.0018013870680423927 110-111 0.002335131384499398 112-113 0.01707981812662417 114-115 0.03225817212587025 116-117 0.049971811628287115 118-119 0.3562076131954939 120-121 0.1178240578578839 122-123 0.01971518068913063 124-125 0.31584319926343285 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1498845.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.5006377463135 #Duplication Level Percentage of deduplicated Percentage of total 1 86.87793635385198 67.33095473507163 2 8.651712332321694 13.410264467051533 3 2.2630090766304356 5.261539399936643 4 0.8799946480176268 2.7280058573883506 5 0.414643089795242 1.6067551948116594 6 0.23975427490264953 1.1148665524416181 7 0.14249526819683409 0.7730431912760638 8 0.10309379323019327 0.6391867778421245 9 0.06849268178671485 0.47774038674831404 >10 0.3339551427121938 4.613981914343896 >50 0.018201637735017363 0.9596458987857314 >100 0.0065625519391063186 0.7709942934640951 >500 0.0 0.0 >1k 1.491488802648295E-4 0.31302133083834044 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2708 0.18067245112069627 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 6.671803955712565E-5 8 0.0 0.0 0.0 0.0 6.671803955712565E-5 9 0.0 0.0 0.0 0.0 6.671803955712565E-5 10-11 0.0 0.0 0.0 0.0 6.671803955712565E-5 12-13 0.0 0.0 0.0 0.0 6.671803955712565E-5 14-15 0.0 0.0 0.0 0.0 6.671803955712565E-5 16-17 0.0 0.0 0.0 0.0 6.671803955712565E-5 18-19 1.334360791142513E-4 0.0 0.0 1.0007705933568849E-4 6.671803955712565E-5 20-21 2.335131384499398E-4 0.0 0.0 2.0015411867137697E-4 6.671803955712565E-5 22-23 3.3359019778562824E-4 0.0 0.0 4.0030823734275395E-4 6.671803955712565E-5 24-25 3.3359019778562824E-4 0.0 0.0 7.338984351283822E-4 6.671803955712565E-5 26-27 3.3359019778562824E-4 0.0 0.0 0.0016012329493710156 6.671803955712565E-5 28-29 3.669492175641911E-4 0.0 0.0 0.0038362872745347247 6.671803955712565E-5 30-31 4.0030823734275395E-4 0.0 0.0 0.012809863594968125 6.671803955712565E-5 32-33 4.3366725712131674E-4 0.0 0.0 0.03282527546210582 6.671803955712565E-5 34-35 5.337443164570052E-4 0.0 0.0 0.06548375582531883 6.671803955712565E-5 36-37 5.337443164570052E-4 0.0 0.0 0.11478838705803468 6.671803955712565E-5 38-39 6.004623560141309E-4 0.0 0.0 0.1832410956436456 6.671803955712565E-5 40-41 6.671803955712565E-4 0.0 0.0 0.25376206345552743 1.334360791142513E-4 42-43 7.005394153498193E-4 0.0 0.0 0.3216476687049028 1.334360791142513E-4 44-45 7.338984351283822E-4 0.0 0.0 0.4110832007312297 1.334360791142513E-4 46-47 8.006164746855079E-4 0.0 0.0 0.5170981655875024 1.334360791142513E-4 48-49 8.006164746855079E-4 0.0 0.0 0.6319532706850941 1.334360791142513E-4 50-51 8.006164746855079E-4 0.0 0.0 0.7545143093515341 1.334360791142513E-4 52-53 8.006164746855079E-4 0.0 0.0 0.870270107983147 1.334360791142513E-4 54-55 8.006164746855079E-4 0.0 0.0 0.9992360784470709 1.334360791142513E-4 