##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364193_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1259916 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.177406271529215 31.0 31.0 34.0 26.0 34.0 2 30.138722740246177 31.0 31.0 34.0 25.0 34.0 3 30.216992243927372 31.0 31.0 34.0 26.0 34.0 4 33.47217274802447 37.0 35.0 37.0 27.0 37.0 5 33.67494817114792 37.0 35.0 37.0 28.0 37.0 6 33.673039313732026 37.0 35.0 37.0 28.0 37.0 7 33.559035681743865 37.0 35.0 37.0 28.0 37.0 8 33.5007794170405 37.0 35.0 37.0 27.0 37.0 9 34.798291314659075 39.0 35.0 39.0 27.0 39.0 10-11 34.80223641893586 39.0 35.0 39.0 27.0 39.0 12-13 34.78414791144806 39.0 35.0 39.0 27.0 39.0 14-15 35.99169547811124 40.0 36.0 41.0 27.0 41.0 16-17 36.05227848523235 40.0 36.0 41.0 27.0 41.0 18-19 35.95787020722017 40.0 36.0 41.0 26.5 41.0 20-21 35.92440567466403 40.0 36.0 41.0 26.0 41.0 22-23 35.980285590467936 40.0 36.0 41.0 26.5 41.0 24-25 35.880992066137736 40.0 36.0 41.0 26.0 41.0 26-27 35.91164569701472 40.0 36.0 41.0 27.0 41.0 28-29 36.00642423780633 40.0 36.0 41.0 27.0 41.0 30-31 35.9119342083123 40.0 36.0 41.0 27.0 41.0 32-33 35.7225438045076 40.0 35.0 41.0 26.5 41.0 34-35 35.67303971058388 40.0 35.0 41.0 26.0 41.0 36-37 35.70797973833176 40.0 35.0 41.0 26.5 41.0 38-39 35.55423972709292 40.0 35.0 41.0 25.5 41.0 40-41 35.56979274808796 40.0 35.0 41.0 26.0 41.0 42-43 35.50389867260992 40.0 35.0 41.0 26.0 41.0 44-45 35.50192195352706 40.0 35.0 41.0 26.0 41.0 46-47 35.449679581813385 40.0 35.0 41.0 26.0 41.0 48-49 35.38751392950006 40.0 35.0 41.0 26.0 41.0 50-51 35.297287676321275 40.0 35.0 41.0 26.0 41.0 52-53 35.17382508040219 39.0 35.0 41.0 26.0 41.0 54-55 35.02085734287048 39.0 34.0 41.0 26.0 41.0 56-57 34.857202781772756 39.0 34.0 41.0 25.5 41.0 58-59 34.66012694497093 39.0 34.0 41.0 25.0 41.0 60-61 34.44108337381222 38.0 34.0 40.0 24.0 41.0 62-63 34.174989840592545 38.0 33.5 40.0 23.5 41.0 64-65 33.89295794322796 37.5 33.0 40.0 23.0 41.0 66-67 33.606500353991855 37.0 33.0 40.0 22.0 41.0 68-69 33.38800364468743 37.0 33.0 39.5 22.0 41.0 70-71 33.22663336285911 36.0 33.0 39.0 22.0 41.0 72-73 32.911259560161156 36.0 33.0 39.0 22.0 41.0 74-75 32.55970001174681 35.5 32.5 38.5 20.5 40.0 76-77 32.212766168538224 35.0 32.0 37.5 20.0 39.5 78-79 31.84448328301252 35.0 32.0 37.0 18.5 39.0 80-81 31.514835512843714 35.0 32.0 36.5 18.0 39.0 82-83 31.200699887929037 35.0 32.0 36.0 15.5 38.0 84-85 30.907600189218964 35.0 32.0 36.0 12.0 37.0 86-87 30.66227550090641 35.0 31.0 35.0 7.5 37.0 88-89 30.464042444099448 35.0 31.0 35.0 7.0 36.0 90-91 30.283570888852907 35.0 31.0 35.0 4.5 36.0 92-93 30.12443567666416 35.0 31.0 35.0 2.0 36.0 94-95 29.981112629730873 35.0 31.0 35.0 2.0 36.0 96-97 29.872380777766136 35.0 31.0 35.0 2.0 35.0 98-99 29.78796483257614 35.0 31.0 35.0 2.0 35.0 100-101 29.68197324266062 35.0 31.0 35.0 2.0 35.0 102-103 28.74828440943682 33.5 29.0 34.5 2.0 35.0 104-105 29.396855425282318 34.0 30.0 35.0 2.0 35.0 106-107 29.49559573812857 34.5 31.0 35.0 2.0 35.0 108-109 29.466192984294192 35.0 31.0 35.0 2.0 35.0 110-111 29.388462405430204 35.0 30.0 35.0 2.0 35.0 112-113 29.51442040580483 35.0 31.0 35.0 2.0 35.0 114-115 29.49535723016455 35.0 31.0 35.0 2.0 35.0 116-117 29.403768187720452 35.0 31.0 35.0 2.0 35.0 118-119 29.17680107245245 35.0 31.0 35.0 2.0 35.0 120-121 29.1035807149048 35.0 31.0 35.0 2.0 35.0 122-123 28.964274205581958 35.0 31.0 35.0 2.0 35.0 124-125 28.115250937364078 34.5 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7088015368649181 1101 2 0.7592565920518233 1101 3 0.5853071658465545 1101 4 0.6672971432788444 1101 5 0.42265234349184055 1101 6 0.4534746175942388 1101 7 0.47976257838377023 