##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364194_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3496987 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.490943489352404 31.0 31.0 34.0 26.0 34.0 2 30.4740463719196 31.0 31.0 34.0 26.0 34.0 3 30.618311706620585 31.0 31.0 34.0 26.0 34.0 4 34.023020674655065 37.0 35.0 37.0 30.0 37.0 5 34.11884087644592 37.0 35.0 37.0 30.0 37.0 6 34.17016219963071 37.0 35.0 37.0 30.0 37.0 7 34.063429460847296 37.0 35.0 37.0 30.0 37.0 8 34.04633217109472 37.0 35.0 37.0 30.0 37.0 9 35.50828327357237 39.0 37.0 39.0 30.0 39.0 10-11 35.4399069827826 39.0 35.0 39.0 29.5 39.0 12-13 35.382794388426376 39.0 35.0 39.0 29.0 39.0 14-15 36.59958129669913 40.0 37.0 41.0 29.0 41.0 16-17 36.61437803457662 40.0 37.0 41.0 29.0 41.0 18-19 36.479292316499894 40.0 37.0 41.0 28.0 41.0 20-21 36.42554676354244 40.0 37.0 41.0 27.0 41.0 22-23 36.42299585328742 40.0 36.5 41.0 27.5 41.0 24-25 36.26103628638025 40.0 36.0 41.0 27.0 41.0 26-27 36.23882773942253 40.0 36.0 41.0 27.0 41.0 28-29 36.30995740047075 40.0 36.0 41.0 28.0 41.0 30-31 36.194511303587916 40.0 36.0 41.0 27.5 41.0 32-33 36.003852316294 40.0 36.0 41.0 27.0 41.0 34-35 35.95820287578993 40.0 36.0 41.0 27.0 41.0 36-37 35.98500266086205 40.0 36.0 41.0 27.0 41.0 38-39 35.82333320083832 40.0 35.5 41.0 26.5 41.0 40-41 35.830999657705334 40.0 35.0 41.0 27.0 41.0 42-43 35.7600168373517 40.0 35.0 41.0 27.0 41.0 44-45 35.73823208379099 40.0 35.0 41.0 27.0 41.0 46-47 35.66848246790737 40.0 35.0 41.0 27.0 41.0 48-49 35.57544780692636 40.0 35.0 41.0 27.0 41.0 50-51 35.45535756924461 39.0 35.0 41.0 27.0 41.0 52-53 35.313450979371666 39.0 35.0 41.0 26.0 41.0 54-55 35.139949905447175 39.0 35.0 41.0 26.0 41.0 56-57 34.95495093347502 39.0 34.0 41.0 26.0 41.0 58-59 34.7314842748915 38.5 34.0 40.0 26.0 41.0 60-61 34.495148394889654 38.0 34.0 40.0 25.0 41.0 62-63 34.21073355434264 38.0 34.0 40.0 24.0 41.0 64-65 33.91190602081163 37.0 33.0 40.0 23.0 41.0 66-67 33.61482113030446 37.0 33.0 40.0 23.0 41.0 68-69 33.39471307728624 36.0 33.0 39.0 23.0 41.0 70-71 33.24799777637149 36.0 33.0 39.0 23.0 41.0 72-73 32.93977072262493 35.5 33.0 39.0 23.0 40.5 74-75 32.60306572486543 35.0 33.0 37.5 22.0 40.0 76-77 32.26285184932057 35.0 32.5 37.0 22.0 39.0 78-79 31.91531281071391 35.0 32.0 37.0 21.0 39.0 80-81 31.60931910813509 35.0 32.0 36.0 20.0 39.0 82-83 31.32944446176094 35.0 32.0 36.0 20.0 37.0 84-85 31.069290506370198 35.0 32.0 35.5 19.0 37.0 86-87 30.854434832042557 35.0 32.0 35.0 18.0 36.5 88-89 30.67647020134762 35.0 32.0 35.0 18.0 36.0 90-91 30.517428288981343 35.0 32.0 35.0 16.5 36.0 92-93 30.382288095437588 35.0 31.0 35.0 13.5 36.0 94-95 30.24434205789155 35.0 31.0 35.0 10.5 35.0 96-97 30.14398738113696 35.0 31.0 35.0 8.5 35.0 98-99 30.06961893195485 35.0 31.0 35.0 7.0 35.0 100-101 29.965987291345378 35.0 31.0 35.0 7.0 35.0 102-103 28.9754068287929 33.5 29.5 34.5 2.0 35.0 104-105 29.67988213853812 34.0 30.5 35.0 2.0 35.0 106-107 29.79695549340046 34.0 31.0 35.0 2.0 35.0 108-109 29.768742348770527 35.0 31.0 35.0 2.0 35.0 110-111 29.698374486379272 34.0 31.0 35.0 2.0 35.0 112-113 29.85239393226226 35.0 31.0 35.0 2.0 35.0 114-115 29.844394617423514 35.0 32.0 35.0 2.0 35.0 116-117 29.751372824663058 35.0 31.5 35.0 2.0 35.0 118-119 29.519229124958144 35.0 31.0 35.0 2.0 35.0 120-121 29.44843918493263 35.0 31.0 35.0 2.0 35.0 122-123 29.31475982038252 35.0 31.0 35.0 2.0 35.0 124-125 28.424759800365287 34.5 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6520627241870365 1101 2 0.6619658870570682 1101 3 0.6672242964134405 1101 4 0.6158460552122804 1101 5 0.6314229470938386 1101 6 0.6859109666618863 1101 7 0.608426507004225 1101 8 0.6616012779768781 1101 9 0.7026621510582842 1101 10-11 0.8605763239624267 1101 12-13 0.8387183239020999 