FastQCFastQC Report
Thu 26 May 2016
SRR1364193_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364193_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1259916
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67630.5367818172005118No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60650.4813812984357687No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35210.2794630753161322No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17860.14175548211150585No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12720.10095911155981827No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34450.072.7725751
GTATCAA62050.050.6718831
ATCAACG77150.040.4953423
TCAACGC77450.040.4153184
CAACGCA78150.040.045375
AACGCAG80300.039.488696
TATCAAC79750.039.1051562
TGGTATC15700.034.496512
ACGCAGA91850.034.3935057
CGCAGAG92500.034.023198
GTACATG71150.033.8128171
TACATGG73150.032.7886962
ACATGGG73850.031.9099713
GTGGTAT17550.030.877291
GCAGAGT102050.030.664359
CATGGGA53600.030.4205824
GAGTACT67800.029.2208512-13
CAGAGTA102000.027.15067310-11
GTACTTT74500.026.43321614-15
AGAGTAC98800.025.07951410-11
Produced by FastQC (version 0.11.5)