1101 8 0.4614385125602354 1101 9 0.601480807871468 1101 10-11 0.6722124640673783 1101 12-13 0.7919589156988565 1101 14-15 0.9293041680354079 1101 16-17 0.8438550481568683 1101 18-19 1.0249879142640737 1101 20-21 1.0940312164437387 1101 22-23 1.0357392483910814 1101 24-25 1.1916869353926103 1101 26-27 1.1471497625425613 1101 28-29 1.097472122440351 1101 30-31 1.1065948987639729 1101 32-33 1.1994410062950749 1101 34-35 1.2175975289846832 1101 36-37 1.1398148677596964 1101 38-39 1.138197139644383 1101 40-41 1.0216072604885014 1101 42-43 0.9738367618736632 1101 44-45 0.8113564342768385 1101 46-47 0.670813599362944 1101 48-49 0.7548243941029185 1101 50-51 0.6419403732326074 1101 52-53 0.6746038791542404 1101 54-55 0.6994782533633526 1101 56-57 0.7467856634945846 1101 58-59 0.662621303876243 1101 60-61 0.6168191582980995 1101 62-63 0.5008126954725256 1101 64-65 0.5611639720218164 1101 66-67 0.6017566860934949 1101 68-69 0.5754744397701756 1101 70-71 0.6987742297940258 1101 72-73 0.6169710540747673 1101 74-75 0.5444770095245843 1101 76-77 0.5555191837949636 1101 78-79 0.526007066967658 1101 80-81 0.46531028871958924 1101 82-83 0.44810625341569477 1101 84-85 0.4660804725970067 1101 86-87 0.4865517061453595 1101 88-89 0.37368347767473153 1101 90-91 0.3845255935432341 1101 92-93 0.39569400048930703 1101 94-95 0.48384236009055925 1101 96-97 0.38833156105493316 1101 98-99 0.42279328526209525 1101 100-101 0.43677303176766813 1101 102-103 0.26881080113009403 1101 104-105 0.3453419978837964 1101 106-107 0.29450597977728066 1101 108-109 0.3074035427800226 1101 110-111 0.3460976712649959 1101 112-113 0.3831857499598996 1101 114-115 0.45147214735887076 1101 116-117 0.4425335677182254 1101 118-119 0.2292862710353596 1101 120-121 0.1402727657127194 1101 122-123 0.28067703625016804 1101 124-125 0.2992554732807342 1102 1 0.22387481243356788 1102 2 0.19884800903697553 1102 3 0.12532914365480252 1102 4 0.2445756390631857 1102 5 0.0986364825160635 1102 6 0.14701304472224308 1102 7 0.06556112162461858 1102 8 0.17455274853965363 1102 9 0.2855631857536238 1102 10-11 0.29392612531739104 1102 12-13 0.2719382021008201 1102 14-15 0.30805850768006593 1102 16-17 0.2892642632768272 1102 18-19 0.48175314070357445 1102 20-21 0.5575824558559788 1102 22-23 0.4447920875154736 1102 24-25 0.6966939371722276 1102 26-27 0.6503005633711112 1102 28-29 0.39647850183956024 1102 30-31 0.487031610455972 1102 32-33 0.6742358152534536 1102 34-35 0.6429209966160414 1102 36-37 0.4234258411043186 1102 38-39 0.6388456551698596 1102 40-41 0.5348462156673861 1102 42-43 0.5181813661272443 1102 44-45 0.34370137403235645 1102 46-47 0.35559250844678303 1102 48-49 0.37427159387731024 1102 50-51 0.3821469986665704 1102 52-53 0.3624339109539889 1102 54-55 0.5315577089749866 1102 56-57 0.45333816225468127 1102 58-59 0.5608870262751324 1102 60-61 0.5542061087313286 1102 62-63 0.49025593243414534 1102 64-65 0.5056402243192792 1102 66-67 0.5404364130240964 1102 68-69 0.6512474313777687 1102 70-71 0.692054466298579 1102 72-73 0.5607453050302169 1102 74-75 0.5629612214840165 1102 76-77 0.4577112513621344 1102 78-79 0.627591219606451 1102 80-81 0.7538741702339173 1102 82-83 0.5980871687038878 1102 84-85 0.5929731411502637 1102 86-87 0.5002191556498907 1102 88-89 0.5257059708917566 1102 90-91 0.515182934228438 1102 92-93 0.5856075479604002 1102 94-95 0.5425460827033888 1102 96-97 0.4694950720584288 1102 98-99 0.5293662058794162 1102 100-101 0.452698433640645 1102 102-103 0.41740473194862204 1102 104-105 0.5033372765449009 1102 106-107 0.45791305060222953 1102 108-109 0.4135771431534465 1102 110-111 0.4383345696711167 1102 112-113 0.4863665757809059 1102 