56-57 8.006164746855079E-4 0.0 0.0 1.1582251667117012 1.334360791142513E-4 58-59 8.006164746855079E-4 0.0 0.0 1.3281560134637003 1.334360791142513E-4 60-61 8.006164746855079E-4 0.0 0.0 1.517968836003723 1.334360791142513E-4 62-63 8.006164746855079E-4 0.0 0.0 1.7081819667810882 1.334360791142513E-4 64-65 8.006164746855079E-4 0.0 0.0 1.9058341589690728 1.334360791142513E-4 66-67 8.673345142426335E-4 0.0 0.0 2.134910547788464 1.334360791142513E-4 68-69 8.673345142426335E-4 0.0 0.0 2.3990806254149026 1.334360791142513E-4 70-71 8.673345142426335E-4 0.0 0.0 2.696176055562783 1.334360791142513E-4 72-73 8.673345142426335E-4 0.0 0.0 3.0020115488926473 1.334360791142513E-4 74-75 9.006935340211963E-4 0.0 0.0 3.30277647121617 1.334360791142513E-4 76-77 9.340525537997592E-4 0.0 0.0 3.6354993344875552 1.334360791142513E-4 78-79 9.340525537997592E-4 0.0 0.0 4.014891466429151 1.334360791142513E-4 80-81 9.340525537997592E-4 0.0 0.0 4.42434007519123 1.334360791142513E-4 82-83 9.340525537997592E-4 0.0 0.0 4.860242386637712 1.334360791142513E-4 84-85 9.67411573578322E-4 0.0 0.0 5.2880384562780005 1.334360791142513E-4 86-87 0.0010341296131354477 0.0 0.0 5.729978750304401 1.334360791142513E-4 88-89 0.0010674886329140104 0.0 0.0 6.225493630095173 1.334360791142513E-4 90-91 0.0010674886329140104 0.0 0.0 6.7712805526922395 1.334360791142513E-4 92-93 0.0010674886329140104 0.0 0.0 7.345789591318649 1.334360791142513E-4 94-95 0.0010674886329140104 0.0 0.0 7.914861109721151 1.334360791142513E-4 96-97 0.0010674886329140104 0.0 0.0 8.493373230720989 1.334360791142513E-4 98-99 0.001134206672471136 0.0 0.0 9.102542290897325 1.334360791142513E-4 100-101 0.001167565692249699 0.0 0.0 9.780464290837278 1.334360791142513E-4 102-103 0.0012009247120282618 0.0 0.0 10.489276743092182 1.334360791142513E-4 104-105 0.0013010017713639501 0.0 0.0 11.195787422982363 1.334360791142513E-4 106-107 0.0013677198109210758 0.0 0.0 11.894492092244361 1.334360791142513E-4 108-109 0.0014010788306996387 0.0 0.0 12.617749000063382 1.334360791142513E-4 110-111 0.0014010788306996387 0.0 0.0 13.385606917326342 1.334360791142513E-4 112-113 0.0014010788306996387 0.0 0.0 14.197432022657447 1.334360791142513E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1935 0.0 47.65782 1 GTATCAA 3090 0.0 40.04869 1 TACATGG 7500 0.0 34.74068 2 GTACATG 7720 0.0 34.679966 1 ATCAACG 3290 0.0 34.532974 3 TCAACGC 3390 0.0 33.690895 4 CAACGCA 3410 0.0 33.49441 5 ACATGGG 7590 0.0 32.8374 3 AACGCAG 3645 0.0 31.651861 6 TATCAAC 4070 0.0 29.670652 2 CATGGGG 2805 0.0 27.356958 4 CATGGGA 5350 0.0 27.352201 4 ACGCAGA 4320 0.0 26.430935 7 CGCAGAG 4370 0.0 25.99244 8 TAGGCCG 140 1.0576424E-4 25.494629 5 ACCGTAC 135 0.0025632353 22.02578 8 ATGGGAT 2140 0.0 21.682348 5 GCAGAGT 5305 0.0 21.187805 9 ATGGGAG 2325 0.0 20.724667 5 GAGTACA 5460 0.0 20.049736 1 >>END_MODULE