FastQCFastQC Report
Thu 26 May 2016
SRR1364193_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364193_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1259916
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39530.3137510754685233No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17050.045.7126731
GTATCAA28900.039.938671
ATCAACG30850.035.291363
CAACGCA31200.034.8913085
TCAACGC30900.034.849184
GTACATG73250.034.8449131
TACATGG75000.033.3959122
AACGCAG32950.033.2068756
ACATGGG75100.032.7176863
TATCAAC35200.031.944172
GTCTACG800.00536404229.7481331
CATGGGA52550.028.4166874
ATGGGAT21800.027.0146565
CGCAGAG41100.026.4773738
ACGCAGA41350.026.3172917
CATGGGT11950.025.8885614
GAGTACA53500.025.6890431
CTGGTCG4700.025.3055049
CATGGGG22650.024.1652954
AGTACAT51250.024.145822
Produced by FastQC (version 0.11.5)