114-115 0.5435230609938628 1102 116-117 0.6479330824559995 1102 118-119 0.3786600007472174 1102 120-121 0.42604322889989277 1102 122-123 0.5061543971627316 1102 124-125 0.43705681723343304 1103 1 0.41223813655810915 1103 2 0.056832620641976916 1103 3 0.2796153258925145 1103 4 0.39347835184285174 1103 5 0.38670132380291733 1103 6 0.3390566736762253 1103 7 0.35739689043018075 1103 8 0.27612723497094294 1103 9 0.49855450524406564 1103 10-11 0.49842134188454423 1103 12-13 0.4909639088373652 1103 14-15 0.6706114361477589 1103 16-17 0.6284604731409047 1103 18-19 0.6706626095402086 1103 20-21 0.7931501151772906 1103 22-23 0.7357500807377448 1103 24-25 0.9433375858765345 1103 26-27 0.9034744650000235 1103 28-29 0.8710403215672429 1103 30-31 0.9461031222953267 1103 32-33 1.1518556238679736 1103 34-35 1.076343577742918 1103 36-37 0.9538031084779774 1103 38-39 1.0693099022608976 1103 40-41 1.04842697403177 1103 42-43 0.9274325276632354 1103 44-45 0.9008251303036872 1103 46-47 0.8559549992038669 1103 48-49 0.7873659402257829 1103 50-51 0.7148844602196718 1103 52-53 0.8151436196848394 1103 54-55 0.770002713948692 1103 56-57 0.7961553657528952 1103 58-59 0.7871100110794202 1103 60-61 0.8148112214165764 1103 62-63 0.8103522815139499 1103 64-65 0.776427895057239 1103 66-67 0.8963740357109558 1103 68-69 0.9332879713324331 1103 70-71 0.8243958073457875 1103 72-73 0.7621595006981536 1103 74-75 0.8165716775961371 1103 76-77 0.8139901392260427 1103 78-79 0.8863277285675579 1103 80-81 0.826784338762014 1103 82-83 0.8113116685027713 1103 84-85 0.748670749192982 1103 86-87 0.8163004077724487 1103 88-89 0.75965100235981 1103 90-91 0.8702628026238237 1103 92-93 0.8817534643394396 1103 94-95 1.022087483724146 1103 96-97 1.0380281044802757 1103 98-99 0.8814618480420329 1103 100-101 0.8594287188401815 1103 102-103 0.8903788481817045 1103 104-105 0.8625139829611079 1103 106-107 0.8017097920391372 1103 108-109 0.7617216661710096 1103 110-111 0.5933690665074529 1103 112-113 0.7637760253489247 1103 114-115 0.8036858988268207 1103 116-117 0.8012806725548245 1103 118-119 0.6314822502131143 1103 120-121 0.6837809313106362 1103 122-123 0.766521361850593 1103 124-125 0.6628698924315977 1104 1 0.6469411072919264 1104 2 0.6895641719202033 1104 3 0.6725156998491499 1104 4 0.7354666603970657 1104 5 0.5250735323272124 1104 6 0.6952458850885179 1104 7 0.7061169307090012 1104 8 0.6121001896438898 1104 9 0.7329230524713566 1104 10-11 0.743462979745722 1104 12-13 0.7598167849791366 1104 14-15 0.9564578648889324 1104 16-17 0.8698287178216475 1104 18-19 0.9342065480928028 1104 20-21 1.0418691308394088 1104 22-23 1.0220166023262678 1104 24-25 1.2129787053385854 1104 26-27 1.1799038124131087 1104 28-29 0.9630393743660264 1104 30-31 1.0084903255158082 1104 32-33 1.1912139975052725 1104 34-35 1.126789157930702 1104 36-37 1.152905771940624 1104 38-39 1.2004524438055455 1104 40-41 1.1395239372101784 1104 42-43 1.062234516216563 1104 44-45 0.971232378442231 1104 46-47 0.8938584171259478 1104 48-49 0.971164387874552 1104 50-51 0.911521355130148 1104 52-53 0.8692066660581332 1104 54-55 0.7915052915712337 1104 56-57 0.9040381120330494 1104 58-59 0.8520812406963074 1104 60-61 0.8627356752887607 1104 62-63 0.7986851703723516 1104 64-65 0.8657458674887906 1104 66-67 0.9006270488667525 1104 68-69 0.6891273615261184 1104 70-71 0.8125643306084243 1104 72-73 0.7948016541692269 1104 74-75 0.7410984114112438 1104 76-77 0.6666600768866502 1104 78-79 0.7042961754962533 1104 80-81 0.7609231156941298 1104 82-83 0.8253605865502287 1104 84-85 0.8088241456977308 1104 86-87 0.8480677947351687 1104 88-89 0.9091390213174932 1104 90-91 0.9897880407831643 1104 92-93 