FastQCFastQC Report
Thu 26 May 2016
SRR1364194_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364194_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3496987
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28000.046.957541
GTATCAA45000.042.306651
TCAACGC45000.040.187294
ATCAACG45650.039.8756983
CAACGCA45400.039.5728535
AACGCAG50450.035.3646776
TACATGG103500.034.3142172
GTACATG108050.033.7525671
TATCAAC56700.032.3152662
ACATGGG107350.031.808083
CGCAGAG59050.029.7093478
ACGCAGA60300.029.2911437
CATGGGG66850.027.1410144
GAGTACA76950.022.5759141
TATACCG2704.5563866E-822.033455
GTATAGG14800.021.3051831
AGTACAT77350.020.8424632
TACCGCG1500.00472158919.82337
CAGAGTA70600.019.62843710-11
GCAGAGT87650.019.406569
Produced by FastQC (version 0.11.5)