1101 14-15 0.9059681745434318 1101 16-17 0.9235635879328967 1101 18-19 1.0062315111099878 1101 20-21 1.11557481861621 1101 22-23 1.0761960464483025 1101 24-25 1.2757237915819672 1101 26-27 1.249055801962328 1101 28-29 1.1545358630111338 1101 30-31 1.2474496960570178 1101 32-33 1.3961259539408317 1101 34-35 1.341864184610614 1101 36-37 1.2460862828721915 1101 38-39 1.3469384896210173 1101 40-41 1.287806204373446 1101 42-43 1.1749642920676493 1101 44-45 1.0910737671744002 1101 46-47 0.9944685777566775 1101 48-49 0.9913651385437845 1101 50-51 0.9590995677200382 1101 52-53 0.9162729288463751 1101 54-55 0.9408912659420849 1101 56-57 0.9207546492935492 1101 58-59 0.948645412027922 1101 60-61 0.8595401953014061 1101 62-63 0.8499481324375964 1101 64-65 0.7927025887976029 1101 66-67 0.762545229481951 1101 68-69 0.7731106435099022 1101 70-71 0.7699023789327768 1101 72-73 0.6768700303954915 1101 74-75 0.7262573019460419 1101 76-77 0.6837882806781508 1101 78-79 0.6987559941398267 1101 80-81 0.6560404676136962 1101 82-83 0.696412444170889 1101 84-85 0.6678888271539201 1101 86-87 0.6856967177271045 1101 88-89 0.7468972763853365 1101 90-91 0.776600467708505 1101 92-93 0.7535715062690187 1101 94-95 0.7749422676979236 1101 96-97 0.6760706941906669 1101 98-99 0.674407354497859 1101 100-101 0.6388650150151314 1101 102-103 0.570357258709052 1101 104-105 0.6587040604942445 1101 106-107 0.738327470967878 1101 108-109 0.6794233379080978 1101 110-111 0.5914901123665466 1101 112-113 0.7005845681415579 1101 114-115 0.7231640793619825 1101 116-117 0.7177734824506956 1101 118-119 0.5143883463143162 1101 120-121 0.5097664236118575 1101 122-123 0.5580726283769657 1101 124-125 0.4765249412744872 1102 1 0.2511569348463212 1102 2 0.27420343765583155 1102 3 0.2453513859463179 1102 4 0.36398519511432426 1102 5 0.3081500485826041 1102 6 0.28918974050180424 1102 7 0.3560424136218501 1102 8 0.3284979951715883 1102 9 0.3304940000309031 1102 10-11 0.4101350146192857 1102 12-13 0.41531490823000894 1102 14-15 0.5545787049176738 1102 16-17 0.5023690401141252 1102 18-19 0.6161279297331035 1102 20-21 0.6514632507341673 1102 22-23 0.6149868168232899 1102 24-25 0.8112119903482338 1102 26-27 0.9255398966315127 1102 28-29 0.6572361401305287 1102 30-31 0.7336041509064941 1102 32-33 0.9332301938692495 1102 34-35 0.8643278286728844 1102 36-37 0.7551708082793667 1102 38-39 0.7716738107060124 1102 40-41 0.6835029129677253 1102 42-43 0.6730561947831504 1102 44-45 0.48988497414804044 1102 46-47 0.571114557521895 1102 48-49 0.47867953101410876 1102 50-51 0.33602617302288706 1102 52-53 0.36621456241579153 1102 54-55 0.36714864247670675 1102 56-57 0.3695235382635218 1102 58-59 0.4366367826083817 1102 60-61 0.4420372946938187 1102 62-63 0.4434722440079213 1102 64-65 0.44993659334964065 1102 66-67 0.40251896967839684 1102 68-69 0.4899851185052242 1102 70-71 0.5408566116371603 1102 72-73 0.5244203791280952 1102 74-75 0.5044942871645475 1102 76-77 0.45541130642843797 1102 78-79 0.5081716603690332 1102 80-81 0.4783331334215184 1102 82-83 0.47159157491218906 1102 84-85 0.5106001537028 1102 86-87 0.5221386719392846 1102 88-89 0.5431782153172655 1102 90-91 0.5095482709431423 1102 92-93 0.5807138429883913 1102 94-95 0.59255454275322 1102 96-97 0.5459129228020352 1102 98-99 0.5953553119863457 1102 100-101 0.517858349033677 1102 102-103 0.4081495245482145 1102 104-105 0.5226566670992092 1102 106-107 0.48574172366499724 1102 108-109 0.47606698513362034 1102 110-111 0.4903186464288751 1102 112-113 0.5489896248986668 1102 114-115 0.581828116437638 1102 116-117 0.539743998790609 1102 118-119 0.35990778107739985 1102 120-121 0.3776625819417845 1102 122-123 0.5294109564183742 1102 124-125 0.4639334619833484 1103 1 0.4705231220560222 1103 2 0.1247731613656029 1103 