0.9312545471600622 1104 94-95 0.9148092612975027 1104 96-97 0.938999184958508 1104 98-99 0.8979325615082558 1104 100-101 0.8548935091680292 1104 102-103 0.8592945390195545 1104 104-105 0.8885769587496988 1104 106-107 0.9625758948440541 1104 108-109 0.9373394033107161 1104 110-111 1.0185652165671222 1104 112-113 1.0613514691573833 1104 114-115 1.0134094259331974 1104 116-117 1.0293505309214872 1104 118-119 0.9672656507964099 1104 120-121 0.9656955357701662 1104 122-123 0.947177651616748 1104 124-125 1.0492488657030492 1105 1 0.5314041393600775 1105 2 0.5857858352387915 1105 3 0.5667383592948809 1105 4 0.631934121321855 1105 5 0.5247458959299323 1105 6 0.43607396428709677 1105 7 0.44688791961689844 1105 8 0.36589592058381726 1105 9 0.4671548126156395 1105 10-11 0.5889126726533931 1105 12-13 0.6879682323023815 1105 14-15 0.7424318491528226 1105 16-17 0.7371778806333182 1105 18-19 0.8134050397842003 1105 20-21 0.8985359409348845 1105 22-23 0.8522922303124361 1105 24-25 1.1241715239987329 1105 26-27 1.0325025877560634 1105 28-29 0.8854688559046267 1105 30-31 0.9003617368074828 1105 32-33 1.1417515883681233 1105 34-35 1.1745274684124567 1105 36-37 1.0367575441723034 1105 38-39 0.9412671644385711 1105 40-41 0.8930464503303952 1105 42-43 0.8621622955926753 1105 44-45 0.834965026017116 1105 46-47 0.8470401602697066 1105 48-49 0.7817326162559652 1105 50-51 0.6033658243043405 1105 52-53 0.6891060634040258 1105 54-55 0.75852678986233 1105 56-57 0.74980756681515 1105 58-59 0.7819178359101144 1105 60-61 0.6019614297032092 1105 62-63 0.7633502176996387 1105 64-65 0.7111620936743321 1105 66-67 0.8611506596392076 1105 68-69 0.8121095501991249 1105 70-71 0.7665186206906611 1105 72-73 0.7868613228036807 1105 74-75 0.884715505987927 1105 76-77 0.7532975459475111 1105 78-79 0.8221437534195815 1105 80-81 0.8344686280457445 1105 82-83 0.8267189417423886 1105 84-85 0.860603211334972 1105 86-87 0.7933430950849356 1105 88-89 0.8953394824833012 1105 90-91 0.780213802172188 1105 92-93 0.9085839364823798 1105 94-95 0.8849947709942789 1105 96-97 0.8906039142844406 1105 98-99 0.8304675869640583 1105 100-101 0.930749350278159 1105 102-103 0.906036159894466 1105 104-105 0.9644475645901771 1105 106-107 1.0099493055614523 1105 108-109 0.9129733797394586 1105 110-111 0.8440833672016801 1105 112-113 0.8805761886345351 1105 114-115 0.9944778074459535 1105 116-117 0.9595129345183437 1105 118-119 1.0661643144055084 1105 120-121 0.9997280986095625 1105 122-123 0.9535172049794411 1105 124-125 0.812713385774785 1106 1 0.2562791713484778 1106 2 0.3995493769943117 1106 3 0.4205482486861065 1106 4 0.38962896621366383 1106 5 0.22576724996331876 1106 6 0.403604744235345 1106 7 0.197564367625084 1106 8 0.28161743669175365 1106 9 0.5322487874230006 1106 10-11 0.533109408415001 1106 12-13 0.5346602053468175 1106 14-15 0.5424585772372197 1106 16-17 0.5393755547212962 1106 18-19 0.5427165072697733 1106 20-21 0.6941886464425764 1106 22-23 0.6623071582319113 1106 24-25 0.8667078486027862 1106 26-27 0.8555080755436038 1106 28-29 0.8092631065230691 1106 30-31 0.9959146385106834 1106 32-33 0.9591790102453501 1106 34-35 1.0312939388836 1106 36-37 0.8655125556881273 1106 38-39 0.9923600303147708 1106 40-41 0.7544613327552625 1106 42-43 0.8349713035258048 1106 44-45 0.7454327674939876 1106 46-47 0.6980699023721186 1106 48-49 0.5751688811483433 1106 50-51 0.6052220044630019 1106 52-53 0.6283856846613816 1106 54-55 0.7019721536770476 1106 56-57 0.6587253637385686 1106 58-59 0.4842066413922126 1106 60-61 0.5544420234078871 1106 62-63 0.5169896444675288 1106 64-65 0.4736456729629168 1106 66-67 0.4047211336362011 1106 68-69 0.5037810506682803 1106 70-71 