FastQCFastQC Report
Thu 26 May 2016
SRR1364192_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364192_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1727991
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17400.054.025331
GTATCAA28600.043.894091
ATCAACG30550.040.308673
TCAACGC31000.039.5327844
CAACGCA31550.038.8436245
AACGCAG33150.036.5940066
GTACATG74150.035.6255341
TATCAAC36700.033.879122
TACATGG77050.033.6642
ACATGGG75300.033.1813163
ACGCAGA42150.028.6392577
CATGGGG42700.028.4219684
CGCAGAG43700.027.4873738
TAGTACG1501.6887888E-423.7964344
CATGGGA34850.023.0453264
GAGTACA52850.022.5151371
GCAGAGT52500.022.2003619
GTGGTAT11850.021.0872781
GTGTAGC11900.020.9986761
CAGAGTA46800.020.90178510-11
Produced by FastQC (version 0.11.5)