3 0.4510147343194646 1103 4 0.5444816962718377 1103 5 0.5474880243384348 1103 6 0.5519384480637015 1103 7 0.532251747345839 1103 8 0.5717513994057981 1103 9 0.6793279616870365 1103 10-11 0.6496341533550378 1103 12-13 0.6164007055963339 1103 14-15 0.6544591424947086 1103 16-17 0.6386489396196211 1103 18-19 0.7902375787561198 1103 20-21 0.9088641140260165 1103 22-23 0.8980283232749855 1103 24-25 1.109537590180203 1103 26-27 1.0798585575942852 1103 28-29 0.9925427235821473 1103 30-31 1.029433446459052 1103 32-33 1.2089236744958782 1103 34-35 1.1896971182602698 1103 36-37 0.9413819591014487 1103 38-39 1.1399566034836255 1103 40-41 1.0969720519402912 1103 42-43 0.997412808176648 1103 44-45 0.9155661847192178 1103 46-47 0.8349686728698629 1103 48-49 0.7901688959600008 1103 50-51 0.8272336006512333 1103 52-53 0.8414000875114809 1103 54-55 0.7586959788879568 1103 56-57 0.8037439877058645 1103 58-59 0.7580635188076812 1103 60-61 0.6740673709175269 1103 62-63 0.7210388331461033 1103 64-65 0.7086397618641342 1103 66-67 0.7380265328461562 1103 68-69 0.7912716672895925 1103 70-71 0.775873388064916 1103 72-73 0.7671875664537211 1103 74-75 0.7546086103396235 1103 76-77 0.7327691962791718 1103 78-79 0.769506312723653 1103 80-81 0.7791744428713123 1103 82-83 0.6810643285788487 1103 84-85 0.7194394381871483 1103 86-87 0.7364549632451833 1103 88-89 0.718152591757768 1103 90-91 0.6941422392918923 1103 92-93 0.692086110356886 1103 94-95 0.6492566513454179 1103 96-97 0.6533234829588963 1103 98-99 0.6030418768057473 1103 100-101 0.5799927915397767 1103 102-103 0.5436421632114445 1103 104-105 0.5482279695483783 1103 106-107 0.5947830480973373 1103 108-109 0.4499113169406854 1103 110-111 0.49406931333018633 1103 112-113 0.556368122715746 1103 114-115 0.55692359921537 1103 116-117 0.631856961154238 1103 118-119 0.5702139582357937 1103 120-121 0.6454668541653632 1103 122-123 0.7332614437613003 1103 124-125 0.6896360048836563 1104 1 0.5426433843610425 1104 2 0.5717450598188698 1104 3 0.5059630087740103 1104 4 0.5723841310461069 1104 5 0.5460054657625761 1104 6 0.55803027092837 1104 7 0.6469308271183891 1104 8 0.5997794368881983 1104 9 0.7778985948147366 1104 10-11 0.7906046328747607 1104 12-13 0.7939194316019496 1104 14-15 0.8148050203573263 1104 16-17 0.8014712518735863 1104 18-19 0.9540783309226839 1104 20-21 0.9895419073866805 1104 22-23 1.0822622364454872 1104 24-25 1.2160676131079171 1104 26-27 1.2333137852258602 1104 28-29 1.048794890329539 1104 30-31 1.0878967480274468 1104 32-33 1.17407362714318 1104 34-35 1.1849479374601017 1104 36-37 1.1096973489292807 1104 38-39 1.1880458388812585 1104 40-41 1.0916589402402792 1104 42-43 0.9791429772442655 1104 44-45 0.9692462781726121 1104 46-47 0.9242298243659093 1104 48-49 0.8551466436702526 1104 50-51 0.8436531803232867 1104 52-53 0.9084642459312349 1104 54-55 0.9040150357584196 1104 56-57 0.7944734571909251 1104 58-59 0.8418868711003924 1104 60-61 0.8179756578774331 1104 62-63 0.9109653385390359 1104 64-65 0.8785170036125507 1104 66-67 0.9276689037537764 1104 68-69 0.875866043026555 1104 70-71 0.8287682540954009 1104 72-73 0.8423980544079868 1104 74-75 0.8541051927818231 1104 76-77 0.7837051030219939 1104 78-79 0.8504005154136927 1104 80-81 0.8352310669589862 1104 82-83 0.8542639102204248 1104 84-85 0.8983870185503697 1104 86-87 0.9062085242155788 1104 88-89 0.8841504450533648 1104 90-91 0.8357136605939814 1104 92-93 0.8050695901779008 1104 94-95 0.8749020080172087 1104 96-97 0.8469822850875843 1104 98-99 0.8356810089865832 1104 100-101 0.8407176923224249 1104 102-103 0.8078427006929303 1104 104-105 0.8822207994678131 1104 106-107 0.9234972878421921 1104 108-109 0.892537822955962 1104 110-111 0.8151260893418595 1104 112-113 0.8843959771571832 1104 114-115 0.8317071258216551 