0.5503700239150859 1106 72-73 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-3.6032122426820195 2216 116-117 -3.4797547741194315 2216 118-119 -3.2552453578142035 2216 120-121 -3.1506746522295934 2216 122-123 -3.1558797979697886 2216 124-125 -2.9947233066201093 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 35267.0 3 6530.0 4 4679.0 5 4072.0 6 4229.0 7 4322.0 8 4303.0 9 4418.0 10 4998.0 11 5654.0 12 6037.0 13 6080.0 14 6206.0 15 5973.0 16 5788.0 17 5234.0 18 4727.0 19 4268.0 20 4046.0 21 4134.0 22 4741.0 23 6452.0 24 10476.0 25 15430.0 26 17131.0 27 15305.0 28 16601.0 29 19950.0 30 23275.0 31 28119.0 32 35859.0 33 46781.0 34 63834.0 35 97106.0 36 185668.0 37 370937.0 38 170609.0 39 677.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.172408178825435 20.01771099312607 12.587194872548357 23.222685955500136 2 15.210140623163593 21.835978819172016 40.13763726402658 22.81624329363781 3 18.525135867407386 27.819308259404313 29.13970758729342 24.515848285894883 4 11.720382206573552 18.1066909972771 38.40043706161823 31.772489734531124 5 12.335558052464197 40.932873302179324 34.13031480900634 12.601253836350137 6 29.575461439427492 39.032370211975596 18.356613660861267 13.03555468773564 7 26.819412784384944 34.92251839811034 21.449037710570263 16.80903110693445 8 24.598862146365313 37.879191946129744 20.2173795713365 17.304566336168442 9 25.959696897300823 18.229577991321555 19.919311248564373 35.89141386281325 10-11 23.219157260250487 29.71031862887114 28.014861886214977 19.055662224663397 12-13 23.666290043047802 27.51729631071568 27.850383985439862 20.966029660796657 14-15 21.47802063253365 28.65074914282471 25.43696194614014 24.4342682785015 16-17 21.12010274031045 31.309363504568505 26.350381446420695 21.220152308700342 18-19 21.132943948508178 30.259344263466183 28.043724235534295 20.563987552491348 20-21 22.39008977293429 29.28108227604691 27.669940097135274 20.658887853883527 22-23 22.00146623782981 29.43660982315838 27.24277032420368 21.31915361480813 24-25 21.484303067632695 29.318204187118607 28.01215051783367 21.18534222741502 26-27 21.38354644666676 29.438377070685622 27.735071316724845 21.44300516592277 28-29 21.300775191337163 29.36432087809509 27.832512315270936 21.502391615296812 30-31 21.58045217463088 29.22402591475152 27.876614413294565 21.318907497323035 32-33 21.300333999003083 29.311071495471015 27.889154874575755 21.49943963095015 34-35 21.615959462286245 29.24115232087223 27.878648832845837 21.264239383995683 36-37 21.676025372697715 29.070243284018698 27.662051416189797 21.591679927093782 38-39 21.327326519100374 29.366382720445362 27.850547349709565 21.455743410744702 40-41 21.539277272157214 29.154661154846895 27.93938585754676 21.36667571544913 42-43 21.310072747866002 29.117798681561506 27.880715882496975 21.691412688075516 44-45 21.51545937622776 29.27864181313819 27.737347360734653 21.468551449899397 46-47 21.497347383259463 29.21549375817533 27.493039063789798 21.79411979477541 48-49 21.21476095890275 29.245184551672683 27.819926937341133 21.720127552083436 50-51 21.33158450340903 29.18568287707657 27.71273682623086 21.76999579328354 52-53 21.479902277493185 29.35211216181679 27.56843036001441 21.599555200675614 54-55 21.42004995860595 29.111167026367575 27.757030042632298 21.71175297239418 56-57 21.342202932968434 29.41806547269431 27.56071193855942 21.679019655777836 58-59 21.36117766637681 29.125144316996128 27.716642185529842 21.797035831097222 60-61 21.42113209823817 29.24155800443593 27.67663930066666 21.660670596659244 62-63 21.32326412772073 29.064214007796004 27.836032350901785 21.77648951358148 64-65 21.212869234063174 28.988676378901783 