FastQCFastQC Report
Thu 26 May 2016
SRR1364194_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364194_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3496987
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41530.11875937771573071No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39600.11324034089917978No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39250.060.977861
GTATCAA72650.049.1701351
ATCAACG84900.041.563813
TCAACGC86300.040.8889544
CAACGCA86600.040.7391475
AACGCAG95200.037.4333656
TATCAAC96750.036.536642
CGCAGAG111450.031.8143278
ACGCAGA112500.031.6231547
TACATGG102300.031.296792
GTACATG106550.031.0675581
ACATGGG106850.028.9599063
GCAGAGT144100.024.3168819
AGAGTAC119550.024.06043210-11
CATGGGG67200.023.5545314
CAGAGTA128400.022.0082310-11
GAGTACT84150.021.88079312-13
AGTACTT92800.020.99519312-13
GAGTACA80550.019.9564821
GTACTTT91000.019.90684514-15
Produced by FastQC (version 0.11.5)