1104 116-117 0.8423317913745905 1104 118-119 0.8117656111139873 1104 120-121 0.807580303685782 1104 122-123 0.8271662346388311 1104 124-125 0.7877461618632502 1105 1 0.41627499204743046 1105 2 0.37268936900443705 1105 3 0.3702854218804461 1105 4 0.418434521835124 1105 5 0.34943201058212026 1105 6 0.34888308664571355 1105 7 0.27710889427606844 1105 8 0.27868836331317226 1105 9 0.28059366043882505 1105 10-11 0.28516935860160686 1105 12-13 0.3271636202259032 1105 14-15 0.3102268328182447 1105 16-17 0.4217370460754566 1105 18-19 0.5666276413427056 1105 20-21 0.6196215789689177 1105 22-23 0.616543363056806 1105 24-25 0.9145588809511267 1105 26-27 0.8156821451013698 1105 28-29 0.7196008690321207 1105 30-31 0.7094811510672656 1105 32-33 0.8957880529076832 1105 34-35 0.8388627658174812 1105 36-37 0.7128034773877658 1105 38-39 0.7639110801937008 1105 40-41 0.729863365157783 1105 42-43 0.6935431516891626 1105 44-45 0.5991327628374989 1105 46-47 0.5228248040290282 1105 48-49 0.5455440644807297 1105 50-51 0.44336934693258456 1105 52-53 0.4418345827288377 1105 54-55 0.4499449550416159 1105 56-57 0.45176446120424174 1105 58-59 0.5201338800370863 1105 60-61 0.3729500407876003 1105 62-63 0.39412032238305983 1105 64-65 0.3947459661519517 1105 66-67 0.452205264162707 1105 68-69 0.4883672369289158 1105 70-71 0.5254086579137294 1105 72-73 0.5080323184144859 1105 74-75 0.47537350454081917 1105 76-77 0.4678808959083227 1105 78-79 0.46589214187813965 1105 80-81 0.36813213255063104 1105 82-83 0.4333835040953886 1105 84-85 0.45814845433283935 1105 86-87 0.45727123247269574 1105 88-89 0.4303739291577955 1105 90-91 0.38339280451643276 1105 92-93 0.4459058264209901 1105 94-95 0.45487850841457345 1105 96-97 0.45655530956213397 1105 98-99 0.4832120189108373 1105 100-101 0.517073199501155 1105 102-103 0.42974806072882643 1105 104-105 0.3520697640616248 1105 106-107 0.5138052335552281 1105 108-109 0.4912282247018531 1105 110-111 0.45550485824381326 1105 112-113 0.40648326088064124 1105 114-115 0.4743105177658329 1105 116-117 0.6083275600044225 1105 118-119 0.5637064007655113 1105 120-121 0.543096420917081 1105 122-123 0.5427002108472863 1105 124-125 0.5420669170222574 1106 1 0.48204156268440457 1106 2 0.5505059761284272 1106 3 0.6103864825478027 1106 4 0.5615428525088717 1106 5 0.5926119632539653 1106 6 0.5606079599729767 1106 7 0.5673018751779395 1106 8 0.5150722671852819 1106 9 0.6036749078214854 1106 10-11 0.663929860403897 1106 12-13 0.6905181697527141 1106 14-15 0.7574675713253711 1106 16-17 0.7371241588834891 1106 18-19 0.8240922688638364 1106 20-21 0.8988836482362501 1106 22-23 0.8662576400286355 1106 24-25 1.0204447112877517 1106 26-27 1.0095439655240668 1106 28-29 0.8790743637337641 1106 30-31 0.9334330700422555 1106 32-33 1.12541693140966 1106 34-35 1.1056393560624542 1106 36-37 0.9448930322817759 1106 38-39 1.0073729488625176 1106 40-41 0.8804329788579679 1106 42-43 0.9324869282427599 1106 44-45 0.8950063146090343 1106 46-47 0.7860334055001061 1106 48-49 0.7610884219090082 1106 50-51 0.6727839465058452 1106 52-53 0.6466208214852003 1106 54-55 0.6669740368461703 1106 56-57 0.665528337744 1106 58-59 0.6816926493911311 1106 60-61 0.6545189041112707 1106 62-63 0.6738288816921596 1106 64-65 0.6687823514078559 1106 66-67 0.675812219857761 1106 68-69 0.684469744268128 1106 70-71 0.7080448597916984 1106 72-73 0.6966572827902766 1106 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2216 118-119 -3.383382987004129 2216 120-121 -3.279120416970841 2216 122-123 -3.2315393518376965 2216 124-125 -3.0453598287367605 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 91076.0 3 16172.0 4 10554.0 5 8797.0 6 8988.0 7 9808.0 8 10522.0 9 11305.0 10 12625.0 11 13944.0 12 14733.0 13 14736.0 14 14683.0 15 14241.0 16 13266.0 17 12560.0 18 11865.0 19 11250.0 20 11455.0 21 11887.0 22 13789.0 23 17822.0 24 24633.0 25 33792.0 26 39549.0 27 42863.0 28 49998.0 29 59080.0 30 70134.0 31 83859.0 32 105850.0 33 138268.0 34 192170.0 35 292131.0 36 566729.0 37 1027521.0 38 413240.0 39 1092.