27.818453775869852 21.980000611165195 66-67 21.227370573331726 29.332253322580847 27.376446981578965 22.063929122508462 68-69 21.395124731403005 29.00335532911516 27.645586118923276 21.955933820558553 70-71 21.060305647671765 28.91401734448147 27.979088517813572 22.046588490033194 72-73 21.33952918363759 28.806417776994632 27.83469477738078 22.019358261986998 74-75 21.11768655756858 29.038515571796626 27.6915103190238 22.152287551610993 76-77 21.05391404009642 28.890209085865454 28.021577680363645 22.034299193674485 78-79 21.195409524557903 28.874101643067 27.7591448955626 22.171343936812505 80-81 21.055060799441215 28.885846271073433 27.77447058450011 22.284622344985237 82-83 21.296496930299746 28.92887899388441 27.793466915893784 21.981157159922056 84-85 21.217972506613375 28.693974832457837 27.819679581189238 22.268373079739554 86-87 21.055069247328216 28.62929342960014 28.003773519008885 22.311863804062764 88-89 21.37734275103441 28.451492463272793 27.96887364602027 22.20229113967253 90-91 21.342521856806666 28.526714315761904 27.989927812017573 22.14083601541386 92-93 21.293791660515314 28.501983512794016 28.03372686100171 22.17049796568896 94-95 21.134385149486157 28.413283107762737 28.006827439289605 22.4455043034615 96-97 21.32213398735609 28.38399231682005 27.88613336825688 22.40774032756698 98-99 21.046388941154614 28.354169494970183 27.921091721188418 22.67834984268678 100-101 20.855009837530904 28.51212951485513 27.956964920281667 22.6758957273323 102-103 21.098493928565194 28.321786838583495 27.964001606560863 22.61571762629045 104-105 20.888569949798846 28.52106638554867 28.04485398481582 22.545509679836666 106-107 20.61883458985396 28.72298573279084 27.99249541384196 22.665684263513235 108-109 20.640223382098956 28.84525328913949 27.645717451488366 22.86880587727319 110-111 20.68500783396751 28.960955569419113 27.676962738253412 22.67707385835996 112-113 20.756001360648444 28.859123270859143 27.842923695327475 22.541951673164938 114-115 20.527743052674662 28.949408711662727 27.757820492411444 22.765027743251167 116-117 20.511626281805412 28.90366477428392 27.783601308388505 22.80110763552216 118-119 20.413641882853504 28.837252460215023 27.796775944511104 22.952329712420365 120-121 20.44164015294787 28.830927762962588 27.803964820718523 22.92346726337102 122-123 20.613544320729847 28.785164621214616 27.75997100815336 22.841320049902176 124-125 20.388568662351005 28.772457271022322 27.801573049005608 23.03740101762106 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3015.0 1 2309.5 2 1394.0 3 969.0 4 633.0 5 498.5 6 451.0 7 435.5 8 457.0 9 476.5 10 445.0 11 400.5 12 393.0 13 390.5 14 441.5 15 506.0 16 585.0 17 695.5 18 851.0 19 1036.0 20 1303.5 21 1757.0 22 2333.0 23 3104.0 24 3964.5 25 5270.5 26 7641.0 27 10555.0 28 12650.5 29 14685.0 30 17687.0 31 20958.0 32 24926.5 33 29235.0 34 33970.5 35 37890.0 36 42255.5 37 46509.0 38 46588.0 39 45700.0 40 45516.0 41 45837.0 42 46267.0 43 46559.5 44 47632.5 45 47973.0 46 46981.0 47 45578.0 48 44584.5 49 43531.0 50 41964.0 51 39960.5 52 37685.5 53 35727.5 54 33175.5 55 29936.0 56 27031.0 57 24389.5 58 22154.0 59 19388.0 60 15808.0 61 12267.5 62 9379.5 63 7046.5 64 4980.0 65 3577.5 66 2687.0 67 2108.0 68 1573.5 69 1131.0 70 860.0 71 628.0 72 520.0 73 494.0 74 378.0 75 250.0 76 166.5 77 98.5 78 67.0 79 44.0 80 24.0 81 12.5 82 12.5 83 13.5 84 11.5 85 8.5 86 5.5 87 3.0 88 5.0 89 6.0 90 3.5 91 3.0 92 2.5 93 1.5 94 2.0 95 1.5 96 0.5 97 0.5 98 0.5 99 0.