FastQCFastQC Report
Thu 26 May 2016
SRR1364195_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364195_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1498845
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58540.39056740356741354No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54570.3640803418632347No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32280.21536583169040163No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15340.10234547268063074No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34350.072.102681
GTATCAA60550.052.211381
ATCAACG76300.041.024483
TCAACGC76650.040.8371544
CAACGCA77550.040.3524485
TATCAAC78950.039.948972
AACGCAG79800.039.5861326
GTACATG75900.033.6511841
ACGCAGA94400.033.2116247
CGCAGAG94350.033.166178
TACATGG75650.032.4093362
ACATGGG73050.032.177983
TGGTATC15350.031.4020882
GTGGTAT16200.030.8708841
CGTTCGC400.00536620629.74700246-47
GCAGAGT106550.028.9219639
GAGTACT68350.027.06997312-13
CATGGGA53350.026.659094
CAGAGTA104300.025.9524610-11
ATGGGAG21850.025.0496885
Produced by FastQC (version 0.11.5)