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.19142726336271 18.46789515852123 12.25163099462058 25.089046583495477 2 15.570803677364035 20.247017284457836 39.822489050048624 24.359689988129507 3 18.669211724431122 24.76187288329068 29.297374093165825 27.27154129911238 4 12.184771774593152 16.482501021341168 38.033886783902524 33.29884042016316 5 13.346882422174275 38.23721090175251 35.14166178888025 13.274244887192966 6 32.14248537821047 37.06845273841839 17.73583319320666 13.053228690164481 7 28.09725099314493 32.067600198114256 22.02762847400551 17.807520334735308 8 25.434438275006453 36.22252527675968 20.399332339525426 17.943704108708438 9 26.248851500148845 15.820201186575854 19.589940156473364 38.341007156801936 10-11 24.52605269592248 26.653492924356314 28.78653818931583 20.033916190405378 12-13 25.0550487259866 24.040493556868338 28.287281141993688 22.617176575151376 14-15 22.209603988442257 25.768827697286888 25.529802569763206 26.49176574450765 16-17 22.702219957353552 28.43198023158568 26.270674969469315 22.595124841591456 18-19 22.617211087268824 27.90461280337015 27.406331721536258 22.071844387824772 20-21 23.345290970684935 27.31773659327307 27.12969944514027 22.207272990901718 22-23 23.002475254250182 27.18087717725203 26.883886708317977 22.93276086017981 24-25 22.70653532427827 27.15286018292668 27.369439859782997 22.77116463301205 26-27 22.682227333796188 27.347381502092492 27.10775085104846 22.86264031306285 28-29 22.748557578877378 27.530718029012863 26.99992135498216 22.7208030371276 30-31 23.052425537439795 27.261130758773778 26.93851602656817 22.747927677218254 32-33 22.72751125032654 27.204549974784797 27.180357107877168 22.887581667011496 34-35 23.06827886777537 27.43381789746872 26.901403697239797 22.59649953751611 36-37 23.128760529482552 27.235632319514245 26.824456070580517 22.811151080422686 38-39 22.823686899637952 27.445579926588948 26.987879953690825 22.742853220082278 40-41 23.03311253543431 27.23149462184934 26.935279881891596 22.800112960824755 42-43 22.894784741118702 27.157339394250492 26.862910592845374 23.08496527178544 44-45 22.947499240773034 27.337389597532496 26.880094756100835 22.83501640559363 46-47 22.936273568496286 27.404763378853065 26.639701896689605 23.019261155961043 48-49 22.82854473541151 27.272316517309847 26.967759573311433 22.931379173967215 50-51 22.858553076518987 27.36314182497012 26.8861367550844 22.89216834342649 52-53 22.897026820668415 27.378043026311648 26.806641825830084 22.91828832718985 54-55 22.843058106704117 27.156470032095143 26.880517022607297 23.119954838593443 56-57 22.743064640986198 27.38447693227628 26.86104174012461 23.01141668661291 58-59 22.788268885280427 27.257388213747863 26.997816237969435 22.95652666300228 60-61 22.927176450248247 27.241287542244702 26.858312780311543 22.973223227195515 62-63 22.78153909688874 27.261631942508014 26.962823714871515 22.99400524573173 64-65 22.690393362130227 27.124757464100398 26.912612724680823 23.272236449088552 66-67 22.674323835081474 27.28278046495507 26.812800877588828 23.230094822374625 68-69 22.740488678330927 27.121399985786336 26.99818153765241 23.139929798230327 70-71 22.62993019687544 26.87970067258018 26.953723240812987 23.536645889731396 72-73 22.605804880629474 27.118649884111147 26.937989096009733 23.337556139249646 74-75 22.57767621631638 27.206035427423654 26.83834379196512 23.377944564294847 76-77 22.643325557570787 27.07651107277642 26.918002760955712 23.36216060869708 78-79 22.73212314318858 