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06436937065645647 2 0.05270192616015671 3 0.047622222433876545 4 0.04738411132170716 5 0.04611418539013712 6 0.004286000019048889 7 3.1748148289251026E-4 8 0.0 9 0.0010318148194006585 10-11 0.003889148165433251 12-13 0.002976388902117284 14-15 0.008691055594182468 16-17 0.003214500014286667 18-19 0.005198759282364856 20-21 0.010040351896475638 22-23 0.018215500080957778 24-25 0.004127259277602634 26-27 0.017342426003003376 28-29 0.007778296330866502 30-31 0.007381444477250864 32-33 0.002539851863140082 34-35 0.019802907495420328 36-37 0.017104314890833992 38-39 0.0275018334555637 40-41 0.00619088891640395 42-43 0.001309611116931605 44-45 7.937037072312756E-5 46-47 0.002222370380247572 48-49 0.0030557592728404116 50-51 0.002063629638801317 52-53 0.0017461481559088068 54-55 0.005794037062788313 56-57 0.002539851863140082 58-59 0.008135462999120576 60-61 0.01603281488607177 62-63 0.013294537096123868 64-65 0.002182685194886008 66-67 0.004127259277602634 68-69 0.010238777823283458 70-71 0.0026985926045863372 72-73 0.003214500014286667 74-75 7.937037072312758E-4 76-77 2.3811111216938272E-4 78-79 0.003690722238625432 80-81 0.002857333346032593 82-83 0.001666777785185679 84-85 0.01258020375961572 86-87 0.006667111140742716 88-89 0.001508037043739424 90-91 8.730740779544034E-4 92-93 0.0023811111216938273 94-95 0.0 96-97 0.001666777785185679 98-99 0.02595411122646272 100-101 0.01583438895926395 102-103 0.006508370399296461 104-105 0.014604148213055473 106-107 0.03329587051835201 108-109 9.524444486775309E-4 110-111 0.0014286666730162964 112-113 0.017659907485895885 114-115 0.03337524088907514 116-117 0.052146333565094814 118-119 0.348078760806276 120-121 0.11492829680708873 122-123 0.019961648236866584 124-125 0.3133145384295461 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1259916.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.71258110550163 #Duplication Level Percentage of deduplicated Percentage of total 1 86.79668511577381 57.904308954747464 2 8.124691987464432 10.840383463418805 3 2.22926691365193 4.4616044934844705 4 0.8912410386725544 2.3782796030797724 5 0.45175036463329504 1.5068716420019315 6 0.26160700946447013 1.0471487301999716 7 0.18180727819867418 0.8490182954679684 8 0.12694407369490635 0.6775013449787373 9 0.10032644874930531 0.6023732714293484 >10 0.7017456574952123 9.684900742740586 >50 0.08586724471553676 3.9524557333608947 >100 0.047600198869496055 5.39435737025168 >500 2.3333430817909818E-4 0.08545917343542715 >1k 2.3333430817909818E-4 0.6153371814029936 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3953 0.3137510754685233 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 7.937037072312758E-5 0.0 0.0 0.0 3 0.0 7.937037072312758E-5 0.0 0.0 0.0 4 0.0 7.937037072312758E-5 0.0 0.0 0.0 5 0.0 1.5874074144625516E-4 0.0 0.0 0.0 6 0.0 1.5874074144625516E-4 0.0 0.0 0.0 7 0.0 2.3811111216938272E-4 0.0 0.0 0.0 8 0.0 2.3811111216938272E-4 0.0 0.0 0.0 9 0.0 2.3811111216938272E-4 0.0 0.0 0.0 10-11 0.0 3.9685185361563785E-4 0.0 0.0 0.0 12-13 0.0 3.9685185361563785E-4 0.0 0.0 0.0 14-15 0.0 4.7622222433876544E-4 0.0 0.0 0.0 16-17 0.0 4.7622222433876544E-4 0.0 3.968518536156379E-5 0.0 18-19 7.937037072312758E-5 4.7622222433876544E-4 0.0 1.5874074144625516E-4 0.0 20-21 7.937037072312758E-5 5.55592595061893E-4 0.0 3.1748148289251026E-4 0.0 22-23 7.937037072312758E-5 5.55592595061893E-4 0.0 8.730740779544033E-4 0.0 24-25 7.937037072312758E-5 5.952777804234568E-4 0.0 0.0017064629705472428 0.0 26-27 7.937037072312758E-5 6.349629657850206E-4 0.0 0.0030557592728404116 0.0 28-29 7.937037072312758E-5 6.349629657850206E-4 0.0 0.0055559259506189305 0.0 30-31 7.937037072312758E-5 7.937037072312757E-4 0.0 0.015477222291009876 0.0 32-33 7.937037072312758E-5 7.937037072312757E-4 0.0 0.03702627794233901 0.0 34-35 7.937037072312758E-5 7.937037072312757E-4 0.0 0.07004435216316009 0.0 36-37 7.937037072312758E-5 7.937037072312757E-4 0.0 0.12413525981097151 0.0 38-39 7.937037072312758E-5 8.730740779544033E-4 0.0 0.19660040828118702 0.0 40-41 7.937037072312758E-5 8.730740779544033E-4 0.0 0.2702561123122494 0.0 42-43 7.937037072312758E-5 8.730740779544033E-4 0.0 0.34823750154772226 0.0 44-45 7.937037072312758E-5 8.730740779544033E-4 0.0 0.4382038167623873 0.0 46-47 1.9842592680781892E-4 9.127592633159671E-4 0.0 0.5504732061502513 0.0 48-49 2.3811111216938272E-4 9.524444486775309E-4 0.0 0.667227021483972 0.0 50-51 2.3811111216938272E-4 9.524444486775309E-4 0.0 0.7902907812901812 0.0 52-53 2.3811111216938272E-4 9.921296340390947E-4 0.0 0.910219411452827 0.0 54-55 2.3811111216938272E-4 0.0010318148194006585 0.0 1.045784004647929 0.0 56-57 3.174814828925103E-4 0.001111185190123786 0.0 1.2056756164696694 0.0 58-59 3.174814828925103E-4 0.0011905555608469137 0.0 1.387751246908524 0.0 60-61 3.5716666825407405E-4 0.0011905555608469137 0.0 1.585978747789535 0.0 62-63 3.9685185361563785E-4 0.0011905555608469137 0.0 1.7842062486705461 0.0 64-65 3.9685185361563785E-4 0.0012302407462084775 0.0 1.986640379199883 0.0 66-67 3.9685185361563785E-4 0.0014286666730162962 0.0 2.2189177691211164 0.0 68-69 4.7622222433876544E-4 0.0015080370437394238 0.0 2.4965553259106166 0.0 70-71 5.159074097003292E-4 0.0015080370437394238 0.0 2.805425123579667 0.0 72-73 5.55592595061893E-4 0.0015080370437394238 0.0 3.115485476809565 0.0 74-75 5.55592595061893E-4 0.0015080370437394238 0.0 3.431538293029059 0.0 76-77 5.55592595061893E-4 0.0015080370437394238 0.0 3.781283831620521 0.0 78-79 5.55592595061893E-4 0.0015080370437394238 0.0 4.169920851866316 0.0 80-81 7.143333365081482E-4 0.0015080370437394238 0.0 4.605703872321646 0.0 82-83 7.143333365081482E-4 0.0015080370437394238 0.0 5.048312744659167 0.0 84-85 7.143333365081482E-4 0.0015080370437394238 0.0 5.482151191031783 0.0 86-87 7.143333365081482E-4 0.0015080370437394238 0.0 5.9367053041631355 0.0 88-89 7.143333365081482E-4 0.0015080370437394238 0.0 6.446977417542122 0.0 90-91 7.143333365081482E-4 0.0015080370437394238 0.0 7.005943253359748 0.0 92-93 7.143333365081482E-4 0.0015080370437394238 0.0 7.598919293032234 0.0 94-95 7.143333365081482E-4 0.0015080370437394238 0.0 8.191498480851104 0.0 96-97 7.143333365081482E-4 0.0015080370437394238 0.0 8.783918927928529 0.0 98-99 7.937037072312757E-4 0.0015080370437394238 0.0 9.423525060400852 0.0 100-101 8.333888925928395E-4 0.0015080370437394238 0.0 10.12769898945644 0.0 102-103 8.730740779544033E-4 0.0015080370437394238 0.0 10.85627930750939 0.0 104-105 0.001111185190123786 0.0015080370437394238 0.0 11.581208588509075 0.0 106-107 0.001111185190123786 0.0015080370437394238 0.0 12.288676387949673 0.0 108-109 0.001111185190123786 0.0015080370437394238 0.0 13.01844726156347 0.0 110-111 0.001111185190123786 0.0015080370437394238 0.0 13.812666876204446 0.0 112-113 0.0011508703754853499 0.0015080370437394238 0.0 14.646293879909454 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1705 0.0 45.712673 1 GTATCAA 2890 0.0 39.93867 1 ATCAACG 3085 0.0 35.29136 3 CAACGCA 3120 0.0 34.891308 5 TCAACGC 3090 0.0 34.84918 4 GTACATG 7325 0.0 34.844913 1 TACATGG 7500 0.0 33.395912 2 AACGCAG 3295 0.0 33.206875 6 ACATGGG 7510 0.0 32.717686 3 TATCAAC 3520 0.0 31.94417 2 GTCTACG 80 0.005364042 29.748133 1 CATGGGA 5255 0.0 28.416687 4 ATGGGAT 2180 0.0 27.014656 5 CGCAGAG 4110 0.0 26.477373 8 ACGCAGA 4135 0.0 26.317291 7 CATGGGT 1195 0.0 25.888561 4 GAGTACA 5350 0.0 25.689043 1 CTGGTCG 470 0.0 25.305504 9 CATGGGG 2265 0.0 24.165295 4 AGTACAT 5125 0.0 24.14582 2 >>END_MODULE