27.063645294952927 26.938005029328217 23.266226532530276 80-81 22.73242989466237 26.911504580416047 26.98146657673936 23.374598948182225 82-83 22.733844714344215 27.022198862089553 27.037383583800217 23.20657283976601 84-85 22.782222257812382 26.978001754277088 26.888915136631415 23.350860851279116 86-87 22.63628383511515 26.869879807152657 27.123743710419557 23.370092647312628 88-89 22.73725356409203 26.80222086325185 27.04476228209985 23.415763290556267 90-91 22.726549371403753 26.92556974868303 27.110759249788064 23.237121630125152 92-93 22.70295582174275 26.74620826228852 27.068792827958948 23.482043088009778 94-95 22.49338108683005 26.770635231219796 27.249333104374294 23.486650577575865 96-97 22.567374521597753 26.59366732472072 27.28697614891248 23.551982004769044 98-99 22.382755120764124 26.70527312529685 27.116198509010026 23.795773244929006 100-101 22.315721164275615 26.691872055694958 27.357871478828173 23.634535301201254 102-103 22.292666758561204 26.572583435761427 27.36362955213995 23.771120253537415 104-105 22.225916381489487 26.624330171466664 27.23768454291786 23.912068904125984 106-107 22.19692100327271 26.662722022129486 27.223825538584144 23.91653143601366 108-109 22.191214182103366 26.6111181410618 27.232375139139403 23.96529253769543 110-111 22.08042762196052 26.7871545835829 27.255938475009657 23.876479319446926 112-113 22.196901087841123 26.774768652683505 27.242132346495506 23.786197912979862 114-115 22.111993002082155 26.79745713331943 27.18933080694311 23.901219057655304 116-117 21.98321847821329 26.70249219445806 27.40348178811784 23.910807539210815 118-119 21.96622770207727 26.642243022623962 27.293780938796015 24.097748336502754 120-121 21.9169612989271 26.67191729769432 27.42288064104651 23.988240762332076 122-123 21.92313165122297 26.740091854180235 27.343858261692173 23.99291823290462 124-125 21.959525846995838 26.705617148501137 27.237161702973626 24.0976953015294 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 653.0 1 529.0 2 353.0 3 289.5 4 263.5 5 227.0 6 204.0 7 199.0 8 213.5 9 229.0 10 221.5 11 245.5 12 263.5 13 262.0 14 311.5 15 389.0 16 475.5 17 596.5 18 778.0 19 1039.0 20 1429.5 21 1968.5 22 2775.5 23 4136.5 24 5896.5 25 8397.0 26 12592.5 27 18012.0 28 23745.5 29 30043.5 30 37190.5 31 45734.0 32 54152.5 33 61030.0 34 67784.5 35 75204.5 36 84744.5 37 93436.0 38 97894.5 39 102067.0 40 107749.0 41 112242.0 42 116622.5 43 121713.0 44 126815.5 45 132422.0 46 135855.5 47 137675.5 48 139638.0 49 140668.5 50 139557.5 51 135978.0 52 131218.5 53 126511.0 54 122512.5 55 114430.0 56 103156.0 57 92822.5 58 83572.0 59 72764.5 60 60295.5 61 49772.0 62 40011.5 63 31061.0 64 22506.0 65 15453.0 66 11272.5 67 9061.0 68 7446.0 69 5740.0 70 4289.0 71 3044.5 72 2275.0 73 1771.0 74 1222.5 75 770.0 76 491.5 77 276.0 78 150.5 79 100.0 80 65.5 81 55.5 82 45.0 83 33.0 84 32.5 85 27.5 86 22.5 87 20.0 88 15.0 89 14.5 90 12.5 91 12.0 92 9.5 93 7.0 94 6.0 95 5.0 96 4.0 97 2.5 98 3.0 99 2.5 100 4.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06253955190568337 2 0.050500616673725116 3 0.04532473240535352 4 0.0452675403139903 5 0.044295274760815524 6 0.004318002897923269 7 4.289406852241658E-4 8 0.0 9 9.72265553174776E-4 10-11 0.0037746780299726594 12-13 0.0024306638829369397 14-15 0.008106978950736733 16-17 0.0027309223625938558 18-19 0.00473264556030663 20-21 0.009651165417543732 22-23 0.016542812426811996 24-25 0.003474419550315743 26-27 0.01614246778726944 28-29 0.007492163968582097 30-31 0.0070489252605171245 32-33 0.0025307500428225784 34-35 0.018558833647365575 36-37 0.01585650733045333 38-39 0.026294064004241367 40-41 0.005704911113481406 42-43 0.001329716124194914 44-45 2.573644111344995E-4 46-47 0.002244789586006468 48-49 0.00297398875088755 50-51 0.0018301469236231074 52-53 0.0016299746038518303 54-55 0.005604824953595767 56-57 0.002516452019981773 58-59 0.0076923362883533734 60-61 0.015513354782273997 62-63 0.012296299643092755 64-65 0.0023448757458921065 66-67 0.0038032740756542703 68-69 0.010051510057086287 70-71 0.0023019816773696897 72-73 0.0029167966595243277 74-75 0.0010008615988563868 76-77 2.71662433975305E-4 78-79 0.002816710499638689 80-81 0.0025307500428225784 82-83 0.0014583983297621638 84-85 0.011152457815828312 86-87 0.00661998457529296 88-89 0.0016728686723742467 90-91 8.149873019259151E-4 92-93 0.0021304054032800237 94-95 1.4298022840805527E-5 96-97 0.001730060763737469 98-99 0.024406724989255037 100-101 0.014655473411825666 102-103 0.006434110278362487 104-105 0.013626015767287667 106-107 0.03168441861522505 108-109 0.001272524032831692 110-111 0.0019016370378271352 112-113 0.01619965987863266 114-115 0.03116968979295605 116-117 0.04901362229828135 118-119 0.35417632378959374 120-121 0.11648599208404263 122-123 0.019573993269062766 124-125 0.3176448754313356 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3496987.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.20020319956646 #Duplication Level Percentage of deduplicated Percentage of total 1 84.74843926149377 58.64609217741484 2 8.53486733178035 11.812291072810833 3 2.908117086823175 6.0372688000888495 4 1.3112791120367915 3.6296312401717215 5 0.6856686271620636 2.3724204163591285 6 0.44221836984202345 1.8360960630989434 7 0.28718622082832396 1.3911341386204905 8 0.19880917284854546 1.1006108127245662 9 0.13866129040300157 0.8635850524621622 >10 0.6836519277561686 8.209769026512845 >50 0.0444454831157765 2.1152614556842835 >100 0.01644394224076654 1.8755744617419654 >500 2.12173669262069E-4 0.11026528230926118 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.8596045681611057E-5 0.0 0.0 0.0 0.0 2 2.8596045681611057E-5 0.0 0.0 8.578813704483316E-5 0.0 3 2.8596045681611057E-5 0.0 0.0 8.578813704483316E-5 0.0 4 2.8596045681611057E-5 0.0 0.0 1.429802284080553E-4 0.0 5 1.1438418272644423E-4 0.0 0.0 1.7157627408966633E-4 0.0 6 1.1438418272644423E-4 0.0 0.0 1.7157627408966633E-4 0.0 7 1.429802284080553E-4 0.0 0.0 2.001723197712774E-4 0.0 8 1.7157627408966633E-4 0.0 0.0 2.2876836545288845E-4 0.0 9 1.7157627408966633E-4 0.0 0.0 2.2876836545288845E-4 0.0 10-11 2.2876836545288845E-4 0.0 0.0 3.4315254817933265E-4 0.0 12-13 3.2885452533852713E-4 0.0 0.0 3.7174859386094374E-4 0.0 14-15 3.8604661670174926E-4 0.0 0.0 4.146426623833603E-4 0.0 16-17 4.289406852241658E-4 0.0 0.0 4.289406852241658E-4 0.0 18-19 5.576228907914156E-4 0.0 0.0 4.4323870806497133E-4 0.0 20-21 6.434110278362487E-4 0.0 0.0 5.004307994281934E-4 0.0 22-23 7.291991648810819E-4 0.0 0.0 5.4332486795061E-4 0.0 24-25 9.150734618115538E-4 0.0 0.0 7.434971877218875E-4 0.0 26-27 9.150734618115538E-4 0.0 0.0 0.0011152457815828312 0.0 28-29 0.0012010339186276642 0.0 0.0 0.0026308362027082175 1.4298022840805528E-5 30-31 0.0013154181013541085 1.4298022840805528E-5 0.0 0.009279416823682787 2.8596045681611057E-5 32-33 0.0013297161241949142 2.8596045681611057E-5 0.0 0.023448757458921065 2.8596045681611057E-5 34-35 0.0014583983297621638 2.8596045681611057E-5 0.0 0.045624990885010444 4.289406852241658E-5 36-37 0.001544186466806997 2.8596045681611057E-5 0.0 0.08124136578145702 5.719209136322211E-5 38-39 0.0019016370378271352 2.8596045681611057E-5 0.0 0.13274284405403852 5.719209136322211E-5 40-41 0.0019159350606679406 2.8596045681611057E-5 0.0 0.18844794104181686 5.719209136322211E-5 42-43 0.0020732133119168014 2.8596045681611057E-5 0.0 0.2437383953672118 5.719209136322211E-5 44-45 0.0022590876088472733 2.8596045681611057E-5 0.0 0.31397028356124856 5.719209136322211E-5 46-47 0.0026451342255490225 2.8596045681611057E-5 0.0 0.39608382873599474 5.719209136322211E-5 48-49 0.0028024124767978835 2.8596045681611057E-5 0.0 0.4840595632754711 5.719209136322211E-5 50-51 0.0028024124767978835 2.8596045681611057E-5 0.0 0.5788125606414893 5.719209136322211E-5 52-53 0.0028024124767978835 2.8596045681611057E-5 0.0 0.671692517015362 5.719209136322211E-5 54-55 0.002902498636683522 2.8596045681611057E-5 0.0 0.7777981445169799 5.719209136322211E-5 56-57 0.0030597768879323827 2.8596045681611057E-5 0.0 0.9051649319828756 5.719209136322211E-5 58-59 0.0030597768879323827 2.8596045681611057E-5 0.0 1.0455715162795858 5.719209136322211E-5 60-61 0.0030597768879323827 2.8596045681611057E-5 0.0 1.200833746307893 5.719209136322211E-5 62-63 0.0030597768879323827 2.8596045681611057E-5 0.0 1.3557814198337026 5.719209136322211E-5 64-65 0.0031312670021364105 2.8596045681611057E-5 0.0 1.5186073039447958 5.719209136322211E-5 66-67 0.0033171412990668824 2.8596045681611057E-5 0.0 1.7059971912963932 7.149011420402765E-5 68-69 0.0033743333904301047 2.8596045681611057E-5 0.0 1.9205104279769984 8.578813704483316E-5 70-71 0.0034029294361117156 2.8596045681611057E-5 0.0 2.1631335775626273 8.578813704483316E-5 72-73 0.003474419550315743 2.8596045681611057E-5 0.0 2.4115760224444642 8.578813704483316E-5 74-75 0.0035173136188381597 2.8596045681611057E-5 0.0 2.664150595927294 8.578813704483316E-5 76-77 0.0035173136188381597 2.8596045681611057E-5 0.0 2.942847657140275 8.578813704483316E-5 78-79 0.0035173136188381597 2.8596045681611057E-5 0.0 3.2559743573539164 8.578813704483316E-5 80-81 0.003903360235539909 2.8596045681611057E-5 0.0 3.606547579387627 8.578813704483316E-5 82-83 0.004003446395425548 2.8596045681611057E-5 0.0 3.973549229665424 8.578813704483316E-5 84-85 0.00418932069235602 2.8596045681611057E-5 0.0 4.337948639786193 8.578813704483316E-5 86-87 0.004375194989286492 2.8596045681611057E-5 0.0 4.723623507894081 8.578813704483316E-5 88-89 0.004403791034968103 2.8596045681611057E-5 0.0 5.146172976908407 8.578813704483316E-5 90-91 0.004561069286216963 2.8596045681611057E-5 0.0 5.609285936722098 8.578813704483316E-5 92-93 0.004646857423261796 2.8596045681611057E-5 0.0 6.103911738876924 8.578813704483316E-5 94-95 0.004661155446102602 2.8596045681611057E-5 0.0 6.6009968009603694 8.578813704483316E-5 96-97 0.004661155446102602 2.8596045681611057E-5 0.0 7.097366961901774 8.578813704483316E-5 98-99 0.005047202062804351 2.8596045681611057E-5 0.0 7.635730415926625 8.578813704483316E-5 100-101 0.005390354610983684 2.8596045681611057E-5 0.0 8.232029458502419 8.578813704483316E-5 102-103 0.0056906130906406 2.8596045681611057E-5 0.0 8.857067526988232 8.578813704483316E-5 104-105 0.006491302369725709 2.8596045681611057E-5 0.0 9.48483651783664 8.578813704483316E-5 106-107 0.0067343687580194034 2.8596045681611057E-5 0.0 10.106185696429527 8.578813704483316E-5 108-109 0.006805858872223431 2.8596045681611057E-5 0.0 10.744478032088766 8.578813704483316E-5 110-111 0.006977435146313098 2.8596045681611057E-5 0.0 11.424334720146229 8.578813704483316E-5 112-113 0.00706322328335793 2.8596045681611057E-5 0.0 12.15279038783959 8.578813704483316E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2800 0.0 46.95754 1 GTATCAA 4500 0.0 42.30665 1 TCAACGC 4500 0.0 40.18729 4 ATCAACG 4565 0.0 39.875698 3 CAACGCA 4540 0.0 39.572853 5 AACGCAG 5045 0.0 35.364677 6 TACATGG 10350 0.0 34.314217 2 GTACATG 10805 0.0 33.752567 1 TATCAAC 5670 0.0 32.315266 2 ACATGGG 10735 0.0 31.80808 3 CGCAGAG 5905 0.0 29.709347 8 ACGCAGA 6030 0.0 29.291143 7 CATGGGG 6685 0.0 27.141014 4 GAGTACA 7695 0.0 22.575914 1 TATACCG 270 4.5563866E-8 22.03345 5 GTATAGG 1480 0.0 21.305183 1 AGTACAT 7735 0.0 20.842463 2 TACCGCG 150 0.004721589 19.8233 7 CAGAGTA 7060 0.0 19.628437 10-11 GCAGAGT 8765 0.0 19.40